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11.
Misa HOSOE Nao YOSHIDA Yutaka HASHIYADA Hidetoshi TERAMOTO Toru TAKAHASHI Sueo NIIMURA 《The Journal of reproduction and development》2014,60(4):268-273
Fetal bovine serum (FBS) has been widely used as a supplement in the maturation medium of bovine oocytes in
vitro. However, serum contains many undefined factors and is potentially infectious to humans and animals. As a serum
replacement, we evaluated the feasibility of using the silk protein, sericin, derived from the cocoons of silkworm. To examine the
rates of oocyte maturation and fertilization, cumulus-oocyte complexes were cultured in TCM-199 supplemented with 0.01%, 0.05%,
0.1% or 0.15% sericin or 5% FBS. The sizes of the perivitelline space that might relate to polyspermy, the expressions of
Has2 and CD44 mRNA, the amount of hyaluronan (hyaluronic acid: HA) contained in the oocytes
and the rates of blastocyst formation following insemination were then compared between the oocytes cultured with 0.05% sericin
and 5% FBS, because the polyspermy rates in oocytes cultured with 0.05% sericin were significantly lower than in those cultured
with 5% FBS. After in vitro maturation (IVM), the mean size of the perivitelline space was significantly greater
in oocytes cultured with sericin than in those cultured with FBS, although the rates of nuclear maturation, fertilization and
blastocyst formation of oocytes under both IVM conditions were not significantly different. The expression of HAS2 and
CD44 mRNA and the amount of HA in the denuded oocytes cultured with 0.05% sericin were significantly greater than in
those cultured with FBS. These results indicate the feasibility of sericin as an alternative protein supplement for IVM in bovine
oocytes. 相似文献
12.
Fujiko MINAMI-FUKUDA Makoto NAGAI Hikaru TAKAI Toshiaki MURAKAMI Tadashi OZAWA Shinobu TSUCHIAKA Sachiko OKAZAKI Yukie KATAYAMA Mami OBA Naomi NISHIURA Yukiko SASSA Tsutomu OMATSU Tetsuya FURUYA Satoshi KOYAMA Junsuke SHIRAI Hiroshi TSUNEMITSU Yoshiki FUJII Kazuhiko KATAYAMA Tetsuya MIZUTANI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2013,75(12):1651-1655
13.
Katsuhiro OHKOSHI Seiya TAKAHASHI Shin-ichiro KOYAMA Satoshi AKAGI Noritaka ADACHI Tadashi FURUSAWA Jun-ichiro FUJIMOTO Yoshiaki IZAIKE Tomoyuki TOKUNAGA 《Animal Science Journal》2003,74(4):269-276
In vivo matured oocytes collected by laparoscopic follicular aspiration (LFA) from hormone treated female goats were used as recipient ooplasts for somatic cell nuclear transfer (SCNT). Japanese native (Shiba) goats were used as donor females and some donor females were used repeatedly (two or three times) at intervals of a few months. To induce synchronization of estrus, a sponge containing 0.5 g of progesterone was inserted into the vagina of each goat for 14 days. These animals were also treated with follicle stimulating hormone (FSH) in a series of 8 injections over 4 days. The first FSH injection was administered on the morning of day 9 of sponge insertion. On the morning of day 13, 50 µg of gonadotropin‐releasing hormone (GnRH) was injected into each animal. Twenty‐nine hours after GnRH injection, LFA was performed. After removal of cumulus cells, collected oocytes with the first polar body were selected and enucleated for nuclear transfer. Anterior pituitary cells isolated from an adult male Shiba goat were transfected with a DNA fragment containing the enhanced green flourescent protein gene and the puromycin resistance gene. A single donor cell was inserted into the perivitelline space of each enucleated oocyte and fusion was induced with one electric pulse of 20 V for 10 µs. The SCNT goat eggs were cultured in chemically defined medium at 38.5°C in 5% CO2, 5% O2, 90% N2 for 9 days. By LFA, 396 oocytes were collected from a total of 30 females. After removal of cumulus cells, 64% of them extruded the first polar body. The percentage of SCNT goat eggs produced using in vivo matured oocytes which developed to the blastocyst stage (20–21%) was significantly higher (P < 0.05) than that produced with in vitro matured oocytes (3–8%). 相似文献
14.
Randomized clinical trial to evaluate the effectiveness of enrofloxacin as a second‐line antibiotic for treatment of acute Escherichia coli mastitis 下载免费PDF全文
Kazuhiro Kawai Akira Takeda Michiaki Yamada Fumi Kayasaki Nao Kondo Yoshiko Sasaki Nahoko Kanai Takuma Mukai Naoki Sawaguchi Masaya Higuchi Haruka Kondo Kouki Sugimoto Akane Yasuda Aiko Watanabe 《Animal Science Journal》2018,89(7):1033-1039
The objective of the present study was to evaluate the effectiveness of enrofloxacin (ERFX) as a second‐line antibiotic for treatment of acute Escherichia coli (E. coli) mastitis. Forty‐two cows with naturally occurring acute E. coli mastitis were enrolled. On the first day of treatment (day 0), empirically selected antibiotics (oxytetracycline: n = 32, kanamycin: n = 10) were administered. Although systemic signs improved in 10 cows (first‐line group), the signs remained unchanged or worsened in 32 cows on day 1, including two cows that were found dead. The 30 surviving cows were randomly assigned to second‐line groups constituting an ERFX group (n = 19) or a control group (n = 11) that was treated with other antibiotics. Response to each treatment was evaluated by measuring clinical signs from day 0 to day 3, subsequent quarter milk recovery, and the 60‐day survival rate. Appetite on day 3 was significantly better in the ERFX group compared to the control group. No significant differences were observed in the 60‐day survival rate or the subsequent milk recovery between the ERFX group and the control group. Thus, the use of ERFX as a second‐line antibiotic for the treatment of acute E. coli mastitis could induce a rapid appetite recovery. 相似文献
15.
