全文获取类型
收费全文 | 584篇 |
免费 | 13篇 |
国内免费 | 3篇 |
专业分类
林业 | 26篇 |
农学 | 9篇 |
基础科学 | 1篇 |
86篇 | |
综合类 | 20篇 |
农作物 | 12篇 |
水产渔业 | 108篇 |
畜牧兽医 | 305篇 |
植物保护 | 33篇 |
出版年
2023年 | 3篇 |
2022年 | 18篇 |
2021年 | 37篇 |
2020年 | 10篇 |
2019年 | 3篇 |
2018年 | 11篇 |
2017年 | 6篇 |
2016年 | 13篇 |
2015年 | 30篇 |
2014年 | 34篇 |
2013年 | 54篇 |
2012年 | 33篇 |
2011年 | 33篇 |
2010年 | 25篇 |
2009年 | 14篇 |
2008年 | 35篇 |
2007年 | 36篇 |
2006年 | 34篇 |
2005年 | 35篇 |
2004年 | 31篇 |
2003年 | 31篇 |
2002年 | 28篇 |
2001年 | 18篇 |
2000年 | 9篇 |
1999年 | 4篇 |
1998年 | 2篇 |
1997年 | 2篇 |
1996年 | 4篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1993年 | 2篇 |
1988年 | 1篇 |
1980年 | 1篇 |
1953年 | 1篇 |
排序方式: 共有600条查询结果,搜索用时 15 毫秒
121.
122.
Natsuki KASUYA Qin XU Yoshinao KOBAYASHI Kiyoharu FUKUDA Osamu ENISHI Kenji IIYAMA Hisao ITABASHI 《Animal Science Journal》2008,79(2):200-209
Four tropical and four temperate grasses were subjected to nylon bag fiber degradation study with fistulated Holstein cattle as well as in vitro digestion with broad spectrum cellulase, and the degradability of cell wall carbohydrate and lignin for the respective forage grasses were determined. The degradability of neutral detergent fiber and acid detergent fiber for the same plant materials appeared to be similar, but that of acid detergent lignin was constantly lower. As for the plant materials used in the present study, Italian ryegrass, orchardgrass and guineagrass showed high degradability of fibers in nylon bag incubation, while setaria, cogongrass and wheat showed smaller degradability. In both nylon bag incubation and in vitro digestion experiments, the forage samples showing relatively lower degradability exhibited notably lower disappearance of xylose than that of glucose or arabinose. It was also observed that dry matter degradability correlated negatively with the initial content of esterified and/or etherified p‐coumaric acid. These results were assumed to be caused by the difference in resistance towards rumen microbial degradation due to the concentration of lignin‐carbohydrate complex. 相似文献
123.
Akira YABUKI Keijiro MIZUKAMI Satoshi TOKUNAGA Osamu YAMATO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(1):109-112
Ghrelin is a peptide hormone that is mainly produced by the stomach. The kidney is a
major source of local ghrelin, and maintaining body fluid balance is considered a critical
role of renal ghrelin. However, there are no reports on renal ghrelin in small animal
medicine. The present study investigated the intrarenal localization of and change in
ghrelin expression in dogs with immune complex-mediated glomerulonephritis (ICGN). Ghrelin
immunoreactivity (IR) was observed in the distal tubules of normal kidneys. Ghrelin IR was
weak in ICGN kidneys, and the quantitative ghrelin IR score was significantly lower in
ICGN kidneys than in normal kidneys. In cases of ICGN, plasma creatinine concentrations
showed a positive correlation with the ghrelin IR score. 相似文献
124.
Pollen-mediated transgene flow is a major concern for the production of genetically modified (GM) rice. Cleistogamy is a useful tool for preventing this form of gene flow. We previously identified the cleistogamous rice mutant superwoman1-cleistogamy (spw1-cls) and determined its molecular genetic mechanism. In the present study, we cultivated spw1-cls over five years to examine effects of cleistogamy on agronomic traits. Simultaneously, we cultivated cleistogamous backcross lines created by continuous backcrossing with “Yumeaoba” (a japonica cultivar) as the recurrent parent and by application of a DNA marker. In these experimental cultivations, spw1-cls and its backcross lines showed almost equal or slightly lower, but acceptable, agronomic traits compared with each control line. We also conducted natural crossing tests in paddy fields to assess the gene containment capability of spw1-cls. In a series of field experiments, there was no natural crossing between spw1-cls (pollen donor) and pollen recipient lines, but the wild-type donor and recipient lines were crossed. Thus, the cleistogamy of the spw1-cls mutation is able to inhibit natural crossing effectively, without significant loss of commercial benefits, such as yield. We conclude that spw1-cls cleistogamy is a practical tool for gene containment in GM rice cultivation. 相似文献
125.
Maho NAKAZAWA Hirotaka TOMIYASU Kanako SUZUKI Hajime ASADA Aki FUJIWARA-IGARASHI Yuko GOTO-KOSHINO Aki OHMI Koichi OHNO Michio FUJITA Hajime TSUJIMOTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(3):456
Nasal lymphoma (NL) is the most common nasal tumor in cats, and radiotherapy, chemotherapy, or a combination of these treatments have been described as the treatment for this disease. However, the previous studies included various machines and protocols of radiotherapy. Therefore, we aimed to retrospectively compare the prognosis among cases treated with palliative hypofractionated radiotherapy, chemotherapy, and a combination of them with united machine and protocol of radiotherapy. When compared overall survival and progression free survival, there was no significant difference among these three groups. The data of this study suggested that similar efficacy could be achieved by palliative hypofractionated radiotherapy, chemotherapy, or a combination of them. 相似文献
126.
