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591.
Neumann EJ Grinberg A Bonistalli KN Mack HJ Lehrbach PR Gibson N 《Veterinary microbiology》2009,138(3-4):297-303
The porcine reproductive and respiratory syndrome virus (PRRSV) is an enveloped RNA virus. Virions of PRRSV contain six membrane proteins: the major proteins GP5 and M and the minor proteins GP2, GP3, GP4, and E. The GP5 is the major envelope proteins, which was involved in the formation and infectivity of PRRSV by coaction with other membrane proteins. Here, to determine the function of alone GP5 envelope protein in viral entry, we investigated the formation and infectivity of GP5-pseudotyped virus particles. By co-transfection of GP5 expression plasmids with murine leukemia virus (MuLV) based retroviral vectors (pHIT60, encoding MuLV Gag-Pol; pHIT111, encoding an MuLV genome with a β-galactosidase reporter gene) into 293 T cells and analysis of the culture medium using ultracentrifugation, Western blot, and infection assay. We observed that the GP5 envelope protein was incorporated into the MuLV retroviral vectors to generate an pseudotyped murine leukemia virus, which was infectious to PAM and Mack-145 target cells and displayed the same host range with wild-type PRRSV. The infection of the pseudotyped virus on PAM target cells is effectively neutralized by polyclonal antibodies specific for PRRSV or GP5. The results suggested that the GP5 protein may play a key role in the viral entry by interacting with the host cell receptor. The GP5-pseudotyped virus will be useful in the identification of the cellular receptor binding with GP5 protein. 相似文献
592.
Ruhl S Casson N Kaiser C Thoma R Pospischil A Greub G Borel N 《Veterinary microbiology》2009,135(1-2):169-174
Bovine abortion of unknown infectious aetiology still remains a major economic problem. In this study, we focused on a new possible abortigenic agent called Parachlamydia acanthamoebae. Retrospective samples (n=235) taken from late-term abortions in cattle were investigated by real-time diagnostic PCR for Chlamydiaceae and Parachlamydia spp., respectively. Histological sections of cases positive by real-time PCR for any Chlamydia-related agent were further examined by immunohistochemistry using specific antibodies. Chlamydophila abortus was detected only in three cases (1.3%) by real-time PCR and ArrayTube Microarray playing a less important role in bovine abortion compared to the situation in small ruminants in Switzerland. By real-time PCR as many as 43 of 235 (18.3%) cases turned out to be positive for Parachlamydia. The presence of Parachlamydia within placental lesions was confirmed in 35 cases (81.4%) by immunohistochemistry. The main histopathological feature in parachlamydial abortion was purulent to necrotizing placentitis (25/43). Parachlamydia should be considered as a new abortigenic agent in Swiss cattle. Since Parachlamydia may be involved in lower respiratory tract infections in humans, bovine abortion material should be handled with care given the possible zoonotic risk. 相似文献
593.
Ariane Schweighauser Dr med vet DACVIM ; Iwan A. Burgener Dr med vet DACVIM DECVIM ; Frédéric Gaschen Dr med vet DACVIM DECVIM ; Nicole Luckschander Dr med vet DACVIM DECVIM ; Andreas Hasler Dr med vet DACVIM ; Johann Lang Dr med vet DECVDI Thierry Francey Dr med vet DACVIM 《Journal of Veterinary Emergency and Critical Care》2009,19(4):363-368
Objectives – This case series describes 5 dogs with small intestinal intussusception and acute kidney injury due to infection with Leptospira interrogans serovar Australis.
Case Series Summary – Small intestinal intussusception was observed in 4 dogs diagnosed with acute kidney injury due to leptospirosis presented between 1997 and 2005. Intussusception was diagnosed at initial presentation or later during hospitalization. An additional dog fulfilling our inclusion criteria was presented to a small animal specialty clinic nearby and was included. Upon admission, all dogs were severely azotemic and thrombocytopenic. All 5 dogs showed the strongest microscopic agglutination test serology reaction to L. interrogans serovar Australis. Two dogs survived with no apparent residual renal damage, 1 survived with subsequent mild chronic kidney disease, and 2 dogs were euthanized at the owners' request due to a guarded prognosis.
New or Unique Information Provided – Intussusception can occur or may be seen in dogs with leptospirosis due to L. interrogans serovar Australis and patients should be monitored closely for this potential complication. As all 5 dogs described in this case series showed the highest titer for L. interrogans serovar Australis, these precautions may be especially applied in geographic areas where this particular serovar is seen. 相似文献
Case Series Summary – Small intestinal intussusception was observed in 4 dogs diagnosed with acute kidney injury due to leptospirosis presented between 1997 and 2005. Intussusception was diagnosed at initial presentation or later during hospitalization. An additional dog fulfilling our inclusion criteria was presented to a small animal specialty clinic nearby and was included. Upon admission, all dogs were severely azotemic and thrombocytopenic. All 5 dogs showed the strongest microscopic agglutination test serology reaction to L. interrogans serovar Australis. Two dogs survived with no apparent residual renal damage, 1 survived with subsequent mild chronic kidney disease, and 2 dogs were euthanized at the owners' request due to a guarded prognosis.
New or Unique Information Provided – Intussusception can occur or may be seen in dogs with leptospirosis due to L. interrogans serovar Australis and patients should be monitored closely for this potential complication. As all 5 dogs described in this case series showed the highest titer for L. interrogans serovar Australis, these precautions may be especially applied in geographic areas where this particular serovar is seen. 相似文献
594.
