Identification and characterization of a novel lymphocystis disease virus isolate from cultured grouper in China

Grouper Epinephelus spp. is one of the most important mariculture fish species in China and South-East Asian countries. The emerging viral diseases, evoked by iridovirus which belongs to genus Megalocytivirus and Ranavirus, have been well characterized in recent years. To date, few data on lymphocystis disease in grouper which caused by lymphocystis disease virus (LCDV) were described. Here, a novel LCDV isolate was identified and characterized. Based on the sequence of LCDV major capsid protein (MCP) and DNA polymerase gene, we found that the causative agents from different species of diseased groupers were the same one and herein were uniformly defined as grouper LCDV (GLCDV). Furthermore, H&E staining revealed that the nodules on the skin were composed of giant cells that contained inclusion bodies in the cytoplasm. Numerous virus particles with >210 nm in diameter and with hexagonal profiles were observed in the cytoplasm. In addition, phylogenetic analysis based on four iridovirus core genes, MCP, DNA polymerase, myristoylated membrane protein (MMP) and ribonucleotide reductase (RNR), consistently showed that GLCDV was mostly related to LCDV-C, followed by LCDV-1. Taken together, our data firstly provided the molecular evidence that GLCDV was a novel emerging iridovirus pathogen in grouper culture.     

叶面肥对无土栽培樱桃番茄性状的影响及产量与植株性状的数学模型研究

为了探索樱桃番茄无土栽培在海南热带地区种植效果及植株产量效应,本研究通过增施不同量叶面肥,设计3种处理、3次重复随机试验,并对试验最佳效果的植株产量、株高和茎粗建立相关数学模型。结果表明,增施叶面肥较传统施肥有明显增产效果,平均增产2250 kg/hm²,增产20.2%。而樱桃番茄株高与产量、茎粗与产量具有显著相关性,其模型分别为Y_单株产量=(47.92060X_{株高-24.95763)² (P= 0.0114＜0.05)和Y单株产量=(61.885X茎粗-46.97988)² (P=0.0372＜0.05),符合植株生长趋势变化。因此,在海南开展樱桃番茄无土栽培是可行的,且可以通过调控来调节植株生长情况从而提高产量,增加经济效益。}     

Nitrous oxide emissions from an apple orchard soil in the semiarid Loess Plateau of China

Nitrous oxide (N₂O) fluxes from an apple orchard soil in the semiarid Loess Plateau of China were measured using static chambers from September 2007 to September 2008. In this study, three sites were selected at distance of 2.5 m (D 2.5), 1.5 m (D 1.5), and 0.5 m (D 0.5) from the apple tree row. Nitrous oxide fluxes followed seasonal pattern, with high N₂O emission rates occurring in the hot-humid summer and low rates in the cold-dry winter. Pulses of N₂O emissions occurred after nitrogen fertilizer application, summer rainfall events, and during freeze-thaw cycles. Annual average N₂O emission rates were the highest at D 0.5 site (48.2 ± 39.9 μg N₂O m⁻² h⁻¹), the lowest at D 2.5 site (31.9 ± 18.2 μg N₂O m⁻² h⁻¹), and intermediate at D1.5 site (36.8 ± 32.2 μg N₂O m⁻² h⁻¹), suggesting that N₂O emissions from the apple orchard soil increased when the chamber location was closer to the apple tree row. This may be due to the fertilization close to roots in hot and humid season. Over one third (37.1%) of the annual N₂O emission occurred in the summer. Annual N₂O emissions from the apple orchard soil averaged to 3.22 kg N₂O ha⁻¹ year⁻¹. Annual emission factor of the apple orchard from the applied fertilizer (uncorrected for background emission) was 0.658%. This value was nearly a half (53%) of the default value provided by the Intergovernmental Panel on Climate Change for the application of synthetic fertilizers to cropland (1.25%). Therefore, the amount of N₂O emissions from the semiarid apple orchard soil could be largely overestimated if no regional-specific factor is used.     

Influence of Common Reed (Phragmites australis) on CH4 Production and Transport in Wetlands: Results from Single-Plant Laboratory Experiments

Laboratory culture experiments were conducted with common reed (Phragmites australis) to elucidate the role of root exudates on CH₄ production in wetland soils as well as the importance of different plant organs as routes of CH₄ to the atmosphere. In the 50 d experiment period, root exudates ranged from 0.03 to 1.53 µmolg^?1d^?1, which increased with reed growth. CH₄ production rate of soil was stimulated as root exudates collected was added. CH₄ transport capacity rate also increased with plant growth and influenced by light intensity. Root tips were the most important part of controlling diffusion of CH₄ into reed shoots, and leave transport accounted for 45.34% of total emissions into the atmosphere.     

