Resistenzeigenschaften im Gersten- und Weizensortiment Gatersleben 20. Prüfung des Verhaltens von Gersten gegen Flugbrand,Ustilago nuda (Jens.) Rostr.

Zusammenfassung In den Jahren 1964 bis 1974 wurden 362 Winter- und 738 Sommergersten mit einer lokalen Population vonUstilago nuda geprüft. Zur Kennzeichnung der Virulenz des Infektionsmaterials ist das Verhalten von Trägern bekannter Resistenzgene angegeben. Resistenz (0 ... 10% Befall) zeigten 197 oder 18% der 1100 Gersten. Mäßig resistent bis mäßig anfällig (>10 ... 30% Befall) waren 146 oder 13% dieses Sortiments. Als anfällig wurden 69% der Kollektion bewertet.Im Zusammenhang mit diesen Ergebnissen wird auf die Literatur zur Genetik der Flugbrandresistenz und auf Kombinationen mit anderen Resistenzmerkmalen eingegangen.

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Resistance in the Gatersleben barley and wheat collection. 20. Testing of barley for reaction to loose smut,Ustilago nuda (Jens.) Rostr.

Summary During the years 1964–74 362 winter and 738 spring barleys were inoculated with a local smut population. Its virulence is described by the reactions of varieties with identified resistance genes.Of the 1100 varieties tested 197 were resistant (0 ... 10% infection), 146 showed >10 ... 30% infection, and 69 per cent of this collection were classified as susceptible, with infection exceeding 30%.In connection with these results literature on smut resistance and the combination with resistance to other diseases are discussed.

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. 20. , Ustilago nuda (Jens.) Rostr.

1964 1974 . 362 738 Ustilago nuda. . ( 0 10%) 197 1100 (18%). ( 10 30%) 146 (13%). 69% . .

     

Evaluation of Cassia fistula meal as a replacement for soybean meal in practical diets of Oreochromis niloticus fingerlings

This study was undertaken to determine the replacement value of Cassia fistula seed meal (CFM) for soybean meal (SBM) in practical diets of Oreochromis niloticus fingerlings. Five practical diets (350 g kg^?1 crude protein) containing 0 g kg^?1 (control), 170 g kg^?1 (diet II), 340 g kg^?1 (diet III), 509 g kg^?1 (diet IV) and 670 g kg^?1 (diet V) substitution levels of CFM for SBM were formulated and fed to triplicate groups of O. niloticus fingerlings (mean initial weight of 10.22 ± 0.03 g) for 70 days. Fish mortality increased linearly with increase in inclusion levels of CFM in the diet. Growth and diet utilization efficiency were depressed in fish fed diets containing CFM at varying inclusion levels. Feed conversion ratio, specific growth rate and protein efficiency ratio of O. niloticus fed on diet containing 170 g kg^?1 substitution level of CFM were similar (P > 0.05) to the control diet. Digestibility of the different diets decreased with increase in inclusion levels of CFM. Fish fed diet containing 670 g kg^?1 CFM had significantly lower carcass protein. However, no significant differences were observed in carcass protein and lipid contents between fish fed the control diets and diet containing 170 g kg^?1 CFM. The most efficient diet in terms of cost per unit weight gain of fish was obtained in 170 g kg^?1 CFM dietary substitution.     

Growth and Yield Response of Facultative Wheat to Winter Sowing, Freezing Sowing and Spring Sowing at Different Seeding Rates

