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101.
Starch-gel electrophoresis was used to resolve nine polymorphic enzyme loci from leaf tissue collected from 20 Korean populations
ofEurya japonica in order to determine differences in allele frequencies between male and female trees. In addition, 84 adults were sampled
and mapped in a population located on Naenaro Island in Korea to examine spatial genetic structure using spatial autocorrelation
analysis. Allele frequencies between males and females gave few contribution to the genetic structuring within populations.
Only nine (5%) of 180 cases were significantly different from both sexes in allele frequencies. On the other hand, Moran'sI was significantly different from the expected value in 31 (23.5%) of 132 cases. In the shortest distance (0<5m),I was significantly positive in 10 (22.7%) of 44 cases. The results indicate that a significant small scale genetic structure
was detected in the population and patch widths were inferred to be approximately 5–7 m. A nonrandom distribution of genotypes
may be indicative of restricted gene flowvia seed and pollen dispersal, and patchy establishments of genetically distinct individuals. These factors are responsible for
shaping population genetic structure ofE. japonica. 相似文献
102.
Age-related changes in calretinin-immunoreactive periglomerular cells in the rat main olfactory bulb
Hwang IK Yoo KY Nam YS Choi JH Seo K Lee IS Jung JY Kang TC Oh YS Won MH 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(5):465-469
The changes of calretinin (CR)-immunoreactive periglomerular cells in the glomerular layer of the main olfactory bulb (MOB) were investigated in rats differing ages from postnatal month 1 (PM 1) to PM 24. The number of cresyl violet-positive periglomerular cells was similar between PM 1 and PM 12, but they decreased slightly in the PM 24 group. The size of CR-immunoreactive periglomerular cells in the glomerular layer increased with age, while their numbers did not change significantly in the PM 6-PM 24 groups. In the PM 24 group, numbers of CR-positive periglomerular cell bodies and their processes decreased, while the size of CR-positive cell bodies in the glomeruli was larger than that of the previous groups. These results suggest that CR-immunoreactive periglomerular cells in the rat MOB are well-developed in the PM 6 group, and that periglomerular cells in the PM 24 group show poor CR-immunoreactivity compared to those in the PM 6 group. 相似文献
103.
Miscanthus sacchariflorus straw was used as a raw material for the manufacture of Miscanthus–wood particle composite board with Douglas-fir particles in ratios of 100/0, 80/20, 60/40, 40/60, 20/80, and 0/100. A commercial phenol–formaldehyde resin was used as a binder at 9 and 11 % for target densities of 0.50 and 0.65 g/cm3, respectively. The effects of the Miscanthus/wood particle ratio on the composite board properties were investigated. In addition, the density profile was also examined to improve the understanding of the composite board manufacturing process. Results indicate that the internal bonding value increased drastically in the board containing up to 50 % wood particles, providing a valuable parameter for subsequent research. The board properties were greatly improved with increasing density and binder addition level. 相似文献
104.
Sunaga T Oh N Hosoya K Takagi S Okumura M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(6):745-750
Canine osteoarthritis occurs frequently and causes secondary synovitis. Administration of nonsteroidal anti-inflammatory drugs (NSAIDs) is one of the major therapeutic options for pain management of joint diseases. Tepoxalin has an unique property as an NSAIDs that suppresses both cyclooxygenase and lipoxygenase. The purpose of this study was to evaluate antiproliferative effects of tepoxalin on cultured canine synovial cells. Cytotoxic effects of tepoxalin, carprofen, meloxicam and AA-861 on cultured canine synoviocytes were evaluated by MTT colorimetric assay. Apoptosis was detected by morphological observations with Giemsa or annexin V/Hoechst 33342 staining and by the inhibition of caspase-3 activity with N-Ac-Asp-Glu-Val-Asp-CHO (Ac-DEVD-CHO). Cytotoxic effects of tepoxalin were evident in comparison with the effects of carprofen or meloxicam. The same tendency of cytotoxicity was observed when 5-lipoxygenase was inhibited by AA-861. The morphological findings and contradictory effects of Ac-DEVD-CHO with regard to the cytotoxicity proved the proapoptotic effects of tepoxalin. In conclusion, tepoxalin might control osteoarthritic synovitis by inducing apoptosis in proliferating synoviocytes, while most NSAIDs that selectively inhibit cyclooxygenase-2 most likely would not suppress synovial proliferation. 相似文献
105.
