Lethal Effects of Pulsed High-Voltage Discharge on Marine Plankton and Escherichia coli

Ballast seawater is considered globally as a major vector for invasions of non-indigenous organisms. Several technologies have been tested for their ability to remove organisms from ballast water. In the present study, we constructed a novel pulsed high-voltage discharge (PHVD) system that could operate in either high current mode with several hundred amperes or shockwave generating mode with relatively lower current in seawater. In laboratory-scale experiments, the PHVD system with shockwave-generating mode was found to be more effective in killing zooplankton (1.9- to 4.0-fold) and phytoplankton (3.3-fold) than high current mode at discharge with 300–500 pulses at 7.1 kV. Further experiments were carried out at different voltages and pulse-numbers to examine effects of the shockwave-generating PHVD system on viabilities of one zooplankton larva, two phytoplankton species, and an indicator bacterium suspended in seawater in a static chamber. For zooplankton, live cells were not detected at discharge with 400 pulses at 13 kV. For phytoplankton, the initial live cells of a dinoflagellate was decreased by 77?±?0.5%, and the initial chl a concentration of a diatom was decreased by 76?±?6% at discharge with 700 pulses at 13 kV. For an indicator bacterium Escherichia coli, live cells were not detected at discharges with 200 or 700 pulses at 13 kV. Measurements of ATP content of organisms showed congruent results with those obtained by the above methods, suggesting it may be a rapid method for evaluating treatment efficiency. Though further scale-up studies are necessary, these results suggest that the PHVD system have a high potential for applying to ballast seawater treatment.     

Viable seeds and spores of weed species in peat soil under pineapple cultivation*

Soil samples were taken from twenty-two fields in a peat area in W. Malaysia where pineapples had been grown for different periods. The numbers of viable seeds and spores in the top 15 cm of fields under cultivation for 6 months, 2, 6 and 10 years varied from 66–122 millions/ha. Of this total, 90% were spores of Nephrolepis biserrata and eight other ferns. The total numbers of seeds of the two sedges, one grass and nine dicotyledons recorded, ranged from 0–7 to 6.3 millions/ha. Les semences et spores viables de mauvaises herbes en sol tourbeux dans des plantations d'ananas Des échantillons de sols ont été prélevés dans vingt-deux champs dans une zone tourbeuse en Malaisie occidentale oü les ananas ont été cultivés pendant des périodes différentes. Les nombres de semences et spores viables dans les 15 premiers centimétres du sol dans les champs mis en culture depuis 6 mois, 2, 6 et 10 ans ont varié de 66 ä 122 millions/ha. Sur ce total, 90 %étaient des spores de Nephrolepis biserrata et de huit autres fougéres. Le nombre total de semences des deux roseaux, de la graminee et des neuf dicotylédones dont la présence dut notée, fut compris entre, 0,7 et 6,3 millions/ha. Lebensfähigen Unkrautsamen und sporen im Moorboden unter Ananas In einem Moor in W. Malaysia wurden aus 22 Feldern die unterschiedlich lang unter Ananas standen, Bodenproben entnommen. Die Zahl an lebensfähigen Samen und Sporen in den obersten 15 cm der Felder, die 6 Monate, 2, 6 und 10 Jahre lang unter Ananas standen, schwankte von 66 bis 122 Millionen je ha. Von dieser gesamten Menge waren 90% Sporen von Nephrolepis biserrata und acht anderen Farnen. Die Gesamtzahl an Samen von zwei Seggen, einem Gras und neun dikotylen Arten, belief sich auf 0,7 bis 6,3 Millionen jeha.     

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Ohne Zusammenfassung     

Microbial dynamics and natural remediation patterns of Fusarium‐infested watermelon soil under 3‐yr of continuous fallow condition

Long‐term monoculture of watermelon results in inhibited growth and decreased crop yields, possibly because of imbalance in microbial ecology caused by accumulation of the pathogen in soil. This results in serious problems in the economics of watermelon production. We investigated the build‐up of Fusarium in soil under watermelon cultivation and changes over 3 yr of fallow in a microcosm. We focused on changes in the microbial community of Fusarium‐infected soil, including the diversity of the microfloral species composition, and species abundance. Long‐term monoculture of watermelon leads to changes in microbial diversity and community structure. The microbes most readily cultured from infested soil were suppressed by watermelon wilt pathogen Fusarium oxysporum f. sp. niveum (FON). Of 52 isolated and identified culturable microbes, 83.3% of bacteria, 85.7% of actinomycetes, 31.6% of fungi and 20.0% of Fusarium sp. were inhibited by FON on bioassay plates. Prior to fallowing, infested soil was a transformed ‘fungus‐type’ soil. After 3 yr of fallow, the infested soil had remediated naturally, and soil microbial diversity recovered considerably. Abundance of dominant bacterial populations was increased by 118–177%, actinomycetes, fungi and FON were decreased by 23–32, 33–37 and 50%, respectively. The ratio of bacteria: actnomycetes: fungus: Fusarium sp. in infested soil changed from 24 000:100:4:1 prior to fallow to 57 000:100:3.5:1 after fallowing, nearer to the 560 000:400:8:1 ratio of healthy soil not used for watermelon cultivation. This suggests the ‘fungus‐type’ soil was converting to ‘bacteria‐type’ soil and that disrupted microbial communities in infested soil were restored during fallow.     

Genotypic Differences in Root Hair Deformation and Subsequent Nodulation for Red Clover under Different Additions of Starter N Fertilization

Red clover cultivars, including diploid and tetraploid, are commonly used in legume‐based pasture mixtures. However, information on nodulation under different starter N regimens is limited. We hypothesized that there is genetic variability among different red clover cultivars for nodulation. A root hair deformation assay was conducted using three diploid (AC Christie, Tapani and CRS15) and three tetraploid (Tempus, CRS18 and CRS39) red clover cultivars by inoculating them with Rhizobium leguminosarum biovar trifolii. Nodulation and morphological characteristics of two selected red clover cultivars, AC Christie and Tempus, were determined under five starter N concentrations (0, 0.2, 0.4, 0.8 and 1.6 mg per plant). Inoculation with rhizobia increased root hair deformation with significant interaction across cultivars. Nodulation was delayed under high starter N concentrations, and genotypic differences were evident for days‐to‐nodule initiation. There was a positive quadratic response to starter N for AC Christie and a negative quadratic response for Tempus for nodulation. Tempus had more active nodules (92 %) than AC Christie (73 %). The genetic variability of red clover cultivars should be considered in N fixation studies and their response to availability of initial N.