Evaluation of limb autotomy as a promising strategy to improve production performances of mud crab (Scylla olivacea) in the soft‐shell farming system

Soft‐shell crab, a newly moulted crab having non‐calcified and hydrated soft exoskeleton, farming has been increasing tremendously in recent years throughout the world due to its high market price and consumer preferences. However, high aggressiveness and cannibalistic behaviour of mud crab limit its culture to an individual chamber system which ultimately makes the soft‐shell mud crab farming space and labour‐intensive. Therefore, the aim of this study was to improve the efficiency of soft‐shell crab production by autotomy of the claw and/or walking legs to reduce the moulting duration and intensify the culture system through double stocking in the existing individual chamber culture system. To achieve our objectives, a series of experiment were conducted at both indoor and on‐farm levels by removing the claw and/or walking legs of Scylla olivacea for two consecutive moulting cycles. Results showed that both claw ablation significantly (p < .01) shortened the moulting duration and increased the carapace width, weight gain, specific growth rate, feeding efficiency and yield parameter compared to the non‐ablated crabs more prominently at 1st moulting than the 2nd moulting in both indoor and on‐farm trials. Ablation of all walking legs while keeping the claw intact did not significantly influence (p > .05) the moulting duration and carapace width, but significantly (p < .01) improved the specific growth rate, weight gain, feeding efficiency and yield parameters at 1st moulting compared to the non‐ablation and unilateral three walking leg ablation groups. However, ablation of all appendages (both claw and walking legs) showed significant (p < .01) improvements of carapace width, weight gain, specific growth rate, feeding efficiency and yield parameters, and shortened the moulting duration up to 23 days from 34 days during 1st moulting and up to 27 days from 40 days at 2nd moulting. The survivals of different autotomized mud crabs were statistically non‐significant (p > .05) to the non‐autotomized crabs in various indoor and on‐farm trials. Besides improving the production performances, ablation of bilateral claw and all appendages allowed to double the stocking density in the existing individual chamber‐based soft‐shell crab farming that enhanced the production about threefold higher than the existing individual chamber‐based culture system. Finally, our results suggest that autotomy could be a promising technology for the commercial farming of soft‐shell mud crab in near future.     

Zingiber officinale and Glycyrrhiza glabra,individually or in combination,reduce heavy metal accumulation and improve growth performance and immune status in Nile tilapia,Oreochromis niloticus

This study evaluated the impact of dietary ginger and liquorice supplementation on growth performance, physiological and histopathological profiles and heavy metal accumulation in Nile tilapia fingerlings. Fish (n = 1,800, 17.5 ± 0.11 g BW) were randomly distributed into four treatment groups in triplicates and received no supplementation (control group), 5 ml aqueous ginger extract/kg feed (ginger group), 4 ml aqueous liquorice extract/kg feed (liquorice group) or 2.5 ml ginger plus 2 ml liquorice aqueous extracts/kg feed (mix group). The ginger‐liquorice mix supply improved the growth performance and feed efficiency (p < .05), increased the haematocrit and haemoglobin (p < .05), leucocytes (p = .108), neutrophils (p = .054), serum total protein (p < .05), albumin (p = .011) and globulin (p = .094) but decreased (p < .05) the blood urea nitrogen and creatinine than feeding liquorice or ginger lonely compared to the control. Heavy metal loads in pond water induced lamellar telangiectasis of gills and necrosis with sloughing of intestinal villi tips. These detrimental effects were alleviated, and the intestinal villus length (p = .041) and crypt depth (p = .069) were increased with liquorice supply. In all treatment groups, heavy metal contents in fish flesh were lower compared to the control. Thus, using ginger and/or liquorice aqueous extracts can decrease heavy metal accumulation in the fish flesh and exert positive effects on growth performance, metabolic profile and the intestinal and gill morphology of Nile tilapia.     

Temperature and plant age drive downy mildew disease epidemics on oilseed <Emphasis Type="Italic">Brassica napus</Emphasis> and <Emphasis Type="Italic">B. juncea</Emphasis>

