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排序方式: 共有163条查询结果,搜索用时 15 毫秒
61.
Patient-side assay of lipase activity correlating with pancreatic lipase immunoreactivity in the dog
Ishioka K Hayakawa N Nakamura K Terashima K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(11):1481-1483
Pancreatitis is a common exocrine pancreatic disease in dogs, and the pancreatic lipase immunoreactivity (PLI) test is used for diagnosis. Enzyme catalytic assay is thought to have low specificity, but a lipase activity assay with increased specificity has been developed in human clinical chemistry. We measured serum lipase activity of 65 client-owned dogs using the newly developed FUJI DRI-CHEM slide and compared the results with their PLI concentrations. The results showed a good correlation (r = 0.91), and the normal and pancreatitis dogs identified based on the PLI values were correctly separated based on lipase activity. The present study suggests that FUJI DRI-CHEM lipase activity would be helpful for diagnosis of pacreatitis in dogs and, in particular, that it can be used as a patient-side assay and contributes to immediate treatment. 相似文献
62.
Nagata N Yuki M Hasegawa T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(12):1585-1589
Intraocular pressure (IOP) evaluated by applanation tonometry via TONO-PEN XL (TP), and rebound tonometry via TonoVet (TV) were compared in enucleated canine eyes with varied pressure of the anterior chamber (AC) and in clinical cases. TV measured IOP values were lower than IOP measurements of TP in the enucleated eyes with 5-10 mmHg of AC (P<0.0001), though there was no significant difference in IOP values obtained with TP and TV on the pressure ranges of 15-20 mmHg. However, TP detected IOP values were lower than IOP measurements of TV in the eyes with over 25 mmHg of AC (P<0.0001). The results of clinical cases were similar to the enucleated eye model. There was no significant difference in IOP values obtained from TP and TV in dogs with normotensive eyes. IOP measurements of TP were lower than those of TV in glaucomatous eyes (P<0.0001). TV was a reliable tonometer for measurement of IOP in hypertensive eyes, whereas it was less accurate than TP in hypotensive eyes. The characteristics of TP and TV should be considered in the evaluation of IOP in practice. 相似文献
63.
Hitomi KIMURA Takako YAMAZAKI Taiki MIHARA Noriyuki KAJI Kazuhisa KISHI Masatoshi HORI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(4):610
Postoperative ileus (POI) is a postsurgical gastrointestinal motility dysfunction caused by mechanical stress to the intestine during abdominal surgery. POI leads to nausea and vomiting reduced patient quality of life, as well as high medical costs and extended hospitalization. Intestinal inflammation caused by macrophages and neutrophils is thought to be important in the mechanism of POI. Surgery-associated tissue injury and inflammation induce the release of adenosine triphosphate (ATP) from injured cells. Released ATP binds the purinergic P2X7 receptor (P2X7R) expressed on inflammatory cells, inducing the secretion of inflammatory mediators. P2X7R antagonists are thought to be important mediators of the first step in the inflammation process, and studies in chemically induced colitis models confirmed that P2X7R antagonists exhibit anti-inflammatory effects. Therefore, we hypothesized that P2X7R plays an important role in POI. POI models were generated from C57BL/6J mice. Mice were treated with P2X7R antagonist A438079 (34 mg/kg) 30 min before and 2 hr after intestinal manipulation (IM). Inflammatory cell infiltration and gastrointestinal transit were measured. A438079 ameliorated macrophage and neutrophil infiltration in the POI model. Impaired intestinal transit improved following A438079 treatment. P2X7R was expressed on both infiltrating and resident macrophages in the inflamed ileal muscle layer. The P2X7R antagonist A438079 exhibits anti-inflammatory effects via P2X7R expressed on macrophages and therefore could be a target in the treatment of POI. 相似文献
64.
