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91.
Mitsuo ISHII Takahiro AOKI Kazuhiro YAMAKAWA Fumie MAGATA Chikara GOJO Katsumi ITO Mitsunori KAYANO Yasuo NAMBO 《Journal of Equine Science》2013,24(2):25-29
The aim of this study was to clarify the relationship between the placental retention
time (PRT) and the reproductive performance following mating at the foal heat in
Thoroughbreds. For this purpose, we interviewed 292 farmers over a period of 3 years with
questionnaires evaluating foaling, expulsion of placenta and reproductive performance at
the foal heat in 1,432 mares. The obtained data were later compared with a previous study
of heavy draft mares. The average of the PRT of the 1,432 Thoroughbred mares was 58 ± 88
min (mean ± SD). The mean PRT of Thoroughbreds was significantly shorter than that of
the148 min of heavy draft mares. The incidences of retained placenta (RP) occurring in the
Thoroughbred mares were 5.2 and 4.0%, for over 3 and 4 hr after foaling, respectively. The
incidence of RP over 4 hr was significantly lower than that of 25% in heavy draft mares.
The pregnancy rate at foal heat of the mares in which PRT was less than 3 hr was 37%, and
it significantly decreased to 11% for those with PRT of more than 3 hr. In the comparison
of the reproductive performance between Thoroughbred and heavy draft mares, the pregnancy
rate of Thoroughbreds dropped drastically to 10% when PRT exceeded 40, and in consequence,
the pregnancy rate of Thoroughbreds was significantly lower than the 30% of heavy draft
mares, which had a PRT of over 4 hr. In conclusion, the Thoroughbred mares had a low
incidence of RP, however, a PRT exceeding 3 hr severely affected the reproductive
performance at the foal heat. 相似文献
92.
Norihito Yamauchi Jyuichi Shimazu Mamoru Satou Seizo Horiuchi Takashi Shirakawa 《Journal of General Plant Pathology》2004,70(6):308-313
Races were identified among butterhead lettuce isolates of Fusarium oxysporum f. sp. lactucae collected from three geographical areas of Hokkaido, Shizuoka, and Fukuoka in Japan by inoculation tests using Fujinagas race differential cultivars of lettuce (i.e., Patriot, Costa Rica No. 4, and Banchu Red Fire). Eighteen isolates from Shizuoka and Fukuoka were designated race 3, with two unknown vegetative compatibility groups (VCGs) that differed from Ogisos VCG 1 and 2. These two new VCGs were obtained from both Shizuoka and Fukuoka. On the other hand, three isolates from Hokkaido were classified as race 1 and identified as VCG 1, which represents a VCG of crisphead isolates from Nagano. 相似文献
93.
Isolation of genes showing increased expression during bovine adipocyte differentiation 总被引:1,自引:0,他引:1
Tadashi YAMASAKI Miho INOUE-MURAYAMA Koji TAHARA Shoichi TAKANO Akinori SUGIYAMA Tomohito ITOH Akiko TAKASUGA Yoshikazu SUGIMOTO Michael T. ROZE Hisashi ASO Shin'ichi ITO 《Animal Science Journal》2005,76(5):479-489
The adipocyte is important not only for the storage of excess energy as fat, but also for the secretion of homeostatic factors. Gene expression profiles during adipocyte differentiation have been reported previously for mouse 3T3‐L1 cells. However, the profiles of adipogenic gene expression in mice and cattle may be different because several metabolic pathways of the ruminant adipose tissue are different from those of non‐ruminants. The gene expression profile in a clonal bovine intramuscular preadipocyte cell line during adipogenesis was examined using the polymerase chain reaction‐subtraction method. Six hundred and twenty‐one clones, which were expressed at an early stage of differentiation, from the preadipocyte to adipocyte, were isolated and characterized. Further detailed studies were carried out for 86 selected genes using northern blotting. Ten genes were found to be highly expressed after differentiation of bovine intramuscular preadipocyte cells. In particular, the expression profiles of genes for stearoyl CoA desaturase and FK506 binding protein were quite different from the time course of differentiation of that seen in the 3T3‐L1 cells reported previously. In addition, these genes were assigned to bovine chromosomes using a bovine/hamster somatic cell hybrid panel and public database. 相似文献
94.
Norihito YAMAUCHI Seizo HORIUCHI Mamoru SATOU 《Journal of General Plant Pathology》2001,67(4):288-290
Isolates of Fusarium oxysporum f. sp. lactucae obtained from six localities in Japan were divided into three patho-genicity groups. Group 1 was highly pathogenic to lettuce
cultivars of crisphead and red leaf types and was less pathogenic to butterhead and green leaf type cultivars. Group 2 was
highly pathogenic to butterhead type and less pathogenic to crisphead and leaf types. Group 3 was less pathogenic to all lettuce
types than groups 1 and 2. These results indicated pathogenic differentiation in F. oxysporum f. sp. lactucae, roughly relating to horticultural types of host lettuce cultivars.
Received 21 February 2001/ Accepted in revised form 28 May 2001 相似文献
95.
The smothering effect of four legume covers, Canavalia ensiformis , Lablab purpureus, Mucuna pruriens and Calopogonium caeruleum , on Pennisetum polystachion ssp. setosum , a troublesome weed in rubber plantations in southern Thailand, was evaluated. Growing legume covers with Pennisetum , M. pruriens , C. ensiformis and L. purpureus caused strong suppression of Pennisetum in this order. Pennisetum grew little under 10% relative light intensity (RLI), but almost normally under 40% RLI. The three legume species generally attained lower RLI than C. caeruleum . The high shading ability of these three species was thought to contribute to the suppression of Pennisetum growth. 相似文献
96.