Studies were undertaken on the effects of temperature (14/10 °C and 22/17 °C day/night) and plant age (15, 23, 31 and 40 day-old-plants) on the severity of downy mildew (Hyaloperonospora parasitica) on oilseed Brassica cultivars (temperature: Brassica juncea Montara, B. napus Atomic, ATR-Hyden, Hyola 432, Hyola 450 TT, Thunder TT; plant age: B. juncea Dune, B. napus Surpass 402 and Hyola 450 TT). For temperature studies, there were significant (P?<?0.001) effects of temperature, cultivar, and cultivar x temperature interaction. On cotyledons of susceptible cultivars (B. napus Hyola 450 TT and Thunder TT), plants were symptomatic at 22/17 °C by 48 h post inoculation (hpi) and with abundant sporulation evident by 72 hpi, and with all cotyledons of B. napus Thunder TT collapsed by 7 days post inoculation (dpi). However, at 14/10 °C, there were no symptoms on the same cultivars until 5 dpi, and sporulation only observed at 7 dpi. Percent disease index values (DI%) at 22/17 °C of B. juncea Montara and B. napus ATR-Hyden, Hyola 432, Atomic, Hyola 450 TT and Thunder TT were 4.5, 49.0, 51.4, 65.8, 86.3 and 96.0, respectively, with all except B. juncea Montara having significantly lower (P?<?0.001) disease at 14/10 °C with DI% values of 2.8, 30.4, 27.9, 31.1, 44.4 and 76.4, respectively. For plant age studies, there were significant (P?<?0.001) effects of plant age, cultivar, and cultivar x plant age interaction. DI% was significantly higher at 15 compared to 40 day-old-plants (dop) across all cultivars. B. juncea Dune showed greatest resistance, particularly on 40 dop, with DI% values of 25.8, 24.6, 22.9 and 7.5, for 15, 23, 31 and 40 dop, respectively. B. napus Surpass 402 showed high susceptibility on cotyledons of 15 dop but moderate resistance on leaves of other ages, with DI% values of 59.0, 31.2, 27.1 and 26.2 for 15, 23, 31 and 40 dop, respectively. B. napus Hyola 450 TT showed very high susceptibility at the cotyledon stage on 15 dop, but some resistance on 23 dop and more so on 31 and 40 dop, with DI% values of 84.0, 41.2, 35.4 and 32.9 for 15, 23, 31 and 40 dop, respectively. Together, these findings explain for the first time why development of downy mildew epidemics on susceptible cultivars occurs early in the growing season when warmer seasonal temperatures in autumn coincide with presence of seedlings; in contrast to later in the growing season on less susceptible older plants coinciding with cooler and less favourable winter temperatures. Increasing maximum and minimum temperatures associated with climate change have likely fostered the increased severity of downy mildew over the past 15 years.     

In vitro propagation of conifers using mature shoots

Micropropagation mostly leads to the production of innumerable true-to-type plants. However, establishing pathogen-free explants through in vitro culture requires a precise management of time for the exposure of explants to antimicrobial chemicals. The application of antimicrobial chemicals must also be managed to impose the least injury on explants. This review discusses the contributions of micropropagation procedures, explant types, subculture duration, media ingredients and plant growth regulators to the in vitro response of conifer explants. Even though regeneration from mature conifer explants such as mature shoots are laborious, the chances of variation, induced in vitro, are unlikely.     

The potential for combining living wall and vertical farming systems with aquaponics with special emphasis on substrates

Aquaponics is a method of food production, growing fish and vegetables in a recirculating aquaculture system. Aquaponics uses the water from the fish to feed the plants in a totally natural way and like hydroponics, aquaponics is considered to be more sustainable as more plants can be grown per square metre compared to normal agriculture. However, as is the case with normal agriculture, in aquaponics plants are grown within horizontally. In aquaponics, using the UVI system, the ratio between fish tanks:filters:plant tanks is 2:1:5 which means that the plant tanks are occupying close to half of the production space. In order to reduce the spatial requirement for plants, which would make production even more sustainable, this research investigates aspects of combining living wall and vertical farming technologies in aquaponics. It is considered that by growing the plants vertically less space would be required. In this research living wall system is investigated but the main focus is on the potentials of using various inert substrates in the living wall systems for vertical aquaponics. The results showed that a pot system performs better in terms of management of the systems. With regard to substrates, horticultural grade coconut fibre and horticultural grade mineral wool outperformed other substrates.     

Optimization of the stocking parameters for mud spiny lobster Panulirus polyphagus (Herbst, 1793) capture‐based aquaculture in tropical open sea floating net cages

Mud spiny lobsters, Panulirus polyphagus (Herbst, 1793), were reared at four different stocking sizes and stocking densities in open sea cages to evaluate their effects on growth performance. To evaluate the effect of stocking size on the growth performance, the lobsters were segregated into four different treatment groups according to size and were stocked at a density of 300 animals per cage. To evaluate the effect of stocking density on the growth performance, lobsters of 81–100 g were stocked in four different stocking densities, i.e. 16/m², 24/m², 32/m² and 40/m². The results showed that the growth rate of (60–80 g) size group, was significantly higher compared to the size groups, i.e. 101–120 g and 121–140 g comprising of larger individuals. The final body weight, though significantly higher in 81–100 g as compared to 60–80 g, the growth performance (i.e. weight gain percentage (WG %) and specific growth rate (SGR)) were not significantly different. The density‐dependent influence on growth performance was evident in this study. The WG % and SGR during 90 days’ culture period was significantly higher in 24/m² compared to other groups. This study provides crucial information about the appropriate stocking density and stocking size of lobsters at the field level, which would help to promote sustainable lobster cage farming by maximizing the production potential of the system.     

