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Obtaining Wisent early blastocyst in vitro is a basic for protection and creation of biodiversity for this threatened species 下载免费PDF全文
AM Duszewska P Gręda M Baraniewicz W Bielecki W Niżański A Partyka M Tracz Z Nowak A Chełmońska‐Soyta W Olech 《Reproduction in domestic animals》2018,53(3):818-821
Wisent, or European bison (Bison bonasus), is listed as “vulnerable” on the IUCN Red List of Threatened Species and is therefore protected by international law. For the first time, a Wisent embryo has been obtained in vitro. This procedure creates a new opportunity to protect and increase Wisent reproductive potential and thereby opens new possibilities for the establishment of a controlled and broad reserve of the gene pool. 相似文献
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J Li K Villemoes Y Zhang Y Du PM Kragh S Purup Q Xue AM Pedersen AL Jørgensen JE Jakobsen L Bolund H Yang G Vajta 《Reproduction in domestic animals》2009,44(1):122-127
The purpose of our work was to establish an efficient-oriented enucleation method to produce transgenic embryos with handmade cloning (HMC). After 41–42 h oocytes maturation, the oocytes were further cultured with or without 0.4 μg/ml demecolcine for 45 min [chemically assisted handmade enucleation (CAHE) group vs polar body (PB) oriented handmade enucleation (OHE) group respectively]. After removal of the cumulus cells and partial digestion of the zona pellucida, oocytes with visible extrusion cones and/or polar bodies attached to the surface were subjected to oriented bisection. Putative cytoplasts without extrusion cones or PB were selected as recipients. Two cytoplasts were electrofused with one transgenic fibroblasts expressing green fluorescent protein (GFP), while non-transgenic fibroblasts were used as controls. Reconstructed embryos were cultured in Well of Wells (WOWs) with porcine zygote medium 3 (PZM-3) after activation. Cleavage and blastocyst rates were registered on day 2 and day 7 of in vitro culture respectively. Meanwhile, the total blastocyst cell number was counted on day 7. We found that the difference was only observed between blastocyst rates (38.6 ± 2% vs 48.1 ± 3%) of cloned embryos with GFP transgenic fibroblast cells after CAHE vs OHE. With adjusted time-lapse for zonae-free cloned embryos cultured in WOWs with PZM-3, it was obvious that in vitro developmental competence after CAHE was compromised when compared with the OHE method. OHE enucleation method seems to be a potential superior alternative method used for somatic cell nuclear transfer (SCNT) with transgenic fibroblast cells. 相似文献
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Mitochondrial Activity, Distribution and Segregation in Bovine Oocytes and in Embryos Produced in Vitro 总被引:7,自引:0,他引:7
AM Tarazona JI Rodríguez LF Restrepo M Olivera-Angel 《Reproduction in domestic animals》2006,41(1):5-11
Contents Bovine oocytes and embryos produced in vitro were studied to determine the mitochondrial pattern of distribution, segregation and activity using DIOC 6 and Jc-1 fluorescence. The highest fluorescence level observed in mature oocytes was taken as 100% activity and six activity levels were estimated as follows: (1) 0%, (2) 1-15%, (3) 16-30%, (4) 31-50%, (5) 51-75% and (6) 76-100%. Three patterns of mitochondrial distribution were found: (1) diffused throughout the cytoplasm in oocytes and embryos, (2) pericytoplasmic in oocytes and embryos, and (3) perinuclear only in embryos. The segregation of mitochondria in blastomeres showed two distinct patterns: (1) symmetrical with an even mitochondrial population, and (2) asymmetrical with different numbers of mitochondria in each blastomere. In immature oocytes, mitochondrial activity was very low and the distribution was diffuse or negligible, while in mature oocytes the activity was high and the distribution was diffuse or pericytoplasmic. Competent embryos up to the 16-cell stage showed intermediate levels of activity (16-50%) but activity decreased thereafter up to the blastocyst stage. Non-competent embryos showed low levels of activity (1-15%) at all stages. These results suggest that mitochondria might play an important role during early development and that a minimum threshold of activity regulates the potential competence for reaching the blastocyst stage. 相似文献
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Shearing male lambs in the cold season improves the carcass yield without affecting fattening performance 总被引:1,自引:0,他引:1
This research was carried out to determine the effects of shearing of different genotypes of male lambs (one from pure Karayaka stock and two from its crossbreed) in winter on bodyweight gain, feed consumtion, feed conversion efficiency, rectal temperature and carcass yield. A total of 21 lambs aged 8 months were allocated randomly to experimental groups according to a 2 × 3 factorial arrangement for shorn and unshorn animals. The lambs were fed grass hay (100 g/lamb/day) and commercial concentrate feed ad libitum during a 54‐day of fattening period. The lambs were shorn in the middle of the experiment (27 days after beginning the trial). The interaction between the shearing treatment × genotype of the studied parameters was found to be insignificant. The shearing process increased the rectal temperature (P < 0.01), hot carcass yield (P < 0.05) and dressing percentage (P < 0.05) and decreased the weight of the edible inner organs (P < 0.01). Some of these parameters were affected by the genotypes. It was concluded that shearing male lambs in the winter can have a beneficial effect on the hot carcass weight and dressing percentage without affecting fattening performance, and the performance of Karayaka lambs and its crossbred male lambs were similar under the conditions of the present study. 相似文献
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Objective To determine whether dogs and cats are potential reservoirs of Ross River (RR) and Barmah Forest (BF) viruses
Method Young seronegative female dogs and cats were experimentally exposed to the viruses using Ochlerotatus vigilax (Skuse) mosquitoes.
Results Only one of the 10 dogs and one of the 10 cats exposed to RR developed neutralising antibody. None of the animals developed detectable viraemia or clinical signs. One dog and three cats exposed to BF developed neutralising antibody. In addition, a serological survey of sera obtained from domestic dogs and cats residing in the Brisbane region indicated that 23.7% and 1.3% of dogs, and 14% and 2% of cats, had neutralising antibodies to RR and BF respectively.
Conclusions Although dogs and cats are exposed naturally to these viruses, and can become infected, they are unlikely to be important urban reservoirs of either virus. 相似文献
Method Young seronegative female dogs and cats were experimentally exposed to the viruses using Ochlerotatus vigilax (Skuse) mosquitoes.
Results Only one of the 10 dogs and one of the 10 cats exposed to RR developed neutralising antibody. None of the animals developed detectable viraemia or clinical signs. One dog and three cats exposed to BF developed neutralising antibody. In addition, a serological survey of sera obtained from domestic dogs and cats residing in the Brisbane region indicated that 23.7% and 1.3% of dogs, and 14% and 2% of cats, had neutralising antibodies to RR and BF respectively.
Conclusions Although dogs and cats are exposed naturally to these viruses, and can become infected, they are unlikely to be important urban reservoirs of either virus. 相似文献
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Firing patterns of single neurons in the hypothalamus, preoptic area, midbrain reticular system, and hippocampus of awake, freely moving female rats were temporally correlated with exploratory sniffing and vibrissa twitching, feeding, lordosis, locomotion, and (or) arousal. These relationships were remarkably stable during continuous observations lasting many hours. During extended periods when certain of these movements were not performed, the correlated neurons showed no action potentials for minutes at a time. Electrical stimulation at certain recording sites elicited behavior patterns whose spontaneous occurrence was accompanied by neuronal activation. Self-stimulation was elicited from sites spontaneously activated during exploratory behavior. 相似文献