Investigation of the occurrence of Angiostrongylus vasorum in coyotes in southern Ontario,Canada

In North America, the only endemic focus for Angiostrongylus vasorum (French heartworm) was historically thought to occur in the southeastern part of the island of Newfoundland. However, reports of A. vasorum infection in wild canids in West Virginia, USA, and Nova Scotia, Canada, suggest the introduction of the parasite to mainland North America. We screened for A. vasorum in coyotes from across southern Ontario. Additionally, we evaluated the performance of ELISAs for detection of circulating A. vasorum antigen (Ag-ELISA) and antibodies against A. vasorum (Ab-ELISA) designed for use in sera or blood of foxes for use with coyotes in this region. Autopsies were performed on 397 coyotes, and lung tissue extract prepared from each carcass was tested via both ELISAs. The sensitivity and specificity for both tests were estimated in the absence of a gold standard using a 2-test single population Bayesian model; sensitivity and specificity priors were based on the performance of the assays in foxes in Switzerland. Eight coyotes tested positive for A. vasorum antigen; no animal was antibody positive. The estimated sensitivity and specificity of the Ag-ELISA were 90.8% (95% credible interval [CrI]: 83.8–95.6%) and 95.5% (95% CrI: 93.4–97.2%), respectively. For the Ab-ELISA, the estimated sensitivity and specificity were 41.9% (95% CrI: 32.1–51.9%) and 98.0% (95% CrI: 96.3–99.0%), respectively. Based on these findings and negative postmortem data for the same animals, there is insufficient evidence to suggest the presence of A. vasorum in southern Ontario coyotes.     

Modeling the timber supply impact of introducing weevil-resistant spruce in British Columbia with cellular automata

Assessing the impacts of cumulative, non-lethal damages to forests poses a particular challenge to forest management planning since cumulative merchantable volume losses are not known until the time of harvest. This paper introduces a model that scales stand-level processes to their large-scale implications by combining elements of growth and yield modeling, insect infestation modeling, and large-scale spatial harvest simulation. The model is described and tested using the proposed large-scale introduction of weevil-resistant spruce in British Columbia, Canada as a case study. While spruce weevil damages were found to be significant, only a relatively small percentage of losses can be avoided by planting resistant stock. The model introduced in this paper can serve as an important decision support tool for policy makers and researchers in assessing the large-scale effects of proposed policy and management interventions.     

Inhibition of hepatitis B virus replication by drug-induced depletion of nucleocapsids

Chronic hepatitis B virus (HBV) infection is a major cause of liver disease. Only interferon-alpha and the nucleosidic inhibitors of the viral polymerase, 3TC and adefovir, are approved for therapy. However, these therapies are limited by the side effects of interferon and the substantial resistance of the virus to nucleosidic inhibitors. Potent new antiviral compounds suitable for monotherapy or combination therapy are highly desired. We describe non-nucleosidic inhibitors of HBV nucleocapsid maturation that possess in vitro and in vivo antiviral activity. These inhibitors have potential for future therapeutic regimens to combat chronic HBV infection.     

A PINOID-dependent binary switch in apical-basal PIN polar targeting directs auxin efflux

Polar transport-dependent local accumulation of auxin provides positional cues for multiple plant patterning processes. This directional auxin flow depends on the polar subcellular localization of the PIN auxin efflux regulators. Overexpression of the PINOID protein kinase induces a basal-to-apical shift in PIN localization, resulting in the loss of auxin gradients and strong defects in embryo and seedling roots. Conversely, pid loss of function induces an apical-to-basal shift in PIN1 polar targeting at the inflorescence apex, accompanied by defective organogenesis. Our results show that a PINOID-dependent binary switch controls PIN polarity and mediates changes in auxin flow to create local gradients for patterning processes.     

Zero tillage impacts in India's rice–wheat systems: A review

To date, the most widely adopted resource conserving technology in the Indo-Gangetic Plains (IGP) of South Asia has been zero-tillage (ZT) wheat after rice, particularly in India. The paper reviews and synthesizes the experience with ZT in the Indian IGP. ZT wheat is particularly appropriate for rice–wheat systems in the IGP by alleviating system constraints by allowing earlier wheat planting, helping control the weed Phalaris minor, reducing production costs and saving water. ZT wheat after rice generates substantial benefits at the farm level (US$97 ha⁻¹) through the combination of a ‘yield effect’ (a 5–7% yield increase, particularly due to more timely planting of wheat) and a ‘cost savings effect’ (US$52 ha⁻¹, particularly tillage savings). These benefits explain the widespread interest of farmers and the rapidity of the diffusion across the Indian IGP, further aided by the wide applicability of this mechanical innovation.     

Rapid formation of sulfuric acid particles at near-atmospheric conditions

We investigated the formation of new particles in a laboratory study, starting from H2SO4 produced in situ through the reaction of OH radicals with SO2. Newly formed particles were observed for H2SO4 concentrations above 7 x 10(6) per cubic centimeter. At 293 kelvin, a rough estimate yielded a nucleation rate of 0.3 to 0.4 particles per cubic centimeter per second for approximately 10(7) particles per cubic centimeter of H2SO4 (particle size >/= 3 nanometers). These findings are in agreement with observations from the atmosphere. The results demonstrate that under laboratory conditions similar to the atmosphere, particle formation occurs at atmospheric H2SO4 concentration levels.