Stability of abdominal fat and meat of broilers: Effect of duration of feeding antioxidants 1

1. The α‐tocopherol (AT) content of abdominal fat and fat stability increased as a function of the duration of α‐tocopheryl acetate (ATA) feeding in broilers fed on diets containing either 40 g acidulated soyabean‐oil soapstock (ASS) or tallow/kg. A linear relationship was observed between AT content of the adipose tissue and its stability with both types of fat supplements.

2. The stabilities of carcass fat and meat of 9‐week‐old broilers fed on diets containing ASS with 300 mg ethoxyquin (EQ)/kg or diets containing tallow with 20 mg ATA/kg from weeks 5 to 9 were comparable with those obtained by feeding these antioxidants and fats throughout the growth period.

3. ATA added to a diet containing ASS, and EQ, added to a diet containing tallow did not improve meat stability despite such an effect on adipose tissue when fed for 9 weeks. The results emphasise the importance of using the dietary antioxidant appropriate to the fat supplement to improve carcass stability.     

Control of the cowpea aphid, Aphis craccivora Koch (Homoptera: Aphididae), in cowpea, Vigna unguiculata (L.) Walp.

Cowpea, Vigna unguiculata (L.) Walpers, is a major dietary staple in tropical Africa where it is most often cultivated. The production is, however, greatly hampered by severe infestation and damage by insect pests including the cowpea aphid, Aphis craccivora Koch. The damage and bionomics of the aphid are briefly introduced. Chemical, cultural, physical and biological methods, and use of plant resistance for A. craccivora control in cowpea are reviewed. Finally, prospects for integrated pest management are discussed     

Viruses in the northern potato-producing regions of Saudi Arabia

Agar double diffusion tests and, later, ELISAs were used to detect viruses associated with potato in 242 samples collected in 16 trips to Tabuk and Hail, northern regions of Saudi Arabia, in four consecutive growing seasons (autumn 1989, spring and autumn 1990 and spring 1991). Eleven different viruses were detected in Tabuk and 12 in Hail. The viruses detected in Tabuk were alfalfa mosaic (AMV), cucumber mosaic (CMV), tobacco mosaic (TMV), potato leaf roll (PLRV), tomato spotted wilt (TSWV), tobacco ringspot (TRSV) and potato A, M, S, X and Y. The same viruses, plus potato yellow dwarf (PYDV), were detected in Hail. AMV was most frequently and CMV least frequently detected in Tabuk, whereas in Hail the most and least common were PVA and PLRV respectively.     

Eutypa canker and dieback of almonds

Eutypa canker and dieback of almonds was first recorded in commercial plantations in Greece in mid-1980 and confirmed as due to the ascomycetous fungus Eutypa lata. The disease manifests itself by the formation of cankers at the point of the junction of the branches and the trunk and is associated with pruning wounds made by the growers in order to confront the'non-infectious bud failure'disorder or to remove unwanted limbs close to the crotch. Pathogenicity tests made on young trees of cv. Texas with different isolates originated from grapevine, almond, apricot and pear showed significant differences in virulence. Pathogenicity tests carried out on 20 different almond cultivars gave a significant indication of the existence of tolerant and susceptible cultivars.     

The effect of sowing date, stale seedbed, row width and mechanical weed control on weeds and yields of organic winter wheat

Three field experiments were carried out in organically grown winter wheat in Denmark. The treatments were sowing time (normal or late sowing) and false seedbed, row width (12 and 24 cm) and weed control method [untreated; mechanical weed control (weed harrowing at 12 cm supplemented with inter‐row hoeing at 24 cm); and herbicide weed control]. Weed biomass in midsummer was greatest on plots sown at the normal sowing time (compared with delayed sowing) and was reduced by mechanical or chemical weed control (compared with untreated plots). Row width alone had no influence on weed biomass, but in the experiment with high weed pressure, the more intensive mechanical weed control used at a row width of 24 cm reduced weed biomass. Normal sowing time tended to give higher yields, but this was only statistically significant in one of the three experiments. Wide rows gave a yield decrease in the experiment with low weed pressure. The effect of weed control on yield was dependent on the weed pressure. At low weed pressure, mechanical weed control caused a yield decrease compared with untreated or herbicide treated. At intermediate weed levels there were no differences, whereas at high weed pressure, mechanical weed control and herbicide treatment caused a yield increase compared with untreated. False seedbeds were shown to contribute to a decrease in the soil seed reserve.     

