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11.
Nick A Ciccone William Mwangi Alexey Ruzov Lorraine P Smith Colin Butter Venugopal Nair 《Veterinary research》2014,45(1)
The mechanisms by which viruses modulate the immune system include changes in host genomic methylation. 5-hydroxymethylcytosine (5hmC) is the catalytic product of the Tet (Ten-11 translocation) family of enzymes and may serve as an intermediate of DNA demethylation. Recent reports suggest that 5hmC may confer consequences on cellular events including the pathogenesis of disease; in order to explore this possibility further we investigated both 5-methylcytosine (5mC) and 5hmC levels in healthy and diseased chicken bursas of Fabricius. We discovered that embryonic B-cells have high 5mC content while 5hmC decreases during bursa development. We propose that a high 5mC level protects from the mutagenic activity of the B-cell antibody diversifying enzyme activation induced deaminase (AID). In support of this view, AID mRNA increases significantly within the developing bursa from embryonic to post hatch stages while mRNAs that encode Tet family members 1 and 2 reduce over the same period. Moreover, our data revealed that infectious bursal disease virus (IBDV) disrupts this genomic methylation pattern causing a global increase in 5hmC levels in a mechanism that may involve increased Tet 1 and 2 mRNAs. To our knowledge this is the first time that a viral infection has been observed to cause global increases in genomic 5hmC within infected host tissues, underlining a mechanism that may involve the induction of B-cell genomic instability and cell death to facilitate viral egress. 相似文献
12.
Stephen George David Burgess Michael Leaver Nick Frerichs 《Fish physiology and biochemistry》1992,10(1):43-54
Intraperitoneal injection of turbot with Cd induced the synthesis of a low molecular weight hepatic Cd-binding protein and a 500bp mRNA, which hybridised to a plaice metallothionein (MT) cRNA probe. The Cd-binding protein displayed cross-reactivity in a competitive ELISA with antiserum raised against rainbow trout MT and had the characteristic amino acid composition, metal stoichiometry and spectral characteristics of a Class I MT. Only one isoform was apparent on ion exchange chromatography. Southern blot analysis of DNA cleaved with four restriction enzymes suggested that only a single MT gene is present in turbot.In an established turbot fibroblast cell line, Cd induced MT mRNA and MT levels in a dose and time-dependent manner. MT was also induced by Cu, Hg and Zn but not Pb exposure. Physiological concentrations of glucocorticoids and sex hormones did not induce MT synthesis, although at high concentrations a positive response to corticosterone, dexamethasone, hydrocortisone or progesterone was observedin vitro indicating the possible presence of a functional steroid regulatory element in the fish MT gene.Abbreviations used AMP
adenosine monophosphate
- CHSE
chinook salmon embryo
- DMSO
dimethyl sulphoxide
- HPLC
high performance liquid chromatography
- KB
kenacid blue
- MT
metallothionein
- Mr
molecular weight (kDaltons)
- NR
neutral red
- PEG
polyethylene glycol
- RTH
rainbow trout hepatoma
- SDS
sodium dodecyl sulphate
- SSC
0.15M NaCl, 0.015M sodium citrate
- TF
turbot fibroblast 相似文献
13.
14.
Agent-based land-use models: a review of applications 总被引:9,自引:0,他引:9
Robin B. Matthews Nigel G. Gilbert Alan Roach J. Gary Polhill Nick M. Gotts 《Landscape Ecology》2007,22(10):1447-1459
Agent-based modelling is an approach that has been receiving attention by the land use modelling community in recent years,
mainly because it offers a way of incorporating the influence of human decision-making on land use in a mechanistic, formal,
and spatially explicit way, taking into account social interaction, adaptation, and decision-making at different levels. Specific
advantages of agent-based models include their ability to model individual decision-making entities and their interactions,
to incorporate social processes and non-monetary influences on decision-making, and to dynamically link social and environmental
processes. A number of such models are now beginning to appear—it is timely, therefore, to review the uses to which agent-based
land use models have been put so far, and to discuss some of the relevant lessons learnt, also drawing on those from other
areas of simulation modelling, in relation to future applications. In this paper, we review applications of agent-based land
use models under the headings of (a) policy analysis and planning, (b) participatory modelling, (c) explaining spatial patterns
of land use or settlement, (d) testing social science concepts and (e) explaining land use functions. The greatest use of
such models so far has been by the research community as tools for organising knowledge from empirical studies, and for exploring
theoretical aspects of particular systems. However, there is a need to demonstrate that such models are able to solve problems
in the real world better than traditional modelling approaches. It is concluded that in terms of decision support, agent-based
land-use models are probably more useful as research tools to develop an underlying knowledge base which can then be developed
together with end-users into simple rules-of-thumb, rather than as operational decision support tools.
