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排序方式: 共有227条查询结果,搜索用时 31 毫秒
181.
Yui KOBAYASHI Tatsuro NAKAMURA Tomohiro YONEZAWA Koji KOBAYASHI Takahisa MURATA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(12):1977
Bacterial cystitis is one of the feline lower urinary tract diseases (FLUTDs). Polyunsaturated fatty acids, such as arachidonic acid (ARA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA), are oxidized into various lipid mediators that modulate inflammation. Since the profile of lipid metabolites excreted in urine is useful for assessing inflammatory body conditions, we analyzed 126 types of urinary lipid metabolites in cats with bacterial cystitis. Using LC-MS/MS, we found that the levels of 11 metabolites were higher in the urine of cystitis cats than in the urine of healthy cats. In detail, the urinary levels of ARA, EPA, and DHA and eight of their metabolites were increased in cystitis cats. Focusing on the lipid oxidation pathway, the urinary levels of four cyclooxygenase-, three lipoxygenase-, and one cytochrome P450-dependent oxidated metabolites were increased in bacterial cystitis. These urinary lipid profiles can provide some insight into the pathology and future diagnosis of bacterial cystitis. 相似文献
182.
Wataru YAMAMOTO Yasuhiro ITANO Tsunefumi KOBAYASHI Daishiro MIURA Yoshinori KASAHARA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(1):77-82
Human immunoglobulin G (IgG) concentrates are immune-modulating,
anti-inflammatory plasma-derived products. Clinical studies in recent years have suggested
that IgG attenuates neuropathic pain. In this study, effects of sulphonated IgG on the
development and maintenance of a mechanical allodynia-like response were examined in mice
with neuropathic pain induced by a partial sciatic nerve ligation (PSL). When sulphonated
IgG (400 or 1,000 mg/kg/day, i.p.) was administered for 5 days, from 1 day before surgery
to post-operative day (POD) 3, the development of a mechanical allodynia-like response was
attenuated. On the other hand, sulphonated IgG had little effect on the maintenance of a
mechanical allodynia-like response when administered for 5 days, from POD 11 to POD 15, at
which time a mechanical allodynia-like response had already been developed. To explore the
mechanism of sulphonated IgG, the mRNA expression of inflammatory cytokines was evaluated
in the injured sciatic nerve. Sulphonated IgG (1,000 mg/kg/day, i.p.) that was
administered for 3 days, from 1 day before surgery to POD 1, significantly attenuated the
up-regulation of tumor necrosis factor-α and monocyte chemotactic protein-1 mRNAs on POD
1. These results suggest that prophylactic treatment with sulphonated IgG attenuates the
development of mechanical allodynia-like response by inhibition of inflammatory cytokine
expression in mice with PSL. 相似文献
183.
Sota KOBAYASHI Toshiyuki TSUTSUI Takehisa YAMAMOTO Yoko HAYAMA Norihiko MUROGA Misako KONISHI Ken-ichiro KAMEYAMA Kenji MURAKAMI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(7):861-863
A cohort study was conducted to evaluate the risk of bovine leukemia virus (BLV)
transmission to uninfected cattle by adjacent infected cattle in 6 dairy farms. Animals
were initially tested in 2010–2011 using a commercial ELISA kit. Uninfected cattle were
repeatedly tested every 4 to 6 months until fall of 2012. The Cox proportional hazard
model with frailty showed that uninfected cattle neighboring to infected cattle (n=53) had
a significant higher risk of seroconversion than those without any infected neighbors
(n=81) (hazard ratio: 12.4, P=0.001), implying that neighboring infected
cattle were a significant risk factor for BLV transmission. This finding provides
scientific support for animal health authorities and farmers to segregate infected cattle
on farms to prevent spread of BLV. 相似文献
184.
