棉花单核苷酸多态性标记研究进展

单核苷酸多态性标记已在农作物研究中得到广泛应用并取得重大进展。为了便利棉花SNP(Single nucleotide polymorphism)标记的研究和应用,介绍了利用基因芯片、简化基因组测序、重测序等在棉花中开发SNP标记的方法 ,综述了SNP标记在棉花遗传图谱构建、数量位点的定位和分子标记辅助育种、基因组测序以及系统进化等研究中的应用。并对异源四倍体棉花中SNP标记开发时,同源序列位点和部分同源序列位点上的SNP标记辨别问题进行了系统探讨,对其快捷的开发、检测方式和在数量基因定位中的应用前景进行了展望。     

农业现代化过程中我国农机合作社的特点、困境与对策

我国正处在从传统农业向现代农业转变的关键阶段.高度的农业机械化是现代农业的一个重要特征.我国农机作业合作社发展迅速,积累了大量的成功经验,但是也面临着一些问题,需要从加强对农机作业合作社人员的教育与培训、增加农机作业合作社的规模化效益、完善农机作业合作社内部运行机制和政府营造良好的外部环境等方面予以解决.     

猪的福利与放牧养猪

通过对动物福利的概况阐述,分析了我国猪饲养环境中存在的动物福利问题以及放牧养猪对猪只福利的影响,提出了发展放牧养猪的思路。     

Effect of ammonia and nitrite on vigour,survival rate,moulting rate of the blue swimming crab <Emphasis Type="Italic">Portunus pelagicus</Emphasis> zoea

The effects of ammonia and nitrite on vigour, survival rate, moulting rate of zoea of blue swimming crab, Portunus pelagicus, were studied. A total of five nitrite-N treatments (26.67, 53.34, 106.68, 213.36, 426.72 mg/l) and a control (no nitrite-N added) were set up for the acute nitrite-N toxicity experiment; a total of five ammonia-N treatments (8.43, 16.86, 33.72, 67.44, 134.88 mg/l) and a control (no ammonia-N added) were set up for the acute ammonia-N toxicity experiment. The results showed that the vigour, survival rate and moulting rate of zoea of the blue swimming crabs exposed to over 53.34 mg/l were significantly different (P < 0.05) from the control group. The zoea LC₅₀ values (mg/l) of nitrite-N were 179.47, 76.56, 66.70, 37.49, 25.01, 25.35, 25.34 mg/l for 24, 36, 48, 60, 72, 84, 96 h, respectively. The vigour, survival rate and moulting rate of zoea of the blue swimming crabs exposed to over 16.86 mg/l were significantly different (P < 0.05) from the control group. The zoea LC₅₀ values (mg/l) of ammonia-N were 51.04, 39.62, 38.72, 24.43, 16.90, 13.42, 11.16 mg/l for 24, 36, 48, 60, 72, 84, 96 h, respectively. The zoeae are highly sensitive to ammonia and nitrite, and the toxicity of ammonia and nitrite on Portunus pelagicus decrease with development of this crab.     

我国开征房产税的可行性研究

随着房价的居高不下，房产税的征收在近期已成为热门话题。通过研究房产税开征的情况，追溯房产税的历史起源，考察征收房产税的原因，以及分析在现时的中国征收房产税的特殊情况，并综合运用比较法、例证法等各种方法，以期在众说纷纭中，得到一个清醒的认识，并最终说明房产税征收的时机并不成熟，现在的征收热还只是国家的造势之举     

玉米DDGS中抗生素残留的潜在风险及其检测方法

本文就DDGS产品中抗生素残留风险及其检测方法作一综述。     

Evaluation of the colonization capabilities of Salmonella Enteritidis in Quails using an RT-PCR approach

We used a real-time PCR assay and indirect fluorescent antibody (IFA) assay to detect genomic DNA of Salmonella Enteritidis in the internal organs of quails after an oral challenge. The results showed that S. Enteritidis was detected in all the samples at different time points. This study will assist a future understanding of the pathogenesis of S. Enteritidis.     

论房屋征收与补偿过程中的维权机制

随着现阶段城市建设速度的加快，各地因拆迁行为引发的争议越来越激烈。为了更好地协调城市发展与私人权益的关系，2011年1月21日国务院颁布了《国有土地上房屋征收与补偿条例》（简称《征收条例》，下同）。与先前的《城市房屋拆迁管理条例》相比较，《征收条例》无论在立法的价值目标、具体的实施机制方面都有很大的区别。以《征收条例》和相关立法作为制度框架，构建并逐步完善房屋征收与补偿过程中的维权机制，有助于使得包括《征收条例》在内的上述立法得以贯彻实现，从而使得利益博弈在市场经济、法治国家的轨道上进行。     

噻苯隆(TDZ)在红掌组织培养中的应用

研究了噻苯隆(TDZ)在红掌外植体诱导及增殖继代过程中的作用。结果表明,在外植体诱导阶段,添加0.1mg·L-1的TDZ,可以缩短诱导时间,提高诱导率。TDZ浓度大于0.1mg·L-1会导致愈伤组织和分化芽的畸形发展,而且会影响红掌组培苗移栽之后的长势。在愈伤增殖阶段,TDZ的使用没有明显的促进作用。     

Dietary fiber in a low-protein diet during gestation affects nitrogen excretion in primiparous gilts,with possible influences from the gut microbiota

We investigated the effects of dietary fiber (DF) supplementation in normal or low crude protein (CP) diets on reproductive performance and nitrogen (N) utilization in primiparous gilts. In total, 77 Landrace × Yorkshire pregnant gilts were randomly allocated to four dietary treatments in a 2 × 2 factorial design. The groups comprised 1) equal intake of normal CP (12.82% and 0.61% total lysine), 2) low CP (LP) (10.53% and 0.61% total lysine), and 3) with or 4) without DF supplementation (cellulose, inulin, and pectin in a 34:10:1 ratio). A low-protein diet during gestation significantly reduced daily weight gain from days 91 to 110 of pregnancy (−162.5 g/d, P = 0.004). From N balance trials conducted at days 35 to 38, 65 to 68, and 95 to 98 of pregnancy, DF addition increased fecal N excretion at days 65 to 68 (+24.1%) and 95 to 98 (+13.8%) of pregnancy (P < 0.05) but reduced urinary N excretion (P < 0.05), resulting in greater N retention at each gestational stage. DF increased fecal microbial protein levels and excretion during gestation. An LP diet also reduced urinary N excretion at different gestational stages. An in vitro fermentation trial on culture media with nonprotein N urea and ammonium bicarbonate (NH₄HCO₃) as the only N sources revealed that microbiota derived from feces of gestating gilts fed the high DF diet exhibited a greater capacity to convert nonprotein N to microbial protein. Microbial fecal diversity, as measured by 16S rRNA sequencing, revealed significant changes from DF but not CP diets. Gilts fed an LP diet had a higher number of stillbirths (+0.83 per litter, P = 0.046) and a lower piglet birth weight (1.52 vs. 1.37 kg, P = 0.006), regardless of DF levels. Collectively, DF supplementation to gestation diets shifted N excretion from urine to feces in the form of microbial protein, suggesting that the microbiota had a putative role in controlling N utilization from DF. Additionally, a low-protein diet during gestation negatively affected the litter performance of gilts.