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The genomes of human, mouse, and rat have been sequenced. Now, as O'Brien and Murphy announce in their Perspective, the genome sequence derby is heating up with the addition of dog to the list (Kirkness et al.). As they explain, even though the coverage of the dog genome (1.5x) is lower than that of mouse (8x), there are many valuable insights to be gained from comparing the sequence of dog with those of mouse and human. 相似文献
84.
Murphy WJ Larkin DM Everts-van der Wind A Bourque G Tesler G Auvil L Beever JE Chowdhary BP Galibert F Gatzke L Hitte C Meyers SN Milan D Ostrander EA Pape G Parker HG Raudsepp T Rogatcheva MB Schook LB Skow LC Welge M Womack JE O'brien SJ Pevzner PA Lewin HA 《Science (New York, N.Y.)》2005,309(5734):613-617
The genome organizations of eight phylogenetically distinct species from five mammalian orders were compared in order to address fundamental questions relating to mammalian chromosomal evolution. Rates of chromosome evolution within mammalian orders were found to increase since the Cretaceous-Tertiary boundary. Nearly 20% of chromosome breakpoint regions were reused during mammalian evolution; these reuse sites are also enriched for centromeres. Analysis of gene content in and around evolutionary breakpoint regions revealed increased gene density relative to the genome-wide average. We found that segmental duplications populate the majority of primate-specific breakpoints and often flank inverted chromosome segments, implicating their role in chromosomal rearrangement. 相似文献
85.
Teeling EC Springer MS Madsen O Bates P O'brien SJ Murphy WJ 《Science (New York, N.Y.)》2005,307(5709):580-584
Bats make up more than 20% of extant mammals, yet their evolutionary history is largely unknown because of a limited fossil record and conflicting or incomplete phylogenies. Here, we present a highly resolved molecular phylogeny for all extant bat families. Our results support the hypothesis that megabats are nested among four major microbat lineages, which originated in the early Eocene [52 to 50 million years ago (Mya)], coincident with a significant global rise in temperature, increase in plant diversity and abundance, and the zenith of Tertiary insect diversity. Our data suggest that bats originated in Laurasia, possibly in North America, and that three of the major microbat lineages are Laurasian in origin, whereas the fourth is Gondwanan. Combining principles of ghost lineage analysis with molecular divergence dates, we estimate that the bat fossil record underestimates (unrepresented basal branch length, UBBL) first occurrences by, on average, 73% and that the sum of missing fossil history is 61%. 相似文献
86.
Improved surface temperature prediction for the coming decade from a global climate model 总被引:9,自引:0,他引:9
Smith DM Cusack S Colman AW Folland CK Harris GR Murphy JM 《Science (New York, N.Y.)》2007,317(5839):796-799
Previous climate model projections of climate change accounted for external forcing from natural and anthropogenic sources but did not attempt to predict internally generated natural variability. We present a new modeling system that predicts both internal variability and externally forced changes and hence forecasts surface temperature with substantially improved skill throughout a decade, both globally and in many regions. Our system predicts that internal variability will partially offset the anthropogenic global warming signal for the next few years. However, climate will continue to warm, with at least half of the years after 2009 predicted to exceed the warmest year currently on record. 相似文献
87.
Ullah SM Murphy B Dorich B Richter B Srinivasan K 《Journal of agricultural and food chemistry》2011,59(6):2169-2174
This paper describes a new in-cell method for pursuing accelerated solvent extraction (ASE) prior to lipid analysis from food samples. It is difficult to pursue direct ASE with acid- or base-hydrolyzed samples due to the corrosive nature of the reagents and material limitations. In this study ion exchange based materials were used to remove acid or base reagents in-cell without compromising the recovery of lipids. The performance data are presented here for the new methods for lipid extraction for a variety of food samples and compared to the Mojonnier method. NIST Standard Reference Materials (SRM-1546 and SRM-1849) were used to validate the ASE methods. Excellent fat recoveries were obtained for the ASE methods. The new methods presented here enhance the utility of ASE and eliminate labor intensive protocols. 相似文献
88.
Dissolved organic matter (DOM) has been recognised as a key carbon and nitrogen (N) pool involved with soil-plant-microbe interactions. Yet few studies have quantified this contribution in agricultural soils. In this study we leached DOM from a sandy loam and sandy clay loam soil under either grassland or arable cropping. Two weeks after DOM removal microbial respiration from soils was not altered. However, a significant (P<0.05) decline in microbial biomass-N, potentially mineralizable-N, gross N mineralization and gross nitrification occurred after leaching. This data illustrate that whilst DOM is a small component of the soil OM it contributed up to 25% of microbial N supply within these agricultural soils. 相似文献
89.
Hubert DJ Ullrich DR Murphy TH Lindner JR 《Journal of agricultural safety and health》2001,7(3):143-153
The purpose of this study was to gather benchmark data for the assessment of the knowledge, attitudes, and perceptions regarding agricultural safety issues and curricula held by Texas agricultural teachers with less than two full years of teaching experience (entry-year teachers). Seventy-four of 118 well-distributed teachers responded to this survey. Researchers concluded that more females were entering a traditionally male-dominated field. Overall, teachers addressed safety within units of instruction rather than as separate units. The most useful forms of new teaching resources that this group of teachers would like to see produced were safety videos and study guides, and class demonstration/simulation activities. There was a significant difference in rankings between teachers less than 26 years old and teachers more than 26 years old regarding the usefulness of transparencies as a new teaching resource (F = 5.00, p = 0.0268). Few teachers were currently CPR and first aid certified, even though most had received training and completed a general safety and/or health related course while in college. Teachers generally agreed philosophically with most practices and exhibited personal beliefs consistent with proper safety preparedness and practice in agricultural settings. However, many of these teachers failed to practice what was expected of safe tractor operators, such as wearing safety belts and allowing younger drivers to operate the equipment. 相似文献
90.