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Summary Traffic during alfalfa harvest operations can cause soil compaction and damage to newly growing stems. Root exploration for soil water and nutrients, forage growth dynamics, and final yield can all be affected. The objectives of this study were to determine the long-term effects of harvest traffic and soil compaction on water-use efficiency (WUE) of alfalfa grown in a Wasco sandy loam (coarse-loamy, mixed, nonacid, thermic Typic Torriorthents). Alfalfa was planted into tilled soil and managed with or without harvest traffic. Plants subjected to traffic during harvest had a significantly lower WUE two out of the three years studied compared to plants that were never subject to traffic. The second experiment examined whether planting alfalfa into compacted soil and managed with or without harvest traffic altered WUE. Soil compaction had no affect on alfalfa WUE. It was significantly lower when grown in compacted soil and subjected to harvest traffic. It is suggested that the decrease in WUE caused by harvest traffic may be explained by plants allocating carbohydrates to damaged shoots and crowns instead of to above ground forage production. The area of the field affected by harvest traffic, which damages newly growing stems, should be minimized to increase crop water use efficiency. 相似文献
44.
Natalie Turner Pevindu Abeysinghe Hassendrini Peiris Kanchan Vaswani Pawel Sadowski Nick Cameron Nathanael McGhee Jayden Logan Murray D Mitchell 《Journal of animal science》2022,100(2)
Cattle ticks pose a significant threat to the health and profitability of cattle herds globally. The investigation of factors leading to natural tick resistance in cattle is directed toward targeted breeding strategies that may combat cattle tick infestation on the genetic level. Exosomes (EXs), small extracellular vesicles (EVs) of 50 to 150 nm diameter, are released from all cell types into biofluids such as blood plasma and milk, have been successfully used in diagnostic and prognostic studies in humans, and can provide essential information regarding the overall health state of animals. Mass spectrometry (MS) is a highly sensitive proteomics application that can be used to identify proteins in a complex mixture and is particularly useful for biomarker development. In this proof of principle study, EXs were isolated from the blood plasma of cattle (Bos taurus) with high (HTR) and low tick resistance (LTR) (n = 3/group). Cattle were classified as HTR or LTR using a tick scoring system, and EXs isolated from the cattle blood plasma using an established protocol. EXs were subjected to MS analysis in data-dependent acquisition mode and protein search performed using Protein Pilot against the B. taurus proteome. A total of 490 unique proteins were identified across all samples. Of these, proteins present in all replicates from each group were selected for further analysis (HTR = 121; LTR = 130). Gene ontology analysis was performed using PANTHER GO online software tool. Proteins unique to HTR and LTR cattle were divided by protein class, of which 50% were associated with immunity/defense in the HTR group, whereas this protein class was not detected in EXs from LTR cattle. Similarly, unique proteins in HTR cattle were associated with B-cell activation, immunoglobins, immune response, and cellular iron ion homeostasis. In LTR cattle, unique exosomal proteins were associated with actin filament binding, purine nucleotide binding, plasma membrane protein complex, and carbohydrate derivative binding. This is the first study to demonstrate that MS analysis of EXs derived from the blood plasma of HTR and LTR cattle can be successfully applied to profile the systemic effects of tick burden. 相似文献
45.
Allysa L. Cole Natalie M. Kirk Leyi Wang Chien-Che Hung Jonathan P. Samuelson 《Journal of veterinary diagnostic investigation》2022,34(1):116
Two aborted Chester White pig fetuses were presented to a veterinary diagnostic laboratory in Illinois. Postmortem examination identified no gross abnormalities. Histologic evaluation revealed multifocal necrosis of chorionic epithelial cells, coalescing areas of mineralization in the placenta, and focal accumulations of viable and degenerate neutrophils in the lung. Intra- and extracellular acid-fast bacilli were identified in the lesions in both the placenta and lungs. Bacterial culture of stomach contents yielded heavy growth of Mycobacterium fortuitum, a rapidly growing nontuberculous mycobacterium (NTM), which was further confirmed through whole-genome sequencing. NTM are opportunistic pathogens commonly found in the soil and in contaminated water supplies. In animals, M. fortuitum is typically introduced through cutaneous wounds leading to infections limited to the skin, with systemic infection being uncommon. To our knowledge, abortion caused by M. fortuitum has not been reported previously. 相似文献
46.
K. L. Worrall C. G. Carter R. J. Wilkinson M. J. R. Porter 《Aquaculture International》2011,19(6):1075-1082
The efficacy of photoperiod manipulation to influence growth and developmental processes is well documented in a range of
temperate aquaculture species. However, the application of such techniques with tropical species requires further investigation.
