Characterization of a galectin-like activity from the parasitic nematode, Haemonchus contortus, which modulates ovine eosinophil migration in vitro

The development of eosinophilia is a characteristic feature of helminth infection, although the exact nature of the interaction between eosinophils and parasites remains to be fully defined. Previously, it has been reported that Haemonchus contortus and other nematodes produce eosinophil-specific chemoattractants. This paper describes studies aimed at isolating and identifying the factor(s) responsible. Initial studies showed that soluble extracts of infective larvae (L3) of H. contortus provoked a chemokinetic, rather than chemotactic, response in ovine bone marrow eosinophils in vitro. This activity was inhibited by lactose to a markedly greater extent than sucrose suggesting a galectin-like identity. Lactose affinity chromatography of soluble H. contortus extracts resulted in the isolation a specific bound fraction which retained biological activity. SDS-PAGE gel electrophoresis indicated a single Coomassie-stained band at between 31 and 41kDa. Subsequent, mass spectrometric analysis confirmed that the bound fraction contained a mixture of nematode galectins. The results confirm that H. contortus larvae produce several galectin-like proteins, at least one of which demonstrates eosinophil chemokinetic activity in vitro. The possibility of the parasite-derived factor mimicking the mammalian galectin-9, a known eosinophil chemokine, is discussed.     

猕猴属实验动物的动物福利要求

为了确保猕猴属实验动物的顺利出口,满足国际上对实验用猴越来越高的动物福利要求,本文从制度建设,人员要求,以及饲养、隔离、运输环节的动物福利要求进行了分析,起草了一套完整的动物福利规范,供国内养殖企业和检验检疫部门参考。     

禄丰县生猪的疾病预防与治疗

近年来,禄丰县被云南省定为生猪调出大县,积极发展生猪养殖业,并积极采取各种措施助益生猪养殖产业又好又快发展,例如,出台生猪养殖优惠政策、引进优良生猪品种等。鉴于禄丰县良好的生猪养殖发展态势,为了提高生猪产量、保证生猪品质,加强对生猪疫病的防控显得尤其重要。现将禄丰县生猪常发的几种主要疫病及其防控措施调查介绍如下。     

Copper, zinc and manganese concentrations in equine liver, kidney and plasma

Five groups of horses were fed different diets of known trace mineral concentration for a minimum of six months. Copper (Cu), zinc (Zn) and manganese (Mn) concentrations were measured in livers of 125 yearling horses and kidneys of 81 yearling horses as an assessment of trace mineral status. Plasma Cu and Zn determinations were made for all horses.

Mean hepatic Cu concentrations of horses fed diets containing 6.9 to 15.2 mg Cu/kg dry matter (DM) feed were 0.27 to 0.33 μmol/g DM tissue. Plasma Cu concentrations ranged between 22.8 to 28.3 μmol/L. There was no simple mathematical relationship between plasma and hepatic Cu concentrations. Mean hepatic Zn concentrations in horses fed diets containing 25.6 to 52.2 mg Zn/kg DM feed were determined to be between 2.75 to 2.91 μmol/g DM tissue. Mean plasma Zn concentrations in groups of horses were between 11.7 to 13.5 μmol/L. Plasma Zn concentrations were not indicative of hepatic Zn concentration. Hepatic Mn concentrations ranged between 0.13 and 0.14 μmol/g DM tissue.

Renal Zn concentrations ranged between 1.55 to 1.63 μmol/g DM tissue and did not differ with diet. Mean renal Mn concentrations were 0.09 μmol/g DM tissue for all groups of horses. Renal Cu concentrations ranged from 0.36 to 0.47 μmol/g DM tissue and differed with diet.

     

农业信用担保“贷”动农业现代化

<正>我省试水农业信用担保,探索"财政、金融联动支农"新模式,通过担保"贷"动农业现代化。2010年,湖南农业信用担保有限公司实现担保融资8亿元,支持农业产业化龙头企业解决融资难题。中小企业融资难,"农"字号中小企业融资更     

茄子夏秋防虫网覆盖栽培技术

<正>茄子是夏秋主要蔬菜之一,但夏秋栽培易发生各种病虫害,特别是近年来随着设施园艺的发展,温室、连栋大棚、大棚等设施周年生产,白粉虱、烟粉虱等病虫害发生加重,防治难度加大,大量用药普遍,不但增加成本,还严重影响茄子的品质。为解决这一问题,2005～2007年我市推广茄子     

Microstructured optical fibers as high-pressure microfluidic reactors

Deposition of semiconductors and metals from chemical precursors onto planar substrates is a well-developed science and technology for microelectronics. Optical fibers are an established platform for both communications technology and fundamental research in photonics. Here, we describe a hybrid technology that integrates key aspects of both engineering disciplines, demonstrating the fabrication of tubes, solid nanowires, coaxial heterojunctions, and longitudinally patterned structures composed of metals, single-crystal semiconductors, and polycrystalline elemental or compound semiconductors within microstructured silica optical fibers. Because the optical fibers are constructed and the functional materials are chemically deposited in distinct and independent steps, the full design flexibilities of both platforms can now be exploited simultaneously for fiber-integrated optoelectronic materials and devices.