Reliability Evaluation of Sampling Plan Fixed by Council Directive 91/68/EEC for the Maintenance of Officially Brucellosis‐free Flock Status

The European Union (EU) strategy with respect to sheep and goat brucellosis aims to eradicate the infection and achieve officially brucellosis‐free (OBF) status in all EU holdings and territories. Council Directive 91/68/EEC of 28 January 1991 states that to maintain OBF status of ovine or caprine holdings located outside an OBF territory, only a representative number of animals need to be tested annually. However, depending on the number of animals in a holding, this testing method risks non‐detection of the infection, thereby reducing the efficacy of the brucellosis control plan. The recommended sampling procedure has a low sensitivity for detecting infection in medium‐sized flocks; furthermore, the risk of not detecting re‐infection in OBF flocks, particularly in territories that have not yet gained OBF status, is also not acceptable. Moreover, in large‐sized flocks, the Directive sampling procedure entails taking an excessive number of samples, which can be very expensive. The authors evaluated, by using statistical analyses and a simulation model based on field data, the possible consequences of the current EU strategy. It is suggested that the sampling criteria for the maintenance of OBF status in the EU should be modified and that a statistically based sampling method should be applied instead of the fixed percentage method that is currently in use.     

Estimation of preliminary reference values for macro and micronutrients in ‘Valencia’ orange in Venezuela,by means of the compositional nutrient diagnosis system

The present study was aimed at developing preliminary reference values for foliar tissue for nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), copper (Cu), iron (Fe), manganese (Mn), and zinc (Zn) for `Valencia´ orange in Venezuela, using the Compositional Nutrient Diagnosis system (CND). References values were derived from a database comprising the nutrients concentrations and the yields of `Valencia´ orange trees (Citrus sinensis L. Osbeck) grafted on two different rootstocks, viz., Volkamer lemon (Citrus volkameriana Pasq.), and Cleopatra tangerine (Citrus reshni, Hort. ex. Tan). Cutoff values separating high- and low-yield sub-samples were estimated through modeling the cumulative variance ratio function versus yield relationships with the Boltzmann equation. For macronutrients, the norms developed were dependent upon the inclusion of the micronutrients concentrations in the calculations, and on the rootstocks. Care must thus be exercised, since nutrient indexes, and thus nutritional diagnosis, are based upon proper selection of such values.     

Characterization, In Vitro Culture, and Molecular Analysis of Thecaphora solani, the Causal Agent of Potato Smut

ABSTRACT The fungus Thecaphora solani (syn.: Angiosorus solani), the causal agent of potato smut, was cultivated in vitro for the first time. Teliospores obtained from galls of infected potato plants were used to inoculate commonly used solid and liquid media. The teliospores produced two kinds of vegetative tissue depending on the nutrient status of the media. A very slow radial-growing, hyaline, and septate mycelium, as usually seen in most of the in vitro-cultivated filamentous fungi, was obtained in wateragar medium after 30 to 40 days. On the other hand, a white, sponge-like mycelial mass was obtained in HCM + 1% activated charcoal, and on common potato dextrose agar or malt-yeast-peptone solid or liquid media, after 40 to 50 days under lab conditions. The identity among teliospores and the sponge-like mycelial mass was corroborated by DNA fingerprinting and partial sequencing of the large subunit (LSU) rDNA region. The sexual cycle of the pathogen was completed under lab conditions based on the development of teliospores on the sponge-like mycelial mass. The first attempt to reproduce the disease under controlled conditions was successful, inducing a gall in a cv. Desirée potato explant cultivated in vitro inoculated with radial-growing mycelia. Phylogenetic analysis of LSU rDNA data of the genus Thecaphora and other smut fungi confirmed the initial classification of the pathogen as T. solani.