Melanized focal changes in skeletal muscle in farmed Atlantic salmon after natural infection with Piscine orthoreovirus (PRV)

Melanized focal changes in skeletal muscle of farmed Atlantic salmon (Salmo salar) are a major quality problem. The aetiology is unknown, but infection with Piscine orthoreovirus (PRV) has been associated with the condition. Here, we addressed the pathogenesis of red and melanized focal changes and their association with PRV. First, a population of farmed fish (PRV‐negative prior to sea transfer) was sequentially investigated throughout the seawater period. The fish were autopsied and tested for PRV infection. Muscular changes were described by macroscopy and histology, and a classification system was established. Second, in an experimental infection trial, PRV was injected intramuscularly to induce changes. The farmed fish was gradually infected with PRV. Red focal changes occurred throughout the observation period with a low prevalence regardless of PRV status. Melanized changes were highly diverse and their prevalence increased during the trial. Changes of low macroscopic grade and histological category were more prevalent in PRV‐negative fish. Diffuse granulomatous melanized changes only occurred after PRV infection. No muscular changes were observed in the experimentally challenged fish. Our studies do not indicate that PRV infection causes red focal changes, but seems important in the development of granulomatous melanized changes.     

Genetic variation in resistance to infectious pancreatic necrosis in rainbow trout (Oncorhynchus mykiss) after a challenge test

In this study, we wanted to evaluate genetic variation in resistance to infectious pancreatic necrosis (IPN) in a breeding population of rainbow trout. Two hundred families were challenged at a commercial test station, using methods that were previously developed for testing resistance to IPN in Atlantic salmon. Thirty‐five days after the challenge the accumulated mortality was 26%. The results show that resistance to IPN is moderately heritable in the tested population (h²=0.30). The genetic correlation between IPN resistance and body weight was found to be low and non‐significant. The significant additive genetic variation found in IPN resistance after a controlled challenge test gives promise for successful breeding for increased resistance to IPN in rainbow trout.     

Comparing water management in rice-wheat production systems in Haryana, India and Punjab, Pakistan

The intensive irrigated rice-wheat systems in the northwest Indo-Gangetic Plains of South Asia are built on a long tradition of canal irrigation and the more recent advent of tubewells. Findings from farm surveys are used to examine water management and water productivity in the rice-wheat belt of India's Haryana State and Pakistan's Punjab province. Attributes of the irrigation sources help explain the widespread interest in groundwater use and the relative demise of canal water use. In each area groundwater now is the main irrigation source, used either solely or in conjunction with surface water. The ownership of tubewells is near universal among the surveyed farms, whereas conjunctive water use is more widespread during the monsoon season, among better endowed farmers and in the Pakistan Punjab. In Pakistan Punjab farmers primarily rely on diesel powered tubewells whereas Haryana farmers mainly use relatively cheaper electric power. Water productivity indicators for rice are markedly lower than those for wheat—largely reflecting significantly higher water inputs in paddy cultivation—but also vary between the study areas and by the prevailing water use, reflecting the limited incentives for farmers to use water wisely. A combination of technological, land use and market based approaches is likely to be most effective in achieving sustainable water management in these intensive cereal systems.     

Pelagic–littoral resource polymorphism in Hovsgol grayling Thymallus nigrescens from Lake Hovsgol,Mongolia

Resource polymorphism is a widespread phenomenon in post‐glacial fishes where multiple morphotypes of a species occur sympatrically and exhibit distinct resource use. Availability of open niches and high levels of within and among species competition are thought to drive differences in morphology and may provide insights into early stages of speciation. Hovsgol grayling (Thymallus nigrescens) are endemic to Lake Hovsgol, a lake colonised by fish following the Pleistocene, and are threatened with habitat loss due to climate change and illegal harvest. Previous analysis of Hovsgol grayling diet inferred through C and N stable isotopes suggested the presence of littoral and pelagic foraging groups. We hypothesised that morphology of the two foraging groups would reflect predictions of functional morphology, indicating the presence of resource polymorphism. To test this hypothesis, we evaluated evidence from C and N stable isotopes, stomach contents, fish ages, capture location and morphology. Two foraging groups of Hovsgol grayling were identified through stomach content and C and N stable isotope analysis. Individuals with greater zooplankton consumption were more frequently captured in the pelagic zone, exhibited higher gill raker counts, larger orbit (eye) sizes, longer paired fins, narrower head width, larger maxilla and smaller size at age than the group with greater reliance on benthic invertebrate prey. These differences were generally consistent with those described in other fish species exhibiting littoral–pelagic resource polymorphism. Our study provides the first example of littoral–pelagic resource polymorphism in the salmonid subfamily Thymallinae and highlights the potential influence of competition on fish evolution.     

Rapid formation of sulfuric acid particles at near-atmospheric conditions

We investigated the formation of new particles in a laboratory study, starting from H2SO4 produced in situ through the reaction of OH radicals with SO2. Newly formed particles were observed for H2SO4 concentrations above 7 x 10(6) per cubic centimeter. At 293 kelvin, a rough estimate yielded a nucleation rate of 0.3 to 0.4 particles per cubic centimeter per second for approximately 10(7) particles per cubic centimeter of H2SO4 (particle size >/= 3 nanometers). These findings are in agreement with observations from the atmosphere. The results demonstrate that under laboratory conditions similar to the atmosphere, particle formation occurs at atmospheric H2SO4 concentration levels.     

Piscine orthoreovirus (PRV) in red and melanised foci in white muscle of Atlantic salmon (Salmo salar)

Melanised focal changes (black spots) are common findings in the white skeletal muscle of seawater-farmed Atlantic salmon (Salmo salar). Fillets with melanised focal changes are considered as lower quality and cause large economic losses. It has been suggested that red focal changes (red spots) precede the melanised focal changes. In the present work, we examined different populations of captive and wild salmon for the occurrence of both types of changes, which were investigated for the presence of different viruses by immunohistochemistry and RT-qPCR. The occurrence of red or melanised foci varied significantly between the populations, from none in wild fish control group, low prevalence of small foci in fish kept in in-house tanks, to high prevalence of large foci in farm-raised salmon. Large amounts of Piscine orthoreovirus (PRV) antigen were detected in all foci. No other viruses were detected. Red focal changes contained significantly higher levels of PRV RNA than apparently non-affected areas in white muscle of the same individuals. Some changes displayed a transient form between a red and melanised pathotype, indicating a progression from an acute to a chronic manifestation. We conclude that PRV is associated with the focal pathological changes in the white muscle of farmed Atlantic salmon and is a premise for the development of focal melanised changes.     

Occurrence,resistance factors and cross-resistance patterns to herbicides inhibiting acetolactate synthase (ALS) of Echinochloa crus-galli (L.) Pal. Beauv. in Central Europe

Journal of Plant Diseases and Protection - Echinochloa crus-galli (L.) Pal. Beauv. is one of the most noxious weeds in maize cultivation and has evolved target-site resistance to ALS-inhibiting...