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31.
SV AA Henriksen R.J. Jørgensen P. Nansen KR Sejrsen J. Brolund Larsen S. Klausen 《Veterinary parasitology》1976,2(3):259-272
The investigation was designed to study the gastro-intestinal helminth infection established in yearling calves with reference to fluctuations in the larval contamination of the herbage during the grazing season. In a 2 × 3 factor experiment, comprising six groups, each of six calves, a comparison was made between infection levels, growth rates etc. in calves grazing the same paddock over an entire season and calves moved before the July rise in herbage contamination to paddocks not grazed earlier in the same season. A comparison was also made between animals receiving no anthelminthic treatment, animals treated 3 weeks after the start of the grazing season and again when moved, and animals treated every 3 weeks during the season.Before July there were no weight gain differences between the various groups of calves. During the rest of the season, the calves which were moved gained 647–869 g, while those that remained on the same paddock, and were exposed to a high level of larval contamination gained only 81–361 g per day. The effect of anthelmintic treatment was less pronounced, though significant. The weight gains were correlated to a high degree with the larval contamination levels of the respective paddocks (r = ? 0.79) as well as with the serum pepsinogen (r = ? 0.75) and albumin levels (r = 0.75) of the calves and to a minor degree also with faecal egg counts (r = ? 0.45). The observed weight gain differences could not be accounted for by differences in grass quantity or quality. 相似文献
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AE Pashitski A Gurevich AA Polyanskii DC Larbalestier A Goyal ED Specht DM Kroeger JA DeLuca JE Tkaczyk 《Science (New York, N.Y.)》1997,275(5298):367-369
Magneto-optical imaging was used to visualize the inhomogeneous penetration of magnetic flux into polycrystalline TlBa2Ca2Cu3Ox films with high critical current densities, to reconstruct the local two-dimensional supercurrent flow patterns and to correlate inhomogeneities in this flow with the local crystallographic misorientation. The films have almost perfect c-axis alignment and considerable local a- and b-axis texture because the grains tend to form colonies with only slightly misaligned a and b axes. Current flows freely over these low-angle grain boundaries but is strongly reduced at intermittent colony boundaries of high misorientation. The local (<10-micrometer scale) critical current density Jc varies widely, being up to 10 times as great as the transport Jc (scale of approximately 1 millimeter), which itself varies by a factor of about 5 in different sections of the film. The combined experiments show that the magnitude of the transport Jc is largely determined by a few high-angle boundaries. 相似文献
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Control of nematode parasites with reduced reliance on the use of anthelmintics was studied in 16 ewes with suckling twin lambs on contaminated pasture in Denmark. Ewes and lambs were treated with albendazole at turn-out 3 May. Ewes were removed from the groups on 26 July, and lambs were slaughtered on 11 October. The animals were allocated to 4 groups of 8 lambs and their 4 ewes. Group TS was treated with albendazole at weeks 3, 6 and 8 after turnout and set-stocked; group TM was similarly treated but moved to clean pasture in conjunction with the last drenching; group US was untreated and set-stocked, and group UM was left untreated but moved to clean pasture week 8 after turn-out. Supplementary feed was offered in June and August due to scarcity of pasture. Strategic treatments of ewes and lambs weeks 3, 6 and 8 after turn-out, with or without a move to clean pasture, were highly effective in controlling nematode infections for most of the season. This was reflected in better weight gains and carcass characteristics in the treated compared to untreated lambs, resulting in an average increase in the value of the product by 36%. The effect of moving without treatment (UM) on faecal egg counts was limited but peak pasture infectivity was reduced to less than 10% compared to the set-stocked group and weight gains of lambs were significantly better despite poor feed availability in late season. The study showed that under set-stocked conditions repeated anthelmintic treatments of both ewes and lambs in early season may ensure sufficient nematode control whereas moving animals to clean pasture without dosing was less efficient. The latter may, however, still be a viable option in organic and other production systems where routine use of anthelmintics is banned, particularly if weaning and moving are combined or a second move is performed. 相似文献
