弱光条件下东方百合的生长发育及光合特性研究

为了探明弱光条件下东方百合的生长发育及光合特性,为我国北方地区东方百合切花的耐弱光品种选育提供依据,以东方百合品种‘Marcopolo’、‘Tiber’、‘Constanta’和‘Acapulco’为试验材料,在整个生长季对其进行不同遮光处理(正常光照、50%遮光、75%遮光),测定不同光强下各品种光合参数及生长发育指标。结果表明:75%遮光条件下东方百合各品种的净光合速率(Pn)、光补偿点(LCP)、光饱和点(LSP)、暗呼吸速率(Rd)降低,表观量子效率(AQY)下降,叶绿素含量增高,叶绿素a与叶绿素b的比值降低,比叶质量减小,叶面积增大,切花观赏品质有所下降;不同百合品种的耐弱光性不同,弱光下‘Marcopolo’的LCP、Rd较低,比叶质量较小,AQY变化幅度小,表现出较强的耐弱光性;相反地,‘Acapulco’则对弱光较敏感。     

四种大黄属高山药用植物蒽醌类成分含量的测定

为比较掌叶大黄、喜马拉雅大黄、菱叶大黄、头序大黄4种高山药用大黄中蒽醌类成分含量的差异,采用紫外分光光度法,以大黄素为对照品,0.5%醋酸镁-甲醇溶液为显色剂,测定了样品溶液在510nm处的吸光度。结果表明,4种大黄中总蒽醌和结合蒽醌的含量为喜马拉雅大黄掌叶大黄菱叶大黄头序大黄,游离蒽醌的含量为喜马拉雅大黄掌叶大黄头序大黄菱叶大黄,与藏药用大黄的等级划分基本一致。     

芥蓝菜薹不同节间芥子油苷组分及含量分析

采用高效液相色谱及液相色谱-质谱联用法,以芥蓝(Brassica alboglabra L.)3个品种‘中花’、‘澄海粗条,和'全年油青’为材料,对菜薹不同节间(菜薹从膨大处到顶端依次平分为4节)芥子油苷组分和含量进行了鉴定和分析.结果表明,除4-甲硫基丁基芥子油苷在全年油青菜薹的第1节中没有检测到、4-羟基-3-吲哚...     

Use of the phenolsulphonphthalein dye test for fallopian tube patency in cattle

Results of preliminary investigations of fallopian tube patency in cattle using a method based on intrauterine instillation of PSP dye, and detection of the dye in the urine, suggest that this test can provide a useful diagnostic aid in cases of bilateral occlusion. In such cases PSP dye is not evident in the urine two hours later. In normal animals dye is present within 30 minutes. In two animals with unilateral blockage dye appeared at an intermediate time between the normal and bilaterally occluded cases. Repetition of the test and the use of adequate volumes of dye to cause uterine distension may be necessary to eliminate false negatives.     

Preferential synthesis of ferritin and albumin by different populations of liver polysomes

Free polysomes and a mixture of free and membrane-attached polysomes were isolated separately from rat liver, and each was added to a cell-free, protein-synthesizing system. The free polysomes showed a greater capacity than the mixed polysome population for incorporation of (14)C-leucine into ferritin, whereas the reverse was true for (14)C-leucine incorporation into albumin.     

A comparison of the interferon gamma assay with the absorbed ELISA for the diagnosis of Johne''s disease in cattle

Two interferon gamma (IFN-gamma) assays, the IFN-gamma enzyme immunoassay (EIA) and the IFN-gamma bioassay and an absorbed ELISA were used to screen 6 cattle herds for Johne's disease. Each herd had a history of Johne's disease but the majority of infected animals did not show clinical signs. The disease status of the cattle, which were removed from the herds, was confirmed by bacteriological culture of faeces or histopathological examination and culture of tissues collected at necropsy. The sensitivities of the IFN-gamma assays and the absorbed ELISA were determined using test results from infected animals. The sensitivity of the IFN-gamma EIA in detecting subclinical (71.8 to 93.3%) and clinical animals (100%) was not significantly different. However, the IFN-gamma bioassay and the absorbed ELISA were more sensitive in detecting cattle with advanced infections (80%) than those that were subclinically affected (16.7 to 33.3%).