Evaluation of intra-articular injection of autologous platelet lysate (PL) in horses with osteoarthritis of the distal interphalangeal joint

Background: Regenerative medicine has become one of the most promising therapies of equine osteoarthritis. Platelet lysate (PL) is rich in bioactive proteins and growth factors that play a crucial role in tissue healing.

Objective: To evaluate the efficacy of intra-articularly injected autologous PL in equine athletes with naturally occurring osteoarthritis.

Animals and methods: Fifteen warmblood geldings aged 8–19 years with osteoarthritis of the distal interphalangeal joint were included in this study. They were randomly divided into two groups; 10 horses received intra-articular injections of PL and 5 of normal saline (controls). Before treatment, platelet-derived growth factor (PDGF) levels in basal plasma and prepared PL were estimated. Each joint was injected twice within a three-week period. Lameness was evaluated using the American Association of Equine Practitioners grading system, before treatment and 10 days after each intra-articular injection. Horses were examined fortnightly for one year. Radiographic examination was performed six months post-treatment. The generalized estimating equation test was used for statistical analysis.

Results: Acceptable levels of PDGF were detected in PLs (mean ± SD: 258.0 ± 52.3 pg/ml). The majority of horses (9/10) responded positively to PL treatment presenting lower lameness grades (p < 0.0005) compared to controls 10 days after the second injection, and returned to normal athletic activity. Radiographs revealed no changes in osteoarthritis lesions six months after treatment. One year post-injections, however, all horses relapsed to their initial degree of lameness.

Conclusion: Intra-articularly injected autologous PL is an efficient method for temporarily managing osteoarthritis of the distal interphalangeal joint in athletic horses.     

Evaluation of ornithine carbamoyl transferase and other serum and liver-derived analytes in diagnosis of fatty liver and postsurgical outcome of left-displaced abomasum in dairy cows

OBJECTIVE: To evaluate postsurgical outcome in dairy cows with left-displaced abomasum (LDA) with regard to severity of fatty liver and assess the usefulness of preoperative determination of serum ornithine carbamoyl transferase (OCT) activity, bile acids concentration, and other variables for evaluating liver function during the postsurgical convalescence period. DESIGN: Prospective study. ANIMALS: 68 Holstein cows. PROCEDURES: Blood and liver biopsy specimens were obtained during standing LDA surgery. Liver tissue was examined histologically and classified by severity of fatty change. Serum activities of liver-derived enzymes and concentrations of total lipids, triglycerides, bile acids, glucose, beta-hydroxybutyric acid, bilirubin, and nonesterified fatty acids were determined. RESULTS: Most cows with LDA and cows with severe fatty liver were detected within the first month after calving. Postsurgical outcome was related to severity of fatty liver. All cows that died had severe fatty liver. Serum activities of OCT, aspartate aminotransferase, and glutamate dehydrogenase and serum total bilirubin concentration were sensitive indicators of fatty liver. Serum bile acids concentration was not an accurate indicator of fatty liver. CONCLUSIONS AND CLINICAL RELEVANCE: Postsurgical outcome of cows undergoing surgery to correct LDA was related to fatty liver severity. Assessment of serum activities of OCT, aspartate aminotransferase, and glutamate dehydrogenase and serum total bilirubin concentration is recommended for diagnosis of fatty liver in dairy cows with LDA, whereas determination of bile acids concentration is not. The strong correlation between OCT activity and degree of hepatocellular damage supports use of this enzyme for assessing severity of fatty liver and predicting postsurgical outcome in cows with LDA.     

