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排序方式: 共有532条查询结果,搜索用时 15 毫秒
81.
82.
Saiga A Iwai K Hayakawa T Takahata Y Kitamura S Nishimura T Morimatsu F 《Journal of agricultural and food chemistry》2008,56(20):9586-9591
In this study, collagen extracted from chicken legs (which are the yellow keratin parts containing a nail) was hydrolyzed with various enzymes, and the angiotensin I-converting enzyme (ACE)-inhibitory activity of each hydrolysate was determined. The hydrolysate by treatment with an Aspergillus species-derived enzyme had the highest activity (IC 50 = 260 microg/mL). The fraction of this hydrolysate obtained by ultrafiltration with a molecular-weight cutoff of 3000 Da (low fraction) had a stronger activity (IC 50 = 130 microg/mL) than the fractionated one. This fraction was further fractionated by HPLC, and the peptides in the fraction with high ACE-inhibitory activity were identified. The amino acid sequences of the four peptides were identified using a protein sequencer. These peptides were synthesized to confirm their ACE-inhibitory activities; this showed that peptides with a Gly-Ala-Hyp-Gly-Leu-Hyp-Gly-Pro sequence had the highest activity (IC 50 = 29 microM). When the low fraction was administered to spontaneous hypertensive rats, a decrease in their blood pressure was observed after 2 h of administration, and a significant decrease in blood pressure (-50 mmHg) was observed after 6 h. Moreover, long-term administration studies indicated that the low fraction showed a significant suppression of increased blood pressure. 相似文献
83.
Comparative aspects on the role of polypyrimidine tract-binding protein in internal initiation of hepatitis C virus and picornavirus RNAs 总被引:1,自引:0,他引:1
Nishimura T Saito M Takano T Nomoto A Kohara M Tsukiyama-Kohara K 《Comparative immunology, microbiology and infectious diseases》2008,31(5):435-448
We compared the effects of polypyrimidine tract-binding protein (PTB) on hepatitis C virus (HCV genotype IIa), encephalomyocarditis virus (EMCV) and poliovirus internal ribosome entry site (IRES) activities in vitro. It bound strongly to EMCV IRES, but weakly to PV and HCV RNAs. PV IRES showed the strongest dependency to PTB and it showed less than one-tenth of IRES activity after the immuno-depletion of PTB from HeLa S10 lysate with pre-coated anti-PTB IgG beads, comparing to the normal IgG beads-treated S10 lysate. EMCV IRES activity was approximately 40% of that of normal control after PTB depletion. Especially, HCV IRES activity was approximately 95%, and most weekly affected by the depletion of PTB. Repletion of PTB to depleted S10 lysate restored activities of PV and EMCV IRESs. The data suggest that PTB plays an important role in picornaviral IRESs, but not in HCV IRES. 相似文献
84.
Ohmori K Kawarai S Yasuda N Tanaka A Matsuda H Nishimura R Sasaki N Tsujimoto H Masuda K 《Veterinary immunology and immunopathology》2008,126(1-2):43-53
Gain-of-function mutations in the proto-oncogene c-kit have been considered the molecular mechanism of neoplastic proliferation of mast cells. However, the importance of c-kit gene mutations is not well evaluated in canine mast cell tumors (MCTs). In the present study, we established and characterized a mast cell line, HRMC, derived from a dog with MCT. We also examined c-kit mutations in HRMC cells and assessed an inhibitory effect of a tyrosine kinase inhibitor, STI571, on HRMC cells. HRMC cells had cytoplasmic metachromatic granules, chymase and tryptase, and expressed both KIT and FcepsilonRI on the cell surface. HRMC cells contained histamine and released beta-hexosaminidase through FcepsilonRI cross-linking and calcium ionophore stimulation. Nucleotide sequence analysis demonstrated no mutations in an open reading frame of c-kit cDNA and genomic DNA of the juxtamembrane domain of c-kit in HRMC cells. STI571 did not show any inhibitory effects on the proliferation of HRMC cells. These findings clearly demonstrated the existence of c-kit mutations-independent neoplastic canine mast cell proliferation. The growth factor-independent mast cell line established in this study might be valuable to explore novel mechanisms of c-kit mutations-independent neoplastic proliferation of mast cells in dogs. 相似文献
85.
