Donors for Resistance to Brown Planthopper Nilaparvata lugens (Stål) from Wild Rice Species

Out of 1 989 wild accessions sown in seed boxes for screening, only 1 003 wild accessions with good germination were screened against brown planthopper (BPH), Nilaparvata lugens (Stål) under greenhouse conditions. The collection comprised of accessions from 11 wild species and African cultivated rice. The germplasm was screened for BPH following standard seed box screening technique in the greenhouse. As many as 159 accessions were identified as resistant during the year 2012 based on one year screening. A selected set of BPH resistant accessions were screened again during 2013. Based on the two years screening, seven accessions of O. nivara (AA), one accession of O. officinalis (CC), seven accessions of O. australiensis (EE), five accessions of O. punctata (BB and BBCC) and nine accessions of O. latifolia (CCDD) were confirmed to be resistant to BPH. So far no BPH resistance genes have been identified and designated from O. nivara and O. punctata, hence these may act as new sources of resistance.     

Response of spring wheat to boron-coated urea and its effect on nitrogen use efficiency

A field study was conducted at the ICAR-Indian Agricultural Research Institute, New Delhi, to study the response of spring wheat to boron applied as boron-coated urea (BCU) and the effect of boron (B) coating on the use efficiency of applied fertilizer urea nitrogen. The BCU (0.5% B) significantlyrecorded the highest increase in leaf area index (30.2%), spike length (12.5%), number of spikes (10.9%), filled grains (15.7%) and grain weight (16.3%) per spike in spring wheat than uncoated prilled urea (PU). Furthermore, the application of 0.5% BCU enhanced the grain and straw yields of spring wheat by 11% and 10.6% over uncoated PU. The BCU (0.5% B) also significantly increased the N concentration and N uptake both in wheat grain and straw. The BCU (0.5% B) recorded 24.8% higher recovery efficiency of applied nitrogen and an increase of 3.6 kg grain kg^?1 N applied in agronomic efficiency compared to uncoated PU. The net returns (USD915.1 ha^?1) and benefit:cost ratio (1.40) werealso the highest with 0.5% BCU, significantly higher than uncoated PU. Therefore, 0.5% boron coating onto PU is recommended for increased productivity and enhanced efficiency of applied fertilizer nitrogen for spring wheat in Indo-Gangetic plains of India.     

Mapping of easy to screen SSR markers for selection of RFLP markers-bracketed downy mildew resistance QTLs in pearl millet

There is a growing necessity to replace chemical agents with ecofriendly materials, arising from the impact on the environment and/or human health, which calls for the design of new broad-spectrum fungicides. In this work, chitosan oligomers (COs), propolis (Ps) and silver nanoparticles (AgNPs) mixtures in solution were assessed to control the growth of different phytopathogenic fungi and oomycetes in vitro. Binary solutions of COs-Ps and COs-AgNPs evinced the highest antifungal effect against Fusarium circinatum and Diplodia pinea fungi, respectively, with a ca. 80% reduction in their mycelial growth. The COs solution by itself also proved to be greatly effective against Gremmeniella abietina, Cryphonectria parasitica and Heterobasidion annosum fungi, causing a reduction of 78%, 86% and 93% in their growth rate, respectively. Likewise, COs also attained a 100% growth inhibition on the oomycete Phytophthora cambivora. On the other hand, Ps inhibited totally the growth of Phytophthora ×alni and Phytophthora plurivora. The application of AgNPs reduced the mycelial growth of F. circinatum and D. pinea. However, the AgNPs in some binary and ternary mixtures had a counter-productive effect on the anti-fungal/oomycete activity. In spite of the fact that the anti-fungal/oomycete activity of the different treatments showed a dependence on the particular type of microorganism, these solutions based on natural compounds can be deemed as a promising tool for control of tree diseases.     

Immune responses to the capsular polysaccharide of Mycoplasma dispar in calves and mice

Humoral and cell-mediated immune responses to the capsular polysaccharide (CPS) of M. dispar and polygalacturonic acid (pGaIU—a structurally similar polysaccharide) were investigated in calves experimentally infected with Mycoplasma dispar and in mice immunized with CPS or pGaIU. Sera, tracheobronchial lavage and nasal fluids, collected before and after infection in calves, were checked for the presence of anti-CPS and anti-pGaIU antibodies. The sera from mice injected with CPS or pGaIU were checked for different classes of anti-CPS and anti-pGaIU antibodies. Peripheral blood lymphocytes from calves and splenic lymphocytes from mice were monitored for specific proliferative responses to CPS and pGaIU. At about 2 weeks post-infection, anti-CPS IgM response in serum, anti-CPS and anti-pGaIU IgM and IgA response in lavage fluid and lymphocyte proliferative response was seen in the calves. Mice immunized with CPS and pGaIU gave exclusively IgM responses. No secondary response was seen in mice immunized with CPS in contrast to mice immunized with pGaIU. Antibodies cross-reactive with pGaIU were present in the sera of CPS-immunized mice but antibodies cross-reactive with CPS were not found in pGaIU-immunized mice. No significant blastogenic response was shown by mouse splenocytes to CPS or pGaIU.     

