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71.
Takuma AOKI Hiroshi SUNAHARA Keisuke SUGIMOTO Tetsuro ITO Eiichi KANAI Sakurako NEO Yoko FUJII Yoshito WAKAO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(9):1187-1190
Dynamic left ventricular outflow tract obstruction (DLVOTO) is a common condition in cats
and humans. In this case report, a dog is described with DLVOTO secondary to severe
intra-abdominal hemorrhage caused by a hemangiosarcoma. The dog was a 9-year-old, 35.7-kg,
spayed female German Shepard dog that presented with a history of tachypnea and collapse.
A Levine II/VI systolic murmur was present at the heart base. Abdominal ultrasonography
revealed a splenic mass and a large amount of ascites. Echocardiography showed a reduced
left ventricular diameter and an increased aortic velocity caused by systolic anterior
motion (SAM) of the mitral valve apparatus. The heart murmur and the SAM were resolved
after treatment including a splenectomy and a blood transfusion. 相似文献
72.
Ryou Tanaka DVM PhD Katsuichiro Hoshi DVM Yukiko Nagashima DVM Yoko Fujii DVM Yoshihisa Yamane DVM PhD 《Veterinary surgery : VS》2001,30(6):580-584
OBJECTIVE: To describe the use of a detachable coil for transcatheter closure (TCC) of patent ductus arteriosus (PDA) in 2 dogs. STUDY DESIGN: Clinical study. ANIMALS: Two female Pembroke Welsh Corgi dogs with PDA. METHODS: Using fluoroscopic guidance, an 8-mm-diameter coil stent with 5 loops (detachable coils for PDA closure) was inserted via catheterization of the femoral artery. The catheter was passed through the PDA into the pulmonary artery. The coil was withdrawn so that 1.5 loops remained on the pulmonary side of the orifice of the ductus. The rest of the loops were pushed out from the catheter into the ductus. After confirming the correct placement of the coil and the effectiveness of the occlusion, the delivery wire was detached from the coil. RESULTS: Insertion of the coil was easily performed, even without previous experience. Immediate and marked decrease of the cardiac murmur was auscultated. Only slight residual flow was detected by angiography conducted 3 months' postoperatively. The dogs experienced quick and uneventful recovery after coil placement and required minimal postoperative care. Follow-up evaluation of the dogs showed no functional clinical signs of PDA, and no cardiac abnormalities were detected on electrocardiographic, phonocardiographic, and echocardiographic examination. In dog 1, the residual flow had disappeared on the color-flow Doppler echocardiographic examination at 18 months' postoperatively. CONCLUSION: TCC using a detachable coil was easy, safe, and effective in 2 dogs with PDA. The minimal residual shunting observed only by echocardiography seemed hemodynamically insignificant. CLINICAL RELEVANCE: This method can be used as an alternative to traditional surgical methods. 相似文献
73.
Ma Z Mizukoshi T Khatlani TS Okuda M Onishi T 《Veterinary immunology and immunopathology》2000,77(3-4):321-327
The serum amyloid A (SAA) protein is a characteristic and sensitive acute phase reactant in all vertebrates investigated. We molecularly cloned the equine cDNA encoding SAA from the liver of a healthy horse by polymerase chain reaction (PCR). The cloned cDNA is 480 bases in length, and contains an open reading frame (ORF) of 387 nucleotides encoding a precursor SAA protein of 128 amino acids. The precursor of horse SAA seems to have an 18-residue signal peptide and differs from the reported amino acid sequences of the horse SAA by substitution of valine at residue 81. It shows high homology with SAA amino acid sequence of other species such as dog (80.6%), mink (77.5%), human (76.9%) and duck (71.9%). An insertion of eight amino acids at residues between 85 and 92, as compared to human SAA, has also been found in horse SAA. The availability of the equine SAA cDNA will provide a useful reagent for studying its role in diseased horses. 相似文献
74.
75.
76.
Development of a multiplex polymerase chain reaction method for simultaneous detection of eight events of genetically modified maize 总被引:2,自引:0,他引:2
Onishi M Matsuoka T Kodama T Kashiwaba K Futo S Akiyama H Maitani T Furui S Oguchi T Hino A 《Journal of agricultural and food chemistry》2005,53(25):9713-9721
In this study, we developed a novel multiplex polymerase chain reaction (PCR) method for simultaneous detection of up to eight events of genetically modified (GM) maize within a single reaction. The eight detection primer pairs designed to be construct specific for eight respective GM events (i.e., Bt11, Event176, GA21, MON810, MON863, NK603, T25, and TC1507) and a primer pair for an endogenous reference gene, ssIIb, were included in the nonaplex(9plex) PCR system, and its amplified products could be distinguished by agarose gel and capillary electrophoreses based on their different lengths. The optimal condition enabled us to reliably amplify two fragments corresponding to a construct specific sequence and a taxon specific ssIIb in each of the eight events of GM maize and all of nine fragments in a simulated GM mixture containing as little as 0.25% (w/w) each of eight events of GM maize. These results indicate that this multiplex PCR method could be an effective qualitative detection method for screening GM maize. 相似文献
77.