Yumiko KAGAWA Yuko NAKANO Tetsuya KOBAYASHI Kazushi ASANO Satoshi TAKAGI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(12):1659-1661
Nineteen cases of histiocytic sarcomas in Pembroke Welsh Corgi were examined
histopathologically. Focal or multiple masses were detected in the lung or in regional
lymph nodes, or in both lung and nodes. All neoplastic lesions had common histological
features characterized by the proliferation of pleomorphic histiocytic cells combined with
various inflammatory cells. Most of the pleomorphic neoplastic cells were immunopositive
for human leukocyte antigen (HLA)-DR and Iba-1. The median survival time for all dogs was
133 days. In the present study, several prognostic factors, such as gender, age, single or
multiple lesions, lymph node involvement at the time of diagnosis, surgical resection
status and additional chemotherapy, were examined, although none of these factors
approached statistical significance. Histiocytic sarcoma must be considered in the
differential diagnosis of dogs with pulmonary masses, especially in the canine breed. 相似文献
185.
Takaku T Mikata K Matsui M Nishioka K Isobe N Kaneko H 《Journal of agricultural and food chemistry》2011,59(9):5001-5005
To estimate the metabolic profile of trans-permethrin in humans, a comparison of the in vitro metabolism of trans-permethrin in humans and rats was conducted using hepatic microsomes, and cytochrome P450 and UDP-glucuronyltransferase isoforms, which catalyze the metabolism of 3-phenoxybenzyl alcohol (PBalc) and 3-phenoxybenzoic acid (PBacid), respectively. In humans and rats, the major metabolic reaction of trans-permethrin in microsomal incubations was the cleavage of ester linkage to give PBalc, followed by oxidation to 4'-OH-PBalc, 4'-OH-PBacid, and PBacid. As to 4'-hydroxylation of PBalc, several CYPs were able to catalyze the reaction, and CYP2E1 was identified as a predominant isoform. PBacid and its conjugates (glucuronide and glycine) are major urinary metabolites of trans-permethrin in mammals. PBacid is also a metabolite of several pyrethroids, and has been used as a biomarker of human exposure to pyrethroids. Our study indicated that there was no difference in glucuronyltransferase activity of PBacid between humans and rats, and that only UGT1A9 can catalyze the glucuronidation of PBacid among human UGTs. Some UGT1A9 variants are known to have poor glucuronidation activity. From these results, it was assumed that deficiency or polymorphism of UGT1A9 might affect the profile of PBacid and its conjugates in urine collected from persons exposed to trans-permethrin or other pyrethroids. These results are helpful for understanding the metabolism of trans-permethrin in humans and determining methods for quantification of target analytes for assessment of human exposure to trans-permethrin and other pyrethroids that give PBacid and its conjugates as urinary metabolites. 相似文献
186.
Atsushi Ishii Keita Yamaji Yoshinobu Uemoto Nanae Sasago Eiji Kobayashi Naohiko Kobayashi Tamako Matsuhashi Shin Maruyama Hirokazu Matsumoto Shinji Sasazaki Hideyuki Mannen 《Animal Science Journal》2013,84(10):675-682
Fatty acid composition is one of the important traits in beef. The aim of this study was to identify candidate genomic regions for fatty acid composition by genome‐wide association study with 50 K single nucleotide polymorphism (SNP) array in Japanese Black cattle. A total of 461 individuals and 40 657 SNPs were used in this study. We applied genome‐wide rapid association using mixed model and regression (GRAMMAR) and genomic control approaches to estimate the associations between genotypes and fatty acid composition. In addition, two SNPs in fatty acid synthase (FASN) (T1952A) and stearoyl‐CoA desaturase (SCD) (V293A) genes were also genotyped. Association analysis revealed that 30 significant SNPs for several fatty acids (C14:0, C14:1, C16:1 and C18:1) were located in the BTA19 FASN gene located within this region but the FASN mutation had no significant effect on any traits. We also detected one significant SNP for C18:1 on BTA23 and two SNPs for C16:0 on BTA25. The region around 17 Mb on BTA26 harbored two significant SNPs for C14:1 and SNP in SCD in this region showed the strongest association with C14:1. This study demonstrated novel candidate regions in BTA19, 23 and 25 for fatty acid composition. 相似文献
187.