Chemoprophylaxis of Anaplasma ovis infection in sheep with a long-acting oxytetracycline

The chemoprophylactic efficacy of long-acting oxytetracycline was determined in 13 susceptible ewes infected with Anaplasma ovis. The drug was administered intramuscularly at a dose rate of 20 mg kg-1 body weight during the prepatent period. When the ewes were exposed to an equivalent homologous challenge on Day 45 post-infection, each showed a mild or inapparent reaction. The host reactions, i.e., body temperature, parasitaemia, packed-cell volume (PCV), haemoglobin, humoral and cell mediated immunity (CMI) were also studied. The results indicate that the use of oxytetracycline during the incubation period would minimise clinical signs of an Anaplasma infection; this may be due to an increasing CMI response.     

Pharmacokinetic Profile of Erythromycin after Intramammary Administration in Lactating Dairy Cows with Specific Mastitis

The pharmacokinetics of erythromycin was studied in five lactating dairy cows following single intramammary infusion of 300 mg erythromycin in each of two quarters per cow with specific mastitis. Levels of erythromycin in plasma and quarter milk samples were measured by agar plate diffusion assay using Micrococcus luteus (ATCC 9341) as the test organism. Erythromycin level in plasma reached a peak concentration value (C _max) of 0.07 ± 0.01 μg/ml at 30 min; thereafter, levels declined gradually to reach 0.05 ± 0.00 μg/ml 12 h post drug administration. The pharmacokinetic profile of the drug revealed mean absorption half life (t _1/2ka) as 0.26 ± 0.05 h. The drug was eliminated slowly with elimination half-life (t _1/2β) of 13.75 ± 0.35 h and elimination rate constant (k _el) of 0.04 ± 0.00 h⁻¹. The volume of distribution based on the zero-time plasma concentration intercept of the least-squares regression line of the elimination phase (V _d(B)) was 0.032 L/kg. The drug crossed to untreated quarters also; mean drug levels of 0.20 ± 0.07, 0.23 ± 0.07, 0.17 ± 0.04, and 0.17 ± 0.04 μg/ml were found at 3, 6, 8 and 12 h, respectively. The mean drug concentration for treated quarters was measured as 22.97 ± 2.31 μg/ml milk at first milking (12 h) following drug infusion. No apparent adverse reaction was seen in cows administered erythromycin. It is concluded that following intramammary infusion erythromycin diffuses readily and extensively in various body fluids and tissues and adequate concentration is maintained in udder tissues for at least 12 h post intramammary administration. Thus, erythromycin may be recommended for local therapy of acute mastitis caused by Gram-positive bacteria in lactating dairy cows.     

Effect of subsurface drip irrigation on onion yield

Subsurface drip system is the latest method of irrigation. The design of subsurface drip system involves consideration of structure and texture of soil, and crop’s root development pattern. A 3-year experiment was conducted on onion (Allium Cepa L., cv. Creole Red) in a sandy loam soil from October to May in 2002–2003, 2003–2004 and 2004–2005 to study the effect of depth of placement of drip lateral and different levels of irrigation on yield. Tests for uniformity of water application through the system were carried out in December of each year. Three different irrigation levels of 60, 80 and 100% of the crop evapotranspiration and six placement depths of the drip laterals (surface (0), 5, 10, 15, 20 and 30 cm) were maintained in the study. Onion yield was significantly affected by the placement depth of the drip lateral. Maximum yield (25.7 t ha⁻¹) was obtained by applying the 60.7 cm of irrigation water and by placing the drip lateral at 10 cm soil depth. Maximum irrigation water use efficiency (IWUE) (0.55 t ha⁻¹ cm⁻¹) was obtained by placing the drip lateral at 10 cm depth. The greater vertical movement of water in the sandy-loam soil took place because of the predominant role of gravity rather than that of the capillary forces. Therefore, placement of drip lateral at shallow depths is recommended in onion crop to get higher yield.