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排序方式: 共有163条查询结果,搜索用时 15 毫秒
41.
Shiro Fukuta Reiko Takahashi Satoru Kuroyanagi Noriyuki Miyake Hirofumi Nagai Hirofumi Suzuki Fujio Hashizume Tomoko Tsuji Hiromi Taguchi Hideki Watanabe Koji Kageyama 《European journal of plant pathology / European Foundation for Plant Pathology》2013,136(4):689-701
A loop-mediated isothermal amplification (LAMP) reaction with a primer set designed from the rDNA ITS sequence of P. aphanidermatum was developed. Results of a specificity test using 57 strains of Pythium spp. indicated that the LAMP assay gave no cross reactions in other 39 Pythium species, 11 strains of Phytophthora spp. and eight other soil borne pathogens. The detection limit was 10 fg of genomic DNA, which was ten times the sensitivity of the polymerase chain reaction. The LAMP assay was applied to hydroponic solution samples from tomato fields, and the results were compared to those of the conventional plating method. LAMP was observed to be effective for the specific detection of P. aphanidermatum. Furthermore, P. aphanidermatum was detected directly in tomato roots infected with P. aphanidermatum without DNA extraction. The LAMP method established in this study is a simple, sensitive and rapid tool for the detection of P. aphanidermatum. 相似文献
42.
43.
Hitoshi Obata Noriyuki Inoue Motoaki Matsuoka Masanao Umebayashi 《Soil Science and Plant Nutrition》2013,59(2):439-442
As a result of Cd treatment, K concentrations decreased in Cd sensItive maize and kidney bean calli (Obata et al. 1994) and in intact roots of kidney bean plants (Obata et al. unpublished). Potassium may be extruded from the roots or the absorption of K may be depressed by the Cd treatment in these Cd sensitive plants. Obata et al. (1996) observed that Cd inhibited both the efflux of H+ and influx of K+ following K+ addition in intact roots of bean. Thus Cd may affect the activity of proteins essential to ion movement., i.e. ioncarriers, channels and ATPase embedded in the membranes and/or may affect the permeability of the lipids of the membrane. 相似文献
44.
45.
Khoirun Nisa Sue Yee Lim Masayoshi Shinohara Tatsuyuki Osuga Nozomu Yokoyama Masahiro Tamura Noriyuki Nagata Kazuyoshi Sasaoka Angkhana Dermlim Rommaneeya Leela‐Arporn Tomoya Morita Noboru Sasaki Keitaro Morishita Kensuke Nakamura Hiroshi Ohta Mitsuyoshi Takiguchi 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(2):559-568
46.
We measured the protein precipitation capacity of tannins in the tissues of Quercus crispula and Quercus serrata galls induced by a cynipid (Trigonaspis sp.) to examine the nutrition hypothesis on gall induction. The protein precipitation capacity was significantly lower in
the nutritive tissues of galls, on which the cynipids feed, than in ungalled sound leaves and in the outer gall tissues. In
addition, the protein precipitation capacities in the leaves and in the outer gall tissues were significantly larger in Q. crispula than in Q. serrata samples, whereas that of the nutritive tissues did not differ between these oak species. These results suggest that gall
induction is one of the adaptations of galling cynipids to host–plant defensive compounds, and support the nutrition hypothesis. 相似文献
47.
Shizen Ohnishi Noriyuki Miyake Toru Takeuchi Fumiko Kousaka Satoshi Hiura Osamu Kanehira Miki Saito Takashi Sayama Ayako Higashi Masao Ishimoto Yoshinori Tanaka Shohei Fujita 《Breeding Science》2012,61(5):618-624
Soybean dwarf virus (SbDV) causes serious dwarfing, yellowing and sterility in soybean (Glycine max). The soybean cv. Adams is tolerant to SbDV infection in the field and exhibits antibiosis to foxglove aphid (Aulacorthum solani), which transmits SbDV. This antibiosis (termed “aphid resistance”) is required for tolerance to SbDV in the field in segregated progenies of Adams. A major quantitative trait locus, Raso1, is reported for foxglove aphid resistance. Our objectives were to fine map Raso1 and to reveal whether Raso1 alone is sufficient to confer both aphid resistance and SbDV tolerance. We introduced Raso1 into cv. Toyomusume by backcrossing and investigated the degree of aphid antibiosis to foxglove aphid and the degree of tolerance to SbDV in the field. All Raso1-introduced backcross lines showed aphid resistance. Interestingly, only one Raso1-introduced backcross line (TM-1386) showed tolerance to SbDV in the field. The results demonstrated Raso1 alone is sufficient to confer aphid resistance but insufficient for SbDV tolerance. Tolerance to SbDV was indicated to require additional gene(s) to Raso1. Additionally, Raso1 was mapped to a 63-kb interval on chromosome 3 of the Williams 82 sequence assembly (Glyma1). This interval includes a nucleotide-binding site–leucine-rich repeat encoding gene and two other genes in the Williams 82 soybean genome sequence. 相似文献
48.