Takahiro Nishimaki Takayuki Ibi Yoshihiro Tanabe Yoshiyuki Miyazaki Naohiko Kobayashi Tamako Matsuhashi Takayuki Akiyama Emi Yoshida Kazumi Imai Mayu Matsui Keiichi Uemura Naoto Watanabe Tatsuo Fujita Yosuke Saito Tomohiko Komatsu Takahisa Yamada Hideyuki Mannen Shinji Sasazaki Tetsuo Kunieda 《Animal Science Journal》2013,84(8):585-591
Japanese Black cattle are at risk for genetic homogeneity due to intensive use of a few sires. Therefore, assessment of the actual genetic diversity of this breed is important for future breeding plans. In the present study, we investigated the genetic diversity within and among eight subpopulations of Japanese Black cattle using 52 microsatellite markers. The parameters for genetic diversity of Japanese Black cattle were comparable to those of other cattle breeds, suggesting that the relatively high genetic diversity of the breed. However, upon comparison among the eight subpopulations, the Hyogo subpopulation showed markedly low genetic diversity. The results of the pairwise FST values, phylogenetic network and structure analysis indicated that the Hyogo population has remarkably high level of genetic differentiation from other populations, while Yamagata, Niigata, Hiroshima and Kagawa populations have low levels of genetic differentiation. Furthermore, multidimensional scaling plots indicated that individuals in some subpopulations were separated from individuals in the other subpopulations. We conclude that while the overall genetic diversity of Japanese Black cattle is still maintained at a relatively high level, that of a particular subpopulation is significantly reduced, and therefore the effective population size of the breed needs to be controlled by correct mating strategies. 相似文献
188.
Hossam EL-SHEIKH ALI Go KITAHARA Youji TAMURA Ikuo KOBAYASHI Koichiro HEMMI Shidow TORISU Hiroshi SAMESHIMA Yoichiro HORII Samy ZAABEL Shunichi KAMIMURA 《The Journal of reproduction and development》2013,59(1):59-65
The aim of the present study was to describe the temperature of the different portions
of the female genital tract and their relation to rectal temperature and to investigate
the effect of steroid hormones profiles on these variables over the estrous cycle in
cattle. Four nonpregnant Japanese Black cows were investigated daily over two successive
estrous cycles using a digital thermometer with a long probe and rounded-end sensor to
record the temperature of the rectum (RT), vagina (VT), cervix (CT), uterine body (UBT)
and uterine horns (UHT). Blood samples were collected immediately before temperature
recording to assay peripheral levels of progesterone (P4) and estradiol-17β
(E2). Moreover, transrectal ultrasonography was carried out after temperature
recording to monitor the ovulatory follicle and track ovulation. During the experiment,
the ambient temperature and relative humidity were recorded for further calculation of the
temperature humidity index (THI). The temperature within the genital tracts in these cows
progressively increased towards the uterine horns from the vagina. The VT, CT, UBT and
UHTs were significantly higher in association with peripheral P4 concentrations
greater than 4 ng/ml (mid-luteal phase) when compared with lower peripheral P4
concentrations. The VT was more significantly (P<0.01) correlated to the CT, UBT and
UHTs than RT. In conclusion, a temperature gradient was present among the vagina, cervix
and uterus over the estrous cycle, and changes in peripheral P4 concentrations
were associated with the thermal variations within these portions. The VT could be more
beneficial than RT in monitoring temperature of deeper portions of the female genital
tract in bovine. 相似文献
189.
190.
Fumio NAKAMURA Iwao SEKI Ken KOBAYASHI Masakazu TANAKA Shigeharu FUKUNAGA 《Animal Science Journal》2002,73(6):553-556
A simple conventional method of immunohistochemistry (i.e. fixing the frozen sections in cold methanol) was used to determine the immunolocalization of cellular prion protein (PrPc), with good results. In the rat cerebrum, the cytoplasm of neural cells in the cortex and corpus stratum, pia mater, membrane limitans gliae superficialis, choroid plexus and blood vessel wall were immunostained. The formation of network structures of immunostained neural and/or glial fibers in the cerebral cortex was also observed. These immunostained network structures of neural and/or glial fibers were also observed in cultured neural cells. The results suggest that fixation of frozen sections and cultured cells with cold methanol is a useful method for detecting the immunolocalization of PrPc and that PrPc exists in the various components of the central nervous system of the rat. 相似文献