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71.
Andraud M Rose N Laurentie M Sanders P Le Roux A Cariolet R Chauvin C Jouy E 《Veterinary research》2011,42(1):44
Antimicrobial resistance is of primary importance regarding public and animal health issues. Persistence and spread of resistant strains within a population contribute to the maintenance of a reservoir and lead to treatment failure. An experimental trial was carried out to study the horizontal transmission of a fluoroquinolone-resistant Escherichia coli strain from inoculated to naïve pigs. All naïve contact pigs had positive counts of fluoroquinolone-resistant E. coli after only two days of contact. Moreover, re-infections of inoculated pigs caused by newly contaminated animals were suspected. A maximum likelihood method, based on a susceptible-infectious-susceptible (SIS) model, was used to determine the transmission parameters. Two transmission levels were identified depending on the quantity of bacteria shed by infected individuals: (i) low-shedders with bacterial counts of resistant E. coli in the faeces between 5*103 and 106 CFU/g (βL = 0.41 [0.27; 0.62]), (ii) high shedders with bacterial counts above 106 CFU/g (βH = 0.98 [0.59; 1.62]). Hence, transmission between animals could be pivotal in explaining the persistence of resistant bacteria within pig herds. 相似文献
72.
Ammonia in cigarette mainstream smoke was quantified by LC-MS/MS after derivatization. Two different reagents, fluorescamine and dansyl chloride, were investigated, but only the latter gave stable derivatives; therefore, it was considered the most appropriate choice. Smoke samples were collected on a Cambridge filter pad followed by an impinger containing a solution of hydrochloric acid. Ammonia was then derivatized with a 18.5 mM solution of dansyl chloride in acetonitrile at 70 °C for 30 min in a vial with the internal standard, (15)ND(4)Cl. The resulting derivative was analyzed by LC-MS/MS detection with an atmospheric pressure chemical ionization (APCI) interface in the positive ionization mode using multiple-reaction monitoring (MRM). Good linearity was obtained in the concentration range of 0.02-1.65 μg/mL (r(2) ≥ 0.999), and the limit of detection (LOD) was established at 0.006 μg/mL. This method has the advantage of being sensitive, efficient, and reliable and is not hindered by interferences from the sample matrix. It should thus be considered a reference method of choice for the determination of ammonia in smoke. 相似文献
73.
74.
Wefel S Mendez-Angulo JL Ernst NS 《The Canadian veterinary journal. La revue veterinaire canadienne》2011,52(8):884-887
A 4-year-old Quarter horse stallion was presented for acute abdominal pain. Exploratory celiotomy revealed a mesenteric diverticulum of the jejunum and mesodiverticular band that were associated with small intestinal strangulation. Resection and anastomosis were performed. A second celiotomy was performed due to adhesions. The horse recovered completely and returned to training. 相似文献
75.
Crépin S Chekabab SM Le Bihan G Bertrand N Dozois CM Harel J 《Veterinary microbiology》2011,153(1-2):82-88
During the course of infection, bacteria must coordinately regulate gene expression in response to environmental stimuli. The phosphate (Pho) regulon is controlled by the two component-regulatory system PhoBR. PhoBR is activated during starvation and regulates genes involved in phosphate homeostasis. Several studies have highlighted the importance of the Pho regulon in bacterial pathogenesis, showing how induction of PhoBR, in addition to regulating genes participating in phosphate metabolism, leads to modulation of many cellular processes. The pleiotropic effects of Pho regulon activation include attenuated virulence and alteration of many virulence traits, including adhesion to host cells and resistance to cationic antimicrobial peptides, acidity and oxidative stresses. This review provides an overview of the relationship between the Pho regulon and virulence in Escherichia coli and illustrates that, in addition to regulating phosphate homeostasis, the Pho regulon plays a key role in regulating stress responses and virulence. 相似文献
76.
Petrović Andjeljko Pérez Hidalgo Nicolas Michelena Saval José Manuel Tomanović Željko 《Phytoparasitica》2021,49(1):93-101
Phytoparasitica - Here we describe Aphidius erodii sp. n. associated with Acyrthosiphon malvae (Mosley, 1841), which is a pest on herbaceous ornamental Geranium and Pelargonium plants, but also an... 相似文献
77.