Saki Ishimaru Mabrouk Elsabagh Seiichi Saiki Atsushi Haruno Maki Nakamura Hidetada Funo Taketo Obitsu Izuru Shinzato Toshihisa Sugino 《Animal Science Journal》2019,90(8):932-938
Three rumen‐protected lysine (RPL) products (AjiPro®‐L, LysiPEARLTM, and Feedtech Bypass LysineTM: A, B, and C, respectively) were tested for stability in two forage‐based total mixed rations (TMR1, 41.3% dry matter (DM), and TMR2, 49.5% DM) (experiment 1) and for Brix value (experiment 2). In experiment 1, each RPL product (2 g each) and TMR diet (200 g) were mixed and stored in plastic bags at 20°C for 0, 1, 3, 6, 12, 24, and 48 hr. In experiment 2, each RPL product (2 g) was dispensed into ion‐exchanged water (20 ml) and kept at 20°C for 0, 0.5, 1, 3, 6, 12, 24, and 48 hr. At each time point, free lysine (Lys) content and Brix values of extracts were measured, and Lys release (LR, %) was calculated. All RPL products LR% varied with varying diets DM and increased with increasing of time exposed to diets; it was highest in C, followed by B, and then A. Water LR% positively correlated with that from diets and with Brix values of Lys dissociated in water. Our results indicated that Lys dissociation from RPL products is affected by diet DM content. Brix value may be used as a potential marker for RPL protection efficacy. 相似文献
86.
Yuka Maeda Keiko Nishimura Kazuhiro Kurosu Hitoshi Mizuguchi Shigeru Sato Fuminori Terada Shiro Kushibiki 《Animal Science Journal》2019,90(4):523-532
This study aimed to examine the effects of feeding kraft pulp (KP) on the growth performance, feed digestibility, and rumen fermentation of Japanese Black fattening steers. Ten Japanese Black fattening steers (aged 26 months) were randomly divided into control and KP groups. The control group (n = 5) was fed concentrate feed without KP, and the KP group (n = 5) was fed concentrate feed containing 10% KP. Both the groups were provided rice straw as roughage. The experiment was conducted over a period of 12 weeks. There was no significant difference in dry matter intake, daily body weight gain, and nutrient digestibility between both groups. No difference was observed in the ruminal concentrations of volatile fatty acids among the groups. At weeks 8 and 12 after the onset of the experiment, the acetate‐to‐propionate ratio in the ruminal fluid of the KP group was significantly higher than that of the control group. The average daily pH of ruminal fluid and activity of ruminal lipopolysaccharide did not differ between the groups. Our results suggested that the growth performance and feed digestibility in the Japanese Black fattening steers were not influenced by replacing concentrate feed with KP. 相似文献
87.
Ogawa T Gamoh K Aoki H Kobayashi R Etoh M Senda M Hirayama N Nishimura M Shiraishi R Servat A Cliquet F 《Zoonoses and public health》2008,55(6):323-327
A virus neutralizing test using an indirect immunoperoxidase technique (VNT-IIP) for rabies has been developed for the titration of dog and cat serum samples in Japan. The VNT-IIP has the advantage that results obtained can be viewed by the naked eye. The purpose of this study was to validate the VNT-IIP and compare it with one of the international standard methods, the fluorescent antibody virus neutralization test (FAVNT). The VNT-IIP showed satisfactory repeatability, high analytical specificity and good accuracy. Regarding the comparison between the VNT-IIP and the FAVNT, the VNT-IIP showed good agreement (91.9%), high sensitivity (92.8%) as well as specificity (87.0%) and good correlation (r = 0.92). As described above, the validation of the VNT-IIP was satisfactory and the performances of the test proved to be equivalent to those of an international standard method. 相似文献
88.