This preliminary 20-day study investigated the influence of continuous photoperiod on growth of barramundi (Lates calcarifer). In addition, diel plasma melatonin profiles provided a physiological measure of how the endocrine system of barramundi
responded to continuous photoperiod. Juvenile barramundi (1.33 ± 0.02 g) were held in recirculation systems under 12-h light:
12-h dark (12L:12D) or 24-h light (24L:0D) with a light intensity of 1,000 lux throughout the water column. Fish from both
treatments grew to more than 14 times their original weight, with final weight (24L:0D = 21.59 ± 0.85 g; 12L:12D = 19.12 ± 0.55 g),
total length (24L:0D = 12.67 ± 0.14 cm; 12L:12D = 11.96 ± 0.13 cm) and specific growth rate (24L:0D = 9.60 ± 0.05% bw day−1; 12L:12D = 9.14 ± 0.06% bw day−1) being significantly higher for fish grown on 24L:0D compared with 12L:12D. There were no significant differences in feed
intake (24L:0D = 226.46 ± 6.27 g; 12L:12D = 219.02 ± 5.73 g) or feed conversion ratio (24L:0D = 0.71 ± 0.06; 12L:12D = 0.80 ± 0.07)
between light treatments. Barramundi held under 12L:12D exhibited diel melatonin secretion, which peaked mid-dark phase (171.83 ± 4.81 pg ml−1) followed by a gradual decrease in base levels at the onset of illumination (68.61 ± 8.77 pg ml−1). When juvenile barramundi were subjected to 24L:0D, the amplitude of peak melatonin secretion was significantly suppressed
during the subjective mid-dark phase (129.71 ± 2.36 pg ml−1). This preliminary study confirmed that barramundi respond to photoperiod manipulation in a similar manner to many temperate
fish species, thus demonstrating the future potential use of artificial lighting to improve growth in this species commercially. 相似文献
47.
Increasing use of spatial management tools in fisheries requires an understanding of fleet response, and in particular to where displaced fishing effort is likely to move. We develop a state‐dependent decision‐making model to address the spatial allocation of effort in an Australian tuna longline fishery. We assume that fishers have an economic objective in deciding where to fish, but that decisions in any period are also influenced by the remaining quota held at the time of the decision. Key features of the model include endogenous price dynamics, a moving stock and a competitive pool of different vessel types operating from different port locations. We utilize this model to illustrate fleet responses to marine reserves and limits on fishing effort. The results illustrate that the model framework provides advantages over statistically based models in that decisions made in response to the imposition of a reserve are not consistent with a proportional reallocation of effort. Rather, the stochastic dynamic model yielded an overall profit level of ~4% higher relative to scenarios with no reserve. Incorporating the opportunity cost of a quota into the model resulted in an optimal utilization of effort, in which effort was concentrated in time periods and locations yielding maximized profit. Under a low level of effort relative to the season length, the model indicated an overall profit level 43% greater than the highest obtained when the same level of effort was applied solely within any given quarter of the season. 相似文献
48.
Protein and energy nutrition of brook trout (Salvelinus fontinalis) at optimal and elevated temperatures
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The digestible protein (DP) and digestible energy (DE) requirements for maintenance and growth of brook trout (Salvelinus fontinalis) were determined using a factorial model at either optimum (15 °C) or elevated temperature (19 °C). Several key parameters of the factorial model were measured using a series of inter‐related studies. The maintenance requirements for DP and DE were 0.10 gDP kg?0.69 day?1 (15 °C) and 0.31 gDP kg?0.78 day?1 (19 °C), and 34.86 kJDE kg?0.84 day?1 (15 °C) and 46.14 kJDE kg?0.86 day?1 (19 °C). The total requirements for DP were 0.10 gDP kg?0.69 day?1 + 2.14PG (protein gain) (15 °C) and 0.31 gDP kg?0.78 day?1 + 1.98PG (19 °C). The total requirements for DE were 36.86 kJDE kg?0.84 day?1 + 1.58EG (energy gain) (15 °C) and 46.14 kJDE kg?0.86 day?1 + 1.64EG (19 °C). The partial efficiencies for growth were 0.47 (15 °C) and 0.51 (19 °C) for protein, and 0.63 (15 °C) and 0.61 (19 °C) for energy. Nutrient gain was lower at the elevated temperature; however, feed formulation for brook trout should be adjusted to match changes in nutrient requirements at different culture temperatures. The protein and energy requirements model will be useful for developing commercial feeds and feeding charts for brook trout. 相似文献
49.
The question of what determines plant community composition is fundamental to the study of plant community ecology. We examined
the relative roles of historical land use, landscape context, and the biophysical environment as determinants of plant community
composition in regenerating citrus groves in north-central Florida. Results were interpreted in light of plant functional
traits. Herbaceous and woody plants responded differently to broad-scale variables; herbs correlated most strongly with surrounding
land cover at a scale of 8 km, while the only significant determinant of woody species distributions was local land use history.
There were significant correlations between herbaceous species and spatial context, habitat isolation, environmental variables,
and historical variables. Partial Mantel tests indicated that each variable provided a unique contribution in explaining some
of the variation in the herbaceous dataset. The correlation between woody plants and local historical variables remained significant
even with other effects corrected for. In the herbaceous community, species composition was linked to functional traits much
as expected from classical theory. While spatial influences in our study system are important for both woody and herbaceous
plants, the primary determinant of plant community composition in regenerating citrus groves is historical land use. Our results
suggest that the fine-scale mechanisms of local competition, tolerance and facilitation invoked by many classical studies
may ultimately be less important than land use history in understanding current plant community composition in regenerating
agricultural areas. 相似文献
50.