Antagonistic activity of <Emphasis Type="Italic">Trichoderma</Emphasis> spp. against <Emphasis Type="Italic">Scytalidium lignicola</Emphasis> CMM 1098 and antioxidant enzymatic activity in cassava

Trichoderma spp. are used as antagonists against different pathogens. Despite many possibilities of using Trichoderma as an antagonist, there are gaps in the knowledge of the interaction between Trichoderma, cassava and Scytalidium lignicola. This fungus causes cassava black root rot and is an inhabitant of the soil, so it is difficult to control. Antagonists may contribute to the possible induction of resistance of plants because, when exposed to such pathosystems, plants respond by producing antioxidative enzymes. The test for potential inhibition of growth of S. lignicola CMM 1098 in vitro was performed in potato-dextrose-agar with two Trichoderma strains T. harzianum URM3086 and T. aureoviride URM 5158. We evaluated the effect of the two selected Trichoderma to reduce the severity of cassava black root rot and shoots. Subsequently, the production of enzymes (ascorbate peroxidase, catalase, peroxidase and polyphenol oxidase) was evaluated in cassava plants. All two Trichoderma strains show an inhibition of the growth of S. lignicola CMM 1098. The most efficient was T. harzianum URM 3086, with 80.78% of mycelial growth inhibition. T. aureoviride URM 5158 was considered the best chitinase producer. All treatments were effective in reducing severity, especially treatments using Trichoderma. Cassava plants treated with T. aureoviride URM 5158 had the highest enzyme activity, especially peroxidase and ascorbate peroxidase. Trichoderma harzianum URM3086 and Trichoderma aureoviride URM 5158 were effective in reducing the severity of cassava black root rot caused by S. lignicola CMM 1098.     

Maintaining post‐grazing sward height of Panicum maximum (cv. Mombaça) at 50 cm led to higher animal performance compared with post‐grazing height of 30 cm

In swards of tall, tufted, tropical grasses like Mombaça guinea grass (Panicum maximum (Jacq.)), post‐grazing heights promote changes in sward structure, which influence animal performance. This study evaluated changes in sward structure in response to grazing management at two post‐grazing heights (30 and 50 cm), associated with 90 cm pre‐grazing height. Each treatment was allocated to experimental unit (1.5 ha) in three replicated blocks. Pastures were evaluated pre‐ and post‐grazing to estimate herbage mass, percentages of leaf (LP), stem (SP) and dead material (DP), and nutritive value (VN). Stocking rate was adjusted twice a week “using the put‐and‐take approach,” and animals were weighed every 28 days. Forage accumulation rate was greater for pasture managed at 30 cm (64.8 vs. 55.1 kg ha^?1 day^?1) than 50 cm. A greater number of days were required after grazing 30‐cm residual pasture to achieve the pre‐grazing target height, resulting in 25% decrease in number of grazing cycles compared with pastures managed at 50 cm. Regardless of post‐grazing height targets, SP and DP decreased, whereas LP and NV increased from soil level to top of canopy. Stocking rate was greater in pastures managed to 30 cm than in those managed to 50 cm residual height (4.7 vs. 3.4 AU ha^?1). However, average daily gain was greater for 50 cm than for 30 cm post‐grazing height (795 vs. 590 g steer^?1 day^?1), resulting in a greater animal production per area (917 vs. 794 kg/ha of live weight). Thus, Mombaça guinea grass subject to intermittent grazing should be managed at 50 cm residual height.     

Lethal and sublethal responses of <Emphasis Type="Italic">Sitophilus zeamais</Emphasis> populations to essential oils

The present study aimed to assess the lethal and sublethal responses of five populations of S. zeamais to the essential oils of Ocimum basilicum and Piper hispidinervum, through toxicity, locomotor behavior (flight and walking activities) and physiological (respiration rate and body mass) evaluations. The populations of S. zeamais were obtained from Machado—MG, Paracatu—MG, Piracicaba—SP, Recife—PE and Tunápolis—SC. To estimate the LC₅₀ of each oil for each population, fumigation tests were performed. The population from Recife exhibited the lowest instantaneous rate of increase (ri) as well the lowest consumption of grain mass. However, Piracicaba was considered the susceptibility pattern, presenting the lowest LC₅₀ for both essential oils, no statistical difference was observed among populations or treatments in the flight activity bioassays. The essential oils effect on walking activity and respiration rates varied among populations. The population from Recife presented the highest walked distance and walk speed, with the lowest resting time and number of stops, as well as the lowest respiration rate and body mass. The present study highlights the importance of the study on population responses, as the five populations of S. zeamais exhibited different patterns of toxic and behavioral effects when subjected to the essential oils of P. hispidinervum and O. basilicum.