Alfalfa saponins and microbial transformations of nitrogen in peat

Alfalfa saponins, their sugar fraction or glucose, but not their sapogenin fraction, favored nitrogen immobilization and denitrification and inhibited proteolysis and ammonification in both peatinoculated and Pseudomonas sp.-inoculated media. Alfalfa root saponins and sapogenins significantly reduced the fungal peat population, but did not affect the bacterial population. The inhibition of N mineralization in peat by saponins and by their sapogenin fraction during dry and wet cycles was mostly due to their fungistatic activity. The inhibitory effect of the sugar fraction of alfalfa roots on mineral-N accumulation in peat is mainly under moist conditions.     

Crop loss of pepper plants artificially infected with Xanthomonas campestris pv. vesicatoria in relation to symptom expression

Crop losses in peppers artificially infected with Xanthomonas campestris pv. vesicatoria (XCV) were determined during 3 years of field experiments in three areas of Israel. Direct losses of 23–44% in fruit yield were recorded when severe leaf infection occurred or was induced at an early stage of plant growth. Yield losses and disease index were markedly lower in plants inoculated at later stages and near maturation. Indirect losses in severely infected fields were mainly due to shedding of leaves and exposure of fruits to sun. In this case, up to 95% of the fruits lost their commercial value. In artificially infected symptomless plants with massive endogenous populations of XCV in the leaves, a loss of 24% in yield was measured, compared with plants free from an endogenous pathogen population.     

Plant-Growth Promotion in Natural Pastures by Inoculation with Azospirillum brasilense Under Suboptimal Growth Conditions

Inoculation experiments of natural pastures with the rhizobacterium Azospirillum brasilense were conducted in Israel in 1996, in two climatically differentiated regions. These experiments were carried out as part of a project with the objective of estimating the potential of the inoculation technique in improving pasture yields. The effects of inoculation with A. brasilense were compared to P-fertilization and to untreated controls. In two semiarid sites, it was generally observed that both the inoculation and the P-fertilization treatments increased pasture yield as measured by dry weight biomass. It was also found that both inoculation and P-fertilization treatments caused an enrichment in legumes relatively to grasses and forbs. Despite the ecological complexity of these sites and the variability of the data, statistical significance was generally achieved. In a more topographically uniform site, located in a typical Mediterranean climate, the positive effects of A. brasilense inoculation in plant-growth were substantially clear, with final yields duplicated in both inoculation and combined (inoculation and P-fertilization) treatments in comparison to P-fertilization alone and untreated controls. The potential of using A. brasilense for growth promotion of natural pastures is discussed.     

Reproducible induction of cavity spot in carrots and physiological and microbial changes occurring during cavity formation

Inoculation of carrots with 40 types of bacteria, both aerobic and anaerobic, including clostridia isolated from cavity spots, failed to induce cavity spot in carrots. A combined stress of minimum 6 h flooding and temperatures higher than 28°C clearly induced cavity formation. Sugars, amino acids, lipids and minerals leaked from the carrots after flooding and heating the roots. A longer growth period following stress markedly increased cavity spots. Soil types (sandy loam and loess) and several carrot cultivars tested had no marked effect on spot formation. Cavities were formed in stressed carrots grown in sterilized soil containing only one type of bacterium, a Gram-negative short rod. Scanning electron micrographs revealed that after carrots were subjected to combined stress, microscopic cavities nearly free of bacteria were formed under the epidermis. Proliferation of bacteria was concommitant with the appearance of visible cavities. Cell-free extracts of infected carrots showed higher protease and pectinase-specific activities, as well as significantly higher peroxidase and polyphenoloxidase activities and total phenol content as compared to healthy carrots.     