Leandro G. Franco Carlos Henrique M. Wilges Daniel P. Junior Sofia A. Cerejo Lilian T. Nishimura Isabela P. Bittar 《Veterinary anaesthesia and analgesia》2018,45(3):250-259
Objective
To evaluate the effects of ketamine continuous rate infusions (CRI) at two dose rates on cardiovascular function and serum creatine kinase MB isoenzyme (CK-MB) and troponin I in healthy conscious dogs.Study design
Experimental, prospective, crossover, randomized, blinded study.Animals
Eight adult mixed-breed dogs, aged 6 ± 1 years and weighing 19 ± 8.6 kg (mean ± standard deviation).Methods
Dogs were administered an intravenous bolus of ketamine (0.5 mg kg?1) followed by a ketamine CRI for 12 hours (20 μg kg?1 minute?1; treatment TC20 or 40 μg kg?1 minute?1; treatment TC40). Sedation, heart rate (HR), mean arterial pressure (MAP), electrocardiographic and echocardiographic parameters were evaluated at baseline (T0) and 1 (T1), 2 (T2), 4 (T4), 8 (T8), 12 (T12) and 24 (T24) hours after ketamine infusion started. Serum concentrations of CK-MB and troponin I were measured at baseline and 12, 24 and 48 hours after infusion started.Results
HR increased over the first 4 hours, significantly at T1 in TC20 and at T4 in TC40 when compared with T0 (p < 0.05). MAP was significantly increased at T2 in TC40 when compared with TC20. Behavioral changes, such as stereotypical head movements and twitches, occurred within 4 hours in TC40. There were no significant changes in echocardiographic examinations in any dog when compared with baseline. There were no temporal changes in serum CK-MB activity either within or between treatments (p > 0.05). No troponin I was detected in any sample.Conclusions and clinical relevance
No indication of myocardial injury resulting from ketamine infusion was detected in this study in healthy dogs. Further studies are needed to assess the ketamine infusion effects on antinociception and other organ function not evaluated in the present study. 相似文献89.
Genetic male sterility is a useful trait in plant breeding, especially in angiosperm crops such as corn, onion and carrot.
We found a male sterile sugi (Cryptomeria japonica D. Don) tree in Toyama, Japan. Pollen of sugi is one of the major causes of pollinosis in Japan. We carried out this research
in an attempt to make clear the characteristics and inheritance of this male sterility. Microsporogenesis of the male sterile
tree proceeded meiosis, however, the microspores collapsed after they were separated from pollen tetrads in locules, resulting
in complete male sterility. Most likely, ethylene evolution was responsible for male sterility expression. Full seed setting
in the male sterile tree indicated normal macrosporogenesis. Seeds obtained from crossing between male sterile and normal
lines showed relatively high level of germination and their seedlings grew vigorously. The somatic chromosome numbers of 241
germinated seeds, derived from the male sterile tree, were mostly 22, euploid. These results indicated that male sterile tree
was different from other similar previously reported trees with low pollen fertility, resulting from triploid or trisomics.
Probably, male sterility in sugi is either nuclear genetic male sterility or cytoplasmic male sterility.
The study was partially supported by Program for Promotion of Basic Research Actives for Innovative Biosciences. 相似文献
90.
Hisashi Nishiwaki Yoshiaki Nakagawa David Y Takeda Atsushi Okazawa Miki Akamatsu Hisashi Miyagawa Tamio Ueno Keiichiro Nishimura 《Pest management science》2000,56(10):875-881
Variously substituted benzyl derivatives of chloronicotinyl insecticides were synthesized with a wide range of substituents including halogens, NO2, CN, CF3 and small alkyl and alkoxy groups at the ortho, meta and para positions, as well as multiple‐substituted benzyl analogues. Their binding activity to the α‐bungarotoxin binding site in housefly (Musca domestica) head membrane preparations was measured. Among the compounds tested, the activity of the meta‐CN derivative was the highest, being 20–100 times higher than those of imidacloprid, acetamiprid and nitenpyram. The synergized insecticidal activity against houseflies was also measured for selected compounds with the metabolic inhibitor, NIA16388 (propargyl propyl phenylphosphonate). For the nitromethylene analogues, including both benzyl and pyridylmethyl analogues, higher binding activity usually resulted in higher insecticidal activity. © 2000 Society of Chemical Industry 相似文献