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用40条10碱基随机引物,对普通小麦“中国春”、“中国春”-杀配子染色体2C二体附加系、柱穗山羊草进行RAPD分析,筛选到1个杀配子染色体2C特异引物OPF03。从“中国春”-杀配子染色体2C二体附加系中克隆了该DNA片段。测序结果表明,该片段长1 496 bp,与大麦1个cDNA的同源性为92%。根据OPF031496的序列设计了1对SCAR引物2C-F586、2C-R586,对普通小麦“中国春”、“中国春”-杀配子染色体2C二体附加系、柱穗山羊草、二倍体长穗偃麦草、“中国春”-长穗偃麦草7E二体附加系等材料进行了SCAR分析,在“中国春”-杀配子染色体2C二体附加系、柱穗山羊草中扩增出了586 bp的片段,而在普通小麦“中国春”、二倍体长穗偃麦草、“中国春”-长穗偃麦草7E二体附加系中没有该产物,初步证明此标记可以用于杀配子染色体2C的检测。进一步用该对SCAR引物对“中国春”-杀配子染色体2C二体附加系与“中国春”-长穗偃麦草7E二体附加系的杂种F1代、F2代进行了分析,结果在全部F1代以及部分F2代材料扩增出了目的片段,进一步证明了此SCAR标记可以用来检测小麦背景下的杀配子染色体2C,为小麦背景中杀配子染色体2C的快速跟踪检测提供了新途径。 相似文献
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Hayato AMAIKE Motoki SASAKI Nao TSUZUKI Mitsunori KAYANO Motoharu OISHI Kazutaka YAMADA Hideki ENDO Tomoko ANEZAKI Naoya MATSUMOTO Rumiko NAKASHITA Misako KUROE Hajime TARU Gen BANDO Yuko IKETANI Ryohei NAKAMURA Nobutaka SATO Daisuke FUKUI Nobuo KITAMURA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(8):1284
In several primates and carnivores, pronation/supination angles of the forearm skeleton were examined, and it is thought that a larger angle is useful to acquire dexterous behaviors in feeding and/or life style, including climbing. In this study, the pronation/supination angles in Asiatic black, brown and polar bears were nondestructively examined. These specimens were classified as adult or non-adult. Three or four carcasses of each group of Asiatic black and brown bears were used for CT analysis, whereas only one adult polar bear was used. The forearms were positioned within the gantry of a CT scanner in both maximally supinated and pronated states. Extracted cross-sectional CT images of two positions were superimposed by overlapping the outlines of each ulna. The centroids of the radii were detected, and then the centroid of each radius and the midpoint of a line which connects between both ends of the surface of each radius facing the ulna, were connected by lines to measure the angle of rotation as an index of pronation/supination. In adult brown and polar bears, the angles were smaller as compared with the other groups (Asiatic black and non-adult brown bears). Asiatic black and non-adult brown bears can climb trees, whereas adult brown bears and polar bears cannot. This suggests that the pronation/supination angle is related to arboreal activity in Ursidae. 相似文献
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Hajime KUMAGAI Shuichiro KUMAGAE Katsunosuke MITANI Tsuyoshi ENDO 《Animal Science Journal》2004,75(3):219-224
To study the effects of supplementary probiotics on dry matter intake (DMI), daily gain (DG), digestibility, ruminal pH, and fecal microbial populations and metabolites in ruminants, two reversal trials were conducted by using four Suffolk ewes fitted with rumen cannula. The ewes were fed with oat hay and with concentrate and oat hay in the ratio of 60 : 40 in experiments 1 and 2, respectively. The ewes in the treatment groups were supplemented with 10 g/day/head probiotics for 49 days. Fresh fecal samples were collected to measure microbial populations and metabolites. On days 43–47 total feces was collected to measure digestibility, and on the days 48 and 49 ruminal pH was measured. No significant difference of DMI, DG, dry matter digestibility, and ruminal pH was observed between the control and treatment groups. The probiotics treatment tended to increase crude fiber (P = 0.11) and organic cell wall digestibility (P = 0.18). In the final week, probiotics treatment significantly increased the fecal population of Bacilli (P < 0.05) and mold (P < 0.01) in experiment 1 and 2, respectively. No significant difference of fecal VFA and ammonia concentrations between the control and treatment groups was observed. The supplementary probiotics changed population of some microbial strains in the feces and possibly the large intestine of ewes. 相似文献
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Sho FUKUI Yoshifumi ENDO Kazuko HIRAYAMA Hiroyuki TANIYAMA Tsuyoshi KADOSAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(6):747-751
Simultaneous removal of bilateral thyroid tumors was performed while preserving the
parathyroid gland in six dogs. At least one external parathyroid gland was identified in
all dogs. In five cases, the external parathyroid gland and its blood supply were
preserved intact. In one dog, the vessels supplying the external parathyroid gland had
been invaded by the tumor, and the gland was thus removed and reimplanted into the
sternohyoid muscle. That dog required postoperative treatment with oral calcium gluconate
and vitamin D3. Local tumor recurrence was not observed in any of the cases.
