Validation of liquid chromatographic method for assay of calcitriol and alfacalcidol in capsule formulations

The validation of a liquid chromatographic procedure suitable for the determination of calcitriol and alfacalcidol in their respective formulations labeled to contain at least 0.25 micrograms drug per unit is described. The capsule content is diluted and chromatographed in 15-20 min on silica columns (5 micron) with a mobile phase of hexane-tetrahydrofuran-methylene dichloride-isopropanol (72 + 12 + 12 + 4, v/v) with detection at 254 nm. The calibration curve is linear. Recoveries of "spikes" averaged 101% with a standard deviation of 2%. Precision was better than 1.5%.     

Venus: detailed mapping of maxwell montes region

From October 1983 to July 1984, the north hemisphere of Venus, from latitude 30 degrees to latitude 90 degrees , was mapped by means of the radar imagers and altimeters of the spacecraft Venera 15 and Venera 16. This report presents the results of the radar mapping of the Maxwell Montes region, one of the most interesting features of Venus' surface. A radar mosaic map and contour map have been compiled.     

Effects of evisceration and storage temperature on changes in meat quality of traditional farm‐fresh Turkeys

1. Conditions affecting the keeping quality of traditional farm‐fresh turkeys were investigated.

2. Storage of uneviscerated Wrolstad turkeys at 4 °C for 10 days caused no statistically significant changes in meat flavour or texture.

3. During further storage at —2 °C, however, there was a slight but significant change in flavour, which became more marked with time in birds which had been eviscerated after the initial period at 4 °C.

4. Both eviscerated and uneviscerated birds became slightly tougher during storage.

5. Initial holding at 4 °C increased the numbers of psychrotrophic bacteria on the skin by about 10³ but subsequent changes at — 2 °C were slight for uneviscerated birds.

6. Eviscerated carcases had higher counts than uneviscerated birds after storage at — 2 °C and, although ‘off’ odours were not detected, spoilage appeared to be imminent at the end of the 20‐d period.     

Book reviews

Birds Their Structure and Function, By A. S. King and J. McLelland, 1984. 2nd ed. 334 pp. illustrated. London, Baillière Tindall. £9.50. ISBN 0 7020 0872 9.

Proceedings of the 2nd European Symposium on Poultry Welfare, 1985. 360 pp. illustrated. German Branch of the World's Poultry Science Association, Celle, Institut für Kleintierzucht.     

A toxic dipeptide from the defense glands of the colorado beetle

The Colorado beetle is protected against predators by the secretions of defensive glands located on the pronotum and the elytra. The single major compound of the secretion was identified as gamma-L-glutamyl-L-2-amino-3(Z),5-hexadienoic acid by spectroscopic and chemical methods. This compound, which contains a nonprotein beta,gamma- unsaturated amino acid, is toxic to ants (Myrmica rubra) at a concentration 10(-2) molar, which is less than its estimated concentration in the secretion (1.8 x 10(-1) molar).     

Optical experiments and weak interactions

Recent optical experiments have demonstrated cases in which mirror symmetry in stable atoms is broken during absorption of light. These results, which are in contradiction with quantum electrodynamics, support the theory of unification of the electromagnetic and weak forces. The interpretation of these experimental results is based on exchanges of weak neutral Z(0) bosons between the electrons and the nucleus of the atom. The information obtained from low-energy experiments is different from, but complementary to, the results of high-energy experiments. Sensitive measurements in a simple, reliably computable atom are in quantitative agreement with the standard electroweak theory and put stringent constraints on alternative models. Attaining sufficient accuracy in the experiments and the computations for the electroweak radiative corrections to manifest themselves is now the challenge for experimenters and theorists.