Aritada YOSHIMURA Takahiro OHMORI Kokoro ITOU Ryo ISHI Yuri MATSUMURA Yuhei WADA Miori KISHIMOTO Tomoko IWANAGA Naoki MIURA Kazuhiko SUZUKI Ryuji FUKUSHIMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(5):780
In dogs, pancreatic acinar cell injury is thought to be caused by decreased pancreatic blood flow due to heart failure. In previous our report, it demonstrated that decreased heart function causes a significant decrease in pancreatic blood flow in heart failure dog model caused by rapid ventricular pacing (RVP). However, the types of histopathological changes remain unclear. We aimed to verify the types of histopathological changes occurring in the pancreatic tissue due to decreased heart function. After RVP for 4 weeks, atrophy of pancreatic acinar cells, characterized by a decrease in zymogen granules, was observed in all areas of the pancreas. In conclusion, the result of this study suggests that attention should be paid to ischemia/hypoperfusion injury in the pancreas. 相似文献
127.
Kensaku Maejima Misako Himeno Osamu Netsu Kazuya Ishikawa Tetsuya Yoshida Naoko Fujita Masayoshi Hashimoto Ken Komatsu Yasuyuki Yamaji Shigetou Namba 《Journal of General Plant Pathology》2014,80(2):176-183
Sharka disease, caused by plum pox virus (PPV), is the most serious viral disease of stone fruit trees. Among the eight known strains of the virus, PPV-D is the most important due to its recent global spread. Although enzyme-linked immunosorbent assay (ELISA) is the most common approach for diagnosing sharka, it involves time-consuming steps and requires expensive equipment and trained technicians. In this study, an on-site PPV detection kit based on immunochromatography was developed using polyclonal antibodies against the coat protein (CP) of a PPV-D isolate. The immunochromatographic (IC) assay kit was as sensitive as a commercial ELISA system for detecting Japanese PPV-D isolates. Moreover, it was easy to use (a one-step procedure), and results could be obtained on-site within 15 min without special laboratory equipment. The IC assay kit detected the virus from every aerial part of symptomatic Japanese apricot trees. In a detailed study of viral localization in leaves, the most suitable plant parts for use in the IC assay were symptomatic mesophyll tissues and the region from the petiole to the main vein. A positive reaction was also observed using the CP of other major (PPV-M and PPV-Rec) and minor (PPV-EA, PPV-W, and PPV-T) strains. 相似文献
128.
Taira O Watanugi I Hagiwara Y Takahashi M Arai S Sato H Maehara N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(10):1059-1062
We cloned five new subtypes of cDNA encoding canine interferon-alpha (CaIFN-alpha) from a canine epithelial cell line. CaIFN-alphas were divided into two groups by amino acid sequences and a molecular phylogenic tree. Two subtypes of them were expressed in Escherichia coli, and IFN proteins were purified. Recombinant CaIFN-alphas were highly species-specific and showed antiviral activity against Vesicular stomatitis New Jersey virus and canine adenovirus-1 , but not against canine herpesvirus-1. 相似文献
129.
Shogo HIGAKI Hongyu DARHAN Chie SUZUKI Tomoko SUDA Reina SAKURAI Koji YOSHIOKA 《The Journal of reproduction and development》2021,67(1):67
We aimed to determine the effectiveness of estrus detection based on continuous measurements of the ventral tail base surface temperature (ST) with supervised machine learning in cattle. ST data were obtained through 51 estrus cycles on 11 female cattle (six Holsteins and five Japanese Blacks) using the tail-attached sensor. Three estrus detection models were constructed with the training data (n = 17) using machine learning techniques (random forest, artificial neural network, and support vector machine) based on 13 features extracted from sensing data (indicative of estrus-associated ST changes). Estrus detection abilities of the three models on test data (n = 34) were not statistically different among models in terms of sensitivity and precision (range 50.0% to 58.8% and 60.6% to 73.1%, respectively). The relatively poor performance of the models might indicate the difficulty of separating estrus-associated ST changes from estrus-independent fluctuations in ST. 相似文献
130.
Nopporn Manajit Takafumi Arimoto Osamu Baba Seiichi Takeda Aussanee Munprasit Kamolrat Phuttharaksa 《Fisheries Science》2011,77(4):447-454
The Japanese type of set-net, Otoshi-ami, was introduced to Thailand in 2003 with the aim of assessing its feasibility as
a sustainable coastal fisheries management tool for the empowerment of coastal fishers’ communities. All preparations for
constructing and installing the set-net in the coastal waters off Mae Rumpheung beach, Rayong Province, Thailand, were carried
out by local fishers, with technical advice and support from national and international institutions. The gear was modified
and developed to suit the conditions of the fishing ground and target species. Data on the catch and sales trends in Rayong
using the set-net were collected for 7 years for statistical analysis; during this period, the gear design and marketing strategies
were improved. Simulation analysis for evaluating the cost–profit bases was conducted to establish a model for set-net technology
transfer in Southeast Asia, based on differing numbers of fishers and operation days. The simulation results show the required
size of the average daily catch to cover the total cost according to the average unit price, where the economic return point
is an average catch of 128 kg, based on a unit price of 25 Baht/kg (0.83 USD/kg), with ten fishermen and a daily operation
cost of 3,200 Baht (USD 106.17). 相似文献