This paper arises from research conducted as part of the UK Research Councils’ RELU Programme (award number RES-224-25-0102).
RELU is funded jointly by the Economic and Social Research Council, the Biotechnology and Biological Sciences Research Council
and the Natural Environment Research Council, with additional funding from the Department for Environment, Food and Rural
Affairs and the Scottish Executive Environment and Rural Affairs Department. 相似文献
15.
The causes of clinical signs associated with syringomyelia in the Cavalier King Charles Spaniel (CKCS) are incompletely understood. In this study we compared expression of two pain-related neuropeptides: substance P (SP) and calcitonin gene related peptide (CGRP), in the spinal cord dorsal horn of normal dogs with that in CKCS with and without clinical signs of syringomyelia. There was a decrease in expression of both peptides in CKCS with 'symptomatic' syringomyelia that was also associated with significant asymmetry in SP-I and similar, though non-significant, asymmetry in CGRP-I compared with other groups. The asymmetric distribution of these pain-related peptides may be a consequence of syrinx-associated damage to grey matter but may also play a role in generation of pain. 相似文献
16.
Abstract: This paper contributes political and cultural‐economy perspectives to the critique of the MIRAB model 20 years on. In it, we celebrate the politically grounded reading by MIRAB analysts of development in the small island nations of the Pacific and their attention to both the empirical and the structural in their treatment of the economies of these countries. We address aspects, however, of one of the common critiques of MIRAB analyses: their failure to capture accurately the nature of small island socio‐cultural economies. We focus on the workings of remittance systems on two of the Cook Islands, Mauke and Manihiki, as the basis for a more thorough critique. We argue that rather than living economically and nationally determined lives, Cook Islanders live in rich networks of flows of goods, people, labour and meaning that the MIRAB model does not fully capture. The microeconomics of the transnational kin or household unit and the remittance decision are deeply embedded in such networks. These networks generate their own, temporary constellations of responsibility, economy and decision‐making, which may or may not materialise at any point as household economy. We consider some of the consequences of a network view for MIRAB analyses and for development in small island nations. 相似文献
17.
Sarah A. Bekessy Brendan A. Wintle Ascelin Gordon Julian C. Fox Ryan Chisholm Bill Brown Tracey Regan Nick Mooney Steve M. Read Mark A. Burgman 《Biological conservation》2009,142(11):2438-2448
The wedge-tailed eagle is Australia’s largest bird of prey and one of the largest eagles in the world. Aquila audax fleayi is an endemic Tasmanian subspecies isolated for 10,000 years from the nominate subspecies on the Australian mainland. The Tasmanian wedge-tailed eagle is classified nationally and at a State level as endangered due to its small number of breeding pairs, low breeding success and high rate of mortality from unnatural causes. The subspecies experiences mortality throughout its range from shooting, poisoning, trapping, road accidents, electrocutions and collisions with wind turbines, aircraft, fences and overhead wires, which we term ‘un-natural mortality’. A portion of the subspecies’ range is managed for timber production, which can lead to disturbance of nest sites and the loss of nest trees. We use a model of the eagle population from the Bass District in northeast Tasmania to explore the relative importance of different sources of mortality and nesting habitat loss, and the potential for mitigating impacts associated with unnatural mortality, disturbance, nesting habitat loss and human access to forests. We create a habitat map including suitable nest sites and link it to a dynamic landscape population model based on life history traits and disturbance responses. Using the program RAMAS-Landscape, we model alternative forest management scenarios, ranging from no timber harvesting and a natural wildfire regime, to scenarios prescribing native forest harvesting and regeneration and different levels of conversion of native forest to plantation under the same natural wildfire regime. The results indicate that the Tasmanian wedge-tailed eagle is sensitive to unnatural mortality, plantation establishment and native forest harvesting. The predicted decline over the next 160 years (65%) will most likely be driven largely by loss of current and potential future nest sites associated with harvesting activities, exacerbated by unnatural mortality in the wider landscape. Interventions that minimise unnatural mortality, reduce nest disturbance, and retain breeding habitat and nest sites may improve the prospects for the subspecies in the Bass District. If nest disturbance and unnatural mortality continue at the rates modelled here, the species appears to face a high risk of declining substantially in the region. 相似文献
18.