Growth and yield of wheat are affected by environmental conditions and can be regulated by sowing time and seeding rate. In this study, three sowing times [winter sowing (first week of September), freezing sowing (last week of October) and spring sowing (last week of April)] at seven seeding rates (325, 375, 425, 475, 525, 575 and 625 seeds m^?2) were investigated during the 2002–03 and 2003–04 seasons, in Erzurum (Turkey) dryland conditions, using Kirik facultative wheat. A split‐plot design was used, with sowing times as main plots and seeding rates randomized as subplots. There was a significant year × sowing time interaction for grain yield and kernels per spike. Winter‐sown wheat produced a significantly higher leaf area index, leaf area duration, spikes per square metre, kernel weight and grain yield than freezing‐ and spring‐sown wheat. The optimum time of sowing was winter for the facultative cv. Kirik. Grain yields at freezing and spring sowing were low, which was largely the result of hastened crop development and high temperatures during and after anthesis. Increasing seeding rate up to 525 seeds m^?2 increased the spikes per square metre at harvest, resulting in increased grain yield. Seeding rate, however, was not as important as sowing time in maximizing grain yield. Changes in spikes per square metre were the major contributors to the grain‐yield differences observed among sowing times and seeding rates. Yield increases from higher seeding rates were greater at freezing and spring sowing. We recommended that a seeding rate of 525 seeds m^?2 be chosen for winter sowing, and 575 seeds m^?2 for freezing and spring sowing.     

Effect of storage on measurement of ionized calcium and acid-base variables in equine, bovine, ovine, and canine venous blood.

The stability of blood ionized calcium (Ca2+) and acid-base variables in equine, bovine, ovine, and canine venous blood samples (n = 15, in each group) stored at 4 C for 3, 6, 9, 24, or 48 hours was studied. Variables included blood Ca2+ and standard ionized calcium (Ca2+ corrected to pH 7.4) concentrations, pH, blood carbon dioxide and oxygen tensions, base excess, bicarbonate concentration, and total carbon dioxide content. Results indicate that storage of blood samples at 4 C for up to 48 hours, despite appreciable acid-base changes, is associated with less than 1.5% change in equine, bovine, and ovine blood Ca2+ concentrations. Similar changes were observed in canine blood during the first 9 hours' storage. After 24 and 48 hours' storage, clinically relevant decrease (10.5 and 15.5%) in canine blood Ca2+ concentration was measured. Therefore, Ca2+ concentration in equine, bovine, and ovine venous blood samples stored up to 48 hours, and in canine blood samples stored up to 9 hours at 4 C is of diagnostic use.     

An adenovirus linked to mortality and disease in long-tailed ducks (Clangula hyemalis) in Alaska

An adenovirus was isolated from intestinal samples of two long-tailed ducks (Clangula hyemalis) collected during a die-off in the Beaufort Sea off the north coast of Alaska in 2000. The virus was not neutralized by reference antiserum against known group I, II, or III avian adenoviruses and may represent a new serotype. The prevalence of the virus was determined in live-trapped long-tailed ducks at the mortality site and at a reference site 100 km away where no mortality was observed. Prevalence of adenovirus antibodies in serum samples at the mortality site was 86% compared to 10% at the reference site. Furthermore, 50% of cloacal swabs collected at the mortality site and only 7% of swabs from the reference site were positive for adenoviruses. In 2001, no mortality was observed at either of the study areas, and virus prevalence in both serum and cloacal samples was low, providing further evidence that the adenovirus was linked to the mortality event in 2000. The virus was used to infect long-tailed ducks under experimental conditions and resulted in lesions previously described for avian adenovirus infections and similar to those observed in long-tailed duck carcasses from the Beaufort Sea. The status of long-tailed ducks has recently become a concern in Alaska due to precipitous declines in breeding populations there since the mid-1970s. Our findings suggest that the newly isolated adenovirus is a disease agent and source of mortality in long-tailed ducks, and thus could be a contributing factor in population declines.     

