奶牛隐性子宫内膜炎诊断技术研究进展

奶牛隐性子宫内膜炎在以子宫内膜被嗜中性粒细胞(PMN)持续性的广泛浸润为主要特征,没有直观的临床症状,经常导致奶牛屡配不孕。在中国,由子宫内膜炎引起的奶牛不孕的比例达50.62%,且由隐性子宫内膜炎引起的奶牛不孕占其中的90%。由于隐性子宫内膜炎在发病初期不易被观察到,容易错过最佳治疗时间,造成巨大的经济损失,故如何对其做出正确、及时的诊断成了近年来的研究热点。目前,国内外奶牛隐性子宫内膜炎的诊断方法主要有阴道内窥镜法、细胞刷法、活体组织诊断法、超声诊断法及白血球酯酶法等,但目前这些诊断方法没有统一的判断标准。本文对几种不同的奶牛隐性子宫内膜炎的诊断方法进行了综述,为更早、更准确地诊断隐性子宫内膜炎提供依据。     

东港地区稻田适宜排水沟距试验对比分析

通过对辽宁东港灌区稻田排水沟距的综合对比试验分析，探讨了该地区稻田不同排水沟距对控制地下水位、渗漏量、土壤Eh值及水稻产量的影响，初步提出该地区稻田适宜的排水沟距。为今后该地区及类似地区排水沟道的规划设计提供了借鉴和参考，对水田的增产增收具有重要意义。     

内蒙古呼包鄂区域协调评价

以内蒙古呼和浩特市、包头市、鄂尔多斯市为研究对象,采用了层次分析法从经济水平、社会水平、资源环境3个方面构建指标体系,建立研究模型,对2010年呼和浩特市、包头市、鄂尔多斯市的区域系统发展程度进行了分析评价.     

Oral vaccination of brushtail possums with BCG: Investigation into factors that may influence vaccine efficacy and determination of duration of protection

AIMS: To determine factors that may influence the efficacy of an oral pelleted vaccine containing Mycobacterium bovis bacille Calmette-Guérin (BCG) to induce protection of brushtail possums against tuberculosis. To determine the duration of protective immunity following oral administration of BCG.

METHODS: In Study 1, a group of possums (n=7) was immunised by feeding 10 pellets containing dead Pasteur BCG, followed 15 weeks later with a single pellet of live Pasteur BCG. At that time, four other groups of possums (n=7 per group) were given a single pellet of live Pasteur BCG orally, a single pellet of live Danish BCG orally, 10 pellets of live Pasteur BCG orally, or a subcutaneous injection of live Pasteur BCG. For the oral pelleted vaccines, BCG was formulated into a lipid matrix, and each pellet contained approximately 10⁷ colony forming units (cfu) of BCG, while the vaccine injected subcutaneously contained 10⁶ cfu of BCG. A sixth, non-vaccinated, group (n=7) served as a control. All possums were challenged by the aerosol route with a low dose of virulent M. bovis 7 weeks after vaccination, and killed 7–8 weeks after challenge. Protection against challenge with M. bovis was assessed from pathological and bacteriological findings.

In Study 2, lipid-formulated live Danish BCG was administered orally to three groups of possums (10–11 per group), and these possums were challenged with virulent M. bovis 8, 29 or 54 weeks later. The possums were killed 7 weeks after challenge, to assess protection in comparison to a non-vaccinated group.

RESULTS: The results from Study 1 showed that vaccine efficacy was not adversely affected by feeding dead BCG prior to live BCG. Feeding 10 vaccine pellets induced a level of protection similar to feeding a single pellet. Protection was similar when feeding possums a single pellet containing the Pasteur or Danish strains of BCG. All vaccinated groups had significantly reduced pathological changes or bacterial counts when compared to the non-vaccinated group. In Study 2, oral administration of Danish BCG induced protection against challenge with M. bovis, which persisted for at least 54 weeks after vaccination. Some protection was observed in possums challenged 54 weeks after vaccination, but this protection was significantly less than that observed in groups vaccinated 29 or 8 weeks prior to challenge. There was a strong relationship between the proportion of animals producing positive lymphocyte proliferation responses to M. bovis antigens and protection against challenge with M. bovis.

CONCLUSIONS: Factors considered potentially capable of interfering with vaccination, including feeding dead BCG to possums prior to feeding live BCG, feeding multiple doses of BCG at one time, and changing strains of BCG, were shown not to interfere with the acquisition of protective immune responses in possums. Protection against tuberculosis was undiminished up to 29 weeks after vaccination with BCG administered orally. It is concluded that vaccination of possums by feeding pellets containing BCG is a robust and efficient approach to enhance the resistance of these animals to tuberculosis.     

