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101.
Hiroaki Maeda Takuya Yamaguchi Motoyasu Omoteno Takeshi Takarada Kenji Fujita Kazumasa Murata Yukihide Iyama Yoichiro Kojima Makiko Morikawa Hidenobu Ozaki Naoyuki Mukaino Yoshinori Kidani Takeshi Ebitani 《Breeding Science》2014,64(2):134-141
Rice (Oryza sativa L.) can produce black grains as well as white. In black rice, the pericarp of the grain accumulates anthocyanin, which has antioxidant activity and is beneficial to human health. We developed a black rice introgression line in the genetic background of Oryza sativa L. ‘Koshihikari’, which is a leading variety in Japan. We used Oryza sativa L. ‘Hong Xie Nuo’ as the donor parent and backcrossed with ‘Koshihikari’ four times, resulting in a near isogenic line (NIL) for black grains. A whole genome survey of the introgression line using DNA markers suggested that three regions, on chromosomes 1, 3 and 4 are associated with black pigmentation. The locus on chromosome 3 has not been identified previously. A mapping analysis with 546 F2 plants derived from a cross between the black rice NIL and ‘Koshihikari’ was evaluated. The results indicated that all three loci are essential for black pigmentation. We named these loci Kala1, Kala3 and Kala4. The black rice NIL was evaluated for eating quality and general agronomic traits. The eating quality was greatly superior to that of ‘Okunomurasaki’, an existing black rice variety. The isogenicity of the black rice NIL to ‘Koshihikari’ was very high. 相似文献
102.
103.
Many bony fish type I collagens have a characteristic third chain designated as α3(I). However, much less is known about the primary structure and distinction of the proα(I) chains. Their cDNAs were cloned by RT-PCR from the muscle tissue of Japanese eel, Anguilla japonica. Three cDNAs coding for the triple-helical domain of fibrillar collagen were identified as proα1(I), proα2(I) and proα3(I) chains by sequencing selected tryptic peptides isolated from eel type I collagen subunit chains, α1(I), α2(I) and α3(I). Eel proα3(I) had high amino acid sequence identity (81 %) to its proα1(I). The distribution of seven Cys residues in the C-propeptide of proα3(I) was identical to that of proα1(I). There was a third Cys residue at the 1,268th position from the N-terminus in proα1(I), though a supposed Cys residue at the 1,264th position in proα3(I) was replaced by a Ser residue. Similar replacement has been observed in the proα3(I) chains of trout and zebrafish. These combined results suggest that replacement of the Cys residue allows for the identification of fish collagen proα(I) previously not identified as proα3(I). 相似文献
104.
Amasaki H Arai R Ogawa M Takemura N Yamagami T Nagasao J Mutoh K Ichihara N Asari M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(4):541-543
In the present study, we examined specific markers for taste bud cells in the mouse and the postnatal development of volatile papilla taste bud cells in ddY mice. We examined the immunoreactivity of 4 types of carbonic anhydrase isoenzymes, CA I, CA II, CA III and CA VI, as specific markers for taste bud cells, and K8.13 cytokeratin antibody as a specific marker for the lingual epithelial cells. Of the carbonic anhydrase isoenzymes, only CA III immunoreactivity was clearly detected in the spindle shaped gustatory cells. CA VI immunoreactivity was detectable in suspentacular cells. CA I and CA II antibodies did not recognize any taste bud cell specifically. K8.13 cytokeratin immunoreactivity was detected in the lingual epithelial cells, but not in taste bud cells. At 7 days after birth, the suckling phase, very small taste buds developed from the anaplastic gustatory cells. At 14 days after birth, the taste buds showed larger size than those at 7 days after birth. At 21 days birth, after the weaning phase, taste bud structure approximated the mature structure. These results demonstrate the specificity of anti-CA III and anti-CA VI for gustatory cells and suspentacular cells, respectively. These markers should be useful for an analysis of taste bud development in mice. 相似文献
105.
Itoh N Muraoka N Kawamata J Aoki M Itagaki T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(2):161-163
Fecal samples obtained from 600 household cats (244 males and 356 females) kept in 3 prefectures of Tohoku district in Japan were examined for Giardia intestinalis antigen, using a commercial enzyme-linked immunosorbent assay (ELISA) kit. G. intestinalis antigen was detected in 40% of the fecal specimens. The factors such as the age, life style or environmental condition of cats could be significantly related to the positive rate of G. intestinalis antigen. In contrast, the investigative district, appearance of feces, sex, breed or origin produced no significant difference in the positive rate. The present results suggest that G. intestinalis infection is widely spread in household cats of Tohoku district. 相似文献
106.