Purpose
Limited information exists about the zinc (Zn) status of major soils in northeast China, and their ability to supply Zn to soybeans. This study aims to establish the critical concentrations of Zn in soil for plant growth, to characterize the Zn forms in soil, to correlate the concentration of various Zn forms with Zn uptake by soybeans, and to identify the most effective means of Zn application. 相似文献19.
A survey of forestry practices and attitudes was undertaken in four communities in Leyte, the Philippines, to improve understanding
of the social and economic factors affecting small-scale forestry development. The survey had three main data collection activities
— initial focus group discussions (FGDs), household interviews, and reporting and validation FGDs. A team of enumerators was
selected for household interviews which consisted of both males and females, to avoid potential problems of unwillingness
of people to talk with those of the opposite gender. The interviewers were also required to be able to speak local dialects
(Cebuano and Waray Waray), the survey questionnaires being administered in these dialects. Various methods were used to gain
the support and assistance of local government units and barangay captains. Some difficulty was experienced by the survey
team in the first community due to barangay elections at the time of the survey, and the requirement by the University of
Queensland Ethics Committee that respondents sign a consent form. This requirement was found to be not culturally appropriate
for the Leyte smallholder communities. Offering goods at the end of the interview was found to be of limited value for encouraging
participation in the survey. Provision of food and drinks were found to encourage FGD participants to express their views,
but too much alcohol had a negative effect. The importance of providing comprehensive feedback to respondents and involving
them and other stakeholders in development of policy recommendations was apparent. These survey experiences provide valuable
insights which are not generally available in textbooks on sample surveys, and provide lessons for planning and conducting
smallholder community survey into natural resource management issues. 相似文献
20.
Kai S. J. Lewis Amanda Black Leo M. Condron Nick W. Waipara Peter Scott Nari Williams Maureen OCallaghan 《Forest Pathology》2019,49(2)
Phytophthora agathidicida is the accepted causal agent of dieback in remnant stands of long‐lived indigenous New Zealand kauri (Agathis australis) and poses a significant threat to the long‐term survival of this species. Little is known about the effect of key soil physicochemical characteristics on the growth of P. agathidicida. In this study, we investigated the growth of P. agathidicida in soils collected from adjacent areas under original kauri forest, short rotation pine (Pinus radiata) plantation forest and grazed pastures. A growth response assay was used to quantify asexual (sporangia) and sexual (oospore) spore counts over 8 days in soils sampled from each land‐use. Significantly higher numbers of sporangia (p < 0.001) and oospores (p < 0.01) were found in pasture and pine forest soil within 2 days of the growth assay trials, suggesting these soils may favour asexual/sexual reproduction in the early stages of P. agathidicida establishment compared to kauri forest soils. Additionally, oospore production significantly increased over 8 days in pine forest soil, suggesting that with an increase in inoculum loads, these soils potentially act as pathogen reservoirs. The soil physicochemical properties (e.g., pH, C and N, phosphorus content and electrical conductivity) investigated in this study did not significantly correspond to spore count data between land‐uses, suggesting that differences in growth response are driven by other edaphic factors not explored in the present study. 相似文献