Metabolic Profiling and In Vitro Assessment of the Biological Activities of the Ethyl Acetate Extract of Penicillium chrysogenum “Endozoic of Cliona sp. Marine Sponge” from the Red Sea (Egypt)

Marine sponge-derived endozoic fungi have been gaining increasing importance as promising sources of numerous and unique bioactive compounds. This study investigates the phytochemical profile and biological activities of the ethyl acetate extract of Penicillium chrysogenum derived from Cliona sp. sponge. Thirty-six compounds were tentatively identified from P. chrysogenum ethyl acetate extract along with the kojic acid (KA) isolation. The UPLC-ESI-MS/MS positive ionization mode was used to analyze and identify the extract constituents while 1D and 2D NMR spectroscopy were used for kojic acid (KA) structure confirmation. The antimicrobial, antioxidant, and cytotoxic activities were assessed in vitro. Both the extract and kojic acid showed potent antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa with MIC 250 ± 0.82 µg/mL. Interestingly, the extract showed strong antifungal activity against Candida albicans and Cryptococcus neoformans with MIC 93.75 ± 0.55 and 19.53 ± 0.48 µg/mL, respectively. Furthermore, KA showed the same potency against Fusarium oxysporum and Cryptococcus neoformans with MIC 39.06 ± 0.85 and 39.06 ± 0.98 µg/mL, respectively. Ultimately, KA showed strong antioxidant activity with IC₅₀ 33.7 ± 0.8 µg/mL. Moreover, the extract and KA showed strong cytotoxic activity against colon carcinoma (with IC₅₀ 22.6 ± 0.8 and 23.4 ± 1.4 µg/mL, respectively) and human larynx carcinoma (with equal IC₅₀ 30.8 ± 1.3 and ± 2.1 µg/mL, respectively), respectively. The current study represents the first insights into the phytochemical profile and biological properties of P. chrysoenum ethyl acetate extract, which could be a promising source of valuable secondary metabolites with potent biological potentials.     

The Impact of Natural Deep Eutectic Solvents and Extraction Method on the Co-Extraction of Trace Metals from Fucus vesiculosus

In recent years, natural deep eutectic solvents (NADES) have been widely investigated for the extraction of food and medicinal plants as well as seaweeds. However, the ability of NADES for trace elements co-extraction from natural sources is not well investigated. The aim of this study was to investigate the ability of common NADES for trace elements co-extraction from Fucus vesiculosus. All of the tested NADES did not recover As and Co (concentration <LOQ). Moreover, all of the tested NADES provided a low recovery (<9%) of Ba, Ca, Fe, Mg, Mn, Sr, and Zn. The method of extraction had not shown a statistically significant effect on the co-extraction of all elements (excluding Ba and Ca). In contrast, the water content in NADES was significantly affected on the recovery of Ba, Ca, Mg, Mn, Sr, and Zn. The recovery of Al and Cr was relatively high and considerably varied (from 1.5 to 59.9%). NADES comprising lactic acid:glucose:H₂O (5:3:1) provided the lowest contents of all elements, and the highest extracted amounts were obtained employing water contents of 60–80%. The calculated daily intake of all the elements contained in NADES extracts were less than the daily dose risk estimators. The hazard quotients, hazard indexes, and carcinogenic risk calculated for all trace elements and their combination were considerably less than 1. This evidences no health risk, and carcinogenic risk after topical application of all studied NADES. For the first time, the results of the current study demonstrated that NADES extracts of F. vesiculosus contain a lower amount of trace metals and are safer than the extracts obtained with water and 70% acetone. This indicates a significant advantage for NADES compared with the other solvents.     

The effect of propionic acid on the storage losses of hay

Storage losses in moist hay treated with propionic acid were compared with those occurring in untreated moist hay in two experiments and with field-cured hay in three experiments. Dry matter losses in treated hays ranged from 41 to 8.6% and from 1.7 to 12.6% in untreated hays. Digestible organic matter losses in both treated and untreated hays were generally greater than dry matter losses. Only in one experiment did propionic acid application significantly reduce nutrient losses in moist hays. Nutrient losses, and water-soluble carbohydrate losses in particular, were shown to be correlated with maximum and cumulative bale temperatures during storage in two of the experiments. The rise in hale temperatures during storage was reduced in all three experiments, to a greater or lesser extent, by application of the additive. Loss of propionic acid from the hay during and after application was very large. In the three experiments 86.2, 85.3 and 85.6% of the acid applied was lost by the end of the storage period. Acid distribution studies indicated that variation in acid concentration within bales was as great as between bales. It was concluded that more research is needed into applicator design and position on the baler and into alternative application methods if the benefits of propionic acid as a moist hay preservative are to be fully exploited.