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A. Fulton B. Arzi B. Murphy D. K. Naydan F. J. M. Verstraete 《Veterinary and comparative oncology》2014,12(4):258-265
Oral squamous cell carcinoma (OSCC) and canine acanthomatous ameloblastoma (CAA) represent two epithelium‐derived neoplasms that affect the oral cavity of dogs. The expression of cytokeratins (CKs) and calretinin has been previously established in the canine tooth bud and odontogenic tumours. The aim of this study was to characterize the CK and calretinin expression profile of OSCC in comparison to CAA and canine tooth bud tissues. Samples from 15 OSCC and 15 CAA cases, as well as 6 tooth buds and 2 normal gingival tissues were examined. OSCC CK expression was consistent with the CK expression profile of CAA and canine tooth bud tissue. Calretinin was positively expressed in 10 of 15 OSCC cases, with 5 cases demonstrating high staining intensity. Only 2 of 15 CAA cases demonstrated mild‐moderate staining intensity. The statistically significant difference in staining pattern and intensity of calretinin in OSCC and CAA can help distinguish between these two tumour types. 相似文献
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C. R. C. Sheppard K. Matheson J. C. Bythell P. Murphy C. Blair Myers B. Blake 《水产资源保护:海洋与淡水生态系统》1995,5(4):277-298
- 1 The nature and distribution of the coastal and shallow marine habitats of Anguilla in the Caribbean were examined using aerial photography coupled with extensive ground truth information.
- 2 The objective was to develop an accurate method of marine habitat mapping for the analysis of the marine ecosystem in order to develop a procedure for marine and coastal resource management.
- 3 This paper describes the method which was adopted as well as the biological results.
- 4 It is concluded that the management and sustainable use of the natural resources may be improved as a result of accurate habitat mapping.
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Michael W. Mullowney Eoghainín ó hAinmhire Anam Shaikh Xiaomei Wei Urszula Tanouye Bernard D. Santarsiero Joanna E. Burdette Brian T. Murphy 《Marine drugs》2014,12(6):3574-3586
As part of our program to identify novel secondary metabolites that target drug-resistant ovarian cancers, a screening of our aquatic-derived actinomycete fraction library against a cisplatin-resistant ovarian cancer cell line (OVCAR5) led to the isolation of novel diaza-anthracene antibiotic diazaquinomycin E (DAQE; 1), the isomeric mixture of diazaquinomycin F (DAQF; 2) and diazaquinomycin G (DAQG; 3), and known analog diazaquinomycin A (DAQA; 4). The structures of DAQF and DAQG were solved through deconvolution of X-Ray diffraction data of their corresponding co-crystal. DAQE and DAQA exhibited moderate LC50 values against OVCAR5 of 9.0 and 8.8 μM, respectively. At lethal concentrations of DAQA, evidence of DNA damage was observed via induction of apoptosis through cleaved-PARP. Herein, we will discuss the isolation, structure elucidation, and biological activity of these secondary metabolites. 相似文献
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Greg I Coman Peter I Crocos Stuart J. Arnold Sandy I. Keys Nigel P. Preston Brian Murphy 《Journal of the World Aquaculture Society》2005,36(4):464-479
The growth, survival and reproductive performance of domesticated Australian stocks of the giant tiger shrimp Penaeus monodon were evaluated in trials conducted in 1997 and 2003. The 1997 trials assessed the performance of first generation progeny of wild broodstock from the northeast coast of Australia and fourth generation progeny of pond‐reared broodstock, which also originated from northeast coast wild stocks. In these trials, growth and survival of the shrimp were assessed when reared for 17 mo in tanks. Reproductive performance of the shrimp was assessed at 14.5 mo and 17 mo. The 2003 trials assessed the performance of first generation progeny of wild broodstock from the Gulf of Carpentaria (north coast of Australia). In these trials, growth and survival of shrimp were assessed when reared for 14 mo in tanks and raceways. Reproductive performance of the shrimp was assessed at 11 mo, 12 mo, and 15 mo. Growth and reproductive performance of the stocks varied between trials, families, ages and rearing systems. The most pronounced differences in growth and reproductive performance were between the 1997 and 2003 trials. At 11 mo of age, the average wet weight of the shrimp in the 2003 trials (females 117.1 ± 5.8 g; males 87.9 ± 7.6 g) was 200% greater than the average wet weight of shrimp in the 1997 trials (females 55.2 ± 6.8 g; males 41.2 ± 3.4 g). The reproductive performance of the shrimp was also higher in the 2003 trials in terms of the percentage of spawnings hatching (52.0% in 1997; 77.1% in 2003) and mean hatch rate (21.5% in 1997; 31.6% in 2003). Differences in the growth and reproductive performance of the tank‐reared stocks between years were indicative of significant improvements in the rearing environment, diet and husbandry techniques. Variation in the reproductive performance between families was consistent across rearing environments and at different ages and suggests the potential to improve reproductive performance through genetic selection. Notably, this study identified hatch rates of nauplii from the spawned eggs as a key area for future improvement of domesticated stocks reared in tanks and raceways. Future efforts to improve the growth and reproductive performance of domesticated P. monodon could benefit from integrating incremental improvements to husbandry with genetic selection. 相似文献