Continuous Spectrophotometric Assay and Properties of Ascorbic Acid Oxidising Factors in Wheat

A continuous spectrophotometric assay was developed to measure ascorbic acid oxidation in crude Na₂SO₄ extracts of flour. The rate of ascorbic acid oxidation in flour extracts measured using this method was similar to the rate in flour-water suspensions and 2–4 fold less than the rate in dough measured using an indophenol-xylene extraction method. Flour extracts appeared to contain two ascorbic acid oxidising factors; one with optimal activity at pH 6·3 and 30 °C and the other with optimal activity at pH 10 and 40 °C. The pH 6·3 factor had properties similar to those of ascorbate oxidase (EC 1·10·3·3) in its pH and temperature stability, strong inhibition by NaN₃, KCN and diethyldithiocarbamate, inactivation by proteases, and greater stereospecificity towards -ascorbic acid than -isoascorbic acid. The pH 6·3 factor was most concentrated in the pollard milling fraction of wheat and was lowest in flour. The pH 10 factor had several properties indicating non-enzymic oxidation of ascorbic acid; it was not inactivated by proteases, it was inhibited poorly or not at all by the above ascorbate oxidase inhibitors, and it had low specificity for stereoisomers of ascorbic acid.     

不同生境下锌镉在伴矿景天不同叶龄叶中的富集与分布特征

田间试验、温室盆栽和生长室水培试验研究了Zn和Cd超富集植物伴矿景天不同叶龄叶片的Zn、Cd富集和分布特征。结果显示,田间条件下伴矿景天新叶中Cd浓度高于成熟叶、但新叶中Zn浓度低于成熟叶。温室盆栽试验发现,连续种植伴矿景天,第4季时低污染土壤（S1）上新叶中Zn浓度低于成熟叶,但高污染土壤（S4）上新叶中Zn浓度高于成熟叶;而到了第6季,S1、S4上新叶中Zn浓度均低于成熟叶,且在S1上两者达到显著水平;第4和第6季,伴矿景天新叶中Cd浓度始终高于成熟叶（除S1成熟叶中Cd浓度略高于新叶外）。水培试验结果发现,无论在高Zn（600 mmol/L）、高Cd（100 mmol/L）,还是低Zn（10 mmol/L）、低Cd（1 mmol/L）处理,新叶中Zn、Cd浓度均高于成熟叶;而且随着处理时间的延长,新叶和成熟叶中Zn、Cd浓度均明显上升。水培条件下,新叶和成熟叶中Zn最高分别为43 107、33 774 mg/kg（以干重计）（600 mmol/L,处理56 天）;新叶和成熟叶中Cd的最高浓度分别为15 057、9 060 mg/kg（以干重计）（100 mmol/L Cd,处理56 天）。这些结果表明,Zn、Cd在伴矿景天新叶和成熟叶中富集和分布与其生长介质中Zn、Cd的浓度尤其是有效态浓度、处理时间及植物所处的生长阶段等有关,同时也表明Zn、Cd优先分布于新叶可能与伴矿景天超富集Zn、Cd的机制有关。     

The structural state of buried and surface soils of solonetzic complexes in the dry steppe zone of the Lower Volga basin

The structural state of modern (surface) soils and the soils buried under Anna Ioannovna??s rampart (1718?C1720) was studied. These soils are the components of solonetzic soil complexes in the southern Privolzhskaya Upland. The dehumification and the high content of calcium in the exchange complex determine the state of the macrostructure of the chestnut soil buried about 300 years ago. The dehumification drastically lowers the water stability of the soil aggregates, and the predominance of calcium ions in the soil exchange complex prevents the destruction of the chestnut paleosol aggregates and preserves their aggregate state upon moistening. For the last 300 years, no significant changes in the macrostructure of the solonetzes have been observed.     

小麦品种的耐盐性变化：甜菜碱和乙烯的作用

Four wheat (Triticum aestivum L.) cultivars 711, PBW343, 3765 and WH542 were screened for studying variations in glycinebetaine (GB) content and plant dry mass under 100 mmol L-1 NaCl stress. A tolerance index was calculated using plant dry mass data to select salt-tolerant and salt-sensitive types and find association between tolerance index and GB content. Tolerance index has been used as a good criterion to select the tolerant types under high salinity stress. Further, physiological differences in salt-tolerant cultivar 711 and salt-sensitive cultivar WH542 were examined. The salt-tolerant cultivar exhibited greater GB content, which was found correlative with ethylene. The cultivar also showed higher nitrogen (N) content and nitrate reductase activity, reduced glutathione and higher redox state resulting in maximal protection of plant dry mass than the salt-sensitive type. Thus, the content of GB may be considered as important physiological criteria for selecting salt-tolerant wheat types.     