Naoyuki AIHARA Noriyuki HORIUCHI Nanase HIKICHI Mariko OCHIAI Yuko HOSODA Yoko ISHIKAWA Yoko SHIMAZAKI Koji OISHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(8):913-918
Infectious bursal disease (IBD) is characterized by immunosuppression due to the depletion of lymphocytes in the atrophied bursa of Fabricius (BF). We have sometimes encountered contradictory findings: chickens infected with the vaccine IBD virus (IBDV) strain have sometimes exhibited a highly atrophied BF, but not immunosuppression. In this study, chickens administered vaccine or wild-type strains of IBDV were later vaccinated with the B1 strain of the Newcastle disease virus (NDV). Bursal changes were examined histologically with a focus on the bursal follicle. The immunoreactivity to NDV was also evaluated with the hemagglutination inhibition test. In gross examination, we observed a few chickens with a severely atrophied BF in vaccine strain-administered groups (vaccine groups), and the level of severity was the same as that in the wild-type strain-administered group (wild-type group). However, these chickens retained humoral antibody responses to NDV and were revealed to possess a higher number of bursal follicles than those of the wild-type group. These results indicated that macroscopic evaluation dose not accurately reflect the immunoreactivity and degree of bursal damage in IBDV-administered chickens. We also found non-immunosuppressed chickens in the wild-type group. These non-immunosuppressed chickens retained a significantly higher number of normal follicles and total follicles according to our statistical analysis. Furthermore, a high correlation coefficient between the NDV-HI titer and the number of normal follicles was found in the wild-type group. These results implied that the retained number of normal follicles is important for the immunoreactivity of chickens infected with IBDV. 相似文献
65.
Noriyuki HORIUCHI Makoto KOMAGATA Keiichi SHITAMURA Shiori CHIBA Kotaro MATSUMOTO Hisashi INOKUMA Takane MATSUI Yoshiyasu KOBAYASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(6):729-732
An 11-year-old Holstein-Friesian cow exhibited anorexia and jaundice. A large mass was
found in the liver during necropsy. Macroscopically, the mass was composed of dark red
multilobular tissue and a centrally located abscess, which was connected to the hepatic
duct. Histologically, the mass consisted of proliferation of small neoplastic cells and
was demarcated from the hepatic parenchyma by a thick region of granulation tissue. The
neoplastic cells were predominantly arranged in solid sheets, but they also formed
blood-filled cancellous structures, and proliferating foci were seen around blood vessels.
Periodic acid-Schiff reaction demonstrated that a fine basement membrane-like structure
surrounded the neoplastic cells. Immunohistochemically, the neoplastic cells were positive
for vimentin and alpha smooth muscle actin and negative for cytokeratin, factor
VIII-related antigen, chromogranin and desmin. Based on its histopathological features,
the hepatic neoplasm was diagnosed as a primary glomus tumor. This is the first report
about a primary glomus tumor of the liver in a cow. 相似文献
66.
Yuka KOJIMA Shiori CHIBA Noriyuki HORIUCHI Yoshiyasu KOBAYASHI Hisashi INOKUMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(5):605-607
S100B in cerebrospinal fluid (CSF-S100B) was measured in calves with 20 neurologic and 21
non-neurologic diseases to clarify its utility as a biomarker for neurologic diseases. The
median CSF-S100B value in the neurologic disease group (43.0
ng/ml) was significantly higher than that in the
non-neurologic disease group (10.2 ng/ml). As CSF-S100B
levels in calves with neurologic diseases widely differed, the utility of CSF-S100B as a
diagnostic marker for neurologic diseases in cattle remains inconclusive. 相似文献
67.