Study on the association of BoLA-DRB3.2 alleles with clinical mastitis in Norwegian Red cows

Genotyping of bovine leucocyte antigen DRB3.2 (BoLA-DRB3.2) in a total of 523 Norwegian Red (NR) cows from two groups selected for high protein yield and low clinical mastitis, respectively, identified 27 previously reported BoLA-DRB3.2 alleles across the groups. Significant differences in BoLA-DRB3.2 allele frequencies were found between the selection groups. Alleles *13, *18, *22 and *27 had a significantly higher frequency in cows selected for low clinical mastitis, while alleles *3, *9, *11 and *26 had a higher frequency in cows selected for high protein yield. Associations between BoLA-DRB3.2 alleles and clinical mastitis were analysed based on mastitis data from 741,072 first-lactation NR cows, of which 452 were genotyped. Alleles *22 and *26 were found to be associated with increased clinical mastitis, while alleles *7, *11, *18 and *24 had a favourable effect on mastitis resistance. Contradictory results from different studies investigating associations between BoLA-DRB3.2 alleles and mastitis indicate that future studies should focus on associations of mastitis with BoLA haplotypes rather than with single BoLA genes.     

Physical Properties of Urban Soils in Rostov Agglomeration

Transformation of particle-size composition, structure, and density of soils upon urbopedogenesis is considered for Rostov agglomeration. Various soils are compared by horizons. It is found that the share of sand fractions increases in upper and middle horizons of migration–segregation chernozems (Calcic Chernozem (Hyperhumic, Loamic)), above all, at the expense of particles of 0.05—0.001 mm in size; with the coarse medium sand fraction 1–0.25 mm being diagnostic for urbopedogenesis. The reason is the introduction of sandy particles upon urban construction, arranging water conduits and other utility lines, as well as the use of icing-control sandy mixtures. The Dolgov-Bakhtin schedule appears to be the most appropriate for assessing the structure of urban soils. Dry sieving testified to the decreasing amount of agriculturally valuable aggregates in all compared pairs of horizons in the sequence of urban soils: under forest vegetation → under steppe vegetation → in the buried massif of urbosoils. The water stability of aggregates decreases in the sequence: soils under steppe vegetation → buried horizons of urbosoils → soils under forest vegetation. The following sequence of urbic horizons (UR and RAT) shows a decrease in the share of agriculturally valuable fractions and an increase in their water stability: heavy-textured UR → light-textured UR → RAT. The density of natural soils varies insignificantly within the city territory, with its urbostratified soils (Calcic Chernozem Novic (Technic Loamic) in residential areas often manifesting the maximal density.     

Effects of Pentoxifylline on Equine Epididymal Sperm

This study evaluated whether pentoxifylline (PTX) present in the flushing extender influenced the function of equine epididymal spermatozoa after recovery and after thawing. For this experiment, 58 testicles from 29 Brazilian Jumping Horses were used. Cauda epididymides of each stallion were separated and flushed with a skim milk extender, with or without 7.18 mM PTX and then subjected to the freezing process. Samples flushed with the extender containing PTX showed a significant increase in total motility, progressive motility, straight line velocity, curvilinear velocity, and percentage of rapid sperm immediately after the recovery of epididymal sperm and after 15 minutes of incubation at 37°C (P < .05). However, the presence of PTX in the flushing extender did not affect the post-thaw motility parameters or plasma membrane integrity (P > .05). The results of this study showed that the PTX present in the flushing extender improved motility parameters of recently recovered epididymal sperm and had no deleterious effects on plasma membrane integrity and freezability of equine epididymal sperm.     

Experimental field trial with an immunostimulating complex (ISCOM) vaccine against contagious bovine pleuropneumonia

The experimental field trial with an immunostimulating complex (ISCOM) vaccine has been an occasion to explore the role of a Th1 response in the pathogenesis caused by Mycoplasma mycoides subsp. mycoides small colony (MmmSC) and in immune protection. The ISCOM complex is known to promote Th1 response. Antibodies to MmmSC were detected by indirect enzyme-linked immunosorbent assay (ELISA) in the vaccinated cattle, although the levels were lower than in a previous study. No antibodies were detected by complement fixation test (CF). After the challenge infection, vaccinated animals developed CF antibody response. They showed significantly reduced mortality compared with controls. However, gross pathological and histopathological score for vaccinated animals was as high as for the non-vaccinated, characterized by a high inflammatory reaction with histopathology dominated by interlobular pneumonia with vasculitis.