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231.
Makoto Kimura Naoko Takahashi-Ando Takumi Nishiuchi Takeshi Tokai Makoto Fujimura Hiroshi Hamamoto Isamu Yamaguchi 《Pesticide biochemistry and physiology》2006,86(3):117-123
Fusarium head blight (FHB) is a devastating disease of important cereal crops resulting in significant yield loss and mycotoxin contamination. Persistent outbreaks of FHB in Europe and North America have led to various efforts to understand the mechanisms of resistance to this disease and mycotoxin biosynthesis. In this minireview, we summarize basic and applied studies conducted in our laboratories into reducing mycotoxin contamination in FHB. 相似文献
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233.
Mo CHEN Li-Yi CAI Naoko KANNO Takako KATO Jinxing LU Fan JIN Honghua WANG Masayo SEKITA Masashi HIGUCHI Saishu YOSHIDA Hideji YAKO Hiroki UEHARU Shun-Ichiro IZUMI Yukio KATO 《The Journal of reproduction and development》2013,59(5):457-462
Recently we demonstrated an ectopic expression of the human herpesvirus 1 thymidine
kinase (HHV1-TK) gene by functioning of an intrinsic endogenous promoter in the
transgenic rat (TG-rat), suggesting that HHV1 infection in humans induces expression
of the TK gene with the ectopic promoter in the testis and results in accumulation of
HHV1-TK protein, triggering male infertility similar to that in the TG-rat. Hence, in
this study, we started to investigate a relationship between infection of herpesvirus
and human male infertility. Semen was donated by Chinese male infertile patients (153
men, aged 21–49 years) with informed consent, followed by DNA preparation and
analysis by PCR and DNA sequencing. Semen volume, sperm number and density, and sperm
motility were examined. DNAs of HHV1, HHV4, HHV5 and HHV6 were confirmed by PCR,
electrophoresis and DNA sequencing. Finally, virus DNA was identified in 59 patients
(39%). The number of carriers was 39 (25%) for HHV1, 6 (4%) for HHV4, 33 (22%) for
HHV5 and 3 (2%) for HHV6, respectively. Moreover, double-infection was found in 22
out of 59 specimens (37%), most of which were double-infection of HHV1 and HHV5 (15
out of 22 carriers). Though slight severity was present in some of the carriers, the
relationship between virus infection and sperm impairment was not conclusive.
Accordingly, it is essential to examine whether the viral HHV1-TK gene is expressed
in the testis of the infertile human HHV carrier. 相似文献
234.
Takasao N Tsuji-Naito K Ishikura S Tamura A Akagawa M 《Journal of agricultural and food chemistry》2012,60(5):1193-1200
The breakdown of collagenous networks with aging results in hypoactive changes in the skin. Accordingly, reviving stagnant collagen synthesis can help protect dermal homeostasis against aging. We searched for type I collagen biosynthesis-inducing substances in various foods using human dermal fibroblasts and found that cinnamon extract facilitates collagen biosynthesis. Cinnamon extract potently up-regulated both mRNA and protein expression levels of type I collagen without cytotoxicity. We identified cinnamaldehyde as a major active component promoting the expression of collagen by HPLC and NMR analysis. Since insulin-like growth factor-I (IGF-I) is the most potent stimulator of collagen biosynthesis in fibroblasts, we examined the effect of cinnamaldehyde on IGF-I signaling. Treatment with cinnamaldehyde significantly increased the phosphorylation levels of the IGF-I receptor and its downstream signaling molecules such as insulin receptor substrate-1 and Erk1/2 in an IGF-I-independent manner. These results suggested that cinnamon extract is useful in antiaging treatment of skin. 相似文献
235.