熏蒸剂的使用受限促进气调技术的发展

熏蒸药剂残留对食品、环境的危害问题使得相关标准制定单位采取措施严格限制熏蒸剂的使用.二十年前曾经使用的多数熏蒸剂如今已所剩无几.甲基溴具有较高效的杀虫效果,但是由于它对臭氧层的破坏严重,发达国家已于2005年淘汰使用,发展中国家也将于2015年之前淘汰甲基溴.比较而言,现今使用普遍的仍是磷化氢,然而近年来害虫对磷化氢的抗性问题显得日益严重.这些熏蒸药剂的种种局限,已经向食品企业提出了新的全球性的挑战,要求致力于寻找新的熏蒸剂,开发新的替代技术.目前,被提及的新的熏蒸剂有硫酰氟、氧硫化碳(carbonyl sulphide)、环氧丙烷(propylene oxide)、甲基碘(methyl jodide)、臭氧(ozone)、甲酸甲酯(ethyl formate)和氢氰酸(hydrogen cyanide).硫酰氟被认为是很有前景的熏蒸剂替代品,可用来有效地保护储藏食品、食品加工设备免受害虫危害,而且还可以作为一种检疫用的熏蒸剂.其它一些新开发的熏蒸剂由于只对特定种类的储藏物品有效或在特定条件下才有效,所以使用的局限性很大.试验证明:具有挥发性的植物提取剂有较好的使用前景,但还需要大量的商业生产验证试验,而且在投入生产实践之前还需要一系列的认证程序.可成功替代熏蒸药剂的众多气体处理技术有:气调技术、高温结合气调技术以及进一步开发用作特定用途的高压二氧化碳技术.当前研究开发的是低压环境下的气调技术.这些研究证明气调技术很有市场前景,应当作为全球开发的新方法的一个典范.     

Topographical surveying for improved assessment of sediment retention in check dams applied to a Mediterranean badlands restoration site (Central Spain)

Purpose

The effects of check dams used in restoration projects have been discussed in a number of papers in recent years. This paper studies the effectiveness of retaining sediments from check dams constructed in the badlands restoration site of Tórtoles, located in the Corneja River basin (Spain), using a new topographical method.

Materials and methods

In order to assess the sediment-retaining capacity of the check dams, we measured the volume of sediment trapped by 15 of the 123 check dams built in 1965. We carried out a detailed topographical survey using a Total Station, with an accuracy of ±1 cm, to measure cross sections of the sediment trapped by each check dam. The results were then compared with those of two simplified methods which consider the volume of retained sediment as a simple geometric figure.

Results and discussion

According to our results, 258 m³ of sediment has been retained by the check dams. These results show a significant discrepancy between the topographical method and the two other methods, whose values are consistently lower (14 to 20 %). According to our survey, the mean value for the volume of sediment retained by each check dam is 17.23 m³, versus 13.86 and 14.74 m³ when applying the other methods. Although there is a strong correlation between the volume of retained sediment computed by the topographical method and the other two methods (r ² from 0.96 to 0.94), the differences between them increase with the increasing size of the check dams. Therefore, total differences are expected to be more significant with larger check dams. The erosion rates, calculated on the basis of the retained sediment in the Tórtoles check dams, are 16–21 % lower when using the simpler methods. The bed slopes of the streams were reduced 12.44 % because of the presence of the check dams.

Conclusions

After having completed a more detailed topographical survey of the sediment trapped in the check dams, our results are more likely to estimate erosion rates and sediment yield correctly, thus leading to a better understanding of the effects of check dams on badlands restoration.     

Identification and Selection for Tuber Calcium,Internal Quality and Pitted Scab in Segregating ‘Atlantic’ x ‘Superior’ Reciprocal Tetraploid Populations

Developing chipping cultivars with improved tuber quality and disease resistance is a major interest for breeders and the potato industry. A popular chipping cultivar ‘Atlantic’, is desired for its high yield and gravity. However, this cultivar suffers from poor internal tuber quality and high scab susceptibility. On the contrary, cultivar ‘Superior’ is known to have excellent tuber internal quality and moderately scab resistance. In addition, this cultivar is known to have high tuber calcium as compared to ‘Atlantic’. The present study intended to generate populations that can be suitable for the genetic study of tuber calcium, internal quality, common scab, and other commercially important traits such as yield, specific gravity and chip quality at the tetraploid level. Two populations obtained by reciprocally crossing the cultivars ‘Atlantic’ and ‘Superior’ were evaluated during 2009 to 2012 at Hancock, Wisconsin. Significant genotype effects and moderately low to high broad-sense heritabilities were identified for all traits evaluated indicating that the observed phenotypic variation has an important genetic component. In addition, the parents differed significantly for all traits across trials, and most genotypes performed in between the two parents but some genotypes were more extreme than the parents. Furthermore, evidence of reciprocal effects was found for some traits. In addition to learning about the genetics of these important traits we were able to identify some genotypes that combined the commercially desired traits of the two cultivars.     

