全文获取类型
收费全文 | 1287篇 |
免费 | 38篇 |
国内免费 | 1篇 |
专业分类
林业 | 128篇 |
农学 | 45篇 |
基础科学 | 1篇 |
232篇 | |
综合类 | 101篇 |
农作物 | 41篇 |
水产渔业 | 189篇 |
畜牧兽医 | 436篇 |
园艺 | 32篇 |
植物保护 | 121篇 |
出版年
2023年 | 7篇 |
2022年 | 16篇 |
2021年 | 18篇 |
2020年 | 14篇 |
2019年 | 20篇 |
2018年 | 17篇 |
2017年 | 25篇 |
2016年 | 30篇 |
2015年 | 20篇 |
2014年 | 48篇 |
2013年 | 113篇 |
2012年 | 69篇 |
2011年 | 76篇 |
2010年 | 46篇 |
2009年 | 57篇 |
2008年 | 76篇 |
2007年 | 69篇 |
2006年 | 68篇 |
2005年 | 75篇 |
2004年 | 56篇 |
2003年 | 61篇 |
2002年 | 31篇 |
2001年 | 46篇 |
2000年 | 50篇 |
1999年 | 32篇 |
1998年 | 16篇 |
1997年 | 5篇 |
1996年 | 4篇 |
1995年 | 6篇 |
1993年 | 11篇 |
1992年 | 7篇 |
1991年 | 15篇 |
1990年 | 15篇 |
1989年 | 6篇 |
1988年 | 11篇 |
1987年 | 9篇 |
1986年 | 6篇 |
1985年 | 7篇 |
1984年 | 4篇 |
1983年 | 7篇 |
1982年 | 8篇 |
1981年 | 5篇 |
1980年 | 3篇 |
1979年 | 8篇 |
1978年 | 4篇 |
1977年 | 3篇 |
1976年 | 5篇 |
1974年 | 4篇 |
1972年 | 3篇 |
1968年 | 4篇 |
排序方式: 共有1326条查询结果,搜索用时 171 毫秒
101.
Kobayashi N Fujiwara T Westerman KA Inoue Y Sakaguchi M Noguchi H Miyazaki M Cai J Tanaka N Fox IJ Leboulch P 《Science (New York, N.Y.)》2000,287(5456):1258-1262
Because of a critical shortage in suitable organs, many patients with terminal liver disease die each year before liver transplantation can be performed. Transplantation of isolated hepatocytes has been proposed for the temporary metabolic support of patients awaiting liver transplantation or spontaneous reversion of their liver disease. A major limitation of this form of therapy is the present inability to isolate an adequate number of transplantable hepatocytes. A highly differentiated cell line, NKNT-3, was generated by retroviral transfer in normal primary adult human hepatocytes of an immortalizing gene that can be subsequently and completely excised by Cre/Lox site-specific recombination. When transplanted into the spleen of rats under transient immunosuppression, reversibly immortalized NKNT-3 cells provided life-saving metabolic support during acute liver failure induced by 90% hepatectomy. 相似文献
102.
Tumor immunity produced by the intradermal inoculation of living tumor cells and living Mycobacterium bovis (strain BCG) 总被引:9,自引:0,他引:9
The intradermal inoculation of mixtures containing living tumor cells and living Mycobacterium bovis (strain BCG) into unimmunized syngeneic guinea pigs results in an inflammatory reaction to the BCG, and there is no progressive tumor growth. In the absence of BCG the tumor grows progressively, metastasizes, and kills the animal. By conventional methods, it has not been possible to immunize syngeneic guinea pigs to the tumor used. Guinea pigs that receive mixtures of BCG and tumor cells, however, develop specific systemic tumor immunity as measured by delayed cutaneous hypersensitivity and by suppression of tumor growth. 相似文献
103.
Multiple principal sigma factor homologs in eubacteria: identification of the "rpoD box" 总被引:23,自引:0,他引:23
Genes for the principal sigma factor (rpoD genes) of various eubacteria were identified with a synthetic oligonucleotide probe corresponding to a conserved sequence in rpoD gene products of Escherichia coli and Bacillus subtilis. Multiple rpoD homologs were found in the strains of Micrococcus, Pseudomonas, and Streptomyces, whereas single genes were detected in E. coli, B. subtilis, and Staphylococcus aureus. The four rpoD homologs of Streptomyces coelicolor A3(2) were cloned and sequenced. A homologous portion with 13 amino acids was found in the rpoD genes of S. coelicolor A3(2), E. coli, and B. subtilis and was named the "rpoD box." 相似文献
104.
Rats maintained on a low phosphorus diet supplemented with 25-hydroxyvitamin D(3) show high intestinal calcium transport activity as compared to rats similarly treated but fed a diet containing adequate phosphorus. This increased transport activity is correlated with an increased biosynthesis of 1,25-dihydroxyvitamin D(3), the probable metabolically active form of the vitamin in the intestine. 相似文献
105.