果园巡检机器人长臂抖动抑制方法

针对果园巡检机器人长臂因抖动而严重制约图像采集效率的问题,该研究提出基于臂体上3个部位的仰角构建单一反馈量进行抖动抑制的方法。首先基于臂体外伸段的有限元模态分析结果,通过固有频率和静变形的等效,建立三杆两扭簧等效机构的动力学模型。并基于微分平坦理论将3个倾角传感器读数合成为一个系统输出量,以平坦输出量作为被控量,采用PID控制器进行抑振试验,再利用自抗扰控制(Active Disturbance Rejection Control, ADRC)思想中对广义扰动的处理方法,将被控模型等效为二阶系统形式,并将模型信息代入到扩张状态观测器中进行对比试验。试验结果表明,基于本文构建的方法均能起到快速抑制长臂抖动的作用。在冲击作用致使末端产生10°幅值的抖动情况下,PID控制器实现了7 s内抑制大幅抖动,自抗扰控制器实现了8 s内快速抑制大幅抖动的效果。PID控制器的输出出现3次饱和现象且小幅较高频率抖动不易消除,而自抗扰控制器仅出现一次饱和,具有抖动抑制过程平滑的优点。本文的控制算法简洁适于在单片机等微型计算机系统中应用,系统结构简单成本低,可为现代农业装备研发与应用中解决长臂类结构抖动的主动抑制问题提供理论参考。     

山东省畜禽粪便的环境污染现状及风险评价

以耕地和水环境保护为出发点,根据山东省各地市近五年来畜禽养殖量数据,采用排泄系数法估算畜禽粪便的产生量,在此基础上计算了畜禽粪便的耕地负荷和水体等标污染指数等指标,综合评价了各地市畜禽养殖业的发展对土壤和水环境产生的污染压力。研究发现：2014-2018年山东全省畜禽粪便的平均耕地污染风险指数为0.77~0.84,预警级别为Ⅲ级;全省平均水体等标污染指数由9.26逐渐降低为4.46,对应的预警级别由Ⅱ级降为Ⅰ级。表明畜禽污染已对土壤环境产生风险,潍坊市尤为突出;而畜禽污染对水体环境基本无污染风险,但不同地区间存在差异,聊城市的水体污染风险仍相对较高。此外,依据土壤养分平衡理论和山东省耕地面积估算,山东省2018年畜禽的最高承载数量（以猪当量计）为12 873万头。本研究为优化畜禽养殖结构、推动区域畜禽养殖业绿色发展、保护区域生态可持续发展提供了理论参考。     

Computed tomography of testicular torsion in a juvenile dog with unilateral cryptorchidism

A 14-week-old male unilaterally cryptorchid Clumber spaniel was presented for acute lethargy. Physical examination revealed abdominal pain, and a single testis was palpated in the scrotum. Abdominal ultrasound and computed tomography (CT) revealed a poorly vascularized, ovoid structure immediately caudal to the left kidney with scant regional peritoneal effusion. Left intra-abdominal testicular torsion was confirmed at surgery, and routine cryptorchidectomy was performed. The patient recovered uneventfully from anesthesia and surgery.Key clinical message:The most common CT characteristics of testicular torsion were present in this case and correlated well with sonographic findings to allow for rapid, accurate diagnosis and surgical planning of unilateral, non-neoplastic, intra-abdominal cryptorchid testicular torsion in a juvenile dog. Contrast enhanced CT facilitated accurate localization of the undescended testis and evaluation of testicular perfusion and may be a useful alternative to ultrasound for diagnosing testicular torsion, especially in indeterminate cases.     

施钾对兰州百合叶片抗旱性生理指标的影响

采用田间试验,研究了钾肥对兰州百合开花期叶片抗旱生理指标的影响.结果表明,施钾量在0~150kg/hm2范围内,随着钾肥施入量的增加,丙二醛含量降低,可溶性糖含量、过氧化氢酶活性和脯氨酸含量增加,但施钾量超过150 kg/hm2时,丙二醛含量略有上升,可溶性糖含量、过氧化氢酶活性和脯氨酸含量有所下降.在施氮量225 kg/hm2和施磷量150 kg/hm2的不打顶处理下叶片丙二醛含量低于其它处理,脯氨酸含量、过氧化氢酶活性和可溶性糖含量高于其它处理;且在施氮量225 kg/hm2、施磷量150 kg/hm2和施钾量150 kg/hm2的不打顶处理下,丙二醛含量出现最小值,脯氨酸含量、过氧化氢酶活性和可溶性糖含量呈现最高值.     

The cell and molecular basis of mechanical, cold, and inflammatory pain

Peripheral pain pathways are activated by a range of stimuli. We used diphtheria toxin to kill all mouse postmitotic sensory neurons expressing the sodium channel Nav1.8. Mice showed normal motor activity and low-threshold mechanical and acute noxious heat responses but did not respond to noxious mechanical pressure or cold. They also showed a loss of enhanced pain responses and spontaneous pain behavior upon treatment with inflammatory insults. In contrast, nerve injury led to heightened pain sensitivity to thermal and mechanical stimuli indistinguishable from that seen with normal littermates. Pain behavior correlates well with central input from sensory neurons measured electrophysiologically in vivo. These data demonstrate that Na(v)1.8-expressing neurons are essential for mechanical, cold, and inflammatory pain but not for neuropathic pain or heat sensing.     

Transformation of the nitrogen cycle: recent trends, questions, and potential solutions

Humans continue to transform the global nitrogen cycle at a record pace, reflecting an increased combustion of fossil fuels, growing demand for nitrogen in agriculture and industry, and pervasive inefficiencies in its use. Much anthropogenic nitrogen is lost to air, water, and land to cause a cascade of environmental and human health problems. Simultaneously, food production in some parts of the world is nitrogen-deficient, highlighting inequities in the distribution of nitrogen-containing fertilizers. Optimizing the need for a key human resource while minimizing its negative consequences requires an integrated interdisciplinary approach and the development of strategies to decrease nitrogen-containing waste.