Anthelmintic activity of Cocos nucifera L. against sheep gastrointestinal nematodes

The development of anthelmintic resistance has made the search for alternatives to control gastrointestinal nematodes of small ruminants imperative. Among these alternatives are several medicinal plants traditionally used as anthelmintics. This work evaluated the efficacy of Cocos nucifera fruit on sheep gastrointestinal parasites. The ethyl acetate extract obtained from the liquid of green coconut husk fiber (LGCHF) was submitted to in vitro and in vivo tests. The in vitro assay was based on egg hatching (EHT) and larval development tests (LDT) with Haemonchus contortus. The concentrations tested in the EHT were 0.31, 0.62, 1.25, 2.5 and 5 mg ml⁻¹, while in the LDT they were 5, 10, 20, 40 and 80 mg ml⁻¹. The in vivo assay was a controlled test. In this experiment, 18 sheep infected with gastrointestinal nematodes were divided into three groups (n = 6), with the following doses administered: G1—400 mg kg⁻¹ LGCHF ethyl acetate extract, G2—0.2 mg kg⁻¹ moxidectin (Cydectin^®) and G3—3% DMSO. The worm burden was analyzed. The results of the in vitro and in vivo tests were submitted to ANOVA and analyzed by the Tukey and Kruskal–Wallis tests, respectively. The extract efficacy in the EHT and LDT, at the highest concentrations tested, was 100% on egg hatching and 99.77% on larval development. The parameters evaluated in the controlled test were not statistically different, showing that despite the significant results of the in vitro tests, the LGCHF ethyl acetate extract showed no activity against sheep gastrointestinal nematodes.     

Estimation of soil salinity in semi‐arid land using a geostatistical model

Soil salinity is one of the great problems in arid and semi‐arid environments. The estimation and prediction of spatial soil salinity may be considered as a stochastic process, observed at irregular locations in space. Environmental variables usually show spatial dependence among observations which is an important drawback to traditional statistical methods. Geostatistical techniques that analyse and describe the spatial dependence and quantify the scale and intensity of the spatial variation, provides spatial information for local estimation of soil salinity. In this paper we propose a Gaussian Spatial Linear Mixed Model (GSLMM), which involves a non‐parametric term, accounting for a deterministic trend given by exogenous variables, and a parametric component defining the purely spatial random variation, possibly due to latent spatial processes. We focus here on the analysis of the relationship between soil electrical conductivity as a parameter related directly to soil salinity as well as sodium content (sodicity) to identify spatial variations in these parameters. This kind of methodology is demonstrated as a useful tool for environmental land management. Copyright © 2007 John Wiley & Sons, Ltd.     

Stress response and lipid composition in shrimp Litopenaeus vannamei fed diets enriched with squid or scallop viscera meal

We substituted 10% of fishmeal with scallop (Argopecten ventricosus) or squid (Dosidiscus gigas) by‐product meal in a feed for shrimp (Litopenaeus vannamei) growth assay that lasted 60 days and then shrimps were submitted to handling stress. Shrimp fed the control diet had smaller weight gain and lower recovery after stress. Prostaglandins in gills were affected by diet and stress, with significant differences in shrimp fed the control diet before and after the stress. Hydroperoxides were increased after stress, while isoprostanoids were higher in shrimp fed the control diet. The gene expression of Glutathione peroxidase and Catalase was increased on the stressed shrimp fed the scallop diet, with no differences among diets for Superoxide dismutase or Cyclooxygenase. Arachidonic acid in the phospholipid fraction of muscle was the only fatty acid affected by stress and diet. Cholesterol oxidation products were significantly higher in shrimp fed the scallop diet compared to shrimp fed the control diet before applying the stress. A PCA analysis grouped in one‐factor growth variables, as well as Superoxide dismutase, esterified astaxanthin and isoprostane, while another factor was determined by stress, prostaglandin and hydroperoxide levels in gills. The partial substitution of fishmeal with scallop of squid by‐product meals affected growth and stress differently, indicating shrimp that grew up more were not necessarily more tolerant to handling stress, which can have a strong repercussion in aquaculture, particularly during density control, recording of growth and harvest.     

