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41.
Endogenous opioid suppression of release of luteinizing hormone during suckling in postpartum anestrous beef cows 总被引:1,自引:0,他引:1
Beef cows were used to determine if suckling influences release of LH via endogenous opioids at 28 +/- 4 d after parturition. Cows of similar weight and body condition (6.8 +/- .1, 1 = emaciated, 9 = obese) were assigned randomly to five groups (n = 6 to 7): 1) control-suckled/saline (suckled 15 min every 6 hr for 48 hr); 2) control-suckled/naloxone; 3) calf-removal/saline (calf removal for 52 hr); 4) calf-removal/naloxone; and 5) control-suckled/GnRH (Gonadotropin-Releasing Hormone). At 0 hr, saline was administered to all cows. This treatment was continued at 6 hr intervals for 24 hr. Either naloxone (0.5 mg/kg), GnRH (40 ng/kg) or saline was administered to cows in their respective groups every 6 hr during the ensuing 24-hr period in calf-removal groups, or immediately preceding each suckling episode in the control-suckled groups. Blood samples for analysis of luteinizing hormone (LH) were collected at 15-min intervals for 1 hr prior to and 3 hr after treatment at 0, 24, 36 and 48 hr. Cows were observed for estrus twice daily. All cows in the control-suckled/GnRH group released LH (P less than .05) in response to exogenous GnRH, indicating the presence of releasable quantities of the gonadotropin. Mean concentrations of LH were not effected (P greater than .05) by the control-suckled regime. However, calf-removal alone, or in combination with naloxone, increased (P less than .05) mean concentrations of LH by 48 hr.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
42.
Norman Myers 《Forest Ecology and Management》1983,6(1):59-79
Tropical moist forests (TMFs) are undergoing three principal types of exploitation — commercial logging, large-scale ranching and small-scale cultivation — that generally amount to unsustainable use. These modes of exploitation, or rather over-exploitation, focus on only a very few of the many products available from the forest ecosystems, resulting in degradation if not destruction for the rest. Through systematic screening of raw materials such as phytochemicals and genetic resources for industry, agriculture, and medicine, we could make expanded use of tropical forests — and the harvesting of these low-volume products need cause little disruption of forest ecosystems. Moreover, tropical forests offer many environmental services, whose value is increasingly apparent as deforestation proceeds. A comprehensive development strategy is required if we are to make best use of these forests, with a shift in emphasis from short-term, narrow-interest exploitation of a few products, to long-term, broad-scale utilization of whatever goods and services can be made available without degradation of the ecosystem. 相似文献
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Jane A. Fagerland Ronald K. Myers Lawrence H. Arp 《Veterinary immunology and immunopathology》1994,40(4):367-377
The uptake of macromolecular and particulate materials in bronchus-associated lymphoid tissue (BALT) in turkeys was examined using transmission electron microscopy. Tracer materials used were live and ultraviolet-killed (UV-killed) Bordetella avium and ferritin. Suspensions of bacteria and ferritin were instilled via intratracheal catheterization and allowed to remain in contact with the respiratory surfaces for 0, 10, 30, 60, 90, and 120 min. Ferritin and B. avium were taken up by both ciliated and non-ciliated cells of the epithelium overlying BALT (BALT epithelium). Ferritin was found in organelles associated with endocytosis (i.e. apical vesicles, endosomes, cytoplasmic vacuoles) and was apparently transported across epithelial cells, since it was also found in intercellular spaces. Bacteria were found in vacuoles within BALT epithelial cells, but not free in intercellular spaces. Some macrophages in BALT epithelium also contained bacteria. No differences were observed between uptake of live and UV-killed bacteria. We conclude that both ciliated and non-ciliated cells of BALT epithelium in turkeys are able to take up macromolecular and particulate materials. Bacteria are also accessible to intraepithelial macrophages, although whether they are taken up directly from the bronchial surface or whether they pass through epithelial cells first could not be determined. This evidence suggests that antigens, including respiratory pathogens, could gain access to cells of the avian immune system by transepithelial passage in BALT. 相似文献
48.