Cambou Aurélie Saby Nicolas P.A. Hunault Gilles Nold François Cannavo Patrice Schwartz Christophe Vidal-Beaudet Laure 《Journal of Soils and Sediments》2021,21(2):1038-1052
Journal of Soils and Sediments - Assessments of urban soil organic carbon stock (SOCS) are scant because citywide data are scarce. Moreover, we do not know which factors mostly explain SOCS spatial... 相似文献
78.
Nicolas Tremblay Zhijie Wang Bao-Luo Ma Carl Belec Philippe Vigneault 《Precision Agriculture》2009,10(2):145-161
Nitrogen (N) fertilizer rates applied spatially according to crop requirements can improve the efficiency of N use. The study
compares the performance of two commercial sensors, the Yara N-Sensor/FieldScan (Yara International ASA, Germany) and the
GreenSeeker (NTech Industries Inc., Ukiah, California, USA), for assessing the status of N in spring wheat (Triticum aestivum L.) and corn (Zea mays L.). Four experiments were conducted at different locations in Quebec and Ontario, Canada. The normalized difference vegetation
index (NDVI) was determined with the two sensors at specific growth stages. The NDVI values derived from Yara N-Sensor/FieldScan
correlated with those from GreenSeeker, but only at the early growth stages, where the NDVI values varied from 0.2 to 0.6.
Both sensors were capable of describing the N condition of the crop or variation in the stand, but each sensor had its own
sensitivity characteristics. It follows that the algorithms developed with one sensor for variable-rate N application cannot
be transferred directly to another sensor. The Yara N-Sensor/FieldScan views the crop at an oblique angle over the rows and
detects more biomass per unit of soil surface compared to the GreenSeeker with its nadir (top-down) view of the crop. The
Yara N-Sensor/FieldScan should be used before growth stage V5 for corn during the season if NDVI is used to derive crop N
requirements. GreenSeeker performed well where NDVI values were >0.5. However, unlike GreenSeeker, the Yara N-Sensor/FieldScan
can also record spectral information from wavebands other than red and near infrared, and more vegetation indices can be derived
that might relate better to N status than NDVI. 相似文献
79.
In many countries, including the European Union member states, Switzerland, Australia, New Zealand, and Japan, legislation has been set up for labeling of genetically modified organisms (GMOs) in food and feed products. To comply with these regulations, reliable detection methods are necessary. If the detection is based on DNA, a GMO analysis may contain several steps where qualitative and quantitative species-specific, GMO screening, GMO construct, and GMO line-specific polymerase chain reactions (PCRs) are used. A limit of detection (LOD) thereby defines to what extent a target molecule may be detected in a sample. In this study, cookies were made with variable levels of a soy sample containing 2 wt% Roundup Ready soy. For all PCRs described, detection limits based on dilution series and practical LODs were determined. The practical LODs are used to determine to what extent a GMO ingredient may be detected in a real food product. Results reveal that, due to the baking process, the overall DNA fragment length is reduced, rendering GMO analyses more difficult. Furthermore, Roundup Ready soy line-specific and real-time quantitative PCR are less sensitive than GMO screening PCRs, whereas just these PCRs are crucial in the decision-making process regarding the presence of GMOs in a food product. Moreover, high standard deviations and errors render the precise quantification of GMOs difficult. 相似文献
80.
ABSTRACT European stone fruit yellows (ESFY) is becoming a major economic problem for Prunus growers in Europe. The causal agent ("Candidatus Phytoplasma prunorum") and its vector (Cacopsylla pruni) have been identified, but the present knowledge of the risk factors for this disease relies, at best, on specific experiments. To assess the relative significance of several factors correlated with ESFY incidence in the field, an exhaustive survey was performed on apricot and Japanese plum orchards in the Crau plain (France). After a preliminary multivariate exploration of the data, we used a logistic regression model to analyze and predict the cumulative number of diseased trees on the basis of a set of quantitative (age, planting density, and area of the orchard) and categorical variables (species, cultivar, and rootstock). Because of the nature of the data, we used an overdispersed binomial model and we developed a parametric bootstrap procedure based on the beta-binomial distribution to obtain confidence intervals. Our results indicated that the age, species, and cultivar of the scion were the major factors explaining the observed number of diseased trees. The planting density and the rootstocks used in the zone under study were less significant, and the area of the orchard had no effect. The residuals of the model showed that some explanatory variables had not been taken into account, because part of the remaining variability could be explained by a grower effect. The spatial distribution of the residuals suggested that one of the reasons for this grower effect was the correlation between orchards closer than 100 m, possibly caused by the flight behavior of infectious vectors. 相似文献