Bruna Sayuri Fujiyama Mário Lopes da Silva Júnior Nicole Raquel Pinto Cardoso Adrianne Braga da Fonseca Rafael Gomes Viana 《Journal of plant nutrition》2013,36(19):2498-2506
AbstractInfluence of boron (B) on photosynthesis and water use has not sufficiently been evaluated in soybean despite its worldwide importance as a crop. The objective of this work was to evaluate the effects of B application on photosynthesis and water use in soybean at vegetative growth stage. A pot experiment was carried out in a greenhouse. Soybean [Glycine max (L.) Merrill cv. M8644 IPRO] was grown in a clayey Oxisol previously fertilized with 4 B rates (0, 1.5, 3.0, and 6.0?mg dm?3). Net photosynthesis rate (Pn), intercellular CO2 concentration (Ci), transpiration rate (E), stomatal conductance (gs) were measured in the second trifoliate leaf of plants at the V4 growth stage. Instantaneous water use efficiency (WUEis = Pn/E) and intrinsic water use efficiency (WUEic = Pn/gs) were calculated. B application to soil increased all these variables. The most consistent increases were in Pn, WUEis, and WUEic. The critical level of hot water extractable B in soil that maximized photosynthesis and optimized water use by soybean at vegetative growth stage was calculated to be 0.38?mg dm?3. 相似文献
595.
Fouchier RA García-Sastre A Kawaoka Y Barclay WS Bouvier NM Brown IH Capua I Chen H Compans RW Couch RB Cox NJ Doherty PC Donis RO Feldmann H Guan Y Katz J Klenk HD Kobinger G Liu J Liu X Lowen A Mettenleiter TC Osterhaus AD Palese P Peiris JS Perez DR Richt JA Schultz-Cherry S Steel J Subbarao K Swayne DE Takimoto T Tashiro M Taubenberger JK Thomas PG Tripp RA Tumpey TM Webby RJ Webster RG 《Science (New York, N.Y.)》2012,335(6067):400-401
596.
The purpose of this study was to describe the appropriate surgical technique, postoperative monitoring, and complications encountered with use of vacuum-assisted closure (VAC) in six dogs with confirmed septic peritonitis. Initial diagnosis of septic peritonitis was performed by measuring either the blood-to-fluid lactate ratio and glucose concentration differences or cytologic verification of intracellular bacteria. After appropriate surgical procedures were performed to manage the primary cause of peritoneal sepsis, a VAC was performed. Serum and abdominal fluid protein levels were measured, and all complications were noted during the postoperative period. Three of the six dogs (50%) survived to the secondary closure and were subsequent discharged, which is similar to previous studies where the abdomen was either closed primarily or treated with open abdominal drainage. No major complications occurred with bandage management during hospitalization. The results of this study support VAC as a feasible technique for managing septic peritonitis. 相似文献
597.
Liliana M. Marín M. Cristina Iazbik Sara Zaldivar‐Lopez Linda K. Lord Nicole Stingle Paulo Vilar Ana Lara‐Garcia Francisco Alvarez Kenji Hosoya Laura Nelson Antonio Pozzi Edward Cooper Mary A. McLoughlin Rebecca Ball William C. Kisseberth Cheryl A. London Robert Dudley Jonathan Dyce Melanie McMahon Phillip Lerche Richard Bednarski C. Guillermo Couto 《Journal of Veterinary Emergency and Critical Care》2012,22(3):332-340
598.
599.
Jeffrey H. Gertsch Bryan Corbett Peter S. Holck Allison Mulcahy Melanie Watts Nathan Thomas Stillwagon Amanda Morgan Casto Charles Hessel Abramson Charles Peter Aloysius Vaughan Christopher Macguire Neda Nicole Farzan Baotran Nguyen Vo Rebecca Jean Norvelle Kerstin May Jessica Elizabeth Holly Hillary Irons Aaron Michael Stutz Pradip Chapagain Siddhartha Yadav Matiram Pun Buddha Basnyat 《The Forestry Chronicle》2012,23(4):307-315
600.
Büttner N Beineke A de Buhr N Lilienthal S Merkel J Waldmann KH Valentin-Weigand P Baums CG 《Veterinary immunology and immunopathology》2012,146(3-4):191-200
Streptococcus suis diseases in pigs, most importantly meningitis, are worldwide responsible for major economic losses in the pig industry. About one fourth of invasive S. suis diseases are caused by S. suis serotype 9 strains in Europe. However, little is known about serotype 9 since most studies were performed with serotype 2. The objective of this study was to determine the immunogenicity and protective efficacy of a serotype 9 bacterin in piglets. Challenge was conducted with a reference serotype 9 strain, belonging to the same clonal complex but to a different sequence type as the bacterin strain. The bacterin induced protection against mortality but not morbidity. Eleven days post infection, 3 of 7 vaccinated survivors were not fully convalescent and had not eliminated the challenge strain from inner organs completely. In accordance with the clinical findings, the majority of piglets showed fibrinous-suppurative lesions in at least one inner organ or tissue. In contrast to the placebo group such lesions were not detected in one third of bacterin-vaccinated piglets. Determination of specific serum IgG titers revealed that the bacterin elicited seroconversion against muramidase-released protein and basic membrane lipoprotein. Furthermore, vaccination was associated with induction of opsonizing antibodies against the serotype 9 challenge strain. However, titers of opsonizing antibodies were rather low in comparison to those found in our previous serotype 2 vaccination trial. Piglets developed substantially higher titers of opsonizing antibodies after challenge. Opsonizing antibodies were absorbable with the serotype 9 challenge strain but not with an unencapsulated isogenic mutant of a serotype 2 strain indicating their specificity. The results indicate that a serotype 9 bacterin is less protective than a serotype 2 bacterin, most likely due to inducing only low titers of opsonizing antibodies. This might contribute to emergence of serotype 9 strains, in particular strains of this clonal complex, in Europe. 相似文献