Influence of paclobutrazol and substrate on daily evapotranspiration of potted geranium

The application of plant growth regulators and the use of different culture media are common practices in potted ornamental crops. We report the results of a study to evaluate the effect of two substrates (peat moss or coconut fiber) and spraying 30 ppm paclobutrazol (PAC) on water resource management of zonal geranium (Pelargonium × hortorum L.H. Bailey) seedlings growing in a greenhouse during spring–summer. Growth, water consumption, leaching, evapotranspiration rate (ET), water use efficiency (WUE) and leaf water potential were determined. Plant growth was greater in peat moss (peat) than in coconut fiber (coconut), while the use of PAC decreased plant growth in both substrates, although more markedly in peat. Coconut produced a higher leaching fraction than peat in both PAC-treated plants and non-treated plants, although PAC application increased the leaching fraction in both substrates. Water consumption was lower in coconut than in peat and decreased in response to PAC. Peat was more effective at improving the water-use efficiency and increased the electrical conductivity in the leachate, while PAC decreased the latter in this same substrate. Daily ET showed a maximum of 200 mL/pot in peat and a minimum of 60 mL/pot in the coconut-PAC treatment. During the experimental period, accumulated ET values were higher in peat than in coconut, while the application of PAC reduced the value in peat but increased it in coconut. The evolution of ET per hour during the day showed that the highest rate (23 mL/h) was registered at midday in peat, while about 90% of ET occurred between 10 and 18 h in all treatments. When a cycle of water stress and recovery was applied, the peat induced the highest ET values of all treatments during the first days of drought, but the lowest ET after a week of drought. PAC modified this response, favoring a more balanced behavior of ET, although after the second cycle of drought this effect disappeared. Based on these results, the best strategy in normal irrigation conditions would be to use coconut fiber and not apply PAC, because this provides plants of excellent commercial quality with low water consumption. In plants at moderate water deficit PAC played a more important role than the substrate, improving the water status of plants with both substrates used. However, in the face of severe water stress the effect of PAC disappeared and peat acted as a better substrate than coconut fiber.     

The rehabilitation of degraded soils in eastern bolivia by subsoiling and the incorporation of cover crops

A high proportion of the soils in the central zone of Santa Cruz, eastern Bolivia, are chemically and physically degraded, with low organic matter and N contents, compacted subsoil layers and a propensity to crusting, hardsetting and wind erosion. The aim of the experiment discussed in this paper was to identify suitable cover crops to be used in combination with subsoiling for the rehabilitation of degraded soils and the improvement of crop yields in eastern Bolivia. Fertilizers were not used because of their high cost. An experiment with a split complete block design, with subsoiling and no-subsoiling as the main treatments, 14 cover crops and a continuously cultivated soybean/wheat control as the subtreatments, and four replications, was established on a degraded site comprising a mosaic of two compacted siliceous isohyperthermic soils (a coarse loamy Typic Ustropept and a fine loamy Typic Haplustalf). After a two-year fallow period, the cover crops were incorporated and test crops were sown for five seasons to evaluate the effects of the treatments on subsequent crop yields. Soil samples were taken to measure changes in chemical fertility. The only significant cover crop effect on soil nutrients was an increase in exchangeable K from 0.47 to 0.56 cmol_c kg⁻¹ by Lablab; subsoiling had no effect on chemical fertility. For all treatments there was an average 24 per cent increase in soil organic matter from 13.1 g kg⁻¹ at 3 months after cover crop incorporation to 16.3 g kg⁻¹ at 19 months after incorporation. No significant differences in total N were found during this period. Test crop yields were not influenced by subsoiling, but were significantly increased by some of the cover crops as compared to the soybean/wheat control during the first three seasons only. Evidence from foliar analysis suggests that the effects of the cover crops on soybean yields were not nutritional and so presumably were physical in nature, whereas the benefits on wheat yields were possibly related to increased N availability. Panicum maximum var. Centenario and P. maximum var. Tobiatá gave the highest total yield increases over the first three cropping seasons (101 and 85 per cent, respectively), but these yield increases would not compensate the farmer for the loss of four crop harvests whilst the land was in fallow. These results highlight the difficulties of rehabilitating soil fertility and increasing crop yields through the use of subsoiling and cover crop fallows on compacted, low organic matter soils in eastern Bolivia.     

What controls fish functional diversity patterns in Colombian Andean streams?

Understanding the mechanisms that structure communities have been a major challenge in ecological theory. Functional trait-based approaches are increasingly used for studying the processes underlying community assembly. However, species-poor systems of Andean streams these processes are largely unknown. Tropical Andean streams are highly variable in space-time and characterised by the harsh or stable environment, resulting in a high taxonomic and ecological diversity. In this study, we tested the influence of environmental variables on the fish functional structure. We proposed that in ecosystems with harsh environments, environmental filtering will structure fish assemblages, and in ecosystems with a more stable environment, limiting similarity will prevail, increasing functional diversity. We analysed fish assemblages using functional diversity indices, fourth-corner RLQ analyses and multiple regression models. Environmental variables such as altitude, stream width, sub-Andean vegetation and pH influenced functional diversity. For instance, high values of functional redundancy were registered at high elevation streams, reflecting fish assemblages composed of highly dominant species with similar functional traits such as species of the genus Astroblepus. However, we found differences between Astroblepus species in the number and distance of gill rakers which might indicate resource partitioning and coexistence. On the other hand, our results show both stochastic dynamics and environmental filtering are structuring fish assemblages in low and high elevation Andean streams respectively.