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101.
Go SEHATA Hiroaki SATO Toshihiro ITO Yoshitaka IMAIZUMI Taichi NORO Eiji OISHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(7):851-855
We used real-time RT-PCR and virus titration to examine canine distemper virus
(CDV) kinetics in peripheral blood and rectal and nasal secretions from 12 experimentally
infected dogs. Real-time RT-PCR proved extremely sensitive, and the correlation between
the two methods for rectal and nasal (r=0.78, 0.80) samples on the peak day of viral RNA
was good. Although the dogs showed diverse symptoms, viral RNA kinetics were similar; the
peak of viral RNA in the symptomatic dogs was consistent with the onset of symptoms. These
results indicate that real-time RT-PCR is sufficiently sensitive to monitor CDV
replication in experimentally infected dogs regardless of the degree of clinical
manifestation and suggest that the peak of viral RNA reflects active CDV replication. 相似文献
102.
Takuma AOKI Hiroshi SUNAHARA Keisuke SUGIMOTO Tetsuro ITO Eiichi KANAI Yoko FUJII 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(4):487-491
Case 1 involved a 4-month-old intact male Somali cat in which peripheral pulmonary artery
stenosis (PPS) was recognized after a cardiac murmur remained following patent ductus
arteriosus ligation. Case 2, which involved a 1-year-old neutered male Norwegian Forest
cat, and Case 3, which involved a 6-month-old intact female American Curl cat, were
referred, because of cardiac murmurs. Grades III to IV/VI systolic heart murmurs were
auscultated at the left heart base in all 3 cats. All cases showed bilateral pulmonary
artery stenosis, although there were no associated clinical signs. In Cases 1 and 2, the
pressure gradient through the stenosis decreased after treatment with atenolol. 相似文献
103.
Takuma AOKI Hiroshi SUNAHARA Keisuke SUGIMOTO Tetsuro ITO Eiichi KANAI Yoko FUJII 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(3):331-336
Infective endocarditis (IE) in dogs with cardiac shunts has not been reported previously.
However, we encountered a dog with concurrent patent ductus arteriosus (PDA) and IE. The
dog was a 1-year-old, 13.9-kg female Border collie and presented with anorexia, weight
loss, pyrexia (40.4°C) and lameness. A continuous murmur with maximal intensity over the
left heart base (Levine 5/6) was detected on auscultation. Echocardiography revealed a PDA
and severe aortic stenosis (AS) caused by aortic-valve vegetative lesions.
Corynebacterium spp. and Bacillus subtilis were
isolated from blood cultures. The dog responded to aggressive antibiotic therapy, and the
PDA was subsequently surgically corrected. After a series of treatments, the dog showed
long-term improvement in clinical status. 相似文献
104.
Takeshi TSUKA Tomokazu KOZU Yuji SUNDEN Takehito MORITA Yoshiharu OKAMOTO Masamichi YAMASHITA Tomohiro OSAKI Takao AMAHA Norihiko ITO Yusuke MURAHATA Tomohiro IMAGAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(3):373
A 21-year-old female spotted seal (Phoca largha), with a swollen abdomen, had a five-month history of anorexia and vomiting. Ultrasonography revealed an extended mass with central necrotic foci in the right cranial abdomen. Computed tomography revealed an abdominal mass with a low-density central lumen and a pulmonary nodular lesion. Cytology of an abdominal specimen collected through fine-needle aspiration indicated a malignant tumor with round, atypical cells with large nuclei. Three days after diagnosis, necropsy revealed a 10-cm large, solid, whitish mass in the pancreatic parenchyma and multiple small nodules in the liver, spleen, mesentery, lungs, and mediastinal lymph nodes. Histopathological analysis showed prolific neoplastic cells with marked atypia and occasional keratinization. Immunohistochemistry revealed that the neoplastic cells were positive for cytokeratin AE1/AE3 antibody. Thus, the seal was diagnosed with squamous cell carcinoma, of presumed pancreatic origin, which had metastasized to multiple organs. 相似文献
105.
Yoshiki Itoh Seiya Maehara Norihiko Itoh Kazuto Yamashita Yasuharu Izumisawa 《Journal of veterinary science (Suw?n-si, Korea)》2013,14(1):77-84
Electroretinography (ERG) is a well-established diagnostic procedure for objectively evaluating retinal function. In this study, ERG in beagle dogs, which are a popular experimental animal, was performed to determine the normal range of ERG variables and assess differences between the left and right eyes. ERG findings including rod, combined rod-cone, single-flash cone, and 30-Hz flicker responses were recorded with an LED-electrode in 43 sedated beagle dogs. The subjects were divided into young (< 1 year old), adult (1~5 years old), and senile animals (≥ 6 years old). Normal ERG ranges were obtained. Significant differences in b-wave amplitude along with b/a ratio of the combined rod-cone response were found between the young and adult animals as well as young and senile dogs. No significant differences were observed between the left and right eyes. ERG variables in beagle dogs differed by age due to age-related retinal changes. Thus, we propose that normal ERG ranges should be determined according to age in each clinic and laboratory using its own equipment because each institution usually has different systems or protocols for ERG testing. 相似文献
106.