Obana H Okihashi M Akutsu K Kitagawa Y Hori S 《Journal of agricultural and food chemistry》2003,51(9):2501-2505
A rapid and simple extraction method for the simultaneous analysis of five neonicotinoid insecticides has been developed. Twelve different fruit and vegetable matrixes were extracted with methanol and cleaned up using a graphitized carbon solid phase extraction cartridge loading with a 20% methanol solution. The concentrated eluate after methanol elution was then analyzed for pesticide residues by liquid chromatography/mass spectrometry in the APCI positive mode. The five pesticides including nitenpyram, thiamethoxam, imidacloprid, acetamiprid, and thiacloprid were recovered at 70-95% at spike levels of 0.1 and 1 mg/kg in bell pepper, cucumber, eggplant, grape, grapefruit, Japanese radish, peach, pear, potato, rice, and tomato. Relative standard deviations were less than 10% for all of the recovery tests. The proposed method is fast, easy to perform, and could be utilized for regular monitoring of pesticide residues. 相似文献
78.
Tomato plants infected with Potato spindle tuber viroid (PSTVd) had severe leaf curling and vein necrosis. The disease symptoms began to diminish during the late stages of infection, however, and almost healthy-looking leaves began to appear on the upper portion of the plants. PSTVd concentrations reached their highest levels in leaves with severe symptoms and decreased in upper leaves recovering from severe symptoms. PSTVd-specific short interfering RNAs (siRNAs), characteristic of RNA silencing, accumulated in all leaves in which PSTVd reached a detectable level, suggesting that recovery from severe disease was induced by RNA silencing via sequence-specific degradation of PSTVd. 相似文献
79.
Shunsuke TAKAHASHI Yoko YOSHIDA Nobuo NAKANISHI Takamitsu TSUKAHARA Kazunari USHIDA 《Animal Science Journal》2008,79(6):737-744
Levels of fecal or intestinal lactobacilli, Escherichia coli and Clostridium perfringens, and the prevalence of clostridial alpha toxin gene and heat‐stable toxin (ST) gene of enterotoxigenic E. coli (ETEC) were monitored in weaned piglets before (day 0) and during (days 7, 14, and 21) the administration of Lactobacillus plantarum strain Lq80. Lactobacilli were enumerated in a culture‐dependent method. The remainders were determined by quantitative real‐time PCR. In this quantitative real‐time PCR method, the detection limit was proved to be as low as 103 cells/g feces or intestinal contents. Number of lactobacilli increased from day 0 to day 7 (P < 0.05), to day 14 (P < 0.05), and to day 21 (P = 0.07) in the Lq80‐administered group. L. plantarum contributed to as low as 10% of the lactobacillal population in the Lq80‐administered group. The number of E. coli and C. perfringens, and the prevalence of alpha toxin gene in feces or intestinal contents of the Lq80‐administered group decreased, at least in the first week of the postweaning period. Oral administration of L. plantarum strain Lq80 can stimulate the growth of indigenous lactobacilli and decrease ST‐producing ETEC and C. perfringens in the intestine of postweaning piglets. 相似文献
80.
Yoko HAYAMA Takehisa YAMAMOTO Sota KOBAYASHI Norihiko MUROGA Toshiyuki TSUTSUI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(9):1167-1170
The transmission risk of foot-and-mouth disease (FMD) in Japan was evaluated using a
mathematical FMD transmission model. The distance-based transmission rate between farms,
which was parameterized using the FMD epidemic data in 2010 in Japan, was used to
calculate the local-level reproduction numbers—expected numbers of secondary infections
caused by one infected farm—for all cattle and pig farms in the country, which were then
visualized as a risk map. The risk map demonstrated the spatial heterogeneity of
transmission risk in the country and identified risk areas with higher possibility of
disease spread. This result suggests that, particularly in high-risk areas, it is
important to prepare for the smooth and efficient implementation of control measures
against FMD outbreaks. 相似文献