全文获取类型
收费全文 | 640篇 |
免费 | 53篇 |
专业分类
林业 | 47篇 |
农学 | 7篇 |
基础科学 | 2篇 |
80篇 | |
综合类 | 61篇 |
农作物 | 20篇 |
水产渔业 | 33篇 |
畜牧兽医 | 383篇 |
园艺 | 4篇 |
植物保护 | 56篇 |
出版年
2024年 | 1篇 |
2023年 | 9篇 |
2022年 | 11篇 |
2021年 | 27篇 |
2020年 | 15篇 |
2019年 | 22篇 |
2018年 | 15篇 |
2017年 | 20篇 |
2016年 | 23篇 |
2015年 | 18篇 |
2014年 | 32篇 |
2013年 | 24篇 |
2012年 | 63篇 |
2011年 | 54篇 |
2010年 | 28篇 |
2009年 | 36篇 |
2008年 | 44篇 |
2007年 | 51篇 |
2006年 | 47篇 |
2005年 | 36篇 |
2004年 | 28篇 |
2003年 | 16篇 |
2002年 | 25篇 |
2001年 | 5篇 |
2000年 | 3篇 |
1999年 | 4篇 |
1998年 | 6篇 |
1997年 | 4篇 |
1996年 | 2篇 |
1995年 | 2篇 |
1994年 | 2篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1991年 | 2篇 |
1988年 | 1篇 |
1987年 | 3篇 |
1986年 | 3篇 |
1985年 | 1篇 |
1983年 | 1篇 |
1981年 | 1篇 |
1979年 | 1篇 |
1974年 | 1篇 |
1972年 | 1篇 |
1969年 | 1篇 |
1967年 | 1篇 |
1958年 | 1篇 |
排序方式: 共有693条查询结果,搜索用时 15 毫秒
101.
The herbicide glufosinate‐ammonium (GLA) is a competitive inhibitor of glutamine synthetase (GS), an enzyme converting glutamate to glutamine in both plants and animals. Because GS is essential for ammonia detoxification in plants, GLA treatment disrupts photorespiration by causing a build‐up of ammonia and a loss of glutamine in plant tissues. This study reports that GLA applied to leaf surfaces is also toxic to 5th‐instar caterpillars of the skipper butterfly Calpodes ethlius (LD50 = 400 mg kg−1). After ingesting GLA, caterpillars stopped feeding and became dehydrated through a loss of rectal function. Caterpillars showed symptoms of neurotoxicity, such as proleg tremors, body convulsions and complete paralysis before death. Incubation of several tissues isolated from normal feeding‐stage caterpillars with the GS substrates glutamate and ammonium showed that GLA inhibited GS activity in vitro. Within 24 h of ingesting GLA, caterpillars had a greatly reduced glutamine content and the ammonium ion levels had more than doubled. Injection of ammonium chloride into non‐GLA‐treated caterpillars had no deleterious effect, suggesting that glutamine depletion, and not a rise in body ammonium, was the primary cause of GLA toxicity following GS inhibition. This was supported by the observation that the onset of the symptoms of GLA poisoning could be postponed by giving GLA‐fed caterpillars several subsequent daily injections of glutamine. The effective GLA dose fed to 5th‐instar caterpillars in this study was comparable to the amount that might realistically be acquired from feeding on GLA‐treated crops. © 2001 Society of Chemical Industry 相似文献
102.
The extractability and molecular weight of β-glucan in oat bran, oat bran muffins, and oat porridge and the changes taking place during processing and storage were studied. The β-glucan was extracted using hot water and a thermostable α-amylase and by an in vitro system that simulated human digestion. Molecular weight (MW) of the extracted β-glucan was determined using high-performance size-exclusion chromatography. Hot-water treatment extracted 50–70% of total β-glucan in oat bran samples and rolled oats. The chromatographic peak MW of extracted β-glucan was in the 1.4–1.8 × 106 range. Using the in vitro digestion system, 12–33% of total β-glucan in bran and rolled oats was solubilized, and peak MW was in the same range as β-glucan extracted by hot-water treatment. In muffins, 30–85% of total β-glucan was solubilized by in vitro digestion, with a major difference in extractability among muffins from different recipes. Peak MW of extracted β-glucan was lower in all muffins when compared to original bran. During frozen storage, extractable β-glucan decreased by >50% in all muffins, but no change in peak MW of extracted β-glucan was detected. 相似文献
103.
104.
Genevieve Patrick Andrea M. Tarnecki Nicole Rhody Ryan Schloesser Kevan Main Roy Yanong Ruth Francis‐Floyd 《Aquaculture Research》2019,50(12):3793-3801
Almaco jack (Seriola rivoliana Valenciennes) is an excellent candidate for aquaculture due to its fast growth rate and high market value. While S. rivoliana have adapted well to captivity, survival at early life stages can be improved to increase profitability during production. A wide range of variables cause larval mortalities but high bacterial loads in rearing tanks are often correlated with these losses. The aim of this study was to investigate the effect of egg disinfection on bacterial load and hatch rate of S. rivoliana. Disinfectants tested included formalin (F100 and F200; 100 and 200 mg/L, respectively, for 60 min), hydrogen peroxide (HPO; 300 mg/L for 10 min) and peracetic acid/hydrogen peroxide (PAA/HPO; 15.7 mg/L/39.6 mg/L for 1 min). Concentrations and contact times were determined based on current use in marine aquaculture and preliminary trials. Eggs treated with HPO and F100 had significantly higher hatch rates than the untreated control group. All treatments significantly decreased total Vibrio counts compared to untreated eggs; however, total bacterial counts were only decreased following treatments with PAA/HPO and F200. To prevent egg mortality due to bacterial overgrowth, consideration should be given to the use of surface disinfection using HPO or F100. Future studies should investigate the use of peracetic‐based products at lower doses. 相似文献
105.