Wahlen M Tanaka N Henry R Deck B Zeglen J Vogel JS Southon J Shemesh A Fairbanks R Broecker W 《Science (New York, N.Y.)》1989,245(4915):286-290
Measurements of carbon-14 in small samples of methane from major biogenic sources, from biomass burning, and in "clean air" samples from both the Northern and Southern hemispheres reveal that methane from ruminants contains contemporary carbon, whereas that from wetlands, pat bogs, rice fields, and tundra is somewhat, depleted in carbon-14. Atmospheric (14)GH(4) seems to have increased from 1986 to 1987, and levels at the end of 1987 were 123.3 +/- 0.8 percent modern carbon (pMC) in the Northern Hemisphere and 120.0 +/- 0.7 pMC in the Southern Hemisphere. Model calculations of source partitioning based on the carbon-14 data, CH(4) concentrations, and delta(13)C in CH(4) indicate that 21 +/- 3% of atmospheric CH(4) was derived from fossil carbon at the end of 1987. The data also indicate that pressurized water reactors are an increasingly important source of (14)CH(4). 相似文献
106.
Reversal of oncogenesis by the expression of a major histocompatibility complex class I gene 总被引:26,自引:0,他引:26
The classical transplantation antigens (the major histocompatibility complex class I antigens) play a key role in host defense against cells expressing foreign antigens. Several naturally occurring tumors and virally transformed cells show an overall suppression of these surface antigens. Since the class I molecules are required in the presentation of neoantigens on tumor cells to the cytotoxic T lymphocytes, their absence from the cell surface may lead to the escape of these tumors from immunosurveillance. To test this possibility, a functional class I gene was transfected into human adenovirus 12-transformed mouse cells that do not express detectable levels of class I antigens; the transformants were tested for expression of the transfected gene and for changes in oncogenicity. The expression of a single class I gene, introduced by DNA-mediated gene transfer into highly tumorigenic adenovirus 12-transformed cells, was sufficient to abrogate the oncogenicity of these cells. This finding has important implications for the regulation of the malignant phenotype in certain tumors and for the potential modulation of oncogenicity through derepression of the endogenous class I genes. 相似文献
107.
Earth tides can trigger shallow thrust fault earthquakes 总被引:6,自引:0,他引:6
We show a correlation between the occurrence of shallow thrust earthquakes and the occurrence of the strongest tides. The rate of earthquakes varies from the background rate by a factor of 3 with the tidal stress. The highest correlation is found when we assume a coefficient of friction of mu = 0.4 for the crust, although we see good correlation for mu between 0.2 and 0.6. Our results quantify the effect of applied stress on earthquake triggering, a key factor in understanding earthquake nucleation and cascades whereby one earthquake triggers others. 相似文献
108.
Hanayama R Tanaka M Miyasaka K Aozasa K Koike M Uchiyama Y Nagata S 《Science (New York, N.Y.)》2004,304(5674):1147-1150
Apoptotic cells expose phosphatidylserine and are swiftly engulfed by macrophages. Milk fat globule epidermal growth factor (EGF) factor 8 (MFG-E8) is a protein that binds to apoptotic cells by recognizing phosphatidylserine and that enhances the engulfment of apoptotic cells by macrophages. We report that tingible body macrophages in the germinal centers of the spleen and lymph nodes strongly express MFG-E8. Many apoptotic lymphocytes were found on the MFG-E8-/- tingible body macrophages, but they were not efficiently engulfed. The MFG-E8-/- mice developed splenomegaly, with the formation of numerous germinal centers, and suffered from glomerulonephritis as a result of autoantibody production. These data demonstrate that MFG-E8 has a critical role in removing apoptotic B cells in the germinal centers and that its failure can lead to autoimmune diseases. 相似文献
109.
Rice Full-Length cDNA Consortium;National Institute of Agrobiological Sciences Rice Full-Length cDNA Project Team Kikuchi S Satoh K Nagata T Kawagashira N Doi K Kishimoto N Yazaki J Ishikawa M Yamada H Ooka H Hotta I Kojima K Namiki T Ohneda E Yahagi W Suzuki K Li CJ Ohtsuki K Shishiki T;Foundation of Advancement of International Science Genome Sequencing & Analysis Group Otomo Y Murakami K Iida Y Sugano S Fujimura T Suzuki Y Tsunoda Y Kurosaki T Kodama T Masuda H Kobayashi M Xie Q Lu M 《Science (New York, N.Y.)》2003,301(5631):376-379
110.
Oda E Ohki R Murasawa H Nemoto J Shibue T Yamashita T Tokino T Taniguchi T Tanaka N 《Science (New York, N.Y.)》2000,288(5468):1053-1058
A critical function of tumor suppressor p53 is the induction of apoptosis in cells exposed to noxious stresses. We report a previously unidentified pro-apoptotic gene, Noxa. Expression of Noxa induction in primary mouse cells exposed to x-ray irradiation was dependent on p53. Noxa encodes a Bcl-2 homology 3 (BH3)-only member of the Bcl-2 family of proteins; this member contains the BH3 region but not other BH domains. When ectopically expressed, Noxa underwent BH3 motif-dependent localization to mitochondria and interacted with anti-apoptotic Bcl-2 family members, resulting in the activation of caspase-9. We also demonstrate that blocking the endogenous Noxa induction results in the suppression of apoptosis. Noxa may thus represent a mediator of p53-dependent apoptosis. 相似文献