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71.
Formalized African horse-sickness (AHS) type 9 virus cultivated in monkey kidney stable (MS) cell cultures was experimentally used for immunizing horses. Inactivated vaccines prepared either from viscerotropic or neurotropic type 9 AHS virus produced antibodies in vaccinated horses. Immunity developed in all horses vaccinated with various amounts of the vaccine, and protected them from infection, when challenged 5 weeks after vaccination. 相似文献
72.
Somfai T Kashiwazaki N Ozawa M Nakai M Maedomari N Noguchi J Kaneko H Nagai T Kikuchi K 《The Journal of reproduction and development》2008,54(3):149-155
The aim of the present study was to investigate the effects of centrifugation pretreatment on the viability and nuclear status of porcine in vitro matured (IVM) oocytes and on the developmental competence of in vitro fertilized (IVF) oocytes (zygotes) after cryopreservation by vitrification (Solid Surface Vitrification; SSV). Mature oocytes having the first polar body after IVM and zygotes having the second polar body at 10 h after IVF were centrifuged at 10,000 x g at 37 C for 20 min and then subjected to SSV. Their viability was evaluated by morphological appearance and fluorescein diacetate staining. The nuclear status of oocytes was evaluated 6 h after vitrification. The developmental ability to the blastocyst stage of vitrified zygotes was evaluated after 6 days of in vitro culture. Although centrifugation did not damage the oocytes directly, it drastically reduced the rate of live oocytes after SSV. The rates of vitrification-induced parthenogenetic activation were similar in both centrifuged and non-centrifuged oocytes (42.4 and 47.4%, respectively). Centrifugation had no significant effects on the viability of pronuclear oocytes. The development of vitrified zygotes to the blastocyst stage was significantly lower than that of the control irrespective of centrifugation pretreatment. There was no difference in the cleavage and blastocyst rates between the control and centrifuged zygotes after vitrification. There was also no difference in the total cell numbers of blastocysts between the control and centrifuged zygotes irrespective of vitrification. These results reveal that, in IVM porcine oocytes, centrifugation pretreatment is highly detrimental to cryotolerance; however, in zygotes, it has only a slight effect on viability and does not alter the developmental competence of surviving zygotes. 相似文献
73.
THORACOLUMBAR INTRADURAL DISC HERNIATION IN EIGHT DOGS: CLINICAL,LOW‐FIELD MAGNETIC RESONANCE IMAGING,AND COMPUTED TOMOGRAPHIC MYELOGRAPHY FINDINGS
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Shinji Tamura Shoko Doi Yumiko Tamura Kuniaki Takahashi Hirokazu Enomoto Tsuyoshi Ozawa Kazuyuki Uchida 《Veterinary radiology & ultrasound》2015,56(2):160-167
Intradural disc herniation is a rarely reported cause of neurologic deficits in dogs and few published studies have described comparative imaging characteristics. The purpose of this retrospective cross sectional study was to describe clinical and imaging findings in a group of dogs with confirmed thoracolumbar intradural disc herniation. Included dogs were referred to one of four clinics, had acute mono/paraparesis or paraplegia, had low field magnetic resonance imaging (MRI) and/or computed tomographic myelography, and were diagnosed with thoracolumbar intradural disc herniation during surgery. Eight dogs met inclusion criteria. The prevalence of thoracolumbar intradural disc herniation amongst the total population of dogs that developed a thoracolumbar intervertebral disc herniation and that were treated with a surgical procedure was 0.5%. Five dogs were examined using low‐field MRI. Lesions that were suspected to be intervertebral disc herniations were observed; however, there were no specific findings indicating that the nucleus pulposus had penetrated into the subarachnoid space or into the spinal cord parenchyma. Thus, the dogs were misdiagnosed as having a conventional intervertebral disc herniation. An intradural extramedullary disc herniation (three cases) or intramedullary disc herniation (two cases) was confirmed during surgery. By using computed tomographic myelography (CTM) for the remaining three dogs, an intradural extramedullary mass surrounded by an accumulation of contrast medium was observed and confirmed during surgery. Findings from this small sample of eight dogs indicated that CTM may be more sensitive for diagnosing canine thoracolumbar intradural disc herniation than low‐field MRI. 相似文献
74.
Yoshio Kiku Tomomi Ozawa Shiro Kushibiki Madoka Sudo Kouhei Kitazaki Noriaki Abe Hideyuki Takahashi Tomohito Hayashi 《Veterinary research communications》2010,34(2):197-203
During the postpartum period there is a high incidence of mastitis in dairy cows. The reason for this increased risk of mastitis
still remains unclear. Since leukocytes in colostrum have an important role in preventing the onset of mastitis, we investigated
the leukocyte populations, which express CD4, CD8, CD14, CD21 or WC1, in colostrum as well as in blood obtained from 14 Holstein
cows. Eight cows developed mastitis within a week after calving and the other 6 remained healthy. The percentage of CD14+
cells in colostrum was significantly lower in mastitic cows than in healthy cows. There were no significant differences in
other marker positive cells either in the colostrum or in the blood. The CD14+ cells in colostrum play an important role of
defense against invading microorganisms in the mammary glands. Our results suggested that the lower percentage of CD14+ cells
in colostrum might predict the incidence of mastitis in the following period. 相似文献
75.
