Chemical synthesis of hydroxycinnamic acid glucosides and evaluation of their ability to stabilize natural colors via anthocyanin copigmentation

This work describes the chemical synthesis of O-aryl-beta-D-glucosides and 1-O-beta-D-glucosyl esters of hydroxycinnamic acids. In particular, O-aryl-beta-D-glucosides were efficiently prepared via a simple diastereoselective glycosylation procedure using phase transfer conditions. Despite the lability of its ester linkage, 1-O-beta-D-caffeoylglucose could also be obtained using a Lewis acid catalyzed glycosylation step and a set of protective groups that can be removed under neutral conditions. Hydroxycinnamic acid O-aryl-beta-D-glucosides were then quantitatively investigated for their affinity for the naturally occurring anthocyanin malvin (pigment). Formation of the pi-stacking molecular complexes (copigmentation) was characterized in terms of binding constants and enthalpy and entropy changes. The glucosyl moiety did not significantly alter these thermodynamic parameters, in line with a binding process solely involving the polyphenolic nuclei.     

A comparison of growth, survival rate, and number of marketable fish produced of swordtails, Xiphophorus helleri Heckel (Family Poeciliidae), between two types of culture systems

To test the effect of stocking density on growth, survival rates and size distribution of juvenile swordtails, Xiphophorus helleri Heckel, under different environmental conditions, fish were stocked at rates of 1, 3 and 6 fish I^?1. Growth was monitored over a 14-week period. Fish were weighed in six groups of 20 fish per tank. Size variation was determined at the end of the growth period by measuring the total length of each fish to the nearest mm. Fish were stocked in two different systems at three stocking densities (1, 3, and 6 fish I^?1) in replicate. The systems were an indoor aquarium system and a tunnel system under semicommercial conditions. In each system, increasing stocking rates resulted in a significant reduction of growth rates. In the aquarium system, survival rate was not related to stocking density, whereas in the tunnel system, fish at the highest stocking density had a lower survival rate than those kept at the two lower stocking densities. The number of marketable fish above a set size limit of 40 mm total length was not higher in fish stocked at 6 fish I^?1 than at 3 fish I^?1 at the end of the study. Therefore, a stocking rate of 3 fish I^?1 would be optimal. At the end of the study, fish were categorized into males, females and immature fish. Fish with a clearly visible gonopodium were classified as males, those with typical female characteristics as females, and small fish that were not yet differentiated as immature fish. The ratio of females to males was highly skewed in both systems, favouring a higher percentage of females. As stocking density increased, the female:male ratio was significantly reduced, which would result in a greater number of saleable pairs at increased stocking density. The implications of these results for the management of intensive production of swordtails are discussed.     

LOW‐FIELD MAGNETIC RESONANCE IMAGING APPEARANCE OF POSTARTHROSCOPIC MAGNETIC SUSCEPTIBILITY ARTIFACTS IN HORSES

An awareness of magnetic susceptibility artifacts is important for interpreting prepurchase and postoperative magnetic resonance imaging (MRI) studies in horses. These artifacts occur when a metallic or a paramagnetic substance creates a local magnetic field deformity. Aims of the current experimental study were to determine prevalence of these artifacts after arthroscopy in a sample of nonlame horses, and to describe effects of time and type of pulse sequence on low‐field MRI signal intensity and detection of the artifacts. Ten, nonlame Standardbred horses were prospectively recruited. All horses underwent arthroscopy of both metacarpophalangeal joints for purposes unrelated to the study. Serial low‐Field MRI examinations were performed on each horse and each joint (before, and 6 and 12 weeks postsurgery). In two horses, more detailed longitudinal evaluations were performed with additional MRI examinations. Magnetic susceptibility artifacts were detected postoperatively at the surgical access sites in eight metacarpophalangeal joints at both 6 and 12 weeks after surgery (40% prevalence). Neither of the two longitudinally followed horses had artifacts at any time. Artifacts were only detected on gradient echo (GRE) sequences. Findings indicated that magnetic susceptibility artifacts can be present in postarthroscopy MRI studies in horses and can persist up to 12 weeks after arthroscopy. For this sample of horses, the artifacts did not interfere with evaluation of the joint. Further longitudinal studies are needed to determine the full duration of magnetic susceptibility artifact persistence in affected tissues.     

Présence de la maladie bactérienne du rein chez les salmonidés au Québec

Presence of the bacterial kidney disease in salmonid fish in Quebec During the summers of 1979 and 1980, wild and hatchery fish were analysed for the presence of the bacterial kidney disease (BKD) agent in salmonid fish in Quebec by the indirect immunofluorescence technique. The causative agent of BKD was detected in all hatcheries tested. Ten to 25% of the fish were positive. The presence of this agent was independent of age and species. We were unable to detect the BKD in fish from the rivers in the northern part of Quebec (over the 50th parallel).