Application of Response Surface Methodology to Analyze the Effects of Soil/Liquid Ratio,pH, and Incubation Time on the Bioaccessibility of PAHs from Soil in In Vitro Method

Water, Air, & Soil Pollution - Central composite design using response surface methodology was employed to optimize soil/liquid ratio (S/L), pH, and incubation time for polycyclic aromatic...     

A STELLA model for the estimation of atrazine runoff,leaching, adsorption,and degradation from an agricultural land

Background, aim, and scope  

Atrazine (2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine) is one of the most widely used herbicides for broadleaf weed and certain annual grass controls and is popular because of its effectiveness and low cost. Losses of atrazine from agricultural lands into adjacent surface water and underlying groundwater have raised public concerns. Several computer models have been developed for atrazine runoff in watersheds. One limitation for most of these models, however, is that vast amounts of input parameters are required for simulations. These input parameters are sometimes difficult to obtain through the experimental measurements for model calibrations, validations, and applications. Therefore, a need exists to develop a simple and yet a realistic modeling tool that can be used for effectively investigating atrazine dynamics in agricultural soils. This study was designed to meet this need.     

绿色农药分散剂GCL4-1的结构特征及应用性能研究

采用红外光谱、电位滴定、凝胶渗透色谱对改性木质素磺酸钠农药分散剂GCL4-1的分子结构进行了研究,结果表明,GCL4-1分子中磺酸基含量较改性前的木质素磺酸钠LS有明显提高,磺化度从改性前的1.33 mmol/g增加到1.96 mmol/g;其重均分子量为9700,数均分子量为2300。进一步研究了GCL4-1对农药水分散粒剂(WG)应用性能和药效等的影响,发现以GCL4-1作分散剂制备的WG具有良好的润湿性和崩解性,WG热贮前后的悬浮率都超过90%;WG悬浮液分散稳定性优良,2 h后的悬浮液沉淀层厚度仅为0.386 mm,颗粒粒径为7.22 μm。制得的80%烯酰吗啉WG防治黄瓜霜霉病的EC90值为58.32 mg/L,80%氟虫腈WG样品对小菜蛾的LC50值为0.5196 mg/L,室内毒力达到了国外同类制剂最好产品水平。GCL4-1的综合性能达到甚至超过Borregaard公司的Kinsperse126,是一种具有优良性价比的绿色农药分散剂。     

颗粒状秸秆物料流动特性试验

以华北地区8种农作物秸秆为研究对象,通过颗粒状秸秆静态堆积角、压缩率、均匀度、抹刀角4个指标测试,采用Carr指数法及内摩擦角评价分析了各种颗粒状秸秆物料的流动特性。结果表明,通过Carr指数法得分可以反映颗粒状秸秆流动性的变化规律,得分越高,流动性越好;8种秸秆分为易于流动(如玉米芯)、一般流动(如玉米秸秆)、不易流动(如豆秸)3类,该结果与采用内摩擦角评价原料的流动特性基本一致。     

施肥和密度对苎麻营养生理的影响

为了探讨施肥和密度对苎麻产量和品质形成的综合影响及营养生理效应,以苎麻新品种0501为试验材料,采用二次正交旋转组合设计,研究了施肥和密度对苎麻养分积累、苎麻养分分配规律的影响。结果表明,氮肥对苎麻植株体内的氮磷钾含量影响显著,施氮促进了苎麻对氮素的积累,但是,增施氮肥不利于磷钾在苎麻体内的积累。钾肥可以显著促进麻皮对磷钾的积累,使磷钾向皮中转移。养分分配规律中,头麻与二麻在养分分配中略有差别。     

人乳腺癌细胞、结肠癌细胞和宫颈癌细胞生长曲线测定

为检测比较人乳腺细胞(MDA-MB-231、MCF-7、HBL-100)、人结肠癌细胞(HCT-8)和人宫颈癌细胞(HeLa)生长曲线的差异性.本实验首先常规培养MDA-MB-231、MCF-7、HBL-100、HCT-8和HeLa细胞,然后应用MTT方法测定这几种细胞的生长曲线.在本实验条件下,这几种细胞生长速度从高...