Md. Tofazzal ISLAM Toshiaki ITO Satoshi TAHARA 《Journal of General Plant Pathology》2002,68(2):111-117
The mode of aggregation, attachment and differentiation of zoospores of the phytopathogenic fungus Aphanomyces cochlioides when interacting with the host and a host-specific attractant and a G-protein activator, mastoparan, was studied by light
and scanning electron microscopy. When a zoospore approached very close to the host root, it seemed to halt, then coiled its
anterior flagellum on its body. The halted zoospore appeared to contact the host surface with its posterior flagellum, which
gradually drew the encysting zoospore onto the root surface. The spore then docked precisely on the root surface at its ventral
face with the help of the posterior flagellum and anchored itself by releasing some adhesive materials. The adherent spore
became a spherical after shedding its flagella and rapidly turned into an expanded cyst forming a smooth cyst coat around
it, and finally changed into a smaller cystospore covered with a wrinkled surface. In contrast, the mastoparan- or cochliophilin
A-stimulated zoospores on artificial membranes aggregated by using their posterior flagella before encystment. These contrasting
phenomena suggest that A. cochlioides zoospores may use their posterior flagella for successful docking on the host surface or for aggregation of encysting spores
in the absence of the host.
Received 30 August 2001/ Accepted in revised form 8 November 2001 相似文献
97.
Kyung-Won HONG Miho INOUE-MURAYAMA Akihiro NAKAMURA Kenji NAGAO Shin'ichi ITO 《Animal Science Journal》2008,79(5):641-643
We surveyed two polymorphic microsatellites in a candidate gene for fear response, monoamine oxidase A ( MAOA ), in chicken. Two hundred and eighty chickens from five breeds (Nagoya, Mikawa, White Leghorn, White Plymouth Rock, and Rhode Island Red) were investigated. A thymine (T) repeat and an adenine (A) repeat were found on MAOA intron 4 ( CMin4T ) and intron 9 ( CMin9A ), respectively. Nine alleles ( 127-bp to 166-bp ) in CMin4T and six alleles ( 184-bp to 198-bp ) in CMin9A were detected. A 128-bp allele in CMin4T was observed in the Nagoya breed only, implying a highly useful marker for discriminating the Nagoya breed from other breeds. In addition, the Mikawa breed had the fixed 127-bp and 198-bp alleles in CMin4T and CMin9A , respectively. The Nagoya breed chickens show densely cowardly behavior, but the Mikawa breed chickens do not show the same behavior. The current results may indicate that MAOA is an informative candidate gene for breed difference. 相似文献
98.
Effects of serotonin injected into the third ventricle on prolactin and growth hormone secretion in Holstein steers 总被引:1,自引:1,他引:0
Etsuko KASUYA Shiro KUSHIBIKI Ken-ichi YAYOU Shuichi ITO Toshiyuki SAITO Koichi HODATE Madoka SUTOH 《Animal Science Journal》2008,79(3):362-367
In order to clarify the role of serotonin (5-HT) in the regulation of pituitary hormones, the effects of 5-HT injected into the third ventricle (3V) on prolactin (PRL) and growth hormone (GH) release were investigated in Holstein steers. A chronic cannula was implanted in 3V by stereotaxic surgery under general anesthesia. After sufficient recovery from surgery, 5-HT (0, 0.1, 1.0, 2.0 mg) was injected into via the cannula and blood samples were collected over 4 h. Plasma PRL and GH concentrations were determined by radioimmunoassay. PRL release was significantly stimulated by the injection of 5-HT. The increase in PRL was observed at 20 min after the injection at three doses and the highest dose (2.0 mg) was the most effective in stimulating PRL release. The injection of 5-HT into 3V, at all doses tested, did not alter GH release significantly. Our results suggest that 5-HT is involved in the regulation of PRL release partly through the hypothalamus in cattle. 相似文献
99.
Erika HAYASHI Sayaka WAKAYAMA Daiyu ITO Ayumi HASEGAWA Keiji MOCHIDA Masatoshi OOGA Atsuo OGURA Teruhiko WAKAYAMA 《The Journal of reproduction and development》2022,68(2):118
Mammalian embryos are most commonly cryopreserved in liquid nitrogen; however, liquid nitrogen is not available in special environments, such as the International Space Station (ISS), and vitrified embryos must be stored at −80°C. Recently, the high osmolarity vitrification (HOV) method was developed to cryopreserve mouse 2-cell stage embryos at −80°C; however, the appropriate embryo is currently unknown. In this study, we compared the vitrification resistance of in vivo-derived, in vitro fertilization (IVF)-derived, and intracytoplasmic sperm injection (ICSI)-derived mouse 2-cell embryos against cryopreservation at −80°C. The ICSI embryos had lower survival rates after warming and significantly lower developmental rates than the in vivo and IVF embryos. Further, IVF embryos had a lower survival rate after warming, but a similar rate to the in vivo embryos to full-term development. This result was confirmed by simultaneous vitrification of in vivo and IVF embryos in the same cryotube using identifiable green fluorescent protein-expressing embryos. We also evaluated the collection timing of the in vivo embryos from the oviduct and found that late 2-cell embryos had higher survival and developmental rates to full-term than early 2-cell embryos. Some early 2-cell embryos remained in the S-phase, whereas most late 2-cell embryos were in the G2-phase, which may have affected the tolerance to embryo vitrification. In conclusion, when embryos must be cryopreserved under restricted conditions, such as the ISS, in vivo fertilized embryos collected at the late 2-cell stage without long culture should be employed. 相似文献
100.