Simultaneous determination of terbuthylazine and its major hydroxy and dealkylated metabolites in wetland water samples using solid-phase extraction and high-performance liquid chromatography with diode-array detection

A method based on high-performance liquid chromatography with diode-array detection was developed and validated aiming at the simultaneous determination of terbuthylazine (TER) and its five major metabolites, desisopropyl-hydroxy-atrazine, desethyl-hydroxy-terbuthylazine, desisopropyl-atrazine, hydroxy-terbuthylazine, and desethyl-terbuthylazine. Although s-triazines are used worldwide as herbicides for agricultural and nonagricultural purposes, there is limited information on the environmental impact of TER degradation products. The proposed method includes a solid-phase extraction procedure (using MCX cartridges) with adequate recovery efficiency (70-80%). The statistical evaluation of the method reveals good linearity, accuracy, and precision for the compounds determined, with RSD values less than 14.6%, while the detection limit was found to be 0.05 microg L(-1) for DIHA and 0.01 microg L(-1) for the other substances. This method can be employed in biodegradation studies of TER and its metabolites in water samples from constructed wetlands, thus assisting the evaluation of their environmental impact.     

Effect of variety choice, resistant rootstocks and chitin soil amendments on soil-borne diseases in soil-based, protected tomato production systems

Soil-borne diseases are the most significant crop protection problem in soil-based, low-input and especially organic glasshouse production systems in Europe. While chemical soil disinfestation has been the control method of choice in conventional farming systems, soil steaming has been the main strategy for the control of soil-borne diseases in organic production. Both methods are extremely expensive and have been increasingly restricted for environmental reasons by governments, and integrated and organic farming standard-setting bodies. The use of disease-tolerant varieties, grafting onto resistant rootstocks and chitin soil amendments were evaluated as potential replacements for soil steaming in organic and other low-input tomato production systems. When only Pyrenochaeta lycopersici and/or Meloidogyne spp. were present in soil, grafting and/or chitin soil amendment were found to be as effective in reducing root disease and/or increasing yield as soil steaming, but the efficacy of both treatments was reduced when Verticillum albo-atrum was also present in soil. No additive effects of combining grafting and chitin soil amendments could be detected. A more widespread use of grafting and/or chitin soil amendments may therefore allow significant reductions in the use of steam and chemical soil disinfestation in glasshouse crops. It will also allow integrated and organic farming standard-setting bodies to impose further restrictions on the use of soil disinfestation treatments.     

Diversity among melon landraces (Cucumis melo L.) from Greece and their genetic relationships with other melon germplasm of diverse origins

Diversity among 17 melon landraces and inbred lines of Group Cantalupensis, Inodorus, and Flexuosus germplasm from Greece was assessed using 24 RAPD primers, 11 morphological traits of fruit, two yield-related characteristics, and resistance to powdery mildew. Accessions were genetically diverse and the greatest variation was detected in Group Flexuosus. Comparative analysis of Greek germplasm and an array of previously characterized reference accessions [RA; 68 from Africa (15), United States (U.S.; 5), Central Europe (14), Japan (19), and Spain (15)], spanning major market classes employing 19 RAPD primers (standard marker array), indicates that all but one of the accessions from Greece showed genetic affinities among themselves and with 23 RA of various origin (i.e., Japan, U.S., Central Europe, Spain and Israel) and market class. Accessions from Greece were, however, distinct from the African landraces and most Spanish melons. Greek accessions also showed little genetic affinity with the Casabas (Blanco and Crenshaw), Ogen, and U.S. Eastern Market accessions. Group Flexuosus and Inodorus accessions from Greece possessed genetic affinities with Western Asian (Group Conomon) and Mediterranean (Group Inodorus) market classes, respectively. The uniqueness of Greek melon landraces portends their potential usefulness for the enhancement of U.S., European, and Asian melon market classes. This revised version was published online in July 2006 with corrections to the Cover Date.     