Sun-Gil Do Jun-Hong Park Hajin Nam Jin-Bong Kim Jae-Yong Lee Yang-Seok Oh Jun-Gyo Suh 《Journal of veterinary science (Suw?n-si, Korea)》2012,13(4):339-344
Components of silk including silk fibroin have long been used as anti-diabetic remedies in oriental medicine. However, detailed mechanisms underlying these anti-diabetic effects remain unclear. In this study, we examined the anti-diabetic activity of silk fibroin hydrolysate (SFH) in C57BL/KsJ-db/db (db/db) mice, a well-known animal model of non-insulin dependent diabetes mellitus. When the db/db mice were administered SFH in drinking water for 6 weeks, hyperglycemia in the animals gradually disappeared and the level of glycosylated hemoglobin decreased, indicating that SFH plays important role in reducing the symptoms of diabetes. In addition, SFH-treated db/db mice exhibited improved glucose tolerance with increased plasma insulin levels. Immunohistochemical and morphological analyses showed that SFH up-regulated insulin production by increasing pancreatic β cell mass in the mice. In summary, our results suggest that SFH exerts anti-diabetic effects by increasing pancreatic β cell mass in a non-insulin dependent diabetes mellitus mouse model. 相似文献
106.
107.
M. S. Kim J. H. Kim M. R. Lee J. H. Kang H. J. Kim H. M. Ko C. H. Choi J. Y. Jung J. T. Koh B. K. Kim H. K. Oh W. J. Kim E. J. Lee S. H. Kim 《Anatomia, histologia, embryologia》2009,38(2):154-160
A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) have been reported to play a role in the degradation of aggrecan, a major component of cartilage. This study was performed to examine the effects of alendronate on the expression of ADAMTS in developing femoral epiphyseal cartilage. Primary cultured chondrocytes from this cartilage were treated with alendronate in vitro and postnatal day 1 rats were injected subcutaneously with alendronate (1 mg/kg) every second day in vivo . The number of cultured chondrocytes and their aggrecan mRNA levels were unaffected by the alendronate treatment at 10−6 to 10−4 m concentrations. The mRNA levels of ADAMTS-1, -2 and -9 in chondrocytes were also unaffected. However, the levels of ADAMTS-5 and -4 were reduced significantly by the same treatment. The thickness of the proliferating chondrocyte layers and the aggrecan mRNA levels in the epiphysis were unaffected by the alendronate treatment in vivo . However, the hypertrophied chondrocyte layers became significantly thicker, and the size of the secondary ossification centre was reduced significantly by the same treatment ( P < 0.05). Both ADAMTS-4 and -5 mRNA expressions were also reduced significantly in vivo . The immunoreactivity against ADAMTS-4 was seen in hypertrophied chondrocytes and reduced significantly by the alendronate treatment. These results suggested that alendronate can inhibit the degradation of aggrecan in the articular cartilage by downregulating the expression of matrix enzymes such as ADAMTS-4 and -5. 相似文献
108.
E Fontaine X Levy A Grellet A Luc F Bernex HJ Boulouis A Fontbonne 《Reproduction in domestic animals》2009,44(S2):196-199
There are a few investigations into endometritis in the bitch and its relationship with failure to conceive remains unclear. This may be because of the difficulty in collecting uterine samples for further investigations. Recently, transcervical catheterization by vaginal endoscopy has been introduced allowing the evaluation of the endometrium. In this study, uterine cytology and bacteriology were evaluated in 26 infertile bitches. Endometritis was bacterial in origin in most cases (70% of affected bitches), but these results may be underestimated, as some other pathogens (anaerobic bacteria, mycoplasms and fungi) were not investigated. Endometritis, in our opinion, should be investigated in each case of unexplained infertility in bitches. The method used here seems reliable although defining more accurate classification criteria will improve the efficiency of this non-invasive technique. 相似文献
109.