Mitsuo ISHII Takahiro AOKI Kazuhiro YAMAKAWA Fumie MAGATA Chikara GOJO Katsumi ITO Mitsunori KAYANO Yasuo NAMBO 《Journal of Equine Science》2013,24(2):25-29
The aim of this study was to clarify the relationship between the placental retention
time (PRT) and the reproductive performance following mating at the foal heat in
Thoroughbreds. For this purpose, we interviewed 292 farmers over a period of 3 years with
questionnaires evaluating foaling, expulsion of placenta and reproductive performance at
the foal heat in 1,432 mares. The obtained data were later compared with a previous study
of heavy draft mares. The average of the PRT of the 1,432 Thoroughbred mares was 58 ± 88
min (mean ± SD). The mean PRT of Thoroughbreds was significantly shorter than that of
the148 min of heavy draft mares. The incidences of retained placenta (RP) occurring in the
Thoroughbred mares were 5.2 and 4.0%, for over 3 and 4 hr after foaling, respectively. The
incidence of RP over 4 hr was significantly lower than that of 25% in heavy draft mares.
The pregnancy rate at foal heat of the mares in which PRT was less than 3 hr was 37%, and
it significantly decreased to 11% for those with PRT of more than 3 hr. In the comparison
of the reproductive performance between Thoroughbred and heavy draft mares, the pregnancy
rate of Thoroughbreds dropped drastically to 10% when PRT exceeded 40, and in consequence,
the pregnancy rate of Thoroughbreds was significantly lower than the 30% of heavy draft
mares, which had a PRT of over 4 hr. In conclusion, the Thoroughbred mares had a low
incidence of RP, however, a PRT exceeding 3 hr severely affected the reproductive
performance at the foal heat. 相似文献
107.
108.
MASAYUKI IWAHASHI YUTAKA ISODA SHIN-ICHI ITO YOSHIOKI OOZEKI SATOSHI SUYAMA 《Fisheries Oceanography》2006,15(2):125-138
Since there have been practically no surveys of the eggs of Pacific saury (Cololabis saira) in the western North Pacific (WNP), its spawning ground (SG) distribution has been poorly resolved, based mainly on the larval distribution. This means of estimating SG distribution is imprecise because saury eggs drift for more than a week before they hatch, in a region with intense western boundary currents and their extensions. To improve our understanding of the immature saury, a large number of larvae (body length <25 mm) collected in the WNP during 1993–96 were numerically backtracked to take into account the advection by geostrophic and wind‐forced Ekman currents, and the SG locations and ambient sea surface temperatures (SSTs) for the eggs and larvae on the backtracking trajectories were estimated. The resulting seasonal distributions of SGs indicated that both the locations and the intensities of spawning change from season to season. Moreover, the ambient SSTs for eggs just after fertilization ranged from a high of around 21.5°C in early autumn (September to October) to a low of around 15.0°C in late spring (May to June) with an intermediate of around 20.0°C in winter (January to February). The ambient SSTs showed seasonally different gradients while the individuals developed from eggs to early larvae: the SSTs decreased throughout the autumn (September to December), stayed rather constant in winter (January to February), and increased throughout the spring (March to June). The ambient SSTs for the early larvae were at around 19.0°C in autumn and winter (September to February) and around 16.5°C throughout the spring (March to June). 相似文献
109.
Pramod DHAKAL Nobuo TSUNODA Rie NAKAI Tomoki KITAURA Takehiro HARADA Masahiro ITO Kentaro NAGAOKA Yuko TOISHI Hiroyuki TANIYAMA Watanabe GEN Kazuyoshi TAYA 《Journal of Equine Science》2011,22(2):29-36
Changes in follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin,
immunoreactive(ir)-inhibin, testosterone, estradiol-17β, and insulin-like growth factor
(IGF)-I in Thoroughbred stallions along with changes in prolactin secretion in geldings
were studied. The correlations of day-length with changes in the concentrations of these
hormones were also studied. Five stallions and thirteen geldings were employed to draw
blood samples in monthly basis and radioimmunoassay was performed to measure these
hormones. All hormones showed a seasonal pattern, the levels being highest during the
breeding season and lowest during the winter months. Most of the hormones were at their
highest concentration during the month of April, the mid of spring in northern hemisphere.
The concentration of circulating IGF-I also demonstrated seasonality, the peak lying on
the month of April. The plasma concentration of prolactin also increased during the
breeding season. This phenomenon was similar both in stallions and geldings although
geldings had lower concentration than that of stallions. The changes in concentration of
prolactin in stallions and geldings correlated more towards the day-length than towards
the temperature. These results clearly indicate the seasonality of pituitary and gonadal
hormones of Thoroughbred stallions, the activity being highest during the month of April
and May of the breeding season. 相似文献