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41.
Ultrastructural alteration of anionic sites (ASs) in the glomerular basement membrane (GBM) was studied in glomerulonephritis characterized by linear capillary IgG deposition in four dogs infected with Dirofilaria immitis and two normal control dogs using polyethyleneimine. ASs were identified as small dense particles distributed regularly in the lamina rara externa (LRE), but there were no ASs in the lamina densa (LD) of the GBM of the control dogs. In the glomeruli of the infected dogs, ASs were distributed regularly or irregularly in the thickened LD. ASs were in addition localized over the characteristic continuous bands of subendothelial dense deposits. The number of ASs of the LRE increased in all four infected cases as compared to the controls (p<0.01).  相似文献   
42.
The thermodynamic properties of myosin and its C-terminal fragment, light meromyosin (LMM), from walleye pollack, a typical cold-water fish efficiently utilized on an industrial scale, were analyzed by using differential scanning calorimetry (DSC) and circular dichroism (CD) spectrometry. Recombinant walleye pollack LMM expressed in Escherichia coli was also subjected to DSC and CD measurements for reference. The two proteins prepared from frozen surimi showed three endothermic peaks, the transition temperatures (T(m)) of which were quite similar, although overall DSC patterns differed considerably from one another. Their alpha-helical contents determined by CD were low compared to values reported before for other species. On the other hand, recombinant LMM gave four endothermic peaks at 27.4, 30.8, 36.5, and 43.4 degrees C in DSC and showed an alpha-helical content of approximately 80%. The peak at 27.4 degrees C could not be observed in walleye pollack LMM prepared from frozen surimi and thus was possibly attributed to its C terminus, because this extreme C-terminal region is supposedly truncated during preparation of LMM by tryptic digestion.  相似文献   
43.
Seaweeds are valuable sources of biologically active compounds that could be used as ingredients for pharmacological applications. Industrial fermentation using microorganisms provides a wide array of fermented foods and functional compounds with excellent health benefits. These fermentation-derived natural compounds have the potential to be applied as nutraceuticals and functional foods. Therefore, this paper presents an overview of the fermentation of sustainable seaweeds in producing valuable components, including anticoagulant, antioxidant, antimicrobial, anti-inflammatory, and anticancer. Fermentation-derived compounds are expected to receive more attention due to their environmentally friendly processing as well as consumer desire for natural foods.  相似文献   
44.
针对固定式图像采集设备终端在实时采集过程中存在的采集角度和范围有限等问题,研究开发了一种固定桁架式地面移动多点作物图像远程获取系统。系统采用网络摄像机和电动变焦镜头作为图像摄取设备,通过全方位智能控制云台、螺旋四杆机构和水平滑块导轨机构的设计与选型,建立了作物图像摄取设备的水平移动、垂直移动、360°空间转动、俯仰运动等子系统。开发了远程控制软件,建立了作物图像数据库和远程管理系统,实现了监测区域内所有植株不同部位图像信息的远程控制获取和管理。系统在温室中进行了运行测试,运行正常可靠。该系统可为作物远程在线水肥诊断提供了一种空间移动多点实时监测和远程管理平台。  相似文献   
45.
The effect of different component of the intestinal fluid on the excystation of Haplorchis taichui metacercariae was examined in vitro. Encysted metacercariae obtained by pepsin digestion of fish muscle showed that the highest percentage of metacercariae excystation could be obtained after being treated with trypsin at pH 7.5. However, it was also observed that excystation of the metacercariae could also be effected by changing the pH to the basic condition. The most optimum pH for the metacercariae excystation was found to be 7.5. This method of obtaining the excysted metacercariae could prove to be useful for taxonomical study of trematode in general.  相似文献   
46.
The marine sponge Haliclona simulans collected from the Irish Sea yielded two new steroids: 24-vinyl-cholest-9-ene-3β,24-diol and 20-methyl-pregn-6-en-3β-ol,5α,8α-epidioxy, along with the widely distributed 24-methylenecholesterol. One of the steroids possesses an unusually short hydrocarbon side chain. The structures were elucidated using nuclear magnetic resonance spectroscopy and confirmed using electron impact- and high resolution electrospray-mass spectrometry. All three steroids possess antitrypanosomal and anti-mycobacterial activity. All the steroids were found to possess low cytotoxicity against Hs27 which was above their detected antitrypanosomal potent concentrations.  相似文献   
47.
48.
We investigated the thermal denaturation of carp F-actin by measuring the loss of polymerization ability. The thermal denaturation rates of carp F-actin were at least 10-fold higher than those of chicken F-actin. Binding of tropomyosin thermally stabilized carp F-actin with no appreciable change in activation energy, suggesting the instability was caused by a high frequency of fragmentation of the actin filaments. A comparison of the critical concentration for polymerization suggested that the subunit–subunit interactions of carp F-actin were indeed weaker than those of chicken F-actin. Furthermore, using fluorescence quenching we showed that the nucleotide binding region of carp F-actin was in a more open conformation. Together, our results suggest that the instability of carp F-actin is a function of both the rate of fragmentation and the dissociation of bound nucleotides.  相似文献   
49.
50.
Over a period of roughly 40 days, starting on 8 July 2000, a caldera structure 1.7 kilometers in diameter developed by means of gradual depression and expansion of the summit crater at Miyake Island, Japan. At the same time, very-long-period (VLP) seismic signals were observed once or twice a day. Source mechanism analyses of the VLP signals show that the moment tensor solutions are smooth step functions over a time scale of 50 seconds, with dominant volumetric change components. We developed a model to explain the caldera and the VLP signals, in which a vertical piston of solid materials in the conduit is intermittently sucked into the magma chamber by lateral magma outflow. This model offers potential for making quantitative estimations of the characteristic physical properties of magma systems.  相似文献   
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