Measurement of transepidermal water loss from clipped and unclipped anatomical sites on the dog 总被引:1,自引:1,他引:0
Objectives To establish a standardised clipping method for the measurement of transepidermal water loss (TEWL) with a VapoMeter® in Beagle dogs and to identify the optimal anatomical site for TEWL measurement.
Procedure TEWL values obtained from skin sites on five healthy Beagles clipped using two different blade angles (standard vs non-standard) were compared. TEWL values for 48 h were also obtained from seven different anatomical sites that had differing hair density. The hair was clipped in the intensively haired anatomical sites (head, lower and upper back and tail), but not clipped in the sparsely haired sites (ear, inguinal region, footpad).
Results The TEWL values for the standard and non-standard clipping sites were 6.3 ± 1.31 and 27.2 ± 1.11 g/h/m2 , respectively. We found the upper back among the clipped sites was the most appropriate site for TEWL measurement over 48 h after clipping, whereas among the unclipped sites the ear was the most appropriate, because the TEWL values from those anatomical sites had the least fluctuation and were less affected by movement.
Conclusion The clipping method and anatomical site should be standardised in order to minimise the experimental variation in TEWL measurement in dogs. 相似文献
Procedure TEWL values obtained from skin sites on five healthy Beagles clipped using two different blade angles (standard vs non-standard) were compared. TEWL values for 48 h were also obtained from seven different anatomical sites that had differing hair density. The hair was clipped in the intensively haired anatomical sites (head, lower and upper back and tail), but not clipped in the sparsely haired sites (ear, inguinal region, footpad).
Results The TEWL values for the standard and non-standard clipping sites were 6.3 ± 1.31 and 27.2 ± 1.11 g/h/m
Conclusion The clipping method and anatomical site should be standardised in order to minimise the experimental variation in TEWL measurement in dogs. 相似文献
110.
H. T. H. Truong K. T. Kim D. W. Kim S. Kim Y. Chae J. H. Park D. G. Oh M. C. Cho 《Plant pathology》2012,61(1):48-56
Quantitative trait loci (QTL) for resistance to phytophthora root rot caused by Phytophthora capsici were investigated using two Korean P. capsici isolates and 126 F8 recombinant inbred lines derived from a cross of Capsicum annuum line YCM334 (resistant parent) and local cv. Tean (susceptible parent). The experimental design was a split plot with two replications. Highly significant effects of pathogen isolate, plant genotype, and genotype × isolate were detected. QTL mapping was performed using a genetic linkage map covering 1486·6 cM of the pepper genome, and consisted of 249 markers including 136 AFLPs (Amplified Fragment Length Polymorphisms), 112 SSRs (Simple Sequence Repeats) and one CAPS (Cleaved Amplified Polymorphic Sequence). Fifteen QTLs were detected on chromosomes 5 (P5), 10 (P10), 11 (P11), Pb and Pc using two data processing methods: percentage of wilted plants (PWP) and relative area under the disease progress curves (RAUDPC). The phenotypic variation explained by each QTL (R2) ranged from 6·0% to 48·2%. Seven QTLs were common to resistance for the two isolates on chromosome 5 (P5); six were isolate‐specific for isolate 09‐051 on chromosomes 10 (P10) and Pc, and two for isolate 07‐127 on chromosomes 11 (P11) and Pb. The QTLs in common with the major effect on the resistance for two isolates explained 20·0–48·2% of phenotypic variation. The isolate‐specific QTLs explained 6·0–17·4% of phenotypic variation. The result confirms a gene‐for‐gene relationship between C. annuum and P. capsici for root rot resistance. 相似文献