Noriyuki HORIUCHI Daishiro KUMAGAI Kotaro MATSUMOTO Hisashi INOKUMA Hidefumi FURUOKA Yoshiyasu KOBAYASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(10):1281-1283
Bovine dilated cardiomyopathy (DCM) is an autosomal recessive genetic disorder causing
congestive heart failure and subsequent death. Recently, a nonsense mutation c.343C>T
in the bovine optic atrophy 3 (OPA3) gene had been reported to cause the DCM in Holstein
cattle in Switzerland. However, the mutation has not been confirmed in bovine DCM outside
Switzerland. Nine Holstein Friesian cows that were macroscopically and histologically
diagnosed with or suspected of DCM and 12 control cows kept in Japan were tested for the
mutation. The mutation surrounding OPA3 DNA fragment was amplified by PCR and subjected to
direct sequences. The homogeneous c.343C>T mutation was proved to occur in all the
affected cows and not in the control cows. The present study is the first report of the
mutation in the DCM affected cows outside Switzerland. 相似文献
68.
Shiori CHIBA Shingo FUNATO Noriyuki HORIUCHI Kotaro MATSUMOTO Hisashi INOKUMA Hidefumi FURUOKA Yoshiyasu KOBAYASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(2):147-154
Degeneration of the optic pathway has been reported in various animal species including cattle. We experienced a case of bilateral optic tract degeneration characterized by severe gliosis in a Japanese black cattle without any obvious visual defects. To evaluate the significance, pathological nature and pathogenesis of the lesions, we examined the optic pathway in 60 cattle (41 Japanese black, 13 Holstein and 6 crossbreed) with or without ocular abnormalities. None of these animals had optic canal stenosis. Degenerative changes with severe gliosis in the optic pathway, which includes the optic nerve, optic chiasm and optic tract, were only observed in 8 Japanese black cattle with or without ocular abnormalities. Furthermore, strong immunoreactivity of glial fibrillary acidic protein was observed in the retinal stratum opticum and ganglion cell layer in all 5 cattle in which the optic pathway lesions could be examined. As etiological research, we also examined whether the
concentrations of vitamin A and vitamin B12 or bovine viral diarrhea virus (BVDV) infection was associated with optic pathway degeneration. However, our results suggested that the observed optic pathway degeneration was probably not caused by these factors. These facts indicate the presence of optic pathway degeneration characterized by severe gliosis that has never been reported in cattle without bilateral compressive lesions in the optic pathway or bilateral severe retinal atrophy. 相似文献
69.
Yamamoto Y Fukushima T Suna Y Ishii N Saeki A Seki S Tagawa S Taniguchi M Kawai T Aida T 《Science (New York, N.Y.)》2006,314(5806):1761-1764
Controlled self-assembly of a trinitrofluorenone-appended gemini-shaped amphiphilic hexabenzocoronene selectively formed nanotubes or microfibers with different photochemical properties. In these nanotubes, which are 16 nanometers in diameter and several micrometers long, a molecular layer of electron-accepting trinitrofluorenone laminates an electron-donating graphitic layer of pi-stacked hexabenzocoronene. The coaxial nanotubular structure allows photochemical generation of spatially separated charge carriers and a quick photoconductive response with a large on/off ratio greater than 10(4). In sharp contrast, the microfibers consist of a charge-transfer complex between the hexabenzocoronene and trinitrofluorenone parts and exhibit almost no photocurrent generation. 相似文献
70.
Noriyuki Miyake Hirofumi Nagai Shinro Kato Masashi Matsusaki Shiro Fukuta Reiko Takahashi Ryoji Suzuki Yasushi Ishiguro 《Journal of General Plant Pathology》2017,83(1):1-6
Two detection methods combining loop-mediated isothermal amplification (LAMP) and a bait trap were developed to detect Pythium helicoides in greenhouses containing roses, miniature roses, and poinsettias in hydroponic culture systems. In “Bait-LAMP”, a crude extract derived from perilla seeds as the bait was used in the LAMP reaction, whereas in the “Bait culture-LAMP”, a crude extract of mycelia grown out from perilla seeds onto Pythium-selective medium served as the bait. The two methods are simple and rapid for practical monitoring of P. helicoides in hydroponic culture systems. 相似文献