The mean survival time was 920 days. We found that the external parathyroid gland could be
identified and preserved in most dogs undergoing total thyroidectomy. 相似文献
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Reiko ABE Kyoko FUJIMOTO Yuko ENDO Katsuaki SUGIURA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(12):1826
The sales amount of antimicrobials intended for use in dairy cattle, beef cattle and broilers from 2008 to 2019 was evaluated for each antimicrobial class and administration route using dosage-based indicators. Our results revealed that the antimicrobial sales amount sold for use in dairy cattle in 2019 in terms of total weight of active ingredient, the number of defined daily doses (DDDs) (theoretical amount of biomass subjected to antimicrobial treatment in a year) and the number of treatment days (TDs) (theoretical number of days of treatment that an animal is subjected to in a year) calculated using Japanese DDD values (DDDjp values) was 36,751 kg, 8,261,848,000 kg·days and 15.5 days, respectively. Likewise, the antimicrobial sales amount sold for use in beef cattle and broilers in 2019 in terms of these metrics was 33,403 kg, 3,928,248,000 kg·days and 3.61 days, and 69,773 kg, 2,947,848,000 kg·days and 10.66 days, respectively. There was a considerable difference between the number of DDDs calculated using DDDjp values and that calculated using European DDD values (DDDvet values) for antimicrobial products sold for use in dairy and beef cattle. Our results also revealed that the sales amount of some antimicrobials, such as cephalosporins and quinolones represented larger proportions when calculated using dosage-based indicators than when calculated using the weight of active ingredient. Considering that Japanese veterinarians and farmers are more likely to conform to the Japanese dosage recommendations rather than the European ones, these results indicate the need for using dosage-based metrics, in particular metrics based on Japanese dosages rather than European dosages. 相似文献
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Yuki ANDO Dai ISHIYAMA Hideshige SHIMIZU Natsumi ENDO Tomomi TANAKA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(10):1373
Changes in the C-reactive protein (CRP) and 13,14-dihydro-15-keto-prostaglandin F2α (PGFM) concentrations of uterine lavage fluid were examined in cows given an intrauterine povidone-iodine (PI) infusion. The mean polymorphonuclear leukocyte (PMN) ratios (the ratio of PMN to total cells) and CRP concentration of uterine lavage fluid on the day after the treatment were significantly (P<0.05) greater in the PI infusion group (PMN: 53.0 ± 32.7%, CRP: 50.2 ± 32.3 ng/mL) than in the non-treatment control group (PMN: 7.9 ± 21.9%, CRP: 17.2 ± 5.9 ng/mL), whereas there was no significant difference in the mean PGFM concentration between the two groups. The present findings suggest that the uterine CRP level is a useful biomarker of local uterine inflammation in cows. 相似文献
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Mari OKAMOTO Keisuke OGUMA Nanako YAMASHITA-KAWANISHI Toshihiro ICHIJO Shinichi HATAMA Maiko ENDO Maya ISHIKAWA Takeshi HAGA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2020,82(11):1607