Kitadai N Obi T Yamashita S Murase T Takase K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(3):395-397
Susceptibility to 13 antimicrobial agents was examined for 138 Escherichia coli isolates obtained from 192 fecal samples of wild cranes that migrated for wintering to the Izumi plain, Kagoshima prefecture in Japan. The numbers of isolates that were resistant to the antimicrobials used in this study are as follows: oxytetracycline (OTC), 22 isolates; minocycline, 7 isolates; ampicillin (ABPC), 4 isolates; nalidixic acid, 4 isolates; enrofloxacin, 2 isolates; kanamycin, one isolate. Multidrug resistant isolates exhibiting 2-4 drug resistances were obtained. All of the OTC-resistant isolates carried either the tet (A) or tet(B) gene. The bla(TEM) gene was found in all of the ABPC-resistant isolates. 相似文献
49.
Yonetomi D Kadosawa T Miyoshi K Nakao Y Homma E Hanazono K Yamada E Nakamura K Ijiri A Minegishi N Maetani S Hirayama K Taniyama H Nakade T 《Veterinary radiology & ultrasound》2012,53(4):371-380
Contrast‐enhanced magnetic resonance (MR) imaging with a new liver‐specific contrast agent gadolinium‐ethoxybenzyl‐diethylenetriamine penta‐acetic acid (Gd‐EOB‐DTPA; EOB·Primovist®) was studied in 14 normal beagles and 9 dogs with focal liver lesions. Gd‐EOB‐DTPA accumulates in normally functioning hepatocytes 20 min after injection. As with Gd‐DTPA, it is also possible to perform a dynamic multiphasic examination of the liver with Gd‐EOB‐DTPA, including an arterial phase and a portal venous phase. First, a reliable protocol was developed and the appropriate timings for the dynamic study and the parenchymal phase in normal dogs using Gd‐EOB‐DTPA were determined. Second, the patterns of these images were evaluated in patient dogs with hepatic masses. The optimal time of arterial imaging was from 15 s after injection, and the optimal time for portal venous imaging was from 40 s after injection. Meanwhile, the optimal time to observe changes during the hepatobiliary phase was from 20 min after injection. In patient dogs, 11 lesions were diagnosed as malignant tumors; all were hypointense to the surrounding normal liver parenchyma during the hepatobiliary phase. Even with a low‐field MR imaging unit, the sequences afforded images adequate to visualize the liver parenchyma and to detect tumors within an appropriate scan time. Contrast‐enhanced MR imaging with Gd‐EOB‐DTPA provides good demarcation on low‐field MR imaging for diagnosing canine focal liver lesions. 相似文献
50.
Asano M Matoba Y Ikeda T Suzuki M Asakawa M Ohtaishi N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(3):369-373
Reproduction of feral raccoons (Procyon lotor) in Hokkaido, Japan, was examined during a 2-year period by analysis of placental scars or fetuses in the uterus. Of 242 collected females, 69 (29%) were juveniles, 71 (29%) yearlings, and 102 (42%) adults. The pregnancy rate averaged 66% in yearlings and was significantly lower than the 96% average observed in adults (p<0.01). Litter size ranged from 1 to 7 offspring per female, and averaged 3.6 in yearlings and 3.9 in adults. There was no significant difference in mean litter size between yearlings and adults. In Hokkaido, the raccoon mating season peaked in February and the majority of litters were born between March and May, similar to patterns described in North America, but some females mated in summer. The reproductive potential of feral raccoons in Hokkaido was similar to that reported in North America. The recent increase in raccoon numbers can be explained by their high productivity. Harvest data suggest that hunting pressure on juveniles is lower than that for older age classes when using box traps in summer. In order to reduce the feral raccoon population, alternative hunting methods that increase juvenile mortality rates are needed. 相似文献