Tominaga Y Miyagawa Y Toda N Takemura N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(8):971-975
We evaluated the diagnostic significance of the N-terminal pro-B-type natriuretic peptide (NT-proBNP) concentration in asymptomatic cats with cardiac enlargement. The plasma NT-proBNP concentration was measured in 21 clinically healthy control cats, and 67 asymptomatic cats with cardiac enlargement defined as end-diastolic interventricular septum thickness (IVSd) and/or diastolic left ventricular posterior wall thickness (LVPWd) >0.6 cm, vertebla heart scale (VHS) >7.8, and/or left atria/aorta ratio (LA/Ao) >1.5. The plasma NT-proBNP concentration in the asymptomatic cats with cardiac enlargement (median: 662.0, range: 24.0-2,449.0 pmol/l) was significantly higher than that in the controls (24.0, 24.0-95.0 pmol/l, P<0.001). The plasma NT-proBNP concentration was significantly correlated with the VHS, LA/Ao, IVSd and LVPWd (r=0.578, P<0.001; r=0.462, P<0.001; r=0.563, P<0.001; and r=0.764, P<0.001, respectively). Receiver operating characteristic analysis showed a cut-off value of 95.0 pmol/l for the detection of asymptomatic cats with cardiac enlargement, sensitivity and specificity of 88.1 and 100%, respectively, and an area under the curve of 0.971. These results suggest that the determination of the plasma NT-proBNP concentration can be a useful screening test for asymptomatic cats with cardiac enlargement. 相似文献
107.
108.
Yukari UETAKE Hitoshi NAKAMURA Masao ARAKAWA Ikuko OKABE Naoyuki MATSUMOTO 《Journal of General Plant Pathology》2001,67(3):175-181
A root box method with carrots was developed to estimate virulence of the violet root rot fungus, Helicobasidium mompa, to facilitate short-term screening of many isolates during a year. The root box consisted of two transparent acrylic plates
and a plastic bag of vermiculite in which two taproots of carrot were growing and inoculated with the fungus growing on fragments
of mulberry twigs. The boxes were kept in a greenhouse at 25°C, and the surface of carrots was observed weekly up to 14 weeks.
The virulence of each isolate was determined based on the number of weeks after inoculation required for the fungus to develop
infection cushions on the surface of carrots. Results were compared with those from the conventional inoculation method using
apple stocks. Two-year-old 456 apple stocks were planted with or without fungal inoculum in 30-cm diam. plastic pots containing
commercial soil and placed outdoors in April 1999. Symptoms on plant tops were observed weekly, and the first stocks were
killed 14 weeks after inoculation. At the end of trial 1 (6 months) and trial 2 (14 months), apple stocks were dug up to rate
disease index (DI) based on hyphal growth and infection cushion formation on the stem base. There was variability in disease
severity among replicates as well as isolate variability ; however, the results were similar in both trails. The level of
virulence estimated by both methods was almost parallel for a total of 23 isolates from five plant species, except for two
isolates from sweet potato that formed no obvious infection cushion on apple roots but on carrot were the most virulent.
Received 11 December 2000/ Accepted in revised form 23 March 2001 相似文献
109.
Tomioka Keisuke Sekiguchi Hiroyuki Nomiyama Koji Kawaguchi Akira Kawakami Akira Masunaka Akira Kobayashi Hidekazu Chiba Masahiro Nagata Kenji Ishikawa Naoyuki 《Journal of General Plant Pathology》2021,87(5):273-280
Journal of General Plant Pathology - A ripening disorder with early blighting of foliage including spike bleaching was observed on bread wheat in May 2017 and durum wheat in May 2018 in... 相似文献
110.
Naoyuki Matsunaga Shin-ichi Chisada Hideyuki Fujioka Kana Takashima Nozomu Okino Makoto Ito 《Fisheries Science》2011,77(4):583-590
Vibrios are highly motile, facultatively anaerobic bacteria that are ubiquitous in aquatic environments and part of the normal
intestinal microflora of healthy fish, but some species can cause vibriosis. The adherence of vibrios to host fish intestines
is a significant event not only for their survival and growth, but also in terms of pathogenicity. However, the molecular
mechanism underlying the adhesion of vibrios to the intestinal tract of fish is not fully understood. We report here the identification
of intestinal glycosphingolipid (GSL) receptors to which pathogenic vibrios attach in typical mariculture fish. Thin-layer
chromatography overlay assays using five species of 35S-labeled vibrios and intestinal glycosphingolipids of seven species of mariculture fish revealed that all of the fish tested
possessed GM3 (NeuAcα2-3Galβ1-4Glcβ1-1′Cer) and/or GM4 (NeuAcα2-3Galβ1-1′Cer) as major acidic intestinal GSLs and that all
of the vibrios tested specifically adhered to GM3 and/or GM4. Our results demonstrate that these GSLs were able to function
as a receptor for the various vibrios tested. Analysis of the relationship between sugar structure and receptor activity for
vibrios revealed that ‘NeuAcα2-3Galβ1-’ is required at the non-reducing end of glycosphingolipids for the bacteria to attach. 相似文献