76.
77.
Bending creep behavior of wood under cyclic moisture changes 总被引:1,自引:0,他引:1
Yaguang Zhou Masami Fushitani Takafumi Kubo Masayuki Ozawa 《Journal of Wood Science》1999,45(2):113-119
This study examined the bending creep behavior in the longitudinal direction of six species under cyclic moisture content (MC) changes. For each species, tests were made at 20°C with five cyclic relative humidity changes between 65% and 95%, beginning from moisture adsorption. A load corresponding to 25% of short-term breaking load of the species was applied to the radial section of each specimen with four-point bending. The effect of MC change on instantaneous compliance was also investigated under the same condition. The quantitative relation between mechano-sorptive (MS) compliance and MC change was examined, and the material parameter KM for the relation in specific sorption was determined. Results indicated that the total compliance in the six species with different behavior increases with sorption time. As an integral part of total compliance, instantaneous compliance changes linearly with MC and influences to a greater or lesser extent the total compliance behavior. In general, with increasing MC change, the MS compliance linearly increases during the first adsorption and all desorption and decreases slightly during subsequent adsorption. The material parameterK
M varies markedly not only with species but also with specific sorption. The first adsorption causes the largest deformation, followed by desorption.This paper was presented at the 48th annual meeting of the Japan Wood Research Society in Shizuoka, Japan. April 1998 相似文献
78.
Suyama Satoshi Ozawa Hitomi Shibata Yasutoki Fuji Taiki Nakagami Masayasu Shimizu Akio 《Fisheries Science》2019,85(3):495-507
Fisheries Science - We examine geographical differences in percentages of age-1 Pacific saury Cololabis saira with previous spawning experience collected from 143°E to 165°W during June... 相似文献
79.
Tropomyosins from fish skeletal muscle show high amino acid sequence homology, although their thermal stability is clearly
different among species. In order to determine the regions that are responsible for the stability of this protein, five synthetic
peptides of 30mer were synthesized by Fmoc method, based on the sequence of walleye pollack Theragra chalcogramma fast skeletal muscle tropomyosin, namely, N terminal Met1-Lys30, the variable region Asp84-Leu113, the middle region Val128-Ala157, the region containing the conservative Cys (Leu176-Lys205), and C terminal Asp255-Ile284. The thermal stability of these peptides was measured by circular dichroism and differential scanning calorimetry. The helical
contents of these peptides were decreased in a temperature-dependent manner, although they showed no clear melting temperature,
suggesting that the enthalpy necessary for the complete denaturation of these peptides was low. Peptides Asp255-Ile284 and Asp84-Leu113 showed the highest and second highest α-helical contents, respectively, and the other peptides gave rise to lower α-helical
contents. 相似文献
80.
Baba K Ishihara K Ozawa M Usui M Hiki M Tamura Y Asai T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(5):561-565
Antimicrobial administration is essential for the control and treatment of diseases in animals, but the emergence and prevalence of antimicrobial-resistant Staphylococcus aureus is a significant concern during animal production. Here we investigated the antimicrobial susceptibility of S. aureus from diseased food-producing animals and molecularly characterized the methicillin-resistant and fluoroquinolone-resistant isolates. A total of 290 S. aureus isolates obtained from cattle (n=246), swine (n=16), and chickens (n=28) between 2003 and 2009 were examined for antimicrobial susceptibility against 9 antimicrobials using an agar dilution method. Resistance to penicillin (PC) was most frequently found (24.8%), followed by oxytetracycline (OTC, 10.0%), dihydrostreptomycin (4.1%), erythromycin (EM, 3.1%), enrofloxacin (ERFX, 2.1%), and kanamycin (1.7%). The PC resistance rate was significantly higher in swine than in cattle (P<0.01) and chickens (P<0.01). The resistance rates to OTC, EM and ERFX were significantly higher in swine and chickens than in cattle (P<0.05). Methicillin-resistant S. aureus (MRSA) was recovered from milk derived from a cow with mastitis in 2003; sequence type 8, SCCmec type IV and spa type t024. In the six ERFX-resistant strains isolated after 2003, amino acid substitutions in ParC with/without GyrA were detected. As the prevalence of MRSA and FQ-resistant S. aureus in the animals should be noticed, continuous monitoring is necessary to control resistance to clinically important antimicrobials in S. aureus from food-producing animals. 相似文献