Glacial survival of boreal trees in northern Scandinavia

It is commonly believed that trees were absent in Scandinavia during the last glaciation and first recolonized the Scandinavian Peninsula with the retreat of its ice sheet some 9000 years ago. Here, we show the presence of a rare mitochondrial DNA haplotype of spruce that appears unique to Scandinavia and with its highest frequency to the west-an area believed to sustain ice-free refugia during most of the last ice age. We further show the survival of DNA from this haplotype in lake sediments and pollen of Tr?ndelag in central Norway dating back ~10,300 years and chloroplast DNA of pine and spruce in lake sediments adjacent to the ice-free And?ya refugium in northwestern Norway as early as ~22,000 and 17,700 years ago, respectively. Our findings imply that conifer trees survived in ice-free refugia of Scandinavia during the last glaciation, challenging current views on survival and spread of trees as a response to climate changes.     

Mach-Zehnder interferometry in a strongly driven superconducting qubit

We demonstrate Mach-Zehnder-type interferometry in a superconducting flux qubit. The qubit is a tunable artificial atom, the ground and excited states of which exhibit an avoided crossing. Strongly driving the qubit with harmonic excitation sweeps it through the avoided crossing two times per period. Because the induced Landau-Zener transitions act as coherent beamsplitters, the accumulated phase between transitions, which varies with microwave amplitude, results in quantum interference fringes for n = 1 to 20 photon transitions. The generalization of optical Mach-Zehnder interferometry, performed in qubit phase space, provides an alternative means to manipulate and characterize the qubit in the strongly driven regime.     

Coral communities of Biscayne Bay,Florida and adjacent offshore areas: diversity,abundance, distribution,and environmental correlates

• 1. Hardbottom habitats of Biscayne Bay, a shallow lagoon adjacent to the city of Miami, Florida, USA, contain a limited number of coral species that represent a small subset of the species found at nearby offshore hardbottom and reef habitats of the Florida Reef Tract. Although the physical characteristics of this basin make it a marginal environment for coral growth, the presence of dense populations of Siderastrea radians and Porites furcata indicate that these, as well as other corals that are found at lower densities, are able to tolerate extreme and fluctuating conditions. Three factors, temperature, sedimentation, and salinity, appear to limit coral abundance, diversity, and distribution within Biscayne Bay.
• 2. Temperatures exhibit high frequencies of extreme high and low values known to cause coral stress and mortality elsewhere. Similarly, sedimentation rates are very high and sediment resuspension caused by currents, storms and boating activities commonly bury corals under sediment layers. Sediment burial was shown experimentally to influence growth and mortality of S. radians.
• 3. The salinity of Biscayne Bay is influenced by freshwater inputs from canal, sheetflow and groundwater sources that create a near‐shore environment with low mean salinity and high salinity fluctuation. Coral communities along this western margin have the lowest coral density and species richness. Chronic exposure to low salinity was shown experimentally to cause a decrease in the growth of S. radians.
• 4. The location of Biscayne Bay, downstream of a large restoration effort planned for the Everglades watershed, highlights the need to understand the relationship between the physical environment and the health of benthic communities. The data presented here provide the type of scientific information needed so that management decisions can take into account the potential impacts of human activities on the health of coral populations that are already near their tolerance limits for temperature, salinity, and sedimentation.

Copyright © 2003 John Wiley & Sons, Ltd.     

Congenital diffuse hyperplastic goiter associated with perinatal mortality in 11 captive-born bottlenose dolphins (Tursiops truncatus).

Diffuse hyperplastic goiter was diagnosed by histopathology in 11 perinatal bottlenose dolphins (Tursiops truncatus) that died at four separate zoos and aquaria. Thyroid morphology of these animals was compared with the histologically normal thyroids of two stranded wild bottlenose dolphin calves, a neonate and a 2-mo-old calf. Histologic changes included reduced follicular luminal diameter, markedly reduced or absent luminal colloid, hypertrophy of follicular epithelium, and follicular dysplasia. The etiology of the thyroid gland lesion was not identified. Cause of death was not determined for most of these animals, but they were presumed to have died from metabolic derangements associated with the thyroid lesion, drowning, or dystocia.     

Comparison of spawning induction techniques on <Emphasis Type="Italic">Paracentrotus lividus</Emphasis> (Echinodermata: Echinoidea) broodstock

The performance of different and expedite spawning induction methods was compared on samples of both mature wild and captive Paracentrotus lividus sea urchin populations. Thermal, saline and mechanical shocks, emersion during different periods of time, addition of con-specific gametes and different KCl concentrations were tested. Percentage of spawned sea urchins, mean number of released eggs, percentage of males that released more than 200 million spermatozoa, and survival after 5 days were the variables analysed. Results indicate that both thermal and saline shocks were ineffective methods to trigger spawning. Mechanical shock and addition of con-specific gametes were able to promote spawning but with a reduced number of released gametes. Emersion for a period of 3 h induced spawning with 100% broodstock survival but longer periods up to 12 h, although increasing the number of spawners can cause significant broodstock mortality. An injection of 1-mL intra-peristomial KCl was an expedite method to obtain P. lividus gametes, but high mortalities were always recorded and was related with excessive KCl concentrations. When there is need for a small number of gametes, the mechanical shock technique can be considered since led to 100% survival. When large spawnings are required, the emersion procedure can be a viable method, but further investigation must be carried out to assess the best time period to obtain broodstock full survival.     

Prevalence and Genotypic Characterization of Clostridium difficile From Ruminants in Switzerland

Clostridium difficile is an emerging enteric pathogen of humans and animals with a known main reservoir in the intestinal tract of various warm‐blooded animals. This study was carried out to evaluate the prevalence of C. difficile in 150 rectal swab samples collected from cattle and goats in Switzerland. The overall prevalence of C. difficile was 6.6%. The isolates belonged to the PCR ribotypes 033, 066, 070, 003, 001 and 137. In addition, the occurrence of C. difficile in faecal samples collected from farm ground was also evaluated and C. difficile was detected in 21% (7/30) of these samples. These isolates belonged to ribotypes 033, 066, 014 and 137. Several isolates pertained to ribotypes known to cause Clostridium difficile infections in humans.