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441.
442.
Park BS Kim JG Kim MR Lee SE Takeoka GR Oh KB Kim JH 《Journal of agricultural and food chemistry》2005,53(23):9005-9009
The inhibitory activity of Curcuma longa L. (turmeric) rhizome constituents against sortase A, a bacterial surface protein anchoring transpeptidase, from Staphylococcus aureus ATCC 6538p was evaluated. The activity of the isolated compounds (1-4) was compared to that of the positive control,p-hydroxymecuribenzoic acid (pHMB). The biologically active components of C. longa rhizome were characterized by spectroscopic analysis as the curcuminoids curcumin (1), demethoxycurcumin (2), and bisdemethoxycurcumin (3). Curcumin was a potent inhibitor of sortase A, with an IC50 value of 13.8 +/- 0.7 microg/mL. Bisdemethoxycurcumin (IC50 = 31.9 +/- 1.2 microg/mL) and demethoxycurcumin (IC50 = 23.8 +/- 0.6 microg/mL) were more effective than pHMB (IC50 = 40.6 +/- 1.2 microg/mL). The three isolated compounds (1-3) showed no growth inhibitory activity against S. aureus strain Newman, with minimum inhibitory concentrations (MICs) greater than 200 microg/mL. Curcumin also exhibited potent inhibitory activity against S. aureus cell adhesion to fibronectin. The suppression of fibronectin-binding activity by curcumin highlights its potential for the treatment of S. aureus infections via inhibition of sortase activity. These results indicate that curcumin is a possible candidate in the development of a bacterial sortase A inhibitor. 相似文献
443.
Kim Ki-Hyun Sung Lee Hak Youn Yong-Hoon Yun Seong-Taek Ro Chul-Un Oh Jong-Min 《Water, air, and soil pollution》2002,138(1-4):7-24
The concentrations of seven metals (Fe, Pb, Mn, Ni, Cd, Cu, and Cr) associated with the total suspended particles (TSPs) were analyzed regularly from the four sampling sites of different land-use types within the boundary of Won Ju city, Korea during1991 through 1995. The mean concentration data for the four sitesselected to represent grassland, residential, commercial, and industrial areas fell in a relatively broad range of: 1440–2240 (Fe), 88–326 (Pb), 2–4 (Cd), 8–21 (Cr),194–469 (Cu), 32–95 (Mn), and 15–26 (Ni) ng m-3. The data, when compared across the different study sites, generally exhibited systematic trends in accordance withthe site-selection scheme; most metals exhibited increase in their concentration levels across grassland through industrial site. Examinations of data also indicated the possibility that spatial factors play complicated roles on both long- and short-term distribution of metals. From all four sites studied, severalmetals (e.g., Fe, Pb, Mn, Ni, and Cd) consistently showed theirseasonal trends characteristic of high winter/spring values. In addition, the analysis of long-term distribution trendsindicated that the concentrations of many metals droppedsteadily at four sites (e.g., Fe, Pb, and Mn). When these metaldata were compared among different sites (land-use types), significant correlations were seen frequently for such metals asFe, Pb, Cd, and Cr. Investigations of inter-metal relationshipsindicated that strong correlations were more abundant from such metal pairs as Fe-Pb, Pb-Mn, and Mn-Fe. In addition, the cases for such strong correlations were seen more abundantly from grassland (and residential) than industrial (and commercial) site. Factor analysis was also conducted to distinguish sourceprocesses affecting metal distributions in the study area. Results of this analysis suggest that the metal distributions ofthe individual sites may be affected most significantly by distinctive source processes of their own. 相似文献
444.
Dan Lee Jae Young Oh Samuth Sum Hee-Myung Park 《Journal of veterinary science (Suw?n-si, Korea)》2021,22(2)
BackgroundKlebsiella spp. is an important conditional pathogen in humans and animals. However, due to the indiscriminate use of antibiotics, the incidence of antimicrobial resistance has increased.ObjectivesThe purpose of this study was to investigate antimicrobial resistance in strains of Klebsiella strains and the phylogenetic relatedness of extended-spectrum cephalosporin (ESC)-resistance among Klebsiella strains isolated from clinically ill companion animals.MethodsA total of 336 clinical specimens were collected from animal hospitals. Identification of Klebsiella species, determination of minimum inhibitory concentrations, detection of ESC resistance genes, polymerase chain reaction-based replicon typing of plasmids by conjugation, and multilocus sequence typing were performed.ResultsForty-three Klebsiella strains were isolated and, subsequently, 28 were identified as K. pneumoniae, 11 as K. oxytoca, and 4 as K. aerogenes. Eleven strains were isolated from feces, followed by 10 from ear, 7 from the nasal cavity, 6 from urine, 5 from genitals, and 4 from skin. Klebsiella isolates showed more than 40% resistance to penicillin, cephalosporin, fluoroquinolone, and aminoglycoside. ESCresistance genes, CTX-M groups (CTX-M-3, CTX-M-15, and CTX-M-65), and AmpC (CMY-2 and DHA-1) were most common in the K. pneumoniae strains. Some K. pneumoniae carrying CTX-M or AmpC were transferred via IncFII plasmids. Two sequence types, ST709 and ST307, from K. pneumoniae were most common.ConclusionsIn conclusion, this is the first report on the prevalence, ESCresistance genotypes, and sequence types of Klebsiella strains isolated from clinically ill companion animals. The combination of infectious diseases and antimicrobial resistance by Klebsiella in companion animals suggest that, in clinical veterinary, antibiotic selection should be made carefully and in conjunction with the disease diagnosis. 相似文献
445.