内蒙古自治区区域协调问题与对策研究

内蒙古自治区与相关区域构成了一个复杂、开放的区域系统,其内部主要由自然区、行政区、主体功能区等组成;区域协调内容包括内蒙古自治区与全国的协调、内蒙古自治区东部与西部的协调、呼和浩特、包头、鄂尔多斯(简称呼包鄂,下同)三市协调、内蒙古自治区与俄罗斯等国家的协调、内蒙古自治区与相关省市自治区的协调、自然区与行政区的协调、行政区与主体功能区的协调、城市与农牧区的协调等;存在着许多社会经济指标低于全国平均水平、东部与西部社会经济等方面差距过大、出口依存度太低与外贸逆差过大、与相关省市自治区自然资源及产业的互补性差、地方利益与流域全局利益矛盾尖锐、主体功能区跨界缺乏统一领导、呼包鄂三市产业同构化现象趋于明显、城乡差距过大等问题;应该采取加强内蒙古自治区区域协调研究、编制区域协调规划、建立区域协调组织、利用调控手段促进区域协调发展和构建区域协调内在机制等对策.     

AMMI双标图在马铃薯区域试验产量分析中的应用

运用AMMI模型.结合双标图对2007年国家中晚熟西北组马铃薯品种区域试验各承试点的平均产量结果进行分析.试验结果表明,在参试品种中,L0031-17、青薯6号高产但不稳产,陇薯3号为高产稳产型,品种960102、中9931-48稳产但不高产,8025-1不稳产又不高产.AMMI模型具体、直观、有效的综合评价,为马铃薯品种提供了一种比较好的分析方法.     

马铃薯新品种青薯11号的选育

青薯11号是以同薯23号为母本,以青薯168为父本,通过有性杂交和系统选育育成的鲜食马铃薯新品种。中晚熟,出苗至收获107 d(天)左右;薯块椭圆形,粉红皮,薯肉黄色带红晕,芽眼浅;块茎淀粉含量17.96%,粗蛋白3.17%,VC240.3 mg·kg~(-1),还原糖0.279%。抗晚疫病。一般肥力条件下每667 m~2产量2 200~2 500 kg,高肥力条件下产量2 500~3 000kg。适宜在青海省各类地区种植。     

基于AMSR-2蒙古高原土壤水分反演及 对气象因子响应分析

土壤水分是地表水文过程研究的一个重要参数,是众多环境因子综合作用的结果,科学判定土壤水分对环境因子的响应特性,对在蒙古高原地区开展干旱监测预警,调整农业生产结构,改善区域生态环境具有重要意义。本研究基于AMSR-2观测亮温、SPOT-NDVI数据,利用微波辐射传输模型及粗糙地表发射率Qp模型,构建适合蒙古高原的土壤水分反演方程,同时将模型应用于2013年蒙古高原植被生长期土壤水分反演。在此基础上,结合TRMM 3B43降雨量及气象站点气温数据,探讨了蒙古高原土壤水分对气象因子及植被的响应特性。结果表明:1)构建的蒙古高原表层土壤水分反演模型精度较高,土壤水分反演值与实测值的判定系数为0.680 6,均方根误差(RMSE)达0.031 6 cm3·cm-3,反演结果明显优于JAXA提供的AMSR-2土壤水分产品数据(RMSE=0.044 1 cm3·cm-3)。2)TRMM 3B43降雨数据与实测降雨量线性拟合,其判定系数为0.859 8,直线拟合斜率K=0.941 5,在数值上较站点实测值略微偏低,表明TRMM 3B43数据精度较高,在蒙古高原具有很好的适用性。3)蒙古高原植被生长期土壤水分、植被指数及降水量在空间格局上均表现出由北向南、由东北向西南逐渐减少的趋势。干旱区,土壤水分对气温变化最敏感,二者表现出显著正相关关系,其次为降水和植被;半干旱区,植被是影响土壤水分的关键因子,而气温与降水对土壤水分影响呈现出季节性变化;半湿润区3个因子对土壤水分的影响程度表现为植被降水气温。总之,利用土壤水分对气象因子和植被的响应特性,可以采取适当措施降低蒙古高原灾害发生风险,为区域生态环境建设提供科学依据。     

漆酶基因异源表达及其酶活性的研究进展

近年来由于漆酶在生物漂白和农作物秸秆利用等方面具有广阔的应用前景,对漆酶的研究越来越受到国内外学者的重视。然而,自然界漆酶的产量和酶活较低,难以适应工业化生产需求。此外,漆酶的产酶效率低,成本高。实现漆酶的异源高效表达是解决这一问题的有效途径。目前,国内外已有多种来源的漆酶基因被成功克隆,并在不同的宿主细胞中实现异源表达,但迄今为止漆酶基因异源表达结果仍然不理想,离真正实现漆酶的高效表达还有一定距离。     

黑龙江省高校图书馆信息资源共建共享现状调查分析

信息资源共建共享是高校图书馆信息资源建设的重要内容。文章通过对黑龙江省高校图书馆信息资源共建共享现状进行调查和分析,总结信息资源共建共享存在的问题,并且提出实现黑龙江省高校图书馆信息资源共建共享的建设策略。