Effect of Furolan on the pigment, IAA, protein and total nitrogen contents of germinal leaves of winter wheat

The winter wheat seeds (of three varieties namely Batko, Diya and Krasnodar 99) were germinated and investigated for the effect of growth regulator, Furolan on the pigment, IAA and total nitrogen and protein contents of their germinal leaves. Results showed differences in pigment contents of the germinal leaves of winter wheat varieties studied. Furolan increased the contents of chlorophyll a in all the varieties studied by the range of 22.7–26.4%. The amount of chlorophyll b in Diya’s variety increased by 22.2% while the amount of contents of carotenoids in Batko variety increased by 43.5% in comparison with the control. The highest amount of chlorophyll a and b was recorded in Batko and the least in Diya variety. The reverse was the case with the carotenoids: Furolan increased the amount of carotenoids in all the varieties; the highest amount of carotenoids was observed in Diya variety and Batko variety recorded the least amount In all the varieties studied the Furolan increased the IAA, total nitrogen and protein contents in comparison to the control.     

Induced resistance to foliar diseases by soil solarization and Trichoderma harzianum

The effect of soil solarization and Trichoderma harzianum on induced resistance to grey mould (Botrytis cinerea) and powdery mildew (Podosphaera xanthii) was studied. Plants were grown in soils pretreated by solarization, T. harzianum T39 amendment or both, and then their leaves were inoculated with the pathogens. There was a significant reduction in grey mould in cucumber, strawberry, bean and tomato, and of powdery mildew in cucumber, with a stronger reduction when treatments were combined. Bacillus, pseudomonad and actinobacterial communities in the strawberry rhizosphere were affected by the treatments, as revealed by denaturing gradient gel electrophoresis fingerprinting. In tomato, treatments affected the expression of salicylic acid (SA)‐, ethylene (ET)‐ and jasmonic acid (JA)‐responsive genes. With both soil treatments, genes related to SA and ET – PR1a, GluB, CHI9 and Erf1 – were downregulated whereas the JA marker PI2 was upregulated. Following soil treatments and B. cinerea infection, SA‐, ET‐, and JA‐related genes were globally upregulated, except for the LOX genes which were downregulated. Upregulation of the PR genes PR1a, GluB and CHI9 in plants grown in solarized soil revealed a priming effect of this treatment on these genes' expression. The present study demonstrates the capacity of solarization and T. harzianum to systemically induce resistance to foliar diseases in various plants. This may be due to either a direct effect on the plant or an indirect one, via stimulation of beneficial microorganisms in the rhizosphere.     

Effect of storage time and Furolan on the contents of nitrogen, protein and non protein nitrogen in winter wheat grain

This study was carried out to investigate the effect of storage time and growth regulator, Furolan on protein, non-protein nitrogen and nitrogen contents of three varieties of winter wheat grains namely Batko, Diya and Krasnodar 99. The contents of the nitrogen, protein and non-protein nitrogen in winter wheat grain were determined by different protocols during the full ripeness phase of the grain and the period of 12 months storage. The grains (both the control and experimental) were kept in storage for varied periods under normal conditions. Krasnodar 99 variety responded most to the Furolan and recorded an increase in protein nitrogen content by 46.3% followed by 24.9% in experimental Batko variety and 17.4% in Diya??s variety in comparison with the control. The content of protein nitrogen in the grain of experimental Batko variety increased up to the seventh month of storage, and then decreased by the 12th month of storage. In the control??a similar tendency of change in this parameter was observed. It was noted that seven months of storage is critical in change dynamics of the content of non-protein nitrogen in the selected winter wheat varieties studied.     

Contribution of nitrogen fixed by Azospirillum to the n nutrition of spring wheat in Israel

Acetylene reduction activity (ARA) was measured in cores containing roots of various Israeli wild and cultivated wheat lines colonized by Azospirillum. The inoculated plants were grown under greenhouse or field conditions. Although, no measurable ARA was detected during earlier stages of wheat development, 50–600 nmol C₂H₄ g^?1 dry root h^?1 was measured during heading and flowering stages. By using N yield balance and ¹⁵N dilution techniques, it was found that Triticum aestivum cv. Miriam inoculated with Azospirillum accumulated 20% more N (¹⁴N and ¹⁵N) at the booting stage than did the uninoculated control. This difference in N content became less apparent in grains. No significant ¹⁵N dilution could be found and the contribution of atmospheric N₂ to the N content of grains of inoculated plants was negligible. It was concluded that the potential contribution of biological N₂ fixation to spring wheat cultivation in Israel is very low.