Bovine foamy virus (BFV) is distributed through worldwide cattle herds. Although the biological features of BFV are not well understood, appearance of clinical manifestation by superinfection with other microorganisms is inferred. In Japan, reports of genomic characterizations and epidemiology of this virus are limited. In this study, we performed whole genomic sequencing of BFV strains Ibaraki and No.43, which were isolated in this country. Additionally, we investigated BFV in geographically distant four daily farms in Japan, to estimate the distribution of BFV and its correlation to bovine leukemia virus (BLV). BFV was distributed throughout Japan; the average positive rate was 12.7%. The nucleotide sequence identities of the isolates were 99.6% when compared with BFV strain isolated in the USA. The phylogenetic tree using env gene sequence showed strains Ibaraki, No.43 and Kagoshima were sorted in the same cluster including the USA and Chinese strains, while Hokkaido strain was in the other cluster including European strains. Although no clear correlation between BFV and BLV could be found, BFV and BLV infections were likely to increase with ages. Our data on epidemiology and characteristics of BFV will provide important information to reveal biological features of BFV. 相似文献
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Yuria UMEMURA Ryosuke MIYAMOTO Rie HASHIMOTO Kyoko KINOSHITA Takuya OMOTEHARA Daichi NAGAHARA Tetsushi HIRANO Naoto KUBOTA Kiichi MINAMI Shogo YANAI Natsumi MASUDA Hideto YUASA Youhei MANTANI Eiko MATSUO Toshifumi YOKOYAMA Hiroshi KITAGAWA Nobuhiko HOSHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(12):1587-1598
Mammalian sexual fate is determined by the presence or absence of sex determining
region of the Y chromosome (Sry) in the “bipotential” gonads.
Recent studies have demonstrated that both male and female sexual development are induced
by distinct and active genetic pathways. Breeding the Y chromosome from Mus m.
domesticus poschiavinus (POS) strains into C57BL/6J (B6J) mice
(B6J-XYPOS) has been shown to induce sex reversal (75%: bilateral ovary, 25%:
true hermaphrodites). However, our B6N-XYPOS mice, which were generated by
backcrossing of B6J-XYPOS on an inbred B6N-XX, develop as males (36%: bilateral
testis with fertility as well as bilateral ovary (34%), and the remainder develop as true
hermaphrodites. Here, we investigated in detail the expressions of essential sex-related
genes and histological features in B6N-XYPOS mice from the fetal period to
adulthood. The onsets of both Sry and SRY-box 9 (Sox9) expressions as determined
spatiotemporally by whole-mount immunohistochemistry in the B6N-XYPOS gonads
occurred 2–3 tail somites later than those in B6N-XYB6 gonads, but earlier than
those in B6J-XYPOS, respectively. It is possible that such a small difference
in timing of the Sry expression underlies testicular development in our
B6N-XYPOS. Our study is the first to histologically show the expression and
ectopic localization of a female-related gene in the XYPOS testes and a
male-related gene in the XYPOS ovaries. The results from these and previous
experiments indicate that the interplay between genome variants, epigenetics and
developmental gene regulation is crucial for testis development. 相似文献
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Yoshiro ENDO Seiji HOBO Kenji KOROSUE Kenji OOTSUKA Akira KITAUCHI Risa KIKKAWA Yuichi HIDAKA Mitsuyoshi HAGIO Nao TSUZUKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(4):507-509
The present study evaluated the effects of single-dose filgrastim on hematology in 16
healthy horses after long-distance transportation. Horses were assigned to receive
filgrastim (0.23 µg/kg, SC, once; G-CSF group; n=8) or saline (0.9% NaCl)
solution (0.3 ml, SC, once; control group; n=8) ≤ 1 hr before
transportation. Horses were transported 2,530 km using commercial vans over the course of
approximately 44 hr. Clinical examinations and hematologic analyses were performed on all
horses before and after transportation. Because the post-transportation white blood cell
counts and bacillary neutrophil to segmented neutrophil ratio were significantly higher in
the G-CSF group, filgrastim may have promoted the mobilization of neutrophils from marrow.
Filgrastim deserves a further study for efficacy in preventing horse shipping fever. 相似文献