Copper functionalization of polypropylene fabric surface in order to use in fog collectors

Polypropylene fabrics were coated with copper particles using electroless plating, screen printing and wire arc spray coating techniques. Surface morphology of the fabrics was studied using optical and scanning electron microscopes (SEM). Furthermore, tensile strength, electrical conductivity, thermal conductivity, air permeability, water contact angle and fog collection efficiency of the coated fabrics were measured and the obtained results were analyzed. SEM micrographs showed that a very thin and uniform layer of copper deposited on the surface of the electroless plated polypropylene fibers. In the printed or spray coated fabrics the copper particles filled the spaces between yarns and fibers. The polypropylene electroless copper plated fabrics showed higher tensile strength, electrical conductivity, air permeability and thermal conductivity when compared with the fabrics coated with copper screen printed and copper spray coated fabrics. Finally, the obtained results showed that copper electroless plating could increase the fog collection efficiency of polypropylene fabrics considerably. As a conclusion, the surface modified copper electroless polypropylene fabrics are good candidates for fog collection in appropriate regions which need further investigations.     

Effects of partial replacement of fish meal with Spirulina platensis meal in practical diets on growth,survival, body composition,and reproductive performance of three-spot gourami (Trichopodus trichopterus) (Pallas, 1770)

This study investigated the effects of fish meal replacement by Spirulina platensis meal on growth, survival, body biochemical composition, and reproductive performance of Trichopodus trichopterus until the first spawning for 16 weeks. The fish fed dietary algal meal at levels of 2.5, 5, 10, and 20 % against those received fish meal only as control. The results showed that indicators such as belly diameter, feed intake, feed conversion ratio (FCR), and viscerosomatic index were significantly different between treatments with 5 and 10 % algal replacements (P < 0.05). The growth factors including final weight, weight gain, total length, fork length, and body height of fish did not show significant differences (P > 0.05) between the treatments. Feed intake increased up to 10 % replacement level and then decreased, though the best FCR values (0.77–0.79) were obtained with 2.5 and 5 % substitutions. The body lipid content showed a statistically marked decrease (P < 0.05) as a result of the algal replacements, but the amounts of protein and moisture were not significantly (P > 0.05) different between treatments. All fish fed S. platensis meal had greater gonadosomatic indices (19.4–21.85 %) than that of the control in spite of insignificant differences (P > 0.05) in their ovary weights (2.07–2.21 g). Absolute fecundity ranged between 7300 and 12,700 eggs/female with the highest amount in fish fed with replaced S. platensis levels of 2.5–10 %. The second-order polynomial regression analysis between absolute fecundity and substitution levels of algal meal was y = ?0.5319x ² + 106.87x + 7812.9 (R ² = 0.9642), and the optimal substitution level was determined to be 8.13 % for maximum absolute fecundity. Hatching percentages in the control and 20 % replacement were much lower (P < 0.05) than those in the other treatments (78–86 %). Given the relative enhancement of growth parameters and significant improvement in reproductive performance of broodstock three-spot gourami, 8.1–9.6 % S. platensis meal instead of fish meal is recommended in the diets.     

Response of wheat growth and productivity to exogenous polyamines under lead stress

Polyamines [i.e. spermine (Spm), spermidine (Spd) and putrescine (Put)] antagonistic effects on stress imposed by 2.0 mM lead (Pb²⁺) on growth, yield and its components and changes in the osmoprotectant and endogenous Pb²⁺ concentrations, the contents of some nutrients and tissue health in wheat plants were evaluated. Under the three applied polyamine (PAs) applications, the efficiency of wheat plants to tolerate Pb²⁺ stress in terms of growth and yield characteristics was noticed to varying degrees. The enhancements in osmoprotectant concentrations and plant health [in terms of relative water content (RWC) and membrane stability index (MSI)], and reductions in electrolyte leakage (EL) and plant Pb²⁺ concentration were correlated with the reasonable growth of Pb²⁺-stressed plants and their grain yield. Results point out that, better growth and yield characteristics, MSI, RWC, leaf photosynthetic pigment and osmoprotectant concentrations, and nutrient contents were obtained with seed soaking in 0.25 mM Spm, 0.50 mM Spd or 1.0 mM Put than those generated with seed soaking in water under 2.0 mM Pb²⁺ stress. In contrast, EL and the concentration of endogenous Pb²⁺ were significantly reduced. However, the Pb²⁺-free control positively exceeded the all stressed treatments. Among all tested PAs, 1.0 mM Put showed the best results and thus is recommended, as seed soaking, for wheat to grow well under Pb²⁺ stress.