In 2002, the Kentucky Farm Tractor Overturn Survey was administered, in which 6,063 randomly selected farm operators responded to questions that addressed the presence and use of seatbelts in the event of a tractor overturn. Data were analyzed to determine the proportion of seatbelt presence and use on tractors that overturned differentiated by whether they were equipped or not equipped with a rollover protective structure (ROPS). In 537 overturns reported for which the ROPS status was known, 92 involved ROPS-equipped tractors, of which 60 had a functioning seatbelt, and 19 operators of these tractors used the seatbelt that was present during the overturn. However, of the 445 overturns of non-ROPS tractors, eight had a functioning seatbelt, and three of these operators wore the seatbelt. Two of the three operators that wore seatbelts on non-ROPS tractors suffered a permanent disability. In contrast, of the 19 operators who wore a seatbelt on ROPS-equipped tractors, 18 experienced no or minor injuries, and one required outpatient care. Seatbelts are known to save lives but are a secondary safety device to ROPS, for ROPS alone saves lives and is a necessary pre-condition for seatbelt presence and use. 相似文献
49.
Peters SM Yancy H Deaver C Jones YL Kenyon E Chiesa OA Esparza J Screven R Lancaster V Stubbs JT Yang M Wiesenfeld PL Myers MJ 《Veterinary immunology and immunopathology》2012,148(3-4):236-242
Non-steroidal anti-inflammatory drugs (NSAID) are a family of chemicals that function to reduce pain, fever, and inflammation, and they are commonly used in people and animals for this purpose. Currently there are no NSAIDs approved for the management of inflammation in swine due to a lack of validated animal models and suitable biomarkers to assess efficacy. A previous in vitro study examining biomarkers of inflammation identified fourteen genes that were significantly altered in response to Escherichia coli lipopolysaccharide (LPS)-induced inflammation. In the present study, five of those fourteen genes were tested in vivo to determine if the same effects observed in vitro were also observed in vivo. Plasma levels of prostaglandin E(2) (PGE(2)), an essential mediator of fever and inflammation, were also determined. Two groups of swine were stimulated with LPS with the second group also treated with flunixin meglumine. Blood was collected at 0, 1, 3, 6, 8, 24, and 48h post LPS-stimulation. The RNA was extracted from the blood and quantitative real-time-PCR (qRT-PCR) was utilized to determine the expression patterns of CD1, CD4, serum amyloid A2 (SAA2), Caspase 1, and monocyte chemoattractant protein 1 (MCP-1). The LPS-stimulated animals demonstrated a statistically significant alteration in expression of SAA2 and CD1 at 3h post-stimulation. Flunixin meglumine treated animals' demonstrated reduced expression of CD1 in comparison to the LPS-stimulated swine at 24 and 48h post LPS-stimulation. Flunixin meglumine treated animals exhibited reduced expression of SAA2 at 48h post-stimulation compared to LPS-stimulated swine. Swine treated with LPS demonstrated statistically significant increases in plasma PGE(2) at 1h post-stimulation. Swine treated with flunixin meglumine had no increase in plasma PGE(2) levels at any time. These results demonstrate that PGE(2) production, along with two out of five genes (SAA2 and CD1) have the potential to serve as early biomarkers of inflammation as well as indicators of NSAID efficacy. 相似文献
50.
Kyle L. Tofflemire R. David Whitley David M. Wong Kenneth R. Waller III Ronald K. Myers Angela E. Pillatzki Gil Ben‐Shlomo 《Veterinary ophthalmology》2013,16(2):149-152
An 11‐year‐old Oldenburg mare presented following three episodes of acute, transient blindness, ataxia, and disorientation within the preceding 7 months. Clinical improvement, including return of vision, occurred within 1 week of initiating corticosteroid therapy for each of the three episodes. However, mild right‐sided miosis was a consistent finding on ophthalmic examinations. Routine clinicopathologic testing revealed no significant abnormalities, and testing of cerebral spinal fluid for selected infectious diseases was unrewarding. Computed tomography of the brain demonstrated a hyperattenuating mass with peripheral mineralization in the rostroventral aspect of each lateral ventricle. The mare was euthanized due to a guarded to poor prognosis. On histopathology, the masses consisted of clusters of cholesterol clefts admixed with leukocytes, mineral deposits, and connective tissue. Cholesterinic granulomas of the lateral ventricles and hydrocephaly were diagnosed. Cholesterinic granulomas should be considered a differential diagnosis in horses presenting for intermittent blindness. 相似文献