Ozone, generated by a pulsed power corona discharge, was usedto degrade pentachlorophenol (PCP). The degradation of PCP wasdriven by ozone and superoxide radical (SOR). The coronadischarge reactor was made without glass dielectric material,not only to reduce the power consumption but also to enhancethe formation of the SOR. In addition, the corona dischargedevice was operated with pin-plate or pin-ring typeelectrodes, which had a normal and parallel oxygen flow,respectively. The ozone generated in the corona dischargereactor was in the range of 0-0.39 mg min-1. To confirm the generation of SOR, the hydrogen peroxide was measuredunder acidic conditions without ozone effects. PCP at 10 ppm wascompletely degraded in 10 min in both electrode types withnegative high DC voltage. The degradation rate of PCP was muchgreater at a high frequency (1 kHz). Oxalic, malonic andglyoxylic acids were generated as final products of the reaction. 相似文献
446.
Jae-Cheol Nam Sung-Nam Oh Jae-Cheon Choi Jiyoung Kim Youngsin Chun 《Water, air, and soil pollution》2001,130(1-4):433-438
In order to investigate the temporal and spacial deposition characteristics of acid rain, five monitoring sites were set up in the central part of Korean Peninsula. Rainwater samples were collected by wet-only sampler from 1992 to 1997. The pH and conductivity values were measured and the major water soluble ionic components were also analyzed. Ion balance between anion and cation sum was calculated to check out the data quality of samples. The acidity of rainwater was influenced by the local sources and meteorological factors such as surface wind, moving path of storm, precipitation process, and so on. Backward trajectories were depicted to investigate the effect due to moving path of weather system. The rainwater shows strong acidity in winter and weak in summer. The pH and ion concentrations were highly dependent on synoptic weather system. In addition, we sampled fog/cloud at Mt. Sobaek (BAPMoN Station) from June to August 1995 and characterized the fog/cloud chemistry according to surface wind system of sampling site. The volume-weighted mean pH of fog/cloud samples is 4.39 at Mt. Sobaek during the sampling period. 相似文献
447.
Many apoptotic molecules relocate subcellularly in cells undergoing apoptosis. The pro-apoptotic protein BID underwent posttranslational (rather than classic cotranslational) N-myristoylation when cleavage by caspase 8 caused exposure of a glycine residue. N-myristoylation enabled the targeting of a complex of p7 and myristoylated p15 fragments of BID to artificial membranes bearing the lipid composition of mitochondria, as well as to intact mitochondria. This post-proteolytic N-myristoylation serves as an activating switch, enhancing BID-induced release of cytochrome c and cell death. 相似文献
448.
Recent results with Tokamak experiments provide insights into the problem of magnetic confinement. They demonstrate how to avoid anomalous transport and thus solve the major problems of Tokamak reactors: size, the production of 14-megaelectron volt neutrons, and maintenance. An alternate confinement system, the field-reversed configuration, confines beams of protons and boron-11. For the proton-boron-11 fusion reaction, the fusion products are all charged particles for which direct conversion is feasible and neutron flux is negligible. 相似文献
449.
A model of three-dimensional shear wave velocity variations in the mantle reveals a tilted low velocity anomaly extending from the core-mantle boundary (CMB) region beneath the southeastern Atlantic Ocean into the upper mantle beneath eastern Africa. This anomaly suggests that Cenozoic flood basalt volcanism in the Afar region and active rifting beneath the East African Rift is linked to an extensive thermal anomaly at the CMB more than 45 degrees away. In contrast, a low velocity anomaly beneath Iceland is confined to the upper mantle. 相似文献
450.
Profiles development of the melt spinning process of poly(trimethylene terephthalate) (PTT) was simulated by a numerical method.
The spinning speed of 3 km/min to 5 km/min was analyzed and the characteristic of PTT spinning process was compared with that
of poly(ethylene terephthalate) (PET). Velocity development of PTT was slower than that of PET. Although PTT’s spinning temperature
was lower than PET’s, the PTT solidified slower because of a smaller super-cooling and the large specific heat capacity. The
diameter profile of PTT decreases gradually in comparison with that of PET. PTT’s strain rate has a broader distribution than
PET’s and its maximum ranged from 541 to 570 s−1 for PET and 136 to 149 s−1 for PTT. PTT’s tensile stress was smaller than PET’s. 相似文献