Concentration of dietary calcium supplied by calcium carbonate does not affect the apparent total tract digestibility of calcium, but decreases digestibility of phosphorus by growing pigs

A regional experiment was conducted to test the hypothesis that the concentration of dietary Ca does not affect the digestibility of Ca or P in diets fed to growing pigs. Six diets based on corn, potato protein isolate, cornstarch, and soybean oil were formulated. All diets also contained monosodium phosphate, crystalline AA, salt, and a vitamin-micromineral premix. The only difference among the diets was that varying concentrations of calcium carbonate were used to create diets containing 0.33, 0.46, 0.51, 0.67, 0.92, and 1.04% Ca. All diets contained between 0.40 and 0.43% P. Six universities participated in the experiment and each university contributed 2 replicates to the experiment for a total of 12 replicates (initial BW: 23.1 ± 4.4 kg). Pigs were placed in metabolism cages that allowed total, but separate, collection of feces and urine from the pigs. Pigs within each replicate were randomly allotted to the 6 diets and fed experimental diets for 14 d with urine and feces being collected over a 5-d period. Diets, feces, and urine samples were analyzed for Ca and P, and the daily balance, the apparent total tract digestibility (ATTD), and the retention of Ca and P were calculated. Results indicated that intake, fecal excretion, and urinary excretion of Ca increased (linear, P<0.05) as dietary Ca concentration increased. The daily intake of P was not affected by the dietary concentration of Ca, but fecal excretion of P increased (linear, P<0.05) as dietary Ca concentrations increased. In contrast, urinary P output was decreased (linear, P<0.05) as dietary Ca increased. The retention of Ca increased (linear, P<0.05) from 1.73 to 4.60 g/d, whereas the retention of P decreased (linear, P<0.05) from 1.98 to 1.77 g/d as dietary Ca concentrations increased. However, if calculated as a percentage of intake, both Ca and P retention were decreased (linear, P<0.05) as dietary Ca concentration increased (from 55.4 to 46.1% and from 48.4 to 43.5%, respectively). The ATTD of Ca was not affected by the dietary concentration of Ca, but the ATTD of P was decreased (linear, P<0.05) from 56.9 to 46.2% as dietary Ca concentration increased. It is concluded that the dietary concentration of Ca does not affect the ATTD of Ca in calcium carbonate, but increased concentrations of dietary Ca may decrease the ATTD of P in diets based on corn, potato protein isolate, and monosodium phosphate.     

Expression of a cloned rat brain potassium channel in Xenopus oocytes

Potassium channels are ubiquitous membrane proteins with essential roles in nervous tissue, but little is known about the relation between their function and their molecular structure. A complementary DNA library was made from rat hippocampus, and a complementary DNA clone (RBK-1) was isolated. The predicted sequence of the 495-amino acid protein is homologous to potassium channel proteins encoded by the Shaker locus of Drosophila and differs by only three amino acids from the expected product of a mouse clone MBK-1. Messenger RNA transcribed from RBK-1 in vitro directed the expression of potassium channels when it was injected into Xenopus oocytes. The potassium current through the expressed channels resembles both the transient (or A) and the delayed rectifier currents reported in mammalian neurons and is sensitive to both 4-aminopyridine and tetraethylammonium.     

奥地利黑松引种造林试验初步分析

在陕西周至、永寿、黄陵及延安进行了奥地利黑松引种造林试验。初步结果表明,奥地利黑松对引种地气候表现出较强的适应性,造林平均成活率达到73.7%;树高生长量与海拔、≥10℃积温和年降雨量密切相关;选用2年生容器苗在阳坡造林可提高林木生长量;4年生奥地利黑松最大平均树高和最大平均地径生长量分别超过当地对照油松15.16%和8.4%。初步认为,奥地利黑松适于在陕西秦岭以北、延安以南生长,特别在黄土高原区表现良好,值得推广。     

The effect of conditions of storage on the respiration of apples. VI. The effects of temperature and controlled atmosphere storage on the relationship between rates of production of ethylene and carbon dioxide

Studies on freshly harvested fruit of three apple cultivars kept in air or under C.A. conditions at 12°, 7.2°, 3.3° and 0 °C have shown that the initiation of ethylene production is not necessarily associated with the onset of the respiration climacteric, and therefore cast doubt on the belief that ethylene is a ripening hormone. The dissociation of the two phenomena was effected by low temperatures and by storage under C.A. conditions.

The studies showed also that there is a linear relationship between rate of production of ethylene and its concentration in the intercellular spaces of the fruit.     

The Effect of Conditions of Storage on the Respiration of Apples: III. The Effect of Modulation of Temperature on the Respiration of Cox’s Orange Pippin Apples,and on the Extent of Low Temperature Injury

When apples which develop low temperature breakdown (LTB) at 32° F. are moved from 32° F. to 65° F. for 3 to 5 days at about the 7th to 8th week of storage, they subsequently develop within a given period of storage less LTB than apples kept at 32° F. continuously.

The respiration of apples susceptible to LTB increases steadily during storage at 32° F. If these apples are warmed to 65° F. during the period of exposure to 32° F., the subsequent rate of respiration at 32° F. is lower than before warming, and continues at a lower rate than for apples kept at 32° F. continuously.

If the apples are moved to 38° F., without an intermediate treatment at 65° F., the rate of respiration is higher than for apples at 38° F. continuously, and this higher rate persists.

If there is an intermediate wanning period at 65° F., the respiration of apples moved from 32° to 38° F. is of the same order as that for apples kept at 38° F. continuously.

The respiratory quotient of apples at 32° F. or at 38° F., which is indicative of the type of respiratory activity, is typical for the temperature at which it is measured, and is not affected by the warming treatment. The effects of wanning on both the incidence of LTB and respiration are similar for apples stored in air and in 2% oxygen: 98% nitrogen.     

Vector population manipulation for control of arboviruses – a novel prospect for India

India, the seventh largest country in the world, has diverse geographical and climatic regions with vast rural and peri‐urban areas. Many are experiencing an escalation in the spread and intensity of numerous human diseases transmitted by insects. Classically, the management of these vector‐borne diseases is underpinned by either chemical insecticides and/or environmental management targeted at the vector. However, these methods or their present implementation do not offer acceptable levels of control, and more effective and sustainable options are now available. Genetic strategies for the prevention of arbovirus transmission are most advanced for dengue and chikungunya, targeting their primary vector, Aedes aegypti. The national burden in terms of morbidity and mortality as a direct consequence of dengue virus in India is considered to be the largest worldwide, over 4 times that of any other country. Presently, new genetic technologies are undergoing field evaluation of their biosafety and efficacy in several countries. This paper discusses the merits of these approaches and argues for fair and transparent appraisal in India as a matter of urgency. Identification of any associated risks and their appropriate mitigation are fundamental to that process. © 2013 Society of Chemical Industry