Andrea Arias Aguirre Bruno Studer Javier Do Canto Ursula Frei Thomas Lübberstedt 《Plant Breeding》2014,133(6):765-770
Perennial ryegrass (Lolium perenne L.) displays a two‐locus gametophytic self‐incompatibility (SI) system that remains intact at the tetraploid level. Two models are plausible for SI in autotetraploids. In Model I: both alleles at the S locus and both at the Z locus in diploid pollen matching the female genotype results in incompatibility. In Model II: only one allele at S and one at Z locus in diploid pollen matching the female results in incompatibility. The goals were to determine which of the models best explains SI in our autotetraploid ryegrass population and to evaluate the efficiency of high‐resolution melting (HRM) genotyping for discriminating different iso‐allelic genotypes. The progeny of a cross between two autotetraploids was characterized with three HRM‐based markers co‐segregating with Z. Segregation ratios were used to make inferences about the mode of action of the SI system. The observed segregation differed significantly (P < 0.001) from the expected under Model I, but not from the expected under Model II (P = 0.463). Thus, Model II explains SI in this population, and HRM is an efficient tool to distinguish different iso‐allelic genotypic classes. 相似文献
106.
107.
Carl-Christian Gelfert Antje Lptien Nicole Montag Rudolf Staufenbiel 《Research in veterinary science》2009,86(3):529-532
Seeing the fact that farm managers in Germany feed anionic salts to transition cows once daily, this study set out to evaluate whether the effects on the acid–base status (ABS) and calcium excretion in urine would persist throughout the entire day beyond this feeding practice. Eleven non-lactating, non-pregnant, Holstein–Friesian-cows with a rumen fistula were administered 2Eq of calcium chloride (CaCl2/five cows) or calcium sulfate (CaSO4/six cows) once daily for a period of 1 week. At day 7, blood and urine samples were taken every 4 h starting at 06:00 a.m. before feeding the anionic salts, and then ending at the same time the next day. Feeding anionic salts to the cows induced metabolic acidosis in both of the groups. The changes tended to be greater in CaCl2-cows. After 12 h, the acidosis lessened and the initial values were reached after 24 h. The CaCl2-cows, however, still showed signs of compensated metabolic acidosis. The results of the present study showed that feeding anionic salts once daily confined the risk of an interrupted effect of the anionic salts on the acid–base status as well as calcium metabolism after 12 h. 相似文献
108.
109.
Sachse K Vretou E Livingstone M Borel N Pospischil A Longbottom D 《Veterinary microbiology》2009,135(1-2):2-21
There are two main approaches to diagnosing infections by Chlamydia and Chlamydophila spp. in mammals and birds. The first involves the direct detection of the agent in tissue or swab samples, while the second involves the serological screening of blood samples for the presence of anti-chlamydial antibodies. Ultimately, the test that is used is dependent on the types of samples that are submitted to the diagnostic laboratory for analysis. The present paper gives an overview on methodologies and technologies used currently in diagnosis of chlamydial infections with emphasis on recently developed tests. The performance characteristics of individual methods, such as the detection of antigen in smears and in pathological samples, the isolation of the pathogen, various antibody detection tests and DNA-based methods utilising conventional and real-time PCR, as well as DNA microarray technology are assessed, and specific advantages and drawbacks are discussed. Further, a combination of a specific real-time PCR assay and a microarray test for chlamydiae is proposed as an alternative reference standard to isolation by cell culture. 相似文献
110.
Nicole I. Stacy Mary B. Nabity Nicole Hackendahl Melanie Buote Jennifer Ward Pamela E. Ginn William Vernau William L. Clapp John W. Harvey 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2009,38(1):113-120
Abstract: Two young adult dogs with gastrointestinal signs were each found to have an intra‐abdominal mass based on physical examination and diagnostic imaging. On exploratory laparotomy, small intestinal masses and mesenteric lymphadenopathy were found in both dogs; a liver mass was also found in dog 1. Cytologic and histologic examination of intestinal and liver masses and mesenteric lymph nodes revealed 2 distinct lymphoid cell populations: lymphoblasts and atypical Mott cells. With Romanowsky stains, the atypical Mott cells contained many discrete, clear to pale blue cytoplasmic inclusions consistent with Russell bodies that were positive by immunohistochemistry for IgM and CD79a in both dogs and for IgG in dog 2. The Mott cells and occasional lymphoblasts stained strongly positive with periodic acid‐Schiff. Using flow cytometric immunophenotyping in dog 1, 60% of peripheral blood mononuclear cells and 85% of cells in an affected lymph node were positive for CD21, CD79a, IgM, and MCH II, indicative of B‐cells. With electron microscopy, disorganized and dilated endoplasmic reticulum was seen in Mott cells in tumors from both dogs. Antigen receptor gene rearrangement analysis of lymph node and intestinal masses indicated a clonal B‐cell population. Based on cell morphology, tissue involvement, and evidence for clonal B‐cell proliferation, we diagnosed neoplasms involving Mott cells. To the authors' knowledge, this is the second report of Mott cell tumors or, more appropriately, B‐cell lymphoma with Mott cell differentiation, in dogs. More complete characterization of this neoplasm requires further investigation of additional cases. This lymphoproliferative disease should be considered as a differential diagnosis for canine gastrointestinal tumors. 相似文献