Methane uptake and nitrous oxide emission in Japanese forest soils and their relationship to soil and vegetation types 总被引:1,自引:1,他引:1
Tomoaki MORISHITA Tadashi SAKATA Masamichi TAKAHASHI Shigehiro ISHIZUKA Takeo MIZOGUCHI Yoshiyuki INAGAKI Kazuhiko TERAZAWA Satoshi SAWATA Masanori IGARASHI Hiroshi YASUDA Yasuhiro KOYAMA Yoshihito SUZUKI Nobuyuki TOYOTA Masamichi MURO Masaru KINJO Hirokazu YAMAMOTO Daitaro ASHIYA Yoichi KANAZAWA Tetsu HASHIMOTO Hidetaka UMATA 《Soil Science and Plant Nutrition》2007,53(5):678-691
16.
Jong-pil Seo Takashi Yamaga Nao Tsuzuki Kazutaka Yamada Shingo Haneda Hidefumi Furuoka Yasuhiko Tabata Naoki Sasaki 《The Canadian veterinary journal. La revue veterinaire canadienne》2014,55(11):1050-1056
This pilot study assessed the efficacy of 2 minimally invasive techniques for proximal interphalangeal (PIP) joint arthrodesis in horses. The PIP joints of both forelimbs (n = 6) were stabilized with locking compression plates (LCP) using a minimally invasive technique (LCP technique). Subsequently, for 1 randomly selected PIP joint of each horse, surgical drilling (SurD) was performed and tissue engineering (TE) was applied (LCP/SurD/TE technique). Minimally invasive PIP joint arthrodesis with LCP demonstrated low postoperative infection rates. Gross and histological evaluations revealed considerable destruction of the articular cartilage in the LCP/SurD/TE-treated joints. In contrast, almost no destruction of the cartilage was observed in the LCP-treated joints. Our results suggest that the LCP technique alone is not sufficient for PIP joint arthrodesis and that the LCP/SurD/TE technique may be useful for PIP joint arthrodesis in horses. 相似文献
17.
Shu HASHIMOTO Nao SUZUKI Ami AMO Takayuki YAMOCHI Yoshihiko HOSOI Yoshiharu MORIMOTO 《The Journal of reproduction and development》2013,59(5):496-499
Effects of supporting materials during vitrification procedure on the morphologies
of preantral follicles of pig ovaries were assessed. Ovarian cortical sections of
prepubertal pigs were randomly allocated to 5 groups. The sections were vitrified
ultrarapidly with 5 different vitrification devices. The sections were put on 4 fine
needles (Cryosupport), on a thin copper plate, or on a carbon graphite sheet or were
sandwiched between copper plates or between carbon graphite sheets before cooling.
The cooling and warming rates with the graphite sheets were significantly higher than
those with the copper plates (P<0.05). A total of 3,064 follicles were analyzed
following HE staining after vitrification with 5 different devices. The morphologies
follicles vitrified on the Cryosupport or on the graphite sheet were well preserved
compared with those vitrified on the copper plate or between copper plates
(P<0.01). The morphologies of follicles vitrified between copper plates were
mostly damaged (P<0.05). Taken together, good thermally conducting material
supports follicle morphologies of ovaries cryopreserved with ultrarapid
vitrification. 相似文献
18.
Yasuhiro Utsumi Shinya Koga Naoaki Tashiro Atsushi Yamamoto Yukie Saito Takanori Arima Hirokazu Yamamoto Masahiko Kadomatsu Nao Sakanoue 《Journal of Wood Science》2006,52(6):477-482
Of all plant materials used to cover the roofs of traditional Japanese buildings, Japanese cypress (Chamaecyparis obtusa) bark, hiwada, has the longest service life and has been used from ancient times. However, wood and bark properties after hiwada harvest have not been evaluated in detail. We studied whether decortication for hiwada production in winter affected xylem and phloem formation. Decorticated trees still preserved all inner bark and part of the
outer bark, and both decorticated and control trees had similar annual ring structures at all stem heights in the xylem and
phloem. In both xylem and inner bark, no significant difference in ring width at any stem height was found between annual
rings before and after decortication. Thus, this study revealed that the decortication of bark for hiwada production does not affect the formation of xylem and the inner and outer bark if decortication is carried out by highly
skilled workers in winter. 相似文献
19.
20.
Satoshi Taba Nao Miyahira Kanami Nasu Tetsuya Takushi Zen-ichi Moromizato 《Journal of General Plant Pathology》2007,73(5):374-376
Strawberry pear (pitahaya, pitaya) [Hylocereus undatus (Haw.) Britt. and Rose] postharvest fruit rot was found at an agricultural products store in Itoman city, Okinawa Prefecture
in 2006. The symptoms included depressed, water-soaked lesions with olive to black powdery spots coalescing into a soft rot.
The causal fungus was identified as Bipolaris cactivora (Petrak) Alcorn. This is the first report of strawberry pear fruit rot caused by B. cactivora. 相似文献