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Abstract AIM: To determine the effects of Cu and levamisole on concentrations of Cu and Fe in plasma and liver, and the effects of levamisole on lipid peroxidation induced by Cu intoxication in broiler chickens. METHODS: In a 2×4 factorial study, 80 one-day-old Ross PM3 broiler chicks were fed diets for 21 days containing either 8 mg/kg Cu (Low Cu) or 250 mg/kg Cu (High Cu) and were treated with 0 (L0), 4 (L4), 8 (L8) or 16 (L16) mg/kg bodyweight levamisole per day from Day 7 of the study, on three consecutive days in their drinking water. This treatment was repeated three times, at 3-day intervals. On Day 21, blood samples were collected from each bird for analysis of concentrations of Cu, Fe and malondialdehyde, and activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), superoxide dismutase, catalase and glutathione peroxidase (GSH-Px). The birds were killed and liver samples collected for analysis of Cu and Fe. RESULTS: Mean concentrations of Cu and Fe in plasma, and Cu in liver, were increased overall in the High Cu groups compared with the Low Cu groups (p<0.001). Compared with the L0 treatment group on the High Cu diet, treatments L4, L8 and L16 decreased concentrations of Cu in plasma, and L8 and L16 increased concentration of Cu in liver (p<0.05). Mean activities of AST and ALT were increased in untreated birds (L0) fed the High compared with Low Cu diets (p<0.01). In birds receiving the High Cu diet, treatments L4 and L8 decreased activities of AST, and L4 and L16 decreased activity of ALT, compared with L0 (p<0.05). The High Cu diet induced an oxidative stress characterised by increased mean concentrations of malondialdehyde and decreased activities of superoxide dismutase, catalase and GSH-Px (p<0.001). Concentration of malondialdehyde, and activities of superoxide dismutase and catalase were not changed following levamisole treatment in birds on the High Cu diet, and activity of GSH-Px was decreased by the L4 and L8 treatments compared with the L0 group. CONCLUSIONS AND CLINICAL RELEVANCE: The results of the study suggest that treatment with levamisole might alleviate the harmful effects of Cu on the liver, as demonstrated by decreased activities of AST and ALT induced by a diet containing 250 mg/kg Cu. 相似文献
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Summary Infectious bursal disease virus antigen was detected in the bursa of Fabricius of infected birds by the use of the counter
immunoelectroosmophoresis technique. Eight suspected outbreaks were confirmed in this way and 82 out of 89 bursal samples
submitted for laboratory confirmation were positive. The test was also suitable for the detection of antibody to infectious
bursal disease virus in sera of infected birds. Precipitin lines were visible within 30 min as compared with 18 to 24 h with
the Ouchterlony agar gel precipitation test. Counter immunoelectroosmophoresis is recommended for rapid confirmation of infectious
bursal disease of poultry.
Resumen Mediante el empléo de la técnica de contra inmunoelectroosmoforesis, se detectó antigeno viral en la bolsa de Fabricio de aves infectadas con Gumboro. Se confirmaron ocho brotes sospechosos mediante la técnica, y 82 de 89 muestras tomadas de bolsas sospechosas y analizadas en el laboratorio fueron positivas. La prueba tambien fue útil para la detección de anticuerpos de Gumboro en el suero de animales infectados. Las lineas de precipitación fueron visibles a los 30 minutos en comparación a las 18 a 24 horas necesarias para leer la prueba de Ouchterlony. Se recomienda la contra inmunoelectroosmoforesis para el diagnóstico de la enfermedad de Gumboro en aves.
Résumé L'antigène du virus de la bursite infectieuse aviaire a été détecté dans les bourses de Fabricius d'oiseaux infectés en utilisant la technique de contre immuno-électro-osmo-phorèse. Huit foyers suspects ont été confirmés dans cette manière et, sur 89 prèlevements de bourses soumis pour confirmation de diagnostic au laboratoire, 82 ont été trouvés positifs. Le test est indiqué pour la détection d'anticorps anti-virus de la bursite infectieuse aviaire dans les sérums d'oiseaux infectés. Des lignes de précipitation sont visibles dans les trente minutes alors qu'elles n'apparaissent qu'en 18–24 heures avec le test d'Ouchterlony en gélose. La contre immuno-électro-osmo-phorèse est recommandée pour une confirmation rapide de la bursite infectieuse aviaire.相似文献