Effect of dietary dried tomato pulp on oxidative stability of Japanese quail meat

Ninety, 21-day-old, Japanese quail (Coturnix coturnix japonica) divided into three groups with five subgroups each were fed a basal diet that served as control or a basal diet containing 5 or 10% of dried tomato pulp (DTP), a byproduct of the tomato-processing industry. The DTP contained lycopene and beta-carotene at 281 and 24.3 mg kg(-)(1) of dry weight, respectively. On day 42 of age, birds were slaughtered, and carcasses were trimmed for breast meat. To assess the effect of dietary treatment on the oxidative stability of raw and cooked meat, raw meat was subjected to iron-induced lipid oxidation, whereas both raw and cooked meats were subjected to refrigerated storage at 4 degrees C. The extent of lipid oxidation was determined on the basis of the malondialdehyde (MDA) formed through the use of third-order derivative spectrophotometry. Results showed that after 6 and 9 days of refrigerated storage, MDA values in raw meat were increased. The increase was higher (P < 0.05) for the 10% DTP group and lower (P < 0.05) for the 5% DTP group, compared to control. An analogous oxidation profile was observed for cooked meat at 3, 6, and 9 days of storage. Iron-induced lipid oxidation of raw meat showed that the 10% DTP group as well as the control group exhibited MDA values that did not differ (P > 0.05) from each other at all time points, whereas the 5% DTP group presented MDA values that, although not differing from those of the other groups at 0 and 50 min, were significantly (P < 0.05) lower than those of the other groups at 100 and 150 min of iron-induced lipid oxidation. These results suggested that inclusion of dried tomato pulp in feed at a level of 5% exerted an antioxidant effect, whereas addition at level of 10% exerted a prooxidant effect. Mean alpha-tocopherol levels in the control, 5% DTP, and 10% DTP groups were 2.2, 2.1, and 1.4 mg kg(-)(1) of meat, respectively. Fatty acid analysis showed that the 10% DTP group had a higher (P < 0.05) content of total polyunsaturated fatty acids and a greater (P < 0.05) unsaturated/saturated fatty acid ratio compared to control. There might be an interaction between DTP and alpha-tocopherol that is of importance for the balance between pro- and antioxidative activities. Future experiments should be designed to explore the interaction between individual carotenoids and tocopherols in order to better elucidate their role in oxidative changes.     

Modeling of arsenic immobilization by zero valent iron

Ground waters in geothermal regions contain arsenic concentrations that exceed the recommended drinking water standards. In addition, when these regions have agricultural activities, the waters also contain high levels of nitrates and phosphates. These contaminants can be removed from the water with the use of filters containing zero valent iron (ZVI). The objective of this study was to model the removal of arsenate (As(V)) and arsenite (As(III)) by ZVI and to model the effect of competing ions (phosphate and nitrate) on arsenate removal. Arsenic immobilization by ZVI columns was simulated by the HM-1D chemical transport and speciation model and an one-dimensional analytical solution model. Laboratory column studies were conducted in order to obtain representative experimental data for simulation with both models. Arsenic speciation and the presence of competing ions greatly affect arsenic removal by ZVI. Most arsenic is precipitated/co-precipitated on ZVI and on the corrosion products formed on ZVI. The simulations suggested that As(V) process parameters are higher than the As(III) parameters and that they are affected by the presence of nitrates and phosphates in the system. Such models can be used to design treatment units by incorporating the impact of nitrates and phosphates in the removal of arsenic by ZVI as well as the impact of temperature on the process.     

In vitro investigation into the potential prebiotic activity of honey oligosaccharides

The effect of honey oligosaccharides on the growth of fecal bacteria was studied using an in vitro fermentation system. Prior to treatment, glucose and fructose (31.73 and 21.41 g/100 g of product, respectively) present in honey, which would be digested in the upper gut, were removed to avoid any influence on bacterial populations in the fermentations. Nanofiltration, yeast (Saccharomyces cerevisiae) treatment, and adsorption onto activated charcoal were used to remove monosaccharides. Prebiotic (microbial fermentation) activities of the three honey oligosaccharide fractions and the honey sample were studied and compared with fructooligosaccharide (FOS), using 1% (w/v) fecal bacteria in an in vitro fermentation system (10 mg of carbohydrate, 1.0 mL of basal medium). A prebiotic index (PI) was calculated for each carbohydrate source. Honey oligosaccharides seem to present potential prebiotic activity (PI values between 3.38 and 4.24), increasing the populations of bifidobacteria and lactobacilli, although not to the levels of FOS (PI of 6.89).