Duration of action and hemodynamic properties of mivacurium chloride in dogs anesthetized with halothane.

OBJECTIVE: To describe onset and duration of neuromuscular blockade induced by mivacurium chloride and its associated hemodynamic effects at 3 dosages in healthy dogs. ANIMALS: 7 Labrador Retrievers. PROCEDURE: Anesthesia was induced with thiopental and maintained with halothane in oxygen, and dogs were mechanically ventilated to end-tidal P(CO)2 between 35 and 40 mm Hg. Core temperature, end-tidal P(CO)2, and halothane concentration were kept constant throughout the experiment. Neuromuscular function was assessed by evaluation of the train-of-four response to a supramaximal electrical stimulus of 2 Hz applied to the ulnar nerve every 10 seconds. Blood for determination of plasma cholinesterase activity was obtained prior to administration of mivacurium, a bolus of which was administered IV, using a randomized Latin-square design for dosages of 0.01, 0.02, and 0.05 mg/kg of body weight. RESULTS: All dogs had typical plasma cholinesterase activity. After administration of mivacurium, differences were not evident between groups in heart rate, systolic, mean, or diastolic blood pressure, change at any time in heart rate, systolic, mean, or diastolic blood pressure, or pH. Interval from onset to 100% neuromuscular blockade was 3.92+/-1.70, 2.42+/-0.53, and 1.63+/-0.25 minutes at dosages of 0.01, 0.02, and 0.05 mg/kg, respectively. Duration of measurable neuromuscular blockade was 33.72+/-12.73, 65.38+/-12.82, and 151.0+/-38.50 minutes, respectively. Time of onset and duration of effect differed significantly among dosages. CONCLUSIONS AND CLINICAL RELEVANCE: Mivacurium provides good hemodynamic stability at the dosages tested. In dogs, this drug has a rapid onset and long duration of effect.     

Microsatellite variation between an African and five European taurine breeds results in a geographical phylogenetic tree with a bison outgroup

Genetic variation is being used extensively for individual identification and linkage analysis, and may be useful for interpopulation studies. Previously, blood groups and biochemical variants in blood cell and serum proteins have been used to study (evolutionary) relationships in mammals. But genetic divergence and gene flow among closely related populations are difficult to measure with these classical markers because their mutation rate is so low that new mutations have not had sufficient time to appear and become fixed. So they have a small number of alleles and a relatively low level of heterozygosity. These markers are now replaced by DNA markers, mostly microsatellites. These microsatellite loci are useful genetic markers at which alleles differ in length due to differences in the number of short sequence motifs arranged adjacent to one another. They are abundantly distributed throughout the mammalian genome. They have a large number of alleles, a high level of heterozygosity and are inherited in true Mendelian fashion. These characteristics make them valuable for parentage control, linkage analysis, genome mapping and phylogenetic studies. In terrestrial vertebrates with limited mobility, genetic differentiation often increases with the distance between populations or corresponds to the extent of geographic and habitat barriers (R oy et al. 1994). Investigations of short tandem repeats yield a considerable volume of genetic data regarding the similarities and divergence times of different cattle populations. Microsatellite markers are suitable for the estimation of these parameters as they are not generally subject to direct selection and environmental influences. Computation of genetic distances based on data from several loci can be used to evaluate the taxonomic relationship between populations. The aim of this study was to estimate the relative genetic variability between Belgian cattle breeds and to reconstruct the evolutionary relationship among them, also using two small genetically isolated cattle-like populations.     

A zinc-containing intraruminal device for facial eczema control in lambs

A zinc-containing intraruminal device has been developed for protecting lambs against facial eczema. The rate of release of zinc from the device has been optimised, and its safety in use established. Under both experimental and farm conditions, the device gave excellent protection against the liver injury associated with facial eczema. The device relies upon erosion for release of zinc, and disappears completely when its charge of zinc has been released, leaving no metal or plastic residue in the rumen. This device has the potential to greatly ameliorate the problem of facial eczema in New Zealand.     

Two cases of IgA multiple myeloma in the dog

Clinical and laboratory findings are presented from two cases of canine IgA multiple myeloma. One of these animals presented with typical clinical and pathological changes including haematological abnormalities and skeletal lesions with secondary invasion of other organs. In contrast, the primary focus of disease in the second dog was the anterior alimentary tract and there were no skeletal changes. In this respect this second case was similar to the condition in man known as alpha chain disease. The two paraproteins differed in electrophoretic mobility.     

Metabolism of microbial nitrogen in ruminants with special reference to nucleic acids

Characteristically the metabolism of microbial nitrogen (N) compounds in ruminants involves the degradation of dietary N and synthesis of microbial protein (MP), compounds including a small amount of peptides and free amino acids, which may account for 75–85% of total N and the remainder are nucleic acids (NA: DNA and RNA). Generally rumen microbes contain 10–25% NA‐N of the total N while 70–80% is in the form of RNA. This paper describes the degradation and synthesis of NA in the rumen and their fate in the lower digestive tracts. Their physiological and nutritional significance in different types of ruminant animals is also discussed. The research works on NA metabolism in ruminants has been mainly on metabolism of purines after rumen microbial digestion and absorption in the lower gut. Subsequently, the fate of absorbed purines has been intensively investigated to assess the extent of MP synthesis in the rumen. The method for predicting ruminal synthesized MP and subsequently digested MP has been proposed using urinary purine derivative (PD) excretion in sheep and cattle fed on ordinary feed. The latter approach has now been adopted for calculation of protein supply in some feeding standards, although there are still difficulties in predicting representative samples of rumen microbes, and also uncertainties in variations of non‐renal and endogenous purine losses.     

Use of ev loci as a measure of inbreeding in domestic fowls

1. The endogenous avian leukosis virus (ev) loci present in 9 lines of domestic fowls have been partially characterised and the average heterozygosity of the loci in each line calculated. 2. Using these data an estimate of the coefficient of inbreeding of the lines was derived; this estimate of the extent of inbreeding is compared with the mating history of the lines. 3. This method provides the first means of directly assessing the degree of inbreeding of fowl lines: assumptions implicit in the method are discussed.     

Monoclonal antibodies to sheep MHC class I and class II molecules: biochemical characterization of three class I gene products and four distinct subpopulations of class II molecules

The availability of a panel of monoclonal antibodies to sheep MHC class I and class II gene products has allowed for the first time an assessment of the relative complexity of the sheep MHC. By using four monoclonal antibodies to MHC class I, and seven monoclonal antibodies to MHC class II molecules together with one-dimensional SDS-PAGE, sequential immunoprecipitation and 2-dimensional gel analysis, three class I gene products and four distinct subsets of class II molecules have been identified. Sheep class I molecules showed heterogeneity on 2-dimensional gels and as in mouse and man, represented the products of at least three different non-allelic class I genes. Interestingly, the sheep beta 2 microglobulin molecule also displayed heterogeneity, consistent with either two primary gene products or allelic variation. Four sheep class II monoclonal antibodies identified distinct, non-overlapping subsets of sheep class II molecules of Mr 32-36 K (alpha chain) and 25-28 K (beta chain). These class II molecules were co-expressed on sheep B lymphocytes and represented the primary products of different sheep MHC class II genes. The class II molecules within three of these subsets displayed allelic polymorphism essentially restricted to their beta polypeptides, while the fourth subset of class II molecules showed allelic variation in both their alpha and beta polypeptides. The results of this study represent the first evidence for gene duplication and heterogeneity within the sheep MHC. The identification of three primary class I gene products and four distinct subsets of class II molecules suggests three class I loci and up to four distinct class II subregions within the sheep MHC. Potentially large numbers of